BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136954.11 + phase: 0
(174 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57685 putative protein 132 7e-32
At4g25760 unknown protein 117 3e-27
At4g31730 hypothetical protein 115 9e-27
At5g24920 putative protein 109 9e-25
At2g24762 unknown protein 107 4e-24
At3g29710 hypothetical protein 37 0.005
At4g37820 unknown protein 35 0.021
At5g38770 putative protein 35 0.027
At1g05490 hypothetical protein 33 0.10
At2g44940 putative AP2 domain transcription factor 32 0.17
At5g63480 unknown protein 31 0.39
At2g17880 putative DnaJ protein 31 0.39
At1g16190 hypothetical protein, 3' partial 31 0.39
At5g10550 bromodomain protein - like 30 0.51
At4g04980 30 0.66
At3g62630 unknown protein 30 0.66
At3g24660 putative kinase-like protein TMKL1 precursor 30 0.66
At1g61730 unknown protein 30 0.66
At3g03130 hypothetical protein 30 0.86
At1g73660 putative protein kinase 30 0.86
>At5g57685 putative protein
Length = 157
Score = 132 bits (333), Expect = 7e-32
Identities = 74/121 (61%), Positives = 81/121 (66%), Gaps = 23/121 (19%)
Query: 21 PHSPWHSPVPYLFGGLAAMLGLIAFALLILACSYWKLSGYLEG--NGESERDLEAGERNN 78
PHSPWHSPVPYLFGGLAAMLGLIAFALLILACSYW+LSGYL+G N ERDLE G+
Sbjct: 24 PHSPWHSPVPYLFGGLAAMLGLIAFALLILACSYWRLSGYLDGEENQSRERDLEVGDVKP 83
Query: 79 D-TDQKP--YEEKILVIMAGQDKPTFLATPSMSSSTSTSTSRSSSFGDNTSTCTCEHDKD 135
D T KP EK LVIMAG KPT+LATPS+ TCTC+ D D
Sbjct: 84 DKTAVKPVALPEKFLVIMAGNVKPTYLATPSV------------------KTCTCDDDDD 125
Query: 136 Q 136
+
Sbjct: 126 E 126
>At4g25760 unknown protein
Length = 129
Score = 117 bits (293), Expect = 3e-27
Identities = 70/140 (50%), Positives = 88/140 (62%), Gaps = 32/140 (22%)
Query: 4 YREPMNVSERAPISSQVPHSPWHSPVPYLFGGLAAMLGLIAFALLILACSYWKLSGYLEG 63
Y++ +N S S VPHSPWHSPVPYLFGGLAAML LI ALLILACSYW+LS
Sbjct: 11 YQDSINASS----SMVVPHSPWHSPVPYLFGGLAAMLALICVALLILACSYWRLS----- 61
Query: 64 NGESERDLEAGE---RNNDTDQKPY---EEKILVIMAGQDKPTFLATPSMSSSTSTSTSR 117
G +ERDLEAG+ +NDT++ + EK LVIMAG +PT+LATP+ S S
Sbjct: 62 -GSAERDLEAGDDAKPDNDTNKTKHTEMPEKFLVIMAGDVRPTYLATPATRSEQS----- 115
Query: 118 SSSFGDNTSTCTC-EHDKDQ 136
CTC +H++++
Sbjct: 116 ----------CTCGDHNEEE 125
>At4g31730 hypothetical protein
Length = 158
Score = 115 bits (289), Expect = 9e-27
Identities = 65/134 (48%), Positives = 78/134 (57%), Gaps = 9/134 (6%)
Query: 21 PHSPWHSPVPYLFGGLAAMLGLIAFALLILACSYWKLSGYLEGNGESERDLEAGERNNDT 80
P SPWHSPVPYLFGGLAAMLGLIAFALLILACSYW+LS E +G++ + + +
Sbjct: 26 PQSPWHSPVPYLFGGLAAMLGLIAFALLILACSYWRLSSSGEEDGQNVDEEKESRSGDKA 85
Query: 81 DQKPYEEKILVIMAGQDKPTFLATPSMSSSTSTSTSRSSSFGDNTSTCTCEHDKDQKSIE 140
YEEK LVIMAG+D P +LATP+M T G + + K E
Sbjct: 86 ANGAYEEKFLVIMAGEDLPRYLATPAMKKCTCG--------GHEGKMVISQEESVAKEEE 137
Query: 141 NMND-EESTVKQGG 153
M + EE VK G
Sbjct: 138 KMREGEEEKVKDTG 151
>At5g24920 putative protein
Length = 147
Score = 109 bits (272), Expect = 9e-25
Identities = 61/117 (52%), Positives = 76/117 (64%), Gaps = 9/117 (7%)
Query: 23 SPWHSPVPYLFGGLAAMLGLIAFALLILACSYWKLSGYLEGNGESERDLEAGERNNDTDQ 82
SPW +PVPYLFGGLAAMLGLIAFALL+LACSYW+LS E + E+ E+GE+
Sbjct: 8 SPWRTPVPYLFGGLAAMLGLIAFALLLLACSYWRLSRQTE---DEEKQTESGEK---VVA 61
Query: 83 KPYEEKILVIMAGQDKPTFLATPSMSSSTSTSTSRSSSFGDNTSTCTCEHDKDQKSI 139
K +EEKILVIMAGQ+ PTFLATP + + G N + +H K ++ I
Sbjct: 62 KAFEEKILVIMAGQNNPTFLATPVAAKICLDCVNMEKKEGQNGES---KHSKLEEHI 115
>At2g24762 unknown protein
Length = 156
Score = 107 bits (266), Expect = 4e-24
Identities = 62/131 (47%), Positives = 74/131 (56%), Gaps = 3/131 (2%)
Query: 21 PHSPWHSPVPYLFGGLAAMLGLIAFALLILACSYWKLSGYLEGNGESERDLEAGERNNDT 80
P SPWHSPVPYLFGGLAAMLGLIAFALLILACSYW+LS + +GE + +
Sbjct: 29 PISPWHSPVPYLFGGLAAMLGLIAFALLILACSYWRLSTSGDDSGERVDEEKESRSGVKA 88
Query: 81 DQKPYEEKILVIMAGQDKPTFLATPSMSSSTSTSTSRSSSFGDNTSTCTCEHDKDQKSIE 140
EEK+LVIMAG D P FLATP+ + R F ++ E D+ E
Sbjct: 89 ASAACEEKVLVIMAGDDLPRFLATPAANKCMCGHEGRMVIFKEDGIGAGEEKMGDR---E 145
Query: 141 NMNDEESTVKQ 151
+ E T Q
Sbjct: 146 KAKENEETTSQ 156
>At3g29710 hypothetical protein
Length = 669
Score = 37.0 bits (84), Expect = 0.005
Identities = 27/92 (29%), Positives = 41/92 (44%), Gaps = 6/92 (6%)
Query: 62 EGNGESERDLEAGERNNDTDQKPYEEK-----ILVIMAGQDKPTFLATPSMSSSTSTSTS 116
EG GE A E + D ++ P EE+ MA PT A P+ ++ T+ T+
Sbjct: 45 EGAGEESEGSGAVEESEDKEKVPAEEQSPTQTTATAMATNAAPT-TAAPTTTAPTTAPTT 103
Query: 117 RSSSFGDNTSTCTCEHDKDQKSIENMNDEEST 148
S D+T +H ++ ENM +E T
Sbjct: 104 ESPMLDDSTFYDALKHIPAEEIQENMQTDEET 135
>At4g37820 unknown protein
Length = 532
Score = 35.0 bits (79), Expect = 0.021
Identities = 24/92 (26%), Positives = 47/92 (51%), Gaps = 6/92 (6%)
Query: 63 GNGESERDLEAGERNNDTDQKPYEEKILVIMAGQDKPTFLATPSMSSSTSTSTS-RSSSF 121
G+ + E+ E ++ T +K + + G+ T L+T + + +T +S S S S
Sbjct: 230 GSDDGEQQETKSELDSKTGEKGFSDS-----NGELPETNLSTSNATETTESSGSDESGSS 284
Query: 122 GDNTSTCTCEHDKDQKSIENMNDEESTVKQGG 153
G +T ++++D+K ++EES VK+ G
Sbjct: 285 GKSTGYQQTKNEEDEKEKVQSSEEESKVKESG 316
>At5g38770 putative protein
Length = 97
Score = 34.7 bits (78), Expect = 0.027
Identities = 33/108 (30%), Positives = 54/108 (49%), Gaps = 17/108 (15%)
Query: 1 MAAYREPMNVSERAPISSQVPHSPWHSPVPYLFGGLAAMLGLIAFALLILACSYWKLSGY 60
M+ +R+ M P++S++ + SP+ MLGL FAL + A +Y + Y
Sbjct: 1 MSLHRDSM-----VPVNSRLENMD--SPILSKICAWGVMLGL--FALSLFAMAY---ACY 48
Query: 61 LEGNGESERDLEAGERN--NDTDQKPYEEKILVIMAGQDKPTFLATPS 106
+ S + + G++ D +P KI+VIMAG + PTF A P+
Sbjct: 49 HKQTSNSCIEEKQGKKQVLKPLDMEP---KIVVIMAGNENPTFFAKPT 93
>At1g05490 hypothetical protein
Length = 731
Score = 32.7 bits (73), Expect = 0.10
Identities = 22/90 (24%), Positives = 41/90 (45%), Gaps = 5/90 (5%)
Query: 62 EGNGESERDLEAGERNNDTDQKPYEEKILVIMAGQDKPTFLATPSMSSSTSTSTSRSSSF 121
+G S E E N D++ ++ + V + P+ S SSS+S+S+S SSS
Sbjct: 246 DGEDSSSETDEEEEENQDSEDNNTKDNVTVESLSSEDPS-----SSSSSSSSSSSSSSSS 300
Query: 122 GDNTSTCTCEHDKDQKSIENMNDEESTVKQ 151
+ + E D + +++ S +K+
Sbjct: 301 SSDDESYVKEVVGDNRDDDDLRKASSPIKR 330
>At2g44940 putative AP2 domain transcription factor
Length = 295
Score = 32.0 bits (71), Expect = 0.17
Identities = 21/67 (31%), Positives = 33/67 (48%), Gaps = 1/67 (1%)
Query: 92 IMAGQDKPTFLATPSMSSSTSTSTSRSSSFGDN-TSTCTCEHDKDQKSIENMNDEESTVK 150
I A + A+ S+SSS +TS+S SSS N + ++S+ ++ E
Sbjct: 21 IPAVSSSSSITASASLSSSPTTSSSSSSSTNSNFIEEDNSKRKASRRSLSSLVSVEDDDD 80
Query: 151 QGGGGGE 157
Q GGGG+
Sbjct: 81 QNGGGGK 87
>At5g63480 unknown protein
Length = 189
Score = 30.8 bits (68), Expect = 0.39
Identities = 20/78 (25%), Positives = 37/78 (46%), Gaps = 6/78 (7%)
Query: 82 QKPYEEKILVIMAGQDKPTFLATPSMSSSTSTSTSRSSSFGDNTSTCTCEHDKDQKSIEN 141
++ E +I A D+ L PS+ S+++T +S +++ G N S D +I+
Sbjct: 26 EETIEAAFQIISAMNDE---LCNPSLWSTSATPSSAATTTGSNGSALV---SADAAAIDG 79
Query: 142 MNDEESTVKQGGGGGENH 159
+ + GGGGG +
Sbjct: 80 TSHHSESAGGGGGGGSGN 97
>At2g17880 putative DnaJ protein
Length = 160
Score = 30.8 bits (68), Expect = 0.39
Identities = 19/47 (40%), Positives = 24/47 (50%), Gaps = 12/47 (25%)
Query: 99 PTFLATPSMSSSTSTSTSRSS------------SFGDNTSTCTCEHD 133
PTF ATP +SSS+S+S S SS S T++ TC D
Sbjct: 6 PTFFATPFLSSSSSSSPSFSSTSPPSRISRISPSLSTTTASYTCAED 52
>At1g16190 hypothetical protein, 3' partial
Length = 236
Score = 30.8 bits (68), Expect = 0.39
Identities = 17/40 (42%), Positives = 22/40 (54%)
Query: 81 DQKPYEEKILVIMAGQDKPTFLATPSMSSSTSTSTSRSSS 120
+ K EE LV+M + K A PS + TST+TS SS
Sbjct: 38 ENKVTEEGFLVVMLSKSKTASSAGPSSTQPTSTTTSTISS 77
>At5g10550 bromodomain protein - like
Length = 678
Score = 30.4 bits (67), Expect = 0.51
Identities = 22/70 (31%), Positives = 33/70 (46%)
Query: 72 EAGERNNDTDQKPYEEKILVIMAGQDKPTFLATPSMSSSTSTSTSRSSSFGDNTSTCTCE 131
EAGE + D + E + +D A+ SSS S S+S SSS D+ S +
Sbjct: 578 EAGEEDVDIGEDIPVEDYPSVEIERDGTAAAASGGSSSSGSFSSSGSSSSSDSESGSSSG 637
Query: 132 HDKDQKSIEN 141
D D S+++
Sbjct: 638 SDSDADSVQS 647
>At4g04980
Length = 681
Score = 30.0 bits (66), Expect = 0.66
Identities = 20/72 (27%), Positives = 29/72 (39%), Gaps = 14/72 (19%)
Query: 50 LACSYWKLSGYLEGNGESERDLEAGERNNDTDQKPYEEKILVIMAGQDKPTFLATPSMSS 109
+A YW L G LEG G + +A + N +K P +A M+
Sbjct: 397 IANLYWALKGKLEGRGVEGKTKKASKGQNSVAEK--------------SPVKVARSGMAD 442
Query: 110 STSTSTSRSSSF 121
+ + T RSS F
Sbjct: 443 ALAEMTKRSSYF 454
>At3g62630 unknown protein
Length = 380
Score = 30.0 bits (66), Expect = 0.66
Identities = 19/62 (30%), Positives = 32/62 (50%), Gaps = 4/62 (6%)
Query: 64 NGESERDLEAGERNNDTDQKPYEEKILVIMAGQDKPTFLATPSMSSSTSTSTSRSSSFGD 123
N + + E GE ++ +E I + ++ P+ TPS S+ S+SRSSS+G
Sbjct: 175 NAAYQWNQEEGEEEEVAGEREVKECIRKLQEDENVPSAETTPSCSA----SSSRSSSYGR 230
Query: 124 NT 125
N+
Sbjct: 231 NS 232
>At3g24660 putative kinase-like protein TMKL1 precursor
Length = 674
Score = 30.0 bits (66), Expect = 0.66
Identities = 26/94 (27%), Positives = 43/94 (45%), Gaps = 13/94 (13%)
Query: 39 MLGLIAFALLILACSYWKLSGYLEGNG-----ESERDLEAGERNNDTDQKPYEEKILVIM 93
++GL++ A+++ + L GYL+ ESE DLE G+ ++ +K E LV+
Sbjct: 305 VIGLMSGAVVVASL----LIGYLQNKKRKSSIESEDDLEEGDEEDEIGEKEGGEGKLVVF 360
Query: 94 AGQDKPT----FLATPSMSSSTSTSTSRSSSFGD 123
G + T AT + TS T + D
Sbjct: 361 QGGENLTLDDVLNATGQVMEKTSYGTVYKAKLSD 394
>At1g61730 unknown protein
Length = 376
Score = 30.0 bits (66), Expect = 0.66
Identities = 26/103 (25%), Positives = 40/103 (38%), Gaps = 16/103 (15%)
Query: 72 EAGERNNDTDQKPYEEKILVIMAGQDKPTFLATPSMSSSTSTSTSRSSSFGDNTSTCTCE 131
EAGE ++D+ + I + + T A P+ ST+ ST+ S G T T +
Sbjct: 26 EAGEASDDSSSSEEDVPIKIRIKSPSATTAAAPPA--KSTAVSTAADSDSGSETETDS-- 81
Query: 132 HDKDQKSIENMNDEESTVKQGGGGGENHVRRTESVETPTTDTS 174
D EST G G+ T +++ TS
Sbjct: 82 ------------DSESTNPPNSGSGKTIALNTVNLKKKEDPTS 112
>At3g03130 hypothetical protein
Length = 520
Score = 29.6 bits (65), Expect = 0.86
Identities = 26/109 (23%), Positives = 48/109 (43%), Gaps = 10/109 (9%)
Query: 61 LEGNGESERDLEAGERNNDTDQKPYEEKILVIMAGQDKPTFLATPSMSSSTSTSTSRSSS 120
LE + + D E GE + + EE I + ++ P +S + ++S SSS
Sbjct: 389 LEEDSDEWSDYEIGEVELEENSCGSEESIEI--ESEEAP-------VSDKKTPASSSSSS 439
Query: 121 FGDNTSTCTCEHDKDQKSIENMNDEESTVKQGG-GGGENHVRRTESVET 168
N + + + + +E+ D+E V G G E V++T+ +T
Sbjct: 440 LAGNETRTSLSPFEAESILESEEDKEMAVNNNGEGKAEAEVKKTKKKKT 488
>At1g73660 putative protein kinase
Length = 1030
Score = 29.6 bits (65), Expect = 0.86
Identities = 21/64 (32%), Positives = 29/64 (44%), Gaps = 10/64 (15%)
Query: 97 DKPTFLATPSMSSS---TSTSTSRSSSFGDNTSTCTCEHDKDQKSIENMNDEESTVKQGG 153
D+ + A+P + S S+S SS+ +NT T EH KS N Q G
Sbjct: 395 DESAYSASPGDNDSIHVASSSNGIESSYEENTEFRTGEHRSSTKSSGERN-------QSG 447
Query: 154 GGGE 157
GGG+
Sbjct: 448 GGGD 451
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.308 0.126 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,176,230
Number of Sequences: 26719
Number of extensions: 179002
Number of successful extensions: 1018
Number of sequences better than 10.0: 80
Number of HSP's better than 10.0 without gapping: 42
Number of HSP's successfully gapped in prelim test: 38
Number of HSP's that attempted gapping in prelim test: 929
Number of HSP's gapped (non-prelim): 98
length of query: 174
length of database: 11,318,596
effective HSP length: 92
effective length of query: 82
effective length of database: 8,860,448
effective search space: 726556736
effective search space used: 726556736
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC136954.11