BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139841.3 + phase: 0
(154 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55280 ribosomal L23a - like protein 259 4e-70
At2g39460 60S ribosomal protein L23A 256 4e-69
At4g02110 predicted protein of unknown function 32 0.18
At5g38720 unknown protein 28 1.5
At1g76950 zinc finger protein (PRAF1) 28 1.5
At1g05950 hypothetical protein 28 1.5
At5g23850 putative protein 27 3.3
At5g61450 Unknown protein (MCI2.1) 27 4.3
At4g39380 unknown protein 27 4.3
At4g11160 translation initiation factor IF-2 like protein 27 4.3
At1g60890 putative phosphatidylinositol-4-phosphate 5-kinase (At... 27 4.3
At1g06760 histone H1 like protein 27 4.3
At5g11010 unknown protein 27 5.6
At3g29450 hypothetical protein 27 5.6
At2g30620 putative histone H1 protein 27 5.6
At5g02300 ubiquitin-protein ligase E3-alpha -like protein 26 7.4
At2g28090 hypothetical protein 26 7.4
At5g51160 putative protein 26 9.6
At5g44740 translesion synthesis polymerase RAD30 like protein 26 9.6
At5g08550 putative protein 26 9.6
>At3g55280 ribosomal L23a - like protein
Length = 154
Score = 259 bits (662), Expect = 4e-70
Identities = 132/148 (89%), Positives = 138/148 (93%), Gaps = 1/148 (0%)
Query: 8 DVSKKTDPKAQALKAAKAVKSGTAIKK-AKKIRTTVTFHRPKTQTKDRNPKYPRISATPR 66
DV+KK DPKA+ALKAAKAVKSG +KK AKKIRT VTFHRPKT T R PKYP+ISATPR
Sbjct: 7 DVTKKADPKAKALKAAKAVKSGQIVKKPAKKIRTKVTFHRPKTLTVPRKPKYPKISATPR 66
Query: 67 NKLDHYQILKFPLTTESAMKKIEDNNTLVFIVDLRADKKKIKDAVKKMYDIQAKKVNTLI 126
NKLDHYQILK+PLTTESAMKKIEDNNTLVFIVD+RADKKKIKDAVKKMYDIQ KKVNTLI
Sbjct: 67 NKLDHYQILKYPLTTESAMKKIEDNNTLVFIVDIRADKKKIKDAVKKMYDIQTKKVNTLI 126
Query: 127 RPDGTKKAYVRLTPDYDALDVANKIGII 154
RPDGTKKAYVRLTPDYDALDVANKIGII
Sbjct: 127 RPDGTKKAYVRLTPDYDALDVANKIGII 154
>At2g39460 60S ribosomal protein L23A
Length = 154
Score = 256 bits (653), Expect = 4e-69
Identities = 131/148 (88%), Positives = 136/148 (91%), Gaps = 1/148 (0%)
Query: 8 DVSKKTDPKAQALKAAKAVKSGTAIKKA-KKIRTTVTFHRPKTQTKDRNPKYPRISATPR 66
D +KK DPKA+ALKAAKAVKSG A KK KKIRT VTFHRPKT TK R KYP+ISATPR
Sbjct: 7 DTTKKADPKAKALKAAKAVKSGQAFKKKDKKIRTKVTFHRPKTLTKPRTGKYPKISATPR 66
Query: 67 NKLDHYQILKFPLTTESAMKKIEDNNTLVFIVDLRADKKKIKDAVKKMYDIQAKKVNTLI 126
NKLDHYQILK+PLTTESAMKKIEDNNTLVFIVD+RADKKKIKDAVKKMYDIQ KKVNTLI
Sbjct: 67 NKLDHYQILKYPLTTESAMKKIEDNNTLVFIVDIRADKKKIKDAVKKMYDIQTKKVNTLI 126
Query: 127 RPDGTKKAYVRLTPDYDALDVANKIGII 154
RPDGTKKAYVRLTPDYDALDVANKIGII
Sbjct: 127 RPDGTKKAYVRLTPDYDALDVANKIGII 154
>At4g02110 predicted protein of unknown function
Length = 1293
Score = 31.6 bits (70), Expect = 0.18
Identities = 19/54 (35%), Positives = 32/54 (59%), Gaps = 2/54 (3%)
Query: 21 KAAKAVKSGT-AIKKAKKIRTTVTFHRPKTQTKDRNPKYPRISATPRNKLDHYQ 73
K AK+ K+GT A K++K++R R Q ++ PK+ +S PR++ + YQ
Sbjct: 1025 KVAKSAKTGTKAEKESKQLRVNPLASRKVFQDQEHEPKFFIVSG-PRSQRNEYQ 1077
>At5g38720 unknown protein
Length = 306
Score = 28.5 bits (62), Expect = 1.5
Identities = 30/126 (23%), Positives = 53/126 (41%), Gaps = 12/126 (9%)
Query: 8 DVSKKTDPKAQALKAAKAVKSGTA-IKKAKKIRTTVTFHRPKTQTKDRNPKY-------P 59
+V DP+A L+A + K I+K K+I+++ + + K R K P
Sbjct: 32 NVELSEDPQAAQLQAKSSEKPNRKKIQKGKEIKSSPADGKLSGKMKKRKEKVGNVDISEP 91
Query: 60 RISATPRNKLDHYQILKFPLTTESAMKKIEDNNTLVFIVDLRADKKKIKDAVKKMYDIQA 119
+ A K+ K ++ K+ + T + DL+ + K K KK D+ +
Sbjct: 92 ILEAISTEKVKE----KKGKMNKTKKKRKAEEITRSSVEDLKRESKFKKSNKKKKMDMTS 147
Query: 120 KKVNTL 125
KK N +
Sbjct: 148 KKENKI 153
>At1g76950 zinc finger protein (PRAF1)
Length = 1103
Score = 28.5 bits (62), Expect = 1.5
Identities = 21/90 (23%), Positives = 39/90 (43%), Gaps = 2/90 (2%)
Query: 2 HCFCAGDVSKKTDPKAQALKAAKAVKSGTAIKKAKKIRTTVTFHRPKTQTKDRNPKYPRI 61
+C+ G V + +A +AA A +G + ++ T RN PR+
Sbjct: 653 NCYNCGLVHCHSCSSKKAFRAALAPSAGRLYRVCDSCYVKLSKVSEINDTNRRNSAVPRL 712
Query: 62 SATPRNKLDHYQI--LKFPLTTESAMKKIE 89
S R++LD +I KF + +K+++
Sbjct: 713 SGENRDRLDKSEIRLAKFGTSNMDLIKQLD 742
>At1g05950 hypothetical protein
Length = 590
Score = 28.5 bits (62), Expect = 1.5
Identities = 16/40 (40%), Positives = 25/40 (62%), Gaps = 1/40 (2%)
Query: 104 KKKIKDAVKKMYDIQAKKVNTLIRPDGTK-KAYVRLTPDY 142
+++ K A+K+ Y+I+AKKV L+ G + KA RL Y
Sbjct: 287 ERREKAALKRRYEIKAKKVAALLSHPGARGKATTRLQNRY 326
>At5g23850 putative protein
Length = 542
Score = 27.3 bits (59), Expect = 3.3
Identities = 15/31 (48%), Positives = 17/31 (54%), Gaps = 3/31 (9%)
Query: 30 TAIKKAKKIRTTVTFHRPKTQTKDRNPKYPR 60
T + KK TT T KTQT+ PKYPR
Sbjct: 62 TTVLLEKKAATTTT---TKTQTQTITPKYPR 89
>At5g61450 Unknown protein (MCI2.1)
Length = 447
Score = 26.9 bits (58), Expect = 4.3
Identities = 19/66 (28%), Positives = 31/66 (46%), Gaps = 5/66 (7%)
Query: 48 KTQTKDRNPKYPRISATPRNKLDHYQILKFPLTTESAMKKIEDNNTLVFIVDLRADKKKI 107
K T D+NP+ S++ + D+ + P + E A+ +E NN + D +
Sbjct: 275 KDLTSDKNPE---ASSSNTKETDNSAVK--PHSHEEAVASVEANNLSEKVTQCTIDAESS 329
Query: 108 KDAVKK 113
KDA KK
Sbjct: 330 KDAEKK 335
>At4g39380 unknown protein
Length = 518
Score = 26.9 bits (58), Expect = 4.3
Identities = 24/94 (25%), Positives = 38/94 (39%), Gaps = 14/94 (14%)
Query: 11 KKTDPKAQALKAAKAVKSGTAIKKAKKIRTTVTFHRPKTQTKDRNPKYPRISATPRNKLD 70
KKT+ ++ K KA T KK+ K TT H+ + KY + SA P +
Sbjct: 4 KKTETATESSKEIKAKGKFTRAKKSMK-TTTKNIHQSGKHNSGKEEKY-KCSAQPSS--- 58
Query: 71 HYQILKFPLTTESAMKKIEDNNTLVFIVDLRADK 104
+ E + ED V ++D+ + K
Sbjct: 59 ---------SQEQRLIHTEDQTQKVPVLDIHSSK 83
>At4g11160 translation initiation factor IF-2 like protein
Length = 743
Score = 26.9 bits (58), Expect = 4.3
Identities = 19/62 (30%), Positives = 33/62 (52%), Gaps = 2/62 (3%)
Query: 75 LKFPLTTESAMKKIEDNNTLVFIVDLRADKKKIKDAVKKMYDIQAKKVNTLIRPDGTKKA 134
+K+ LT+E ++ED V V++ A K D +++ +QA ++ R DG +A
Sbjct: 340 VKYQLTSEGI--ELEDIGGNVQAVEVSAAKSTGLDKLEEALLLQAVDMDLKARVDGPAQA 397
Query: 135 YV 136
YV
Sbjct: 398 YV 399
>At1g60890 putative phosphatidylinositol-4-phosphate 5-kinase
(At1g60890)
Length = 769
Score = 26.9 bits (58), Expect = 4.3
Identities = 16/49 (32%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Query: 49 TQTKDRNPK-YPRISATPRNKLDHYQILKFPLTTESAMKKIEDNNTLVF 96
T+T +R P Y S PR+ + H K + +S +I D N +VF
Sbjct: 250 TRTSERYPDDYWSTSDPPRDFMHHGPSSKSARSVDSGQSEIRDKNPIVF 298
>At1g06760 histone H1 like protein
Length = 274
Score = 26.9 bits (58), Expect = 4.3
Identities = 23/66 (34%), Positives = 29/66 (43%), Gaps = 6/66 (9%)
Query: 9 VSKKTDPKAQALKAAKAVKSGTAIKKA----KKIRTTVTFHRP--KTQTKDRNPKYPRIS 62
V K KA+ KAAK K + KKA KK+ T T + K K + K P
Sbjct: 208 VDAKPKAKARPAKAAKTAKVTSPAKKAVAATKKVATVATKKKTPVKKVVKPKTVKSPAKR 267
Query: 63 ATPRNK 68
A+ R K
Sbjct: 268 ASSRVK 273
>At5g11010 unknown protein
Length = 368
Score = 26.6 bits (57), Expect = 5.6
Identities = 11/15 (73%), Positives = 11/15 (73%)
Query: 3 CFCAGDVSKKTDPKA 17
CF GDVS K DPKA
Sbjct: 103 CFFYGDVSSKRDPKA 117
>At3g29450 hypothetical protein
Length = 522
Score = 26.6 bits (57), Expect = 5.6
Identities = 14/48 (29%), Positives = 24/48 (49%), Gaps = 1/48 (2%)
Query: 55 NPKYPRISATPRNKLDHYQILKFPLTTESAMKKIEDNNTLVFIVDLRA 102
+P+ PR + N L HYQ+ F + +++ D NT +F D +
Sbjct: 453 DPRKPRKKSNMTN-LHHYQVECFNTVLDMQIQEFNDPNTSLFARDFNS 499
>At2g30620 putative histone H1 protein
Length = 273
Score = 26.6 bits (57), Expect = 5.6
Identities = 29/118 (24%), Positives = 44/118 (36%), Gaps = 6/118 (5%)
Query: 17 AQALKAAKAVKSGTAIKKAKKIRTTVTFHRPKTQTKDRNPKYPRISATPRNKLDHYQILK 76
A A AKA GT AK + +PK + PK ++A + K +
Sbjct: 160 AVAPAKAKAAAKGTKKPAAKVVAKAKVTAKPKAKVTAAKPKSKSVAAVSKTK----AVAA 215
Query: 77 FPLTTESAMKKIEDNNTLVFIVDLRADKKKIKDAVKKMYDIQAKKVNTLIRPDGTKKA 134
P E K + + A KK+ AV K ++ KV + + T+KA
Sbjct: 216 KPKAKERPAKASRTSTRTSPGKKVAAPAKKV--AVTKKAPAKSVKVKSPAKRASTRKA 271
>At5g02300 ubiquitin-protein ligase E3-alpha -like protein
Length = 1225
Score = 26.2 bits (56), Expect = 7.4
Identities = 18/71 (25%), Positives = 41/71 (57%), Gaps = 5/71 (7%)
Query: 74 ILKFPLTTESAM----KKIEDNNTLVFIVDLRADKKKIKDAVKKMYDIQAKKVNTLIRPD 129
+L+F +++ES + ++I ++ L+ I+ L+A++ +D +KK++D+ K + +
Sbjct: 254 LLEFSMSSESLLSFVSRRIISSSGLLSIL-LKAERFLDQDVMKKLHDLFLKLIGDPVFKC 312
Query: 130 GTKKAYVRLTP 140
KA+V P
Sbjct: 313 EFAKAFVSYYP 323
>At2g28090 hypothetical protein
Length = 245
Score = 26.2 bits (56), Expect = 7.4
Identities = 30/134 (22%), Positives = 56/134 (41%), Gaps = 11/134 (8%)
Query: 26 VKSGTAIKKAKKIRTTVTFHRPKTQTKDRNPKYPRISATPRNKLDHYQILKFPLTTESAM 85
VK ++K K + + +PK TK+ N K T +LK + + +
Sbjct: 78 VKIAEKLQKKSKKKVELISPKPKKDTKENNEKKAN-DKTQTVVAVTTVVLKVNCSCDGCI 136
Query: 86 KKIEDN-NTLVFIVDLRADKKKIKDAVKKMYDIQA------KKVNTLIRPDGTKKAYVRL 138
K+I+ +T + ++ DK+K V DI++ +K+ ++ KK +
Sbjct: 137 KRIQKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSVTDNLKRKLKKTVQVVPEKK---KK 193
Query: 139 TPDYDALDVANKIG 152
D D +V K+G
Sbjct: 194 KKDKDNAEVNTKVG 207
>At5g51160 putative protein
Length = 442
Score = 25.8 bits (55), Expect = 9.6
Identities = 13/43 (30%), Positives = 22/43 (50%)
Query: 28 SGTAIKKAKKIRTTVTFHRPKTQTKDRNPKYPRISATPRNKLD 70
+ A + + +R T+ + + KDR+ K P AT R K+D
Sbjct: 53 AAAAAGQVETVRATLGVEKKLCRLKDRDGKTPLHVATMRGKID 95
>At5g44740 translesion synthesis polymerase RAD30 like protein
Length = 672
Score = 25.8 bits (55), Expect = 9.6
Identities = 20/72 (27%), Positives = 34/72 (46%), Gaps = 15/72 (20%)
Query: 10 SKKTDPKAQALKAAKAVKS-------------GTAIKKAKKIRTTVTFHRPKTQTK--DR 54
S KT P +ALK+ V+ G+ +++ K+I +T+T H ++K D
Sbjct: 316 SGKTFPGPRALKSLSTVQHWLNQLSEELSERLGSDLEQNKRIASTLTLHASAFRSKDSDS 375
Query: 55 NPKYPRISATPR 66
+ K+P S R
Sbjct: 376 HKKFPSKSCPMR 387
>At5g08550 putative protein
Length = 908
Score = 25.8 bits (55), Expect = 9.6
Identities = 11/26 (42%), Positives = 18/26 (68%)
Query: 104 KKKIKDAVKKMYDIQAKKVNTLIRPD 129
KK +KD VKK+ + AK +++L + D
Sbjct: 360 KKALKDNVKKLKESHAKTLSSLTKTD 385
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.132 0.370
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,168,521
Number of Sequences: 26719
Number of extensions: 114528
Number of successful extensions: 385
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 375
Number of HSP's gapped (non-prelim): 23
length of query: 154
length of database: 11,318,596
effective HSP length: 91
effective length of query: 63
effective length of database: 8,887,167
effective search space: 559891521
effective search space used: 559891521
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC139841.3