BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137823.6 + phase: 0
(217 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g38900 frnE protein - like 296 4e-81
At5g42020 luminal binding protein (dbj|BAA13948.1) 30 0.98
At5g28540 luminal binding protein 30 0.98
At2g17550 hypothetical protein 30 1.3
At5g27220 putative protein 29 2.2
At4g13080 endoxyloglucan transferase-like protein 28 2.8
At4g07370 28 4.8
At4g03230 putative receptor kinase 28 4.8
At3g07530 hypothetical protein 28 4.8
At1g56030 hypothetical protein 27 6.3
At5g44710 unknown protein 27 8.3
At5g11780 putative protein 27 8.3
At3g28344 P-glycoprotein, 5' partial 27 8.3
>At5g38900 frnE protein - like
Length = 217
Score = 296 bits (759), Expect = 4e-81
Identities = 140/217 (64%), Positives = 176/217 (80%)
Query: 1 MTDTNSAHSEKKLVRIDISSDTVCPWCFVGKKNLDKAIASSKDKYNFEILWHPYQLSPDA 60
M ++ S + KKL++ID+SSD+VCPWCFVGKKNLDKAI +SKD+YNFEI W P+ L P A
Sbjct: 1 MAESASNTASKKLIQIDVSSDSVCPWCFVGKKNLDKAIEASKDQYNFEIRWRPFFLDPSA 60
Query: 61 PKEGIEKREFYRSKFGSRSDQMEARMSEVFRTVGLVYSLSGLTGNTMDSHRLIYFSGQQG 120
PKEG+ K+EFY K+G+R M ARMSEVF+ +GL + +GLTGN++DSHRLI+++G+Q
Sbjct: 61 PKEGVSKKEFYLQKYGNRYQGMFARMSEVFKGLGLEFDTAGLTGNSLDSHRLIHYTGKQA 120
Query: 121 LDKQHDLVEELGLGYFTQEKYIGDQEFLLEAAAKVGIEGAEEFLKNPNNGLKEVEEELKT 180
+KQH LVEEL +GYFTQ K+IGD+EFL+E A KVGIEGAEEFL +PNNG+ EV+EEL
Sbjct: 121 PEKQHTLVEELFIGYFTQGKFIGDREFLVETANKVGIEGAEEFLSDPNNGVTEVKEELAK 180
Query: 181 YSRNITGVPYYVINGSQKLSGGQPPEVFLRAFQAATS 217
YS+NITGVP Y ING KLSG QPPE F AF+AA++
Sbjct: 181 YSKNITGVPNYTINGKVKLSGAQPPETFQSAFKAASA 217
>At5g42020 luminal binding protein (dbj|BAA13948.1)
Length = 668
Score = 30.0 bits (66), Expect = 0.98
Identities = 21/66 (31%), Positives = 34/66 (50%), Gaps = 7/66 (10%)
Query: 139 EKYIGDQEFLLEAAAKVGIEGAEEFLKNPNNGLKEVEEELKTYSRNITGVPYYVINGSQK 198
+K GD++ +EAA K +E +E N N+ +E +E+LK + V +I +
Sbjct: 592 DKLEGDEKEKIEAATKEALEWLDE---NQNSEKEEYDEKLK----EVEAVCNPIITAVYQ 644
Query: 199 LSGGQP 204
SGG P
Sbjct: 645 RSGGAP 650
>At5g28540 luminal binding protein
Length = 669
Score = 30.0 bits (66), Expect = 0.98
Identities = 21/66 (31%), Positives = 34/66 (50%), Gaps = 7/66 (10%)
Query: 139 EKYIGDQEFLLEAAAKVGIEGAEEFLKNPNNGLKEVEEELKTYSRNITGVPYYVINGSQK 198
+K GD++ +EAA K +E +E N N+ +E +E+LK + V +I +
Sbjct: 592 DKLEGDEKEKIEAATKEALEWLDE---NQNSEKEEYDEKLK----EVEAVCNPIITAVYQ 644
Query: 199 LSGGQP 204
SGG P
Sbjct: 645 RSGGAP 650
>At2g17550 hypothetical protein
Length = 765
Score = 29.6 bits (65), Expect = 1.3
Identities = 21/91 (23%), Positives = 41/91 (44%), Gaps = 9/91 (9%)
Query: 28 FVGKKNLDKAIASSKDKYNFEI-LWHPYQLSPDAPKEGIEKREFY-RSKFGSRS------ 79
F KN D+ + + ++ + ++ +H Y D P G E+ E+Y RS +
Sbjct: 20 FSHHKNRDEDLEAPRNSFELQVDNFHTYHNGKDKPSNGFEEEEWYERSCYPIEESMKKKI 79
Query: 80 -DQMEARMSEVFRTVGLVYSLSGLTGNTMDS 109
+++ R ++ T LV L G+ ++S
Sbjct: 80 IEELSKRSNDKQNTPSLVAKLMGMDALPLES 110
>At5g27220 putative protein
Length = 1181
Score = 28.9 bits (63), Expect = 2.2
Identities = 11/46 (23%), Positives = 31/46 (66%)
Query: 154 KVGIEGAEEFLKNPNNGLKEVEEELKTYSRNITGVPYYVINGSQKL 199
+V ++ +E L+ +NG +E+EEE++ ++++T V +++ +++
Sbjct: 164 RVEVKEEKEHLRRTDNGRRELEEEIERKTKDLTLVMNKIVDCDKRI 209
>At4g13080 endoxyloglucan transferase-like protein
Length = 295
Score = 28.5 bits (62), Expect = 2.8
Identities = 13/31 (41%), Positives = 20/31 (63%), Gaps = 4/31 (12%)
Query: 30 GKKNLDKAIA----SSKDKYNFEILWHPYQL 56
GK N ++ +A SKD + + ILW+PYQ+
Sbjct: 136 GKGNREQKLALWFDPSKDFHTYAILWNPYQI 166
>At4g07370
Length = 531
Score = 27.7 bits (60), Expect = 4.8
Identities = 18/76 (23%), Positives = 39/76 (50%), Gaps = 4/76 (5%)
Query: 116 SGQQGLDKQHDLVEELGLGYFTQEKYIGDQEF--LLEAAAKVGIEGAEEFLKNPNNGLKE 173
SG+ G L E L + + +G Q + L+E+ K+ ++ ++ P + +KE
Sbjct: 368 SGKPGYLPSETLSSEKSLE--SHKAAVGSQVYKGLMESDTKIIKANEDKAIQRPTHIVKE 425
Query: 174 VEEELKTYSRNITGVP 189
+++++K +R + VP
Sbjct: 426 LKDQIKELNRKLNQVP 441
>At4g03230 putative receptor kinase
Length = 852
Score = 27.7 bits (60), Expect = 4.8
Identities = 21/68 (30%), Positives = 32/68 (46%), Gaps = 7/68 (10%)
Query: 132 GLGYFTQEKYIGDQEFLLEAAAKVGIEGAEEFLKNPNNGLKEVEEELKTYSRNITGVPYY 191
G G + + GDQE ++ ++ +G EEF KN EV K RN+ + Y
Sbjct: 542 GFGPVYKGMFPGDQEIAVKRLSRCSGQGLEEF-KN------EVVLIAKLQHRNLVRLLGY 594
Query: 192 VINGSQKL 199
+ G +KL
Sbjct: 595 CVAGEEKL 602
>At3g07530 hypothetical protein
Length = 620
Score = 27.7 bits (60), Expect = 4.8
Identities = 20/67 (29%), Positives = 31/67 (45%), Gaps = 17/67 (25%)
Query: 146 EFLLEAAAKVGIEGAEE--------------FLKNPNNGLKEVEE---ELKTYSRNITGV 188
E LL+A K+GI+G+ E ++NPN+GL E E + T N+
Sbjct: 549 ELLLDALLKMGIKGSIEQSTGDNGSEDKSIIHIENPNSGLIEFSEMGTAIITGDENVVSQ 608
Query: 189 PYYVING 195
+ I+G
Sbjct: 609 VFQAIDG 615
>At1g56030 hypothetical protein
Length = 371
Score = 27.3 bits (59), Expect = 6.3
Identities = 10/20 (50%), Positives = 14/20 (70%)
Query: 27 CFVGKKNLDKAIASSKDKYN 46
CF G+KNL + + KDKY+
Sbjct: 247 CFTGQKNLKSQVITWKDKYD 266
>At5g44710 unknown protein
Length = 102
Score = 26.9 bits (58), Expect = 8.3
Identities = 19/74 (25%), Positives = 30/74 (39%)
Query: 1 MTDTNSAHSEKKLVRIDISSDTVCPWCFVGKKNLDKAIASSKDKYNFEILWHPYQLSPDA 60
M + S K++R + D V W KN D + + ++K L +
Sbjct: 29 MLNPTGQRSPHKILRKKLIGDKVAEWYPYDIKNEDPNVLAREEKERISKLEMLKRRDKGP 88
Query: 61 PKEGIEKREFYRSK 74
PK+G KR R+K
Sbjct: 89 PKKGHGKRAAKRNK 102
>At5g11780 putative protein
Length = 494
Score = 26.9 bits (58), Expect = 8.3
Identities = 13/41 (31%), Positives = 19/41 (45%)
Query: 144 DQEFLLEAAAKVGIEGAEEFLKNPNNGLKEVEEELKTYSRN 184
D+ FL E + I+ E+ NP NG + LK + N
Sbjct: 268 DEAFLTEIITEAVIKSVEKLFLNPGNGTSQRSLHLKNIAIN 308
>At3g28344 P-glycoprotein, 5' partial
Length = 626
Score = 26.9 bits (58), Expect = 8.3
Identities = 17/44 (38%), Positives = 20/44 (44%), Gaps = 3/44 (6%)
Query: 125 HDLVEELGLGYFTQEKYIGDQEFLLEAAAKVGIEGAEEFLKNPN 168
HD + L GY T Y GD+ L K I A LKNP+
Sbjct: 508 HDFITSLTEGYDT---YCGDRGVQLSGGQKQRIAIARAVLKNPS 548
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.314 0.134 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,931,785
Number of Sequences: 26719
Number of extensions: 209015
Number of successful extensions: 393
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 388
Number of HSP's gapped (non-prelim): 13
length of query: 217
length of database: 11,318,596
effective HSP length: 95
effective length of query: 122
effective length of database: 8,780,291
effective search space: 1071195502
effective search space used: 1071195502
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC137823.6