BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140025.11 + phase: 0
(217 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g15260 unknown protein 207 3e-54
At3g01170 unknown protein 174 4e-44
At3g06180 unknown protein 116 8e-27
At5g19020 putative protein 114 5e-26
At4g29560 unknown protein 36 0.014
At4g00360 probable cytochrome P450 32 0.34
At1g62305 putative protein 30 0.75
At1g02530 hypothetical protein 30 0.98
At2g01950 putative receptor protein kinase 30 1.3
At5g23660 MtN3-like protein 28 4.8
At5g41850 unknown protein 27 8.3
At1g08070 unknown protein 27 8.3
>At5g15260 unknown protein
Length = 234
Score = 207 bits (528), Expect = 3e-54
Identities = 125/238 (52%), Positives = 155/238 (64%), Gaps = 25/238 (10%)
Query: 1 MVNFHSLISSVDQSSSSMANSIDL--------AKSRNRKTLNSLQIPQ----CERSRSAV 48
MV F + IS VDQS MA+S D S NRK+ +S C+ SRSA
Sbjct: 1 MVYFQNSISLVDQSPI-MAHSPDFHHHSKQSRTSSSNRKSSSSSCSSNFSVCCDGSRSAA 59
Query: 49 VDVVIFIAVVIALGFLVFPYIQFVMSESFKLCGLFMDLVKEEVA--VAPVIYVSLGVSVS 106
+DVVI IAV+ + GFL+FPYI+F+ +S ++ LVKEE+ P++Y + +S+S
Sbjct: 60 IDVVILIAVITSSGFLIFPYIKFITIKSVEIFSELSCLVKEEILRNPDPIVYGLIALSIS 119
Query: 107 CAVVATWFFIAY--TSRKCSNPNCKGLKNA-AEFDIQLETEDCVKNSPSLGKDGGGIKKG 163
C ++ W + + +C PNCKGL+ A AEFDIQLETEDCVK+S S GK KKG
Sbjct: 120 CTALSAWMIVILLCSRHRCGKPNCKGLRKANAEFDIQLETEDCVKSSNS-GKSVS--KKG 176
Query: 164 LFKIPCDHHRELEAELKKMAPVNGRAVLVLRGKCGCSVGRLEVPGPKK----NRKIKK 217
LF++P DHHRELEAELKKMAP NGRAVLV R KCGCSVGRLEVPGPKK RK+KK
Sbjct: 177 LFELPRDHHRELEAELKKMAPPNGRAVLVFRAKCGCSVGRLEVPGPKKQQLQQRKVKK 234
>At3g01170 unknown protein
Length = 596
Score = 174 bits (440), Expect = 4e-44
Identities = 97/202 (48%), Positives = 129/202 (63%), Gaps = 15/202 (7%)
Query: 1 MVNFHSLISSVDQSSSSMANSIDLAKSRNRKTLNSL-----QIPQCERSRSAVVDVVIFI 55
MV HS IS V S M S KSRN + +S + P C+ S+SA +DVVI I
Sbjct: 1 MVYLHSSIS-VCNSVDPMIMSHSSPKSRNTRNKSSSSPTCSKFPVCDGSQSAAIDVVILI 59
Query: 56 AVVIALGFLVFPYIQFVMSESFKLCGLFMDLVKEEVAVAPVIYVSLGVSVSCAVVATWFF 115
AV+ A GFL FPY++ + +S ++ LVK+E+ P++Y SL +S+ CA ++TW
Sbjct: 60 AVITACGFLFFPYVKLITLKSIEVFSDLSLLVKQEILQNPIVYGSLALSIFCAAISTWLV 119
Query: 116 IAY-TSRKCSNPNCKGLKNAAEFDIQLETEDCVKNSPSLGKDGGGIKKGLFKIPCDHHRE 174
I T ++C PNCKGL+ A EFDIQLETE+CVK+S + K+G+F++P HHRE
Sbjct: 120 ILLCTMQRCGKPNCKGLRKAVEFDIQLETEECVKSSSN--------KRGMFELPRVHHRE 171
Query: 175 LEAELKKMAPVNGRAVLVLRGK 196
LEAELKKMAP NGRAVLV R +
Sbjct: 172 LEAELKKMAPPNGRAVLVFRAR 193
>At3g06180 unknown protein
Length = 241
Score = 116 bits (291), Expect = 8e-27
Identities = 79/221 (35%), Positives = 121/221 (54%), Gaps = 13/221 (5%)
Query: 5 HSLISSVDQSSSSMANSIDLAKSRNRKTLNSLQIPQCERSRSAVVDVVIFIAVVIALGFL 64
HS +S+ SSS+ ++ + + S + + +SL + C+ S SA +D++I I V+ + FL
Sbjct: 12 HSTTNSMPPSSSASRSASNHSSSSSSSS-SSLHL--CKHSPSATLDLLILILVLFSGAFL 68
Query: 65 VFPYIQFV------MSESF-KLCGLFMDLVKEEVAVAPVIYV-SLGVSVSCAVVATWFFI 116
+ Y ++ +S F L L ++ ++ P Y + V + ++
Sbjct: 69 LSSYFSYLFHSFSLLSSHFPSLSSLIFSDDEDLSSIPPASYFFAFAVFFAASIAFLDLCC 128
Query: 117 AYTSRKCSNPNCKGLKNAAEFDIQLETEDCVKNSPSLGKDGGGIKKGLFKIPCDHHRELE 176
SRKC NP CKGLK A EFD+QL+TE+CVK+ + D K G P + L
Sbjct: 129 GPRSRKCRNPKCKGLKKAMEFDLQLQTEECVKSGATKEIDRLPWKGGSESNP--DYECLR 186
Query: 177 AELKKMAPVNGRAVLVLRGKCGCSVGRLEVPGPKKNRKIKK 217
AEL++MAP NGRAVL+ R +CGC V +LE GPK+ R+ KK
Sbjct: 187 AELRRMAPPNGRAVLLFRSRCGCPVAKLEGWGPKRGRRHKK 227
>At5g19020 putative protein
Length = 939
Score = 114 bits (284), Expect = 5e-26
Identities = 83/240 (34%), Positives = 124/240 (51%), Gaps = 34/240 (14%)
Query: 6 SLISSVDQSSSSMANSIDLAKSRNRKTLNSLQIPQCERSRSAVVDVVIFIAVVIALGFLV 65
S ++V+ +S++M S A ++N +S + C+ S SA +D++I I V+ + FL+
Sbjct: 12 STTTAVEDNSTTMPPSSRSAANQN----SSSSLHLCKHSPSATLDLLILILVLFSGTFLL 67
Query: 66 FPYIQFVMSESFKLCGLFMDLVKEEVAVAP--VIYVSLGVSVS----------CAVVATW 113
Y +++ L F + ++ P +I+ S S A++
Sbjct: 68 SSYFSYLIHSLSLLSSHFPSITISLSSLLPPLIIFFSSDHSTEDEDHHHPSGKIPPPASF 127
Query: 114 FF---------IAYT-------SRKCSNPNCKGLKNAAEFDIQLETEDCVKNSPSLGKDG 157
FF IA+ SRKC NP CKG+K A EFD+QL+TE+CVK+ D
Sbjct: 128 FFAFAVFFAASIAFLDLCCGSRSRKCRNPKCKGMKKAMEFDLQLQTEECVKSGSVKEIDR 187
Query: 158 GGIKKGLFKIPCDHHRELEAELKKMAPVNGRAVLVLRGKCGCSVGRLEVPGPKKNRKIKK 217
K G P + L AEL+KMAPVNGRAVL+ R KCGC + +LE GPK++R+ KK
Sbjct: 188 LPWKGGSESNP--DYECLRAELRKMAPVNGRAVLIFRSKCGCPIAKLEGWGPKRSRRHKK 245
>At4g29560 unknown protein
Length = 493
Score = 36.2 bits (82), Expect = 0.014
Identities = 23/71 (32%), Positives = 38/71 (53%), Gaps = 6/71 (8%)
Query: 11 VDQSSSSMANSIDLAKSRNRKTLNSLQIPQCERSRSAVVDVVIFIAVV------IALGFL 64
+DQ+S+ ++S A NR + SL QC+++ S V V+FI + L FL
Sbjct: 25 LDQASNLFSSSSSAAAPINRSSFVSLLKKQCDQNSSPVTKKVLFIETLPNMVQSKILSFL 84
Query: 65 VFPYIQFVMSE 75
+F Y +F +S+
Sbjct: 85 LFEYQRFCVSD 95
>At4g00360 probable cytochrome P450
Length = 553
Score = 31.6 bits (70), Expect = 0.34
Identities = 13/32 (40%), Positives = 18/32 (55%)
Query: 171 HHRELEAELKKMAPVNGRAVLVLRGKCGCSVG 202
H R+LE +K + P V+VL GKC +G
Sbjct: 509 HKRDLEVMMKSLVPKERNDVVVLNGKCNGGIG 540
>At1g62305 putative protein
Length = 354
Score = 30.4 bits (67), Expect = 0.75
Identities = 19/71 (26%), Positives = 32/71 (44%), Gaps = 2/71 (2%)
Query: 82 LFMDLVKEEVAVAPVIYVSLGVSVSCAVVATWFFIAYTSRKCSNPNCKGLK--NAAEFDI 139
L M ++ E+ V Y + +S A +W + +TS C +G+K N ++
Sbjct: 249 LTMRGLENEMERRTVTYTTWNLSAKKAEAKSWHPLTFTSDNCGPEEIEGIKKINHVYYES 308
Query: 140 QLETEDCVKNS 150
+ TE C NS
Sbjct: 309 EYRTEWCRANS 319
>At1g02530 hypothetical protein
Length = 1273
Score = 30.0 bits (66), Expect = 0.98
Identities = 20/103 (19%), Positives = 46/103 (44%)
Query: 2 VNFHSLISSVDQSSSSMANSIDLAKSRNRKTLNSLQIPQCERSRSAVVDVVIFIAVVIAL 61
+N L + +D S S + + +++ L + + + + V++ VV A+
Sbjct: 655 LNVLGLFAGLDLGSGSQRVGQEETGTTSQEPLRKVSLTRIAALNKPEIPVLLLGTVVAAI 714
Query: 62 GFLVFPYIQFVMSESFKLCGLFMDLVKEEVAVAPVIYVSLGVS 104
+FP ++S + D +K++ +I+V+LGV+
Sbjct: 715 NGAIFPLFGILISRVIEAFFKPADQLKKDSRFWAIIFVALGVT 757
>At2g01950 putative receptor protein kinase
Length = 1143
Score = 29.6 bits (65), Expect = 1.3
Identities = 17/53 (32%), Positives = 26/53 (48%), Gaps = 1/53 (1%)
Query: 155 KDGGGIK-KGLFKIPCDHHRELEAELKKMAPVNGRAVLVLRGKCGCSVGRLEV 206
KDG + K L ++ C RE AE++ + + R ++ L G C RL V
Sbjct: 858 KDGSSVAIKKLIRLSCQGDREFMAEMETLGKIKHRNLVPLLGYCKIGEERLLV 910
>At5g23660 MtN3-like protein
Length = 285
Score = 27.7 bits (60), Expect = 4.8
Identities = 25/93 (26%), Positives = 44/93 (46%), Gaps = 11/93 (11%)
Query: 53 IFIAVVIALGFLVFPYIQFVMSESFKLCGLFMDLVKEEVAVAPVIYVS-----LGVSVSC 107
I++A +GF V +Q ++ +K C DLV++E+ A + VS LG S
Sbjct: 191 IYVAFPNVIGF-VLGALQMILYVVYKYCKTPSDLVEKELEAAKLPEVSIDMVKLGTLTSP 249
Query: 108 AVVATWFFIAYTSRKCSNPNC-----KGLKNAA 135
VA + + C++ N +G++N+A
Sbjct: 250 EPVAITVVRSVNTCNCNDRNAEIENGQGVRNSA 282
>At5g41850 unknown protein
Length = 224
Score = 26.9 bits (58), Expect = 8.3
Identities = 24/86 (27%), Positives = 39/86 (44%), Gaps = 9/86 (10%)
Query: 66 FPYI----QFVMSESFKLCGLFMDLVKEEVAVAP-----VIYVSLGVSVSCAVVATWFFI 116
+PY+ + V ++ KL +++VKE A P ++ S+G VSC V A +
Sbjct: 58 YPYLADGKKRVAPKAEKLIEFHLNVVKETAAKFPGHPLILVGKSMGSRVSCMVSAVNEDV 117
Query: 117 AYTSRKCSNPNCKGLKNAAEFDIQLE 142
++ C KG K A + LE
Sbjct: 118 TVSAVICLGYPLKGAKGAIRDETLLE 143
>At1g08070 unknown protein
Length = 741
Score = 26.9 bits (58), Expect = 8.3
Identities = 16/35 (45%), Positives = 19/35 (53%)
Query: 4 FHSLISSVDQSSSSMANSIDLAKSRNRKTLNSLQI 38
FH L SS D S+ N L+ N KTL SL+I
Sbjct: 17 FHFLPSSSDPPYDSIRNHPSLSLLHNCKTLQSLRI 51
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.136 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,582,324
Number of Sequences: 26719
Number of extensions: 179563
Number of successful extensions: 448
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 431
Number of HSP's gapped (non-prelim): 12
length of query: 217
length of database: 11,318,596
effective HSP length: 95
effective length of query: 122
effective length of database: 8,780,291
effective search space: 1071195502
effective search space used: 1071195502
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC140025.11