BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138199.2 + phase: 0
(617 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g18270 unknown protein (At1g18270) 893 0.0
At1g14790 RNA-directed RNA polymerase, putative 38 0.019
At1g45160 similar to IRE homolog 1 dbj|BAA89784.1 33 0.47
At5g20330 beta-1,3-glucanase bg4 32 0.80
At5g51030 carbonyl reductase-like protein 32 1.0
At5g20340 beta-1,3-glucanase bg5 32 1.4
At5g59310 nonspecific lipid-transfer protein precursor - like 31 1.8
At1g30520 unknown protein 30 3.0
At1g08590 putative receptor kinase, CLV1 30 3.0
At5g43280 enoyl CoA hydratase-like protein 30 4.0
At2g36370 hypothetical protein 30 5.2
At2g17910 putative non-LTR retroelement reverse transcriptase 30 5.2
At1g49340 hypothetical protein 30 5.2
At4g18905 unknown protein 29 6.8
At4g05020 unknown protein 29 6.8
At4g02310 hypothetical protein 29 6.8
At3g45090 unknown protein 29 6.8
At1g33220 beta-1,3-glucanase, putative 29 6.8
At3g05830 unknown protein 29 8.8
At1g70570 unknown protein 29 8.8
>At1g18270 unknown protein (At1g18270)
Length = 1373
Score = 893 bits (2308), Expect = 0.0
Identities = 455/618 (73%), Positives = 537/618 (86%), Gaps = 3/618 (0%)
Query: 1 MDAWIICPFFLQGGRYTINDTHFVADSEMLVPAGDTEFAKDASFGYKSSNLCDWVEEKTN 60
MDAWIICPFFLQGGRYTI+D H+VADS+ LVPAG+TEFAKDASFGYKSSNL +WVEEKT
Sbjct: 758 MDAWIICPFFLQGGRYTIDDVHYVADSDRLVPAGETEFAKDASFGYKSSNLREWVEEKTA 817
Query: 61 GRILASSVVSISIQLLRKGGPDAVCKHLCSLKKGSVCIVNAASERDMTVFALGMIKAELS 120
G I A+SV SISIQLLRKGGPDAVC+ LCSLKKGS CIVNAASERDM VFA GMI+AEL
Sbjct: 818 GVIPANSVQSISIQLLRKGGPDAVCEFLCSLKKGSTCIVNAASERDMAVFAAGMIQAELK 877
Query: 121 GKRFLCRTAASFVSACIGIISKPPVLPKDLGIGRERNGGLTIVGSYVPKTTKQVEQLKLQ 180
G+ FLCRTAASFVSA IGII K PVLPKD +E +G L +VGSYVPKTTKQVE+L+ Q
Sbjct: 878 GRSFLCRTAASFVSALIGIIPKDPVLPKDFESNKESSGALIVVGSYVPKTTKQVEELQSQ 937
Query: 181 CGHFLRSIEASVEKLAMGSIEEREDEISRAAELADVYLKNRKDTLVMTSRNLITGKSASE 240
LRSIE SVEK+A+ S E R++EI RA E+AD +L+ ++TL+M+SR LITGK++SE
Sbjct: 938 HNQNLRSIEISVEKVALKSSEVRDEEIRRAVEMADAFLRAGRETLIMSSRELITGKTSSE 997
Query: 241 SLDINYKVSSALVEIMKRITTKPRYIIAKGGITSSDLATKALGAKCAKVVGQALAGIPLW 300
SLDIN KVSSALVE++ +I+T+PRYI+AKGGITSSD ATKAL A+ A V+GQALAG+P+W
Sbjct: 998 SLDINSKVSSALVEVVSQISTRPRYILAKGGITSSDTATKALKARRALVIGQALAGVPVW 1057
Query: 301 QLGPESRHPGVPYIVFPGNVGDSGALAQVVKSWTCPSTISSTKEILNNAEKGGYAVGAFN 360
+LGPESRHPGVPYIVFPGNVG+S ALA+VVKSW+ + STKE+L NAEKGGYAVGAFN
Sbjct: 1058 KLGPESRHPGVPYIVFPGNVGNSTALAEVVKSWSVVAG-RSTKELLLNAEKGGYAVGAFN 1116
Query: 361 VYNLEGVDAVVSAAEETQSPAILQIHPGALKQGGIPLVSCCISAAERASVPITVHFDHGT 420
VYNLEG++AVV+AAEE SPAILQ+HPGA KQGGIPLVSCCISAAE+A VPI+VHFDHGT
Sbjct: 1117 VYNLEGIEAVVAAAEEENSPAILQVHPGAFKQGGIPLVSCCISAAEQARVPISVHFDHGT 1176
Query: 421 SKEDLVEALEQGVSSLMVDGSHLSFEENVKYTKFISLLAHSKDMLVEAELGRLSGTEDDL 480
+K +L+EALE G+ S+MVDGSHLSF EN+ YTK I+ LA SK+++VEAELGRLSGTED L
Sbjct: 1177 TKHELLEALELGLDSVMVDGSHLSFTENLSYTKSITELARSKNIMVEAELGRLSGTEDGL 1236
Query: 481 TVEEYEAKLTDINKAEKFIDETGIDALAVCIGNVHGKYPASGPKLRLDLLKELRAVSQKK 540
TVE+YEAKLT++N+A++F+ ETGIDALAVCIGNVHGKYP SGP L+LDLLKEL A+S KK
Sbjct: 1237 TVEDYEAKLTNVNQAQEFM-ETGIDALAVCIGNVHGKYPKSGPNLKLDLLKELHALSSKK 1295
Query: 541 GVLLVLHGASGLGTELVKECINLGVRKFNVNTEVRKAYMDSLNT-PKSDLVNVMASARDA 599
GV LVLHGASGL L+KECI GVRKFNVNTEVR AYM++L++ K+D+V+VM++ + A
Sbjct: 1296 GVFLVLHGASGLSENLIKECIENGVRKFNVNTEVRTAYMEALSSGKKTDIVDVMSATKAA 1355
Query: 600 MKAVVAEKMHLFGSAGKA 617
MKAV+A+K+ LFGSAGKA
Sbjct: 1356 MKAVIADKIRLFGSAGKA 1373
>At1g14790 RNA-directed RNA polymerase, putative
Length = 1107
Score = 37.7 bits (86), Expect = 0.019
Identities = 17/72 (23%), Positives = 40/72 (54%)
Query: 35 DTEFAKDASFGYKSSNLCDWVEEKTNGRILASSVVSISIQLLRKGGPDAVCKHLCSLKKG 94
D F + A++ +K NL D+ KT IL+ ++ +S ++ +++ + + +L+K
Sbjct: 967 DEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTKRRDAESIGRAVRALRKE 1026
Query: 95 SVCIVNAASERD 106
++ + NA+ E +
Sbjct: 1027 TLSLFNASEEEE 1038
>At1g45160 similar to IRE homolog 1 dbj|BAA89784.1
Length = 1067
Score = 33.1 bits (74), Expect = 0.47
Identities = 15/44 (34%), Positives = 27/44 (61%)
Query: 178 KLQCGHFLRSIEASVEKLAMGSIEEREDEISRAAELADVYLKNR 221
KL C +L + E + +K ++G+I+E ED + A+ + LK+R
Sbjct: 621 KLLCEKYLHARELTADKSSVGNIKESEDVLEHASATPQLLLKDR 664
>At5g20330 beta-1,3-glucanase bg4
Length = 345
Score = 32.3 bits (72), Expect = 0.80
Identities = 32/122 (26%), Positives = 49/122 (39%), Gaps = 7/122 (5%)
Query: 271 GITSSDLATKALGAKCAKVVGQALAGIPLWQLGPESRHPGVPYIVFPGNVGDS-----GA 325
G+ DLA AL A V G A I + V V PG +G +
Sbjct: 87 GVKDQDLA--ALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQS 144
Query: 326 LAQVVKSWTCPSTISSTKEILNNAEKGGYAVGAFNVYNLEGVDAVVSAAEETQSPAILQI 385
L +VKS P +IS+ + N + + G F E + V+ +T +P ++ I
Sbjct: 145 LTNLVKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNI 204
Query: 386 HP 387
+P
Sbjct: 205 YP 206
>At5g51030 carbonyl reductase-like protein
Length = 314
Score = 32.0 bits (71), Expect = 1.0
Identities = 24/75 (32%), Positives = 37/75 (49%), Gaps = 10/75 (13%)
Query: 438 VDGSHLSFEENVKYTK-----FISLLAHS----KDMLVEAELGRLSGTEDDLTVEEYEAK 488
V+ SH+ N TK I L+ H+ + + V + LGRL G L E+ AK
Sbjct: 134 VEFSHMVISTNYYGTKNIINAMIPLMRHACQGARIVNVTSRLGRLKGRHSKLENEDVRAK 193
Query: 489 LTDINK-AEKFIDET 502
L D++ E+ +D+T
Sbjct: 194 LMDVDSLTEEIVDKT 208
>At5g20340 beta-1,3-glucanase bg5
Length = 354
Score = 31.6 bits (70), Expect = 1.4
Identities = 34/129 (26%), Positives = 51/129 (39%), Gaps = 7/129 (5%)
Query: 264 RYIIAKGGITSSDLATKALGAKCAKVVGQALAGIPLWQLGPESRHPGVPYIVFPGNVGDS 323
R I G+ DLA AL A V G I + V V PG +G
Sbjct: 89 RDIAVTVGVRDQDLA--ALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQ 146
Query: 324 -----GALAQVVKSWTCPSTISSTKEILNNAEKGGYAVGAFNVYNLEGVDAVVSAAEETQ 378
+L +VKS P +IS+ + N + + G F E + V+ +T
Sbjct: 147 VLPVMQSLTNLVKSRNLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTN 206
Query: 379 SPAILQIHP 387
SP +++I+P
Sbjct: 207 SPILVKIYP 215
>At5g59310 nonspecific lipid-transfer protein precursor - like
Length = 112
Score = 31.2 bits (69), Expect = 1.8
Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 5/54 (9%)
Query: 107 MTVFALGMIKAELSGKRFLCRTAASFVSACIGIISKPPVLPKDLGIGRERNGGL 160
+TVF + + A ++ C T AS +S C+G +SK V+P G ++ G+
Sbjct: 13 LTVFIVASVDAAIT-----CGTVASSLSPCLGYLSKGGVVPPPCCAGVKKLNGM 61
>At1g30520 unknown protein
Length = 560
Score = 30.4 bits (67), Expect = 3.0
Identities = 14/44 (31%), Positives = 26/44 (58%), Gaps = 3/44 (6%)
Query: 523 PKLRLDLLKELRAV---SQKKGVLLVLHGASGLGTELVKECINL 563
P + DL++ R ++ +GV +L+G L +EL+KE +N+
Sbjct: 268 PAMMADLIRVNRTTKNGAENRGVRKILNGGGSLSSELLKEAVNI 311
>At1g08590 putative receptor kinase, CLV1
Length = 1029
Score = 30.4 bits (67), Expect = 3.0
Identities = 29/98 (29%), Positives = 47/98 (47%), Gaps = 9/98 (9%)
Query: 439 DGSHLSFEENVKYTKFISLLAHSKDMLVEAELGRLSGTEDDLTVEEYEAKLTDINKAEKF 498
+GS S +N+K KF+ L ++ V +G LS E + Y + +I E+F
Sbjct: 186 EGSVPSSFKNLKNLKFLGLSGNNFGGKVPKVIGELSSLE--TIILGYNGFMGEI--PEEF 241
Query: 499 IDETGIDALAVCIGNVHGKYPASGPKLRLDLLKELRAV 536
T + L + +GN+ G+ P+S L LK+L V
Sbjct: 242 GKLTRLQYLDLAVGNLTGQIPSS-----LGQLKQLTTV 274
>At5g43280 enoyl CoA hydratase-like protein
Length = 278
Score = 30.0 bits (66), Expect = 4.0
Identities = 13/38 (34%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
Query: 364 LEGVDAVVSAAEETQSPAILQIHPGALKQGGIPLVSCC 401
++ + A ++A E+ + P I IH GA GG+ L++ C
Sbjct: 100 IKSMQAAITAIEQCRKPVIAAIH-GACIGGGVDLITAC 136
>At2g36370 hypothetical protein
Length = 593
Score = 29.6 bits (65), Expect = 5.2
Identities = 33/134 (24%), Positives = 53/134 (38%), Gaps = 19/134 (14%)
Query: 442 HLSFEENVKYTKFISLLAHSKDM--------------LVEAELGRLSGTEDDLTVEEYEA 487
H+S E + T ++L+ HS+ M L E L + T E ++
Sbjct: 385 HMSKCEGISETSLLNLITHSQKMKSLCLRDTKVSDSVLCEFPGSTLEALDISNTTEISDS 444
Query: 488 KLTDINKAEKFIDETGIDALAVCIGNVH-GKYPASGPKLRLDLLKELRAVSQKKGVLLVL 546
LT + + K + E AL+ C G + + S P LR L+ + +L++
Sbjct: 445 ALTSVLTSLKHLQEL---ALSYCFGEISLQSFKFSMPNLRKLRLERVTRWMTNDDLLVLT 501
Query: 547 HGASGLGTELVKEC 560
L TEL EC
Sbjct: 502 QSCPNL-TELSLEC 514
>At2g17910 putative non-LTR retroelement reverse transcriptase
Length = 1344
Score = 29.6 bits (65), Expect = 5.2
Identities = 14/48 (29%), Positives = 26/48 (54%)
Query: 426 VEALEQGVSSLMVDGSHLSFEENVKYTKFISLLAHSKDMLVEAELGRL 473
+E + + +S M G+H SF ++ + IS+ HS D ++ + RL
Sbjct: 231 IEVIHRSWNSAMSQGTHSSFFSLIECRRAISVWKHSSDTNAQSRIKRL 278
>At1g49340 hypothetical protein
Length = 1957
Score = 29.6 bits (65), Expect = 5.2
Identities = 20/55 (36%), Positives = 27/55 (48%), Gaps = 1/55 (1%)
Query: 311 VPYIVFPGNVGDSGALAQVVKSWTCPSTISSTKEILNNAEKGGYAVGAFNVYNLE 365
+PY V P NV ++ L Q + W S I+ E L A KG V A+ + LE
Sbjct: 1461 LPYFVTPKNVEENSVLLQQLPHWAACS-ITQALEFLTPAYKGHPRVMAYVLRVLE 1514
>At4g18905 unknown protein
Length = 494
Score = 29.3 bits (64), Expect = 6.8
Identities = 27/105 (25%), Positives = 53/105 (49%), Gaps = 8/105 (7%)
Query: 421 SKEDLVEALEQGVSSLMVDGSHLSFEENVKYT-KFISLLAHSKDMLVEAELGRLS---GT 476
SKE+L E +E G + V+GS+ EE ++ + IS + H+K V LG+ S
Sbjct: 24 SKEELKELIESGAFARNVEGSNEDEEEEIEEDGEEISEVDHAK--AVAEALGKSSKSKAV 81
Query: 477 EDDLTVEEYEAKLTDINKAEKFIDETGIDALAVCIGNVHGKYPAS 521
+ V+E L +++ ++ I+ + +G+++ YP++
Sbjct: 82 SSSMEVDEVSQGLKELDMDNYDEEDDEIELFSSGMGDLY--YPSN 124
>At4g05020 unknown protein
Length = 582
Score = 29.3 bits (64), Expect = 6.8
Identities = 13/40 (32%), Positives = 24/40 (59%)
Query: 463 DMLVEAELGRLSGTEDDLTVEEYEAKLTDINKAEKFIDET 502
D+L EAE +S +L +EE+++ L+ ++ KF+ T
Sbjct: 432 DLLKEAETDDVSKNNIELKIEEFKSALSQVDSQVKFLPAT 471
>At4g02310 hypothetical protein
Length = 349
Score = 29.3 bits (64), Expect = 6.8
Identities = 36/139 (25%), Positives = 57/139 (40%), Gaps = 15/139 (10%)
Query: 376 ETQSPAILQIHPGALKQGGIPLVSCCISAAERASVPITVHFDHGTSKEDLVEALEQGVSS 435
+++S IL G L+QG PLV+C +A + I V + E + V
Sbjct: 119 KSRSMWILDSRSGKLRQGPRPLVACDQAAVGLVNDKIYVFGGIDDMNKRYYEGIHAQVFD 178
Query: 436 LMVDGSHLSFEENVKYTKFISLLAHSKDMLVEAELGR---LSGTEDDLTVEEYEAKLTDI 492
L H+ +VK LA +V LGR + GT+ D+T+ Y+ K
Sbjct: 179 LKTQTWHVGPNLSVK-------LACLNRSVVTPSLGRKIYVRGTDRDVTI--YDIK---D 226
Query: 493 NKAEKFIDETGIDALAVCI 511
K +K I + +C+
Sbjct: 227 GKCDKIIPADDFSSGDMCV 245
>At3g45090 unknown protein
Length = 717
Score = 29.3 bits (64), Expect = 6.8
Identities = 23/71 (32%), Positives = 33/71 (46%), Gaps = 9/71 (12%)
Query: 483 EEYEAKLTDINKAEKFIDETGIDALAVCIGNVHGKYPASGPKLRLDLLKELRAVSQKKGV 542
E Y+ + T + E F+D DALA +Y G R DL+ R +K+ V
Sbjct: 109 EMYKRRTTVVVSREIFVDVV-CDALA--------EYKYVGRDQRADLILACRIRERKESV 159
Query: 543 LLVLHGASGLG 553
++L G SG G
Sbjct: 160 TVLLCGTSGCG 170
>At1g33220 beta-1,3-glucanase, putative
Length = 335
Score = 29.3 bits (64), Expect = 6.8
Identities = 20/78 (25%), Positives = 35/78 (44%), Gaps = 5/78 (6%)
Query: 315 VFPGNVGDS-----GALAQVVKSWTCPSTISSTKEILNNAEKGGYAVGAFNVYNLEGVDA 369
V PG +G +L +VKS P +IS+ + N + + G F E +
Sbjct: 129 VIPGPIGPQVLPVMQSLTILVKSMNLPISISTVVAMSNLEQSYPPSAGEFTSQAREQLVP 188
Query: 370 VVSAAEETQSPAILQIHP 387
V+ +T +P ++ I+P
Sbjct: 189 VLKLLSQTSTPILVNIYP 206
>At3g05830 unknown protein
Length = 336
Score = 28.9 bits (63), Expect = 8.8
Identities = 29/127 (22%), Positives = 53/127 (40%), Gaps = 15/127 (11%)
Query: 155 ERNGGLTIVGSYVPKTTKQVEQLKLQCGHFLRSIEASVE-----KLAMGSIEEREDEISR 209
ERN L S K K++E+ +L+ ++ EAS + K+ ++++ D+ +R
Sbjct: 78 ERNCQLEASASAADKFIKELEEFRLKLDTTKQTAEASADSAQSTKIQCSMLKQQLDDKTR 137
Query: 210 AAELADVYLKNRKDTLVMTSRNLITGKSASESLDINYKVSSALVEIMKRITTKPRYIIAK 269
+ L+ ++D + L L + L E ++RI + IAK
Sbjct: 138 S-------LREQEDRMTQLGHQL---DDLQRGLSLRECSEKQLREEVRRIEREVTEAIAK 187
Query: 270 GGITSSD 276
GI D
Sbjct: 188 AGIGGMD 194
>At1g70570 unknown protein
Length = 595
Score = 28.9 bits (63), Expect = 8.8
Identities = 24/116 (20%), Positives = 43/116 (36%), Gaps = 16/116 (13%)
Query: 489 LTDINKAEKFIDETGIDALAVCIGN---------------VHGKYPASGPKLRLDLLKEL 533
L K ++F+ TG +A+ + VH G + L + +
Sbjct: 405 LATTEKVQQFVRATGKEAIVAGFYHEGYEEPLLMLMRRRGVHSGLVVKGEEGALSMTTRV 464
Query: 534 RAVSQKKGVLL-VLHGASGLGTELVKECINLGVRKFNVNTEVRKAYMDSLNTPKSD 588
RA S KG + G L ++ E + + FN+ + R + TP++D
Sbjct: 465 RAASASKGFPVNYCSGFRSLSSDTALEADGVSRQSFNLEVDARNYGFEPTETPRTD 520
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.133 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,932,212
Number of Sequences: 26719
Number of extensions: 531869
Number of successful extensions: 1500
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 1488
Number of HSP's gapped (non-prelim): 20
length of query: 617
length of database: 11,318,596
effective HSP length: 105
effective length of query: 512
effective length of database: 8,513,101
effective search space: 4358707712
effective search space used: 4358707712
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC138199.2