BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139708.3 + phase: 0
(150 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g61910 unknown protein 142 5e-35
At2g32910 unknown protein 130 2e-31
At2g35140 hypothetical protein 96 9e-21
At3g11000 unknown protein 81 2e-16
At5g42050 unknown protein 76 6e-15
At3g27090 gda-1, putative 69 1e-12
At5g01660 putative protein 64 2e-11
At1g78000 sulfate transporter (Sultr1;2) 28 1.9
At4g26540 receptor protein kinase like protein 27 3.2
At2g45790 putative phosphomannomutase 27 3.2
At4g25730 unknown protein 27 4.1
At3g56970 putative bHLH transcription factor (bHLH038) 27 4.1
At5g54480 unknown protein 26 7.0
At5g07180 receptor-like protein kinase 26 7.0
At1g74360 putative receptor protein kinase (At1g74360) 26 7.0
At1g71810 unknown protein 26 7.0
At1g71250 putative GDSL-motif lipase/acylhydrolase 26 7.0
At5g42430 putative protein 26 9.2
At4g32700 putative protein 26 9.2
At1g35710 protein kinase, putative 26 9.2
>At5g61910 unknown protein
Length = 738
Score = 142 bits (359), Expect = 5e-35
Identities = 71/130 (54%), Positives = 91/130 (69%), Gaps = 1/130 (0%)
Query: 9 DTPDKLAGFIFMCNRMTKPECFSYRVFALPAGRKHIVERINPGMHLFLFDTDVKLLYGIY 68
D ++L G+IFMCN TK +C+ YRVF +P G K +VE I PGM LFL+D + +LLYG+Y
Sbjct: 56 DHHEQLPGYIFMCNGRTKTDCYRYRVFGIPRGGKDVVESIKPGMKLFLYDFEKRLLYGVY 115
Query: 69 LATSTGMLNIEPYAFGGRFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHYKFNPELNIR 128
AT G L+IEP AF G++PAQV F+I+ +CLPL EN FK AI +NY K KF EL+
Sbjct: 116 EATVGGRLDIEPEAFEGKYPAQVGFRIVMNCLPLTENTFKSAIYENY-KGSKFKQELSPH 174
Query: 129 QVSSAIVTFR 138
QV S + FR
Sbjct: 175 QVMSLLSLFR 184
>At2g32910 unknown protein
Length = 691
Score = 130 bits (328), Expect = 2e-31
Identities = 63/126 (50%), Positives = 81/126 (64%)
Query: 13 KLAGFIFMCNRMTKPECFSYRVFALPAGRKHIVERINPGMHLFLFDTDVKLLYGIYLATS 72
K+ G IFMCN T+P+CF + V + RK V+ I PG+ LFL+D D+KLLYGI+ A+S
Sbjct: 318 KIGGLIFMCNTKTRPDCFRFSVMGVQEKRKDFVKGIKPGLKLFLYDYDLKLLYGIFEASS 377
Query: 73 TGMLNIEPYAFGGRFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHYKFNPELNIRQVSS 132
G + +E AFGG FPAQV FK+ DC+PL E+ FK AI +NY KF EL +QV
Sbjct: 378 AGGMKLERNAFGGSFPAQVRFKVFSDCIPLAESQFKKAIIENYNNKNKFKTELTHKQVFK 437
Query: 133 AIVTFR 138
FR
Sbjct: 438 LKKLFR 443
>At2g35140 hypothetical protein
Length = 879
Score = 95.5 bits (236), Expect = 9e-21
Identities = 54/125 (43%), Positives = 73/125 (58%), Gaps = 3/125 (2%)
Query: 16 GFIFMCNRMTKPECFSYRVFALPAGRKHIVERINPGMHLFLFDTDVKLLYGIYLATSTGM 75
G IFM N T+ EC S ++F LP G V+ + GM LFLF+ + + L+G++ A S G
Sbjct: 23 GAIFMSNNSTRKECLSRKLFGLPIGLGGFVKHVKAGMMLFLFEFEKRELHGVFQACSDGA 82
Query: 76 LNIEPYAF---GGRFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHYKFNPELNIRQVSS 132
+NIEP AF G +FPAQV F C PL E++F +AI +NY KFN L+ QV
Sbjct: 83 INIEPNAFRSSGKQFPAQVKFTEKWRCRPLCESEFGNAIHENYFTPTKFNFGLSKAQVQR 142
Query: 133 AIVTF 137
+ F
Sbjct: 143 LLKLF 147
>At3g11000 unknown protein
Length = 488
Score = 81.3 bits (199), Expect = 2e-16
Identities = 47/131 (35%), Positives = 68/131 (51%), Gaps = 6/131 (4%)
Query: 14 LAGFIFMCNRMTKPECFSYRVFALPAGRKHIVERINPGMHLFLFDTDVKLLYGIYLATST 73
L G IF C T EC++ +F LPA ++ I+PG+ LFLF+ + L+GI+ A S
Sbjct: 11 LCGVIFGCKFSTIKECYAKNLFGLPAPHMAYIKNIDPGLTLFLFNYSDRTLHGIFEAASE 70
Query: 74 GMLNIEPYAFG------GRFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHYKFNPELNI 127
G LNI+ A+ +PAQV ++ C PLPE F I +NY F EL+
Sbjct: 71 GKLNIDSKAWSPNGTDPSPYPAQVKVRVRVRCEPLPEEKFSPVIVENYNDDKMFWFELDR 130
Query: 128 RQVSSAIVTFR 138
Q + + F+
Sbjct: 131 GQTNKLLRLFK 141
>At5g42050 unknown protein
Length = 349
Score = 76.3 bits (186), Expect = 6e-15
Identities = 51/136 (37%), Positives = 69/136 (50%), Gaps = 15/136 (11%)
Query: 12 DKLAGFIFMCNRMTKPECFSYRVFALPAGRKHIVERINPGMHLFLFDTDVKLLYGIYLAT 71
+ + G+IF+CN T E ++F LP + V I PG+ LFL++ L+GIY A
Sbjct: 214 ETIGGYIFVCNNDTMEENLKRQLFGLPPRYRDSVRAITPGLPLFLYNYSTHQLHGIYEAA 273
Query: 72 STGMLNIEPYAF-------GGRFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHY---KF 121
S G NIE AF RFPAQV K CLPL E+ F+ + HY KF
Sbjct: 274 SFGGTNIELNAFEDKKCPGESRFPAQVRAITRKVCLPLEEDSFRPIL-----HHYDGPKF 328
Query: 122 NPELNIRQVSSAIVTF 137
EL++ +V S + F
Sbjct: 329 RLELSVPEVLSLLDIF 344
>At3g27090 gda-1, putative
Length = 296
Score = 68.6 bits (166), Expect = 1e-12
Identities = 44/124 (35%), Positives = 63/124 (50%), Gaps = 15/124 (12%)
Query: 14 LAGFIFMCNRMTKPECFSYRVFALPAGRKHIVERINPGMHLFLFDTDVKLLYGIYLATST 73
L G+IF+CN T E +F LP + V I PG+ LFL++ L+GI+ AT+
Sbjct: 162 LGGYIFVCNNDTMQEDMKRHLFGLPPRYRDSVRAITPGLPLFLYNYTTHQLHGIFEATTF 221
Query: 74 GMLNIEPYAF-------GGRFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHY---KFNP 123
G NI+ A+ RFPAQV ++ K C L E+ F+ + HY KF
Sbjct: 222 GGTNIDATAWEDKKCKGESRFPAQVRIRVRKICKALEEDSFRPVL-----HHYDGPKFRL 276
Query: 124 ELNI 127
EL++
Sbjct: 277 ELSV 280
>At5g01660 putative protein
Length = 621
Score = 64.3 bits (155), Expect = 2e-11
Identities = 36/96 (37%), Positives = 51/96 (52%), Gaps = 6/96 (6%)
Query: 37 LPAGRKHIVERINPGMHLFLFDTDVKLLYGIYLATSTGMLNIEPYAFGG------RFPAQ 90
LP+ V++I+ G+ LFLF+ + L+GI+ A G LN +PY + +PAQ
Sbjct: 6 LPSNHYPYVQKIDIGLPLFLFNYSDRTLHGIFEAAGCGQLNFDPYGWTSDGSERTSYPAQ 65
Query: 91 VSFKILKDCLPLPENDFKHAIKDNYQKHYKFNPELN 126
V + C PL E FK AI DNY + F EL+
Sbjct: 66 VPISVRLQCEPLSEEKFKPAIADNYYSSHHFWFELD 101
>At1g78000 sulfate transporter (Sultr1;2)
Length = 653
Score = 28.1 bits (61), Expect = 1.9
Identities = 17/55 (30%), Positives = 26/55 (46%), Gaps = 1/55 (1%)
Query: 42 KHIVERINPGMHLFLFDTDVKLLYGIYLATSTGMLNI-EPYAFGGRFPAQVSFKI 95
KH+ + INP ++ T L GI + GM+ + E A G F A ++I
Sbjct: 313 KHLDQGINPSSFHLIYFTGDNLAKGIRIGVVAGMVALTEAVAIGRTFAAMKDYQI 367
>At4g26540 receptor protein kinase like protein
Length = 926
Score = 27.3 bits (59), Expect = 3.2
Identities = 17/48 (35%), Positives = 20/48 (41%)
Query: 66 GIYLATSTGMLNIEPYAFGGRFPAQVSFKILKDCLPLPENDFKHAIKD 113
GI L T LN+ G P ++S L L ENDF I D
Sbjct: 519 GIGLLTELTKLNLAKNRLSGEIPREISTCRSLQLLNLGENDFSGEIPD 566
>At2g45790 putative phosphomannomutase
Length = 246
Score = 27.3 bits (59), Expect = 3.2
Identities = 14/44 (31%), Positives = 23/44 (51%), Gaps = 1/44 (2%)
Query: 37 LPAGRKHIVERINPGMHLFLFDTDVKLLYGIYLATSTGMLNIEP 80
L G + E IN +H ++ D D+ + G ++ GMLN+ P
Sbjct: 90 LHLGDDKLKELINFTLH-YIADLDIPIKRGTFIEFRNGMLNVSP 132
>At4g25730 unknown protein
Length = 821
Score = 26.9 bits (58), Expect = 4.1
Identities = 16/32 (50%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Query: 94 KILKDCLP-LPENDFKHAIKDNYQKHYKFNPE 124
KIL D LP L +NDFKH +K Q PE
Sbjct: 297 KILCDDLPVLGKNDFKHILKWRMQIRKALTPE 328
>At3g56970 putative bHLH transcription factor (bHLH038)
Length = 253
Score = 26.9 bits (58), Expect = 4.1
Identities = 12/33 (36%), Positives = 17/33 (51%)
Query: 93 FKILKDCLPLPENDFKHAIKDNYQKHYKFNPEL 125
F L+ CLP + K +I + K K+ PEL
Sbjct: 91 FSSLRSCLPASDQSKKLSIPETVSKSLKYIPEL 123
>At5g54480 unknown protein
Length = 720
Score = 26.2 bits (56), Expect = 7.0
Identities = 11/30 (36%), Positives = 19/30 (62%)
Query: 103 PENDFKHAIKDNYQKHYKFNPELNIRQVSS 132
PE+D+ I+ N +K+ K + N+R+ SS
Sbjct: 212 PESDYNSLIRKNKKKNKKKKKKKNVRESSS 241
>At5g07180 receptor-like protein kinase
Length = 932
Score = 26.2 bits (56), Expect = 7.0
Identities = 13/36 (36%), Positives = 20/36 (55%)
Query: 76 LNIEPYAFGGRFPAQVSFKILKDCLPLPENDFKHAI 111
LN+ +F G+ PA++ I D L L N+F +I
Sbjct: 378 LNLSSNSFKGKIPAELGHIINLDTLDLSGNNFSGSI 413
>At1g74360 putative receptor protein kinase (At1g74360)
Length = 1106
Score = 26.2 bits (56), Expect = 7.0
Identities = 11/18 (61%), Positives = 13/18 (72%)
Query: 75 MLNIEPYAFGGRFPAQVS 92
ML++ AFGG FP QVS
Sbjct: 256 MLDLSGNAFGGEFPGQVS 273
>At1g71810 unknown protein
Length = 692
Score = 26.2 bits (56), Expect = 7.0
Identities = 15/48 (31%), Positives = 21/48 (43%)
Query: 66 GIYLATSTGMLNIEPYAFGGRFPAQVSFKILKDCLPLPENDFKHAIKD 113
G +L T G L + G F ++ ++ CL L DFK KD
Sbjct: 347 GNFLRTYDGQLAYLDFGMMGDFRPELRDGFMEACLHLVNRDFKALAKD 394
>At1g71250 putative GDSL-motif lipase/acylhydrolase
Length = 374
Score = 26.2 bits (56), Expect = 7.0
Identities = 10/27 (37%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
Query: 62 KLLYGI-YLATSTGMLNIEPYAFGGRF 87
++L G+ Y + + G+L++ Y +GGRF
Sbjct: 112 RILQGVNYASAAAGILDVSGYNYGGRF 138
>At5g42430 putative protein
Length = 395
Score = 25.8 bits (55), Expect = 9.2
Identities = 12/32 (37%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Query: 67 IYLATSTGMLNIEPYAFGGRFPAQVSFKILKD 98
IY G++N++ YA GG FP ++ +L+D
Sbjct: 251 IYYKGKLGVVNLK-YAHGGGFPLKLCMWVLED 281
>At4g32700 putative protein
Length = 1548
Score = 25.8 bits (55), Expect = 9.2
Identities = 19/56 (33%), Positives = 28/56 (49%), Gaps = 8/56 (14%)
Query: 89 AQVSFKILKDCLPLPENDFKHAIKDNYQKHYKFNPELNIRQVSSAIVTFRF-CFCF 143
A V F LK LP+PEN K + + KH L++ + S ++ F + FCF
Sbjct: 1185 ANVLFGQLK--LPIPENQSKGKLHPSTDKHC-----LDLLRYSEWLIAFPYIAFCF 1233
>At1g35710 protein kinase, putative
Length = 1120
Score = 25.8 bits (55), Expect = 9.2
Identities = 14/52 (26%), Positives = 21/52 (39%)
Query: 71 TSTGMLNIEPYAFGGRFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHYKFN 122
T+ L + GR PA +SF + L L N+F I + K +
Sbjct: 606 TNLSRLRLNGNQLSGRVPAGLSFLTNLESLDLSSNNFSSEIPQTFDSFLKLH 657
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.331 0.146 0.464
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,489,354
Number of Sequences: 26719
Number of extensions: 142866
Number of successful extensions: 338
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 318
Number of HSP's gapped (non-prelim): 21
length of query: 150
length of database: 11,318,596
effective HSP length: 90
effective length of query: 60
effective length of database: 8,913,886
effective search space: 534833160
effective search space used: 534833160
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC139708.3