BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137546.6 - phase: 0
(181 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g52140 unknown protein 81 3e-16
At4g29110 putative protein 75 1e-14
At3g16330 unknown protein 70 6e-13
At4g32860 putative protein 67 5e-12
At5g49935 unknown protein 34 0.038
At4g02160 hypothetical protein 31 0.42
At3g26610 polygalacturonase, putative 30 0.93
At1g68350 hypothetical protein 30 0.93
At5g38700 putative protein 28 2.1
At4g17410 hypothetical protein 27 6.0
At5g61710 putative protein 27 7.8
At4g17330 G2484-1 protein 27 7.8
At3g27060 ribonucleotide reductase small subunit, putative 27 7.8
At3g23580 ribonucleoside-diphosphate reductase small chain 27 7.8
>At1g52140 unknown protein
Length = 208
Score = 80.9 bits (198), Expect = 3e-16
Identities = 66/209 (31%), Positives = 99/209 (46%), Gaps = 49/209 (23%)
Query: 10 VAKKLWNMVRVLLFIIRKGIAKSKTMVHLNLILKRGKLAGKALINTLMLNHQLYHSSFTC 69
++KKLWN+VR LL++IRKG++K+K + N LKRGK N + + H+ T
Sbjct: 7 ISKKLWNIVRFLLYMIRKGVSKNKLIADFNATLKRGK-------NLMFHQRRRVHAGSTA 59
Query: 70 RSDNNSFISPC----EYEFSCRNTPANPLRHSSRRFSKSRRQRHNDF-------SIMNNN 118
+ N+ + EYEFSC NTP S+ F R++ HN+ ++++
Sbjct: 60 SAALNATSATASSRQEYEFSCSNTPNYSFPFSNMAF--MRKKSHNNLFTCGQTPQTLDDD 117
Query: 119 IAVQKVFIEMMLN-NEKVE------AVANSPL-----------------APFTLE----D 150
+A + +E++ EK VA SP +PF L D
Sbjct: 118 VAAARAVLELLNGVGEKGNVTPADLTVALSPYFPGFGQTPLVRPLRVTDSPFPLTPENGD 177
Query: 151 EGNCHQVDIAAEEFINNFYKELNRQNRTI 179
N H VD AA++FI FYK LN+Q + I
Sbjct: 178 VANGH-VDKAADDFIKKFYKNLNQQKKMI 205
>At4g29110 putative protein
Length = 200
Score = 75.5 bits (184), Expect = 1e-14
Identities = 67/197 (34%), Positives = 96/197 (48%), Gaps = 43/197 (21%)
Query: 1 MEIEARQGVVAKKLWNMVRVLLFIIRKGIAKSKTMVHLNLILKRGKLAGKALINTLMLNH 60
ME+E V AK+LW +VR++ +++ G K+K M+ LNL+LKRG KA+ N
Sbjct: 12 MEMEQNAQVAAKRLWKVVRIVFCVLKTGTVKNKLMLDLNLMLKRG---NKAITNLRR--- 65
Query: 61 QLYHSSFTCRSDNNSFISPCEYEFSCRNTPANPLRHSSRRFSKSRRQRHNDFSIMNNNI- 119
SS T D +S S R +P S+R +R+ H + + +
Sbjct: 66 ---RSSSTGSHDVSS---------SSRVRDYDPFAFISKR----KRRVHGGYDNEEDAVE 109
Query: 120 -AVQKVFIEMMLNNEK----VEAVANSPL-------------APFTLEDEG-NCHQVDIA 160
AV+KVF E++ N+K E+ SPL +PF L+D G + H VD A
Sbjct: 110 AAVKKVF-ELLGENDKKTVATESARESPLIMSPAVRQLRVTDSPFPLDDGGDHDHVVDKA 168
Query: 161 AEEFINNFYKELNRQNR 177
AEEFI FYK L Q +
Sbjct: 169 AEEFIKKFYKNLKLQKK 185
>At3g16330 unknown protein
Length = 205
Score = 70.1 bits (170), Expect = 6e-13
Identities = 57/211 (27%), Positives = 90/211 (42%), Gaps = 54/211 (25%)
Query: 10 VAKKLWNMVRVLLFIIRKGIAKSKTMVHLNLILKRGKLAGKALINTLMLNHQLYHSSFTC 69
++KKL N+VR +L+++ KGI+K K + N LKRGK N + N + S
Sbjct: 7 ISKKLGNIVRFVLYMLHKGISKQKLLADFNATLKRGK-------NLMFHNRRRVPGSAVA 59
Query: 70 RSDNNSFISPCEYEFSCRNTPANPLRHSSRRFSKSRRQRHNDF-------SIMNNNIAVQ 122
N EYEFSC +TP + F K + HN ++++ +V
Sbjct: 60 SHPQN------EYEFSCSDTPNYTFPFNMAAFKK--KSHHNSLFSCGQAPPTLDDDTSVS 111
Query: 123 KVFIEMMLNNEKVEAVANSPL------------------------------APFTLEDEG 152
+ +E++ + + +N+P +PF L +EG
Sbjct: 112 RAVLELLNSGGDHDQGSNTPAFSIEALTALSPYLPGFGRSTSSVRPLRVTDSPFPLREEG 171
Query: 153 NC--HQVDIAAEEFINNFYKELNRQNRTIAS 181
+ VD AA+EFI FYK L +Q + I S
Sbjct: 172 DVANGHVDKAADEFIKKFYKNLYQQKKMIES 202
>At4g32860 putative protein
Length = 193
Score = 67.0 bits (162), Expect = 5e-12
Identities = 64/200 (32%), Positives = 97/200 (48%), Gaps = 32/200 (16%)
Query: 3 IEARQGVVAKKLWNMVRVLLFIIRKGIAKSK----TMVHLNLILKRGKLAGKALINTLML 58
+E V KKL ++ +++LF I+K S+ T + +L+ KRGK+ K+L +
Sbjct: 1 MEVCSTVTTKKLSSLAKLILFTIQKVSDASRHKLLTTLDPHLLAKRGKILRKSLNEAVST 60
Query: 59 NHQLYHSSFTCRSDNN-----SFISPC----EYEFSCRNTPANPLRHSSRRFSKSRRQR- 108
+H S TCR ++ SFISP EYEFSC +TP P R + SK RR
Sbjct: 61 SH----SRITCRPSDHQDVRSSFISPVPLQLEYEFSCSSTP--PRRSYATTVSKGRRSNG 114
Query: 109 -HNDFSIMNNNIAVQKVFIEMMLNNEKVEAVANSPLAPFTLEDEGN-------CHQVDIA 160
HN I N ++ +I + +++ + +A D + CH VD A
Sbjct: 115 SHNRPLI---NKRQRQAYIRYNTLPKVRDSIWDRHVAAAVFPDVASSTGTMESCH-VDRA 170
Query: 161 AEEFINNFYKELNRQNRTIA 180
AEEFI +FY++L Q +A
Sbjct: 171 AEEFIQSFYRQLRLQKWMMA 190
>At5g49935 unknown protein
Length = 235
Score = 34.3 bits (77), Expect = 0.038
Identities = 23/71 (32%), Positives = 35/71 (48%), Gaps = 5/71 (7%)
Query: 90 PANPLRHSSRRFSKSRRQRHNDFSIMNNNIAVQKVFIEMMLNNEKVEAVANSPLAPFTLE 149
P NPLR RF SR+ S + +A +E+ L E VE+V + + P+ +
Sbjct: 47 PCNPLRRGLSRFLSSRQLFRR--SKVVKAVATPDPILEVPLTEENVESVLDE-IRPYLMS 103
Query: 150 DEGN--CHQVD 158
D GN H++D
Sbjct: 104 DGGNVALHEID 114
>At4g02160 hypothetical protein
Length = 183
Score = 30.8 bits (68), Expect = 0.42
Identities = 12/28 (42%), Positives = 18/28 (63%)
Query: 150 DEGNCHQVDIAAEEFINNFYKELNRQNR 177
D+G +D AEEFI FY+++ QN+
Sbjct: 142 DDGGDEMIDAKAEEFIVRFYEQMRMQNQ 169
>At3g26610 polygalacturonase, putative
Length = 470
Score = 29.6 bits (65), Expect = 0.93
Identities = 30/125 (24%), Positives = 53/125 (42%), Gaps = 9/125 (7%)
Query: 27 KGIAKSKTMVHLNLIL----KRGKLAGKAL---INTLMLNHQLYHSSFTCRSDNNSFISP 79
+G K +T+V + IL K G +L +N ++H S T ++ +
Sbjct: 117 QGPCKEETVVQIEGILVAPEKIGSWPNSSLFQWLNFKWVSHLTIQGSGTLNGRGYNWWNL 176
Query: 80 CEYEFSCRNTPANPLRHSSRRFSKSRRQRHNDFSIMNNNIAVQKVFIE--MMLNNEKVEA 137
Y+ RN P++ ++ RF S D SI+N+ + K + +NN + +
Sbjct: 177 DTYQTQTRNKYIPPMKPTALRFYSSNNVTVRDISIVNSPLCHLKFDDSDGVKVNNITISS 236
Query: 138 VANSP 142
NSP
Sbjct: 237 PENSP 241
>At1g68350 hypothetical protein
Length = 162
Score = 29.6 bits (65), Expect = 0.93
Identities = 17/46 (36%), Positives = 22/46 (46%), Gaps = 2/46 (4%)
Query: 135 VEAVANSPLAPFTLEDEGNCHQVDIAAEEFINNFYKELNRQNRTIA 180
V + A L PF E + +D+ AE FI FY E+ Q R A
Sbjct: 119 VASPAREHLQPFDYFSEDD--SIDLKAERFIEKFYDEMRMQAREFA 162
>At5g38700 putative protein
Length = 161
Score = 28.5 bits (62), Expect = 2.1
Identities = 16/46 (34%), Positives = 24/46 (51%), Gaps = 6/46 (13%)
Query: 132 NEKVEAVANSPLAPFTLEDEGNCHQVDIAAEEFINNFYKELNRQNR 177
NEK + AN ++E VD+ AEEFI FY+++ Q +
Sbjct: 109 NEKCDRSANDD------QEEEEEKGVDVRAEEFIAKFYEQMKLQRQ 148
>At4g17410 hypothetical protein
Length = 744
Score = 26.9 bits (58), Expect = 6.0
Identities = 15/39 (38%), Positives = 22/39 (55%), Gaps = 8/39 (20%)
Query: 81 EYEFSCRNTPANPLRHSSRRFS--------KSRRQRHND 111
+Y+ R+ ++P R SSRRF+ KS R RH+D
Sbjct: 569 DYDRRRRSDRSSPERQSSRRFTSPPRSSSRKSERDRHHD 607
>At5g61710 putative protein
Length = 155
Score = 26.6 bits (57), Expect = 7.8
Identities = 11/27 (40%), Positives = 16/27 (58%)
Query: 151 EGNCHQVDIAAEEFINNFYKELNRQNR 177
+G +D AEEFI FY ++ QN+
Sbjct: 114 QGGDDMIDEKAEEFIVRFYAQMKMQNQ 140
>At4g17330 G2484-1 protein
Length = 2037
Score = 26.6 bits (57), Expect = 7.8
Identities = 22/78 (28%), Positives = 36/78 (45%), Gaps = 11/78 (14%)
Query: 70 RSDNNSFISPCEYEFSCRNTPANPLRHSSRRFSKSRRQRHNDFSIMNNNIAVQKVFIEMM 129
+S +S +S +T P +HS+R KSR+++ S+ + +
Sbjct: 1151 QSGTSSNVSKVTPTLEPTSTVVAPAQHSTR--VKSRKRKKMPVSVESG---------PNI 1199
Query: 130 LNNEKVEAVANSPLAPFT 147
LN+ K +A SPL PFT
Sbjct: 1200 LNSLKQTELAASPLVPFT 1217
>At3g27060 ribonucleotide reductase small subunit, putative
Length = 332
Score = 26.6 bits (57), Expect = 7.8
Identities = 14/51 (27%), Positives = 23/51 (44%)
Query: 19 RVLLFIIRKGIAKSKTMVHLNLILKRGKLAGKALINTLMLNHQLYHSSFTC 69
R++ F +GI S + + + KRG + G N L+ + H F C
Sbjct: 161 RIIAFACVEGIFFSGSFCSIFWLKKRGLMPGLTFSNELISRDEGLHCDFAC 211
>At3g23580 ribonucleoside-diphosphate reductase small chain
Length = 341
Score = 26.6 bits (57), Expect = 7.8
Identities = 15/52 (28%), Positives = 24/52 (45%)
Query: 18 VRVLLFIIRKGIAKSKTMVHLNLILKRGKLAGKALINTLMLNHQLYHSSFTC 69
VR++ F +GI S + + + KRG + G N L+ + H F C
Sbjct: 171 VRLVAFACVEGIFFSGSFCAIFWLKKRGLMPGLTFSNELISRDEGLHCDFAC 222
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,747,247
Number of Sequences: 26719
Number of extensions: 145622
Number of successful extensions: 340
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 324
Number of HSP's gapped (non-prelim): 14
length of query: 181
length of database: 11,318,596
effective HSP length: 93
effective length of query: 88
effective length of database: 8,833,729
effective search space: 777368152
effective search space used: 777368152
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC137546.6