BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137553.4 - phase: 0 /pseudo
(921 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10580 putative reverse-transcriptase -like protein 94 3e-19
At2g07660 putative retroelement pol polyprotein 92 1e-18
At2g10780 pseudogene 92 1e-18
At2g04670 putative retroelement pol polyprotein 90 7e-18
At2g14650 putative retroelement pol polyprotein 67 4e-11
At3g11970 hypothetical protein 59 1e-08
At1g36590 hypothetical protein 59 1e-08
At2g05610 putative retroelement pol polyprotein 59 1e-08
At1g35370 hypothetical protein 59 2e-08
At1g36120 putative reverse transcriptase gb|AAD22339.1 50 8e-06
At4g04230 putative transposon protein 47 5e-05
orf107e -mitochondrial genome- 41 0.003
At4g07850 putative polyprotein 41 0.004
At3g31970 hypothetical protein 38 0.023
At3g31490 hypothetical protein 37 0.051
At4g03650 putative reverse transcriptase 36 0.087
At1g20390 hypothetical protein 36 0.087
At4g16910 retrotransposon like protein 36 0.11
At4g07830 putative reverse transcriptase 35 0.25
At4g03840 putative transposon protein 35 0.25
>At4g10580 putative reverse-transcriptase -like protein
Length = 1240
Score = 94.4 bits (233), Expect = 3e-19
Identities = 45/63 (71%), Positives = 53/63 (83%)
Query: 1 MSVSELAELKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDGSMRLRIDYRQLNKVTIKNR 60
M+ +E+AELKKQL+DL+ F RPS SPWGAPVL VKKKDGS RL IDYR+LN+VT+KNR
Sbjct: 499 MAPAEMAELKKQLKDLLGKGFIRPSTSPWGAPVLFVKKKDGSFRLCIDYRELNRVTVKNR 558
Query: 61 YLL 63
Y L
Sbjct: 559 YPL 561
Score = 36.2 bits (82), Expect = 0.087
Identities = 14/23 (60%), Positives = 20/23 (86%)
Query: 350 RFEVFSDHKTLKYLFDQKELNVR 372
+ +VF+DHK+LKY+F Q ELN+R
Sbjct: 848 KVQVFTDHKSLKYIFTQPELNLR 870
>At2g07660 putative retroelement pol polyprotein
Length = 949
Score = 92.4 bits (228), Expect = 1e-18
Identities = 45/63 (71%), Positives = 52/63 (82%)
Query: 1 MSVSELAELKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDGSMRLRIDYRQLNKVTIKNR 60
M+ +E+AELKKQLE+L+ F RPS SPWGAPVL VKKKDGS RL IDYR LNKVT+KN+
Sbjct: 162 MAPAEMAELKKQLEELLAKGFIRPSSSPWGAPVLFVKKKDGSFRLCIDYRGLNKVTVKNK 221
Query: 61 YLL 63
Y L
Sbjct: 222 YPL 224
>At2g10780 pseudogene
Length = 1611
Score = 92.0 bits (227), Expect = 1e-18
Identities = 44/63 (69%), Positives = 53/63 (83%)
Query: 1 MSVSELAELKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDGSMRLRIDYRQLNKVTIKNR 60
M+ +E+A+LKKQLE+L++ F RPS SPWGAPVL VKKKDGS RL IDYR LNKVT+KN+
Sbjct: 667 MAPAEMAKLKKQLEELLDKGFIRPSSSPWGAPVLFVKKKDGSFRLCIDYRGLNKVTVKNK 726
Query: 61 YLL 63
Y L
Sbjct: 727 YPL 729
Score = 34.7 bits (78), Expect = 0.25
Identities = 12/23 (52%), Positives = 20/23 (86%)
Query: 350 RFEVFSDHKTLKYLFDQKELNVR 372
+ ++++DHK+LKY+F Q ELN+R
Sbjct: 1016 KVQIYTDHKSLKYIFTQPELNLR 1038
>At2g04670 putative retroelement pol polyprotein
Length = 1411
Score = 89.7 bits (221), Expect = 7e-18
Identities = 44/63 (69%), Positives = 50/63 (78%)
Query: 1 MSVSELAELKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDGSMRLRIDYRQLNKVTIKNR 60
M+ +E+ ELKKQLEDL+ F RPS SPWGAPVL VKKKDGS RL IDYR LN VT+KN+
Sbjct: 525 MAPAEMTELKKQLEDLLGKGFIRPSTSPWGAPVLFVKKKDGSFRLCIDYRGLNWVTVKNK 584
Query: 61 YLL 63
Y L
Sbjct: 585 YPL 587
Score = 37.0 bits (84), Expect = 0.051
Identities = 14/23 (60%), Positives = 21/23 (90%)
Query: 350 RFEVFSDHKTLKYLFDQKELNVR 372
+ +VF+DHK+LKY+F+Q ELN+R
Sbjct: 874 KVQVFTDHKSLKYIFNQPELNLR 896
>At2g14650 putative retroelement pol polyprotein
Length = 1328
Score = 67.4 bits (163), Expect = 4e-11
Identities = 38/63 (60%), Positives = 44/63 (69%), Gaps = 12/63 (19%)
Query: 1 MSVSELAELKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDGSMRLRIDYRQLNKVTIKNR 60
M+ +E+AELKKQLEDL+ GAPVL VKKKDGS RL IDYR LN VT+KN+
Sbjct: 486 MAPAEMAELKKQLEDLL------------GAPVLFVKKKDGSFRLCIDYRGLNWVTVKNK 533
Query: 61 YLL 63
Y L
Sbjct: 534 YPL 536
Score = 35.8 bits (81), Expect = 0.11
Identities = 14/21 (66%), Positives = 19/21 (89%)
Query: 352 EVFSDHKTLKYLFDQKELNVR 372
+VF+DHK+LKY+F Q ELN+R
Sbjct: 825 QVFTDHKSLKYIFTQPELNLR 845
>At3g11970 hypothetical protein
Length = 1499
Score = 59.3 bits (142), Expect = 1e-08
Identities = 30/69 (43%), Positives = 48/69 (69%), Gaps = 3/69 (4%)
Query: 2 SVSELAELKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDGSMRLRIDYRQLNKVTIKNRY 61
S+ + E+ K +EDL+ + S SP+ +PV+LVKKKDG+ RL +DYR+LN +T+K+ +
Sbjct: 610 SIHQKNEIDKLVEDLLTNGTVQASSSPYASPVVLVKKKDGTWRLCVDYRELNGMTVKDSF 669
Query: 62 ---LLRELM 67
L+ +LM
Sbjct: 670 PIPLIEDLM 678
>At1g36590 hypothetical protein
Length = 1499
Score = 59.3 bits (142), Expect = 1e-08
Identities = 30/69 (43%), Positives = 48/69 (69%), Gaps = 3/69 (4%)
Query: 2 SVSELAELKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDGSMRLRIDYRQLNKVTIKNRY 61
S+ + E+ K +EDL+ + S SP+ +PV+LVKKKDG+ RL +DYR+LN +T+K+ +
Sbjct: 610 SIHQKNEIDKLVEDLLTNGTVQASSSPYASPVVLVKKKDGTWRLCVDYRELNGMTVKDSF 669
Query: 62 ---LLRELM 67
L+ +LM
Sbjct: 670 PIPLIEDLM 678
>At2g05610 putative retroelement pol polyprotein
Length = 780
Score = 58.9 bits (141), Expect = 1e-08
Identities = 29/68 (42%), Positives = 48/68 (69%), Gaps = 3/68 (4%)
Query: 3 VSELAELKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDGSMRLRIDYRQLNKVTIKNRY- 61
V + E+ K ++D++ + S SP+ +PV+LVKKKDG+ RL +DYR+LN +T+K+R+
Sbjct: 53 VHQKNEIDKIVDDMLASGTIQASSSPYASPVVLVKKKDGTWRLCVDYRELNGMTVKDRFP 112
Query: 62 --LLRELM 67
L+ +LM
Sbjct: 113 IPLIEDLM 120
>At1g35370 hypothetical protein
Length = 1447
Score = 58.5 bits (140), Expect = 2e-08
Identities = 26/61 (42%), Positives = 45/61 (73%)
Query: 3 VSELAELKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDGSMRLRIDYRQLNKVTIKNRYL 62
V + E+ K ++D+++ + S SP+ +PV+LVKKKDG+ RL +DY +LN +T+K+R+L
Sbjct: 590 VHQKDEIDKIVQDMIKSGTIQVSSSPFASPVVLVKKKDGTWRLCVDYTELNGMTVKDRFL 649
Query: 63 L 63
+
Sbjct: 650 I 650
>At1g36120 putative reverse transcriptase gb|AAD22339.1
Length = 1235
Score = 49.7 bits (117), Expect = 8e-06
Identities = 29/63 (46%), Positives = 35/63 (55%), Gaps = 18/63 (28%)
Query: 1 MSVSELAELKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDGSMRLRIDYRQLNKVTIKNR 60
M +E+AELKKQLEDL+ F RPS S WGAP LN+VT+KN+
Sbjct: 543 MVPAEIAELKKQLEDLLGKGFIRPSTSRWGAP------------------GLNRVTLKNK 584
Query: 61 YLL 63
Y L
Sbjct: 585 YPL 587
Score = 32.3 bits (72), Expect = 1.3
Identities = 13/23 (56%), Positives = 19/23 (82%)
Query: 350 RFEVFSDHKTLKYLFDQKELNVR 372
+ +VF+DHK+LK +F Q ELN+R
Sbjct: 764 KVQVFTDHKSLKDIFTQPELNLR 786
>At4g04230 putative transposon protein
Length = 315
Score = 47.0 bits (110), Expect = 5e-05
Identities = 22/46 (47%), Positives = 31/46 (66%)
Query: 21 FFRPSVSPWGAPVLLVKKKDGSMRLRIDYRQLNKVTIKNRYLLREL 66
+ R S+SP PVLLV KKDG+ R+ +D R +N +TIK R+ + L
Sbjct: 129 YIRESLSPCAVPVLLVPKKDGTWRMCLDCRAINNITIKYRHPIPRL 174
>orf107e -mitochondrial genome-
Length = 107
Score = 41.2 bits (95), Expect = 0.003
Identities = 17/33 (51%), Positives = 26/33 (78%)
Query: 9 LKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDG 41
LK L +++E + +PS+SP+ +PVLLV+KKDG
Sbjct: 45 LKNWLGEMLEARIIQPSISPYSSPVLLVQKKDG 77
>At4g07850 putative polyprotein
Length = 1138
Score = 40.8 bits (94), Expect = 0.004
Identities = 21/40 (52%), Positives = 26/40 (64%)
Query: 5 ELAELKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDGSMR 44
E EL+KQ+ +L+E S+SP PVLLV KKDGS R
Sbjct: 490 ETKELEKQVNELMERGHICESMSPCAVPVLLVPKKDGSWR 529
>At3g31970 hypothetical protein
Length = 1329
Score = 38.1 bits (87), Expect = 0.023
Identities = 17/33 (51%), Positives = 25/33 (75%)
Query: 340 RYGDITCMVRRFEVFSDHKTLKYLFDQKELNVR 372
R G +++R +VF+DHK+LKY+F Q ELN+R
Sbjct: 755 RVGLDCVLMQRGKVFTDHKSLKYIFTQPELNLR 787
>At3g31490 hypothetical protein
Length = 285
Score = 37.0 bits (84), Expect = 0.051
Identities = 14/23 (60%), Positives = 21/23 (90%)
Query: 350 RFEVFSDHKTLKYLFDQKELNVR 372
+ +VF+DHK+LKY+F Q+ELN+R
Sbjct: 88 KVQVFTDHKSLKYMFTQQELNLR 110
>At4g03650 putative reverse transcriptase
Length = 839
Score = 36.2 bits (82), Expect = 0.087
Identities = 14/23 (60%), Positives = 20/23 (86%)
Query: 350 RFEVFSDHKTLKYLFDQKELNVR 372
+ +VF+DHK+LKY+F Q ELN+R
Sbjct: 773 KVQVFTDHKSLKYIFTQPELNLR 795
>At1g20390 hypothetical protein
Length = 1791
Score = 36.2 bits (82), Expect = 0.087
Identities = 21/62 (33%), Positives = 34/62 (53%), Gaps = 5/62 (8%)
Query: 2 SVSELAELKKQLEDLVEMKFFRPSVSPWGAPVLLVKKKDGSMRLRIDYRQLNKVTIKNRY 61
+V+E E + ++E+K+ W A ++VKKK+G R+ +DY LNK K+ Y
Sbjct: 792 AVNEEVEKLLKAGQIIEVKY-----PEWLANPVVVKKKNGKWRVCVDYTDLNKACPKDSY 846
Query: 62 LL 63
L
Sbjct: 847 PL 848
>At4g16910 retrotransposon like protein
Length = 687
Score = 35.8 bits (81), Expect = 0.11
Identities = 13/23 (56%), Positives = 20/23 (86%)
Query: 350 RFEVFSDHKTLKYLFDQKELNVR 372
+ ++F+DHK+LKY+F Q ELN+R
Sbjct: 108 KVQIFTDHKSLKYIFTQPELNLR 130
>At4g07830 putative reverse transcriptase
Length = 611
Score = 34.7 bits (78), Expect = 0.25
Identities = 14/20 (70%), Positives = 18/20 (90%)
Query: 353 VFSDHKTLKYLFDQKELNVR 372
VF+DHK+LKY+F Q ELN+R
Sbjct: 31 VFTDHKSLKYIFTQLELNLR 50
>At4g03840 putative transposon protein
Length = 973
Score = 34.7 bits (78), Expect = 0.25
Identities = 12/23 (52%), Positives = 20/23 (86%)
Query: 350 RFEVFSDHKTLKYLFDQKELNVR 372
+ ++++DHK+LKY+F Q ELN+R
Sbjct: 466 KVQIYTDHKSLKYIFTQPELNLR 488
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.366 0.163 0.618
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,516,602
Number of Sequences: 26719
Number of extensions: 587717
Number of successful extensions: 3093
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3048
Number of HSP's gapped (non-prelim): 45
length of query: 921
length of database: 11,318,596
effective HSP length: 108
effective length of query: 813
effective length of database: 8,432,944
effective search space: 6855983472
effective search space used: 6855983472
T: 11
A: 40
X1: 14 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.6 bits)
S2: 65 (29.6 bits)
Medicago: description of AC137553.4