
BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136838.2 - phase: 2
(90 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g16025 putative protein 28 0.79
At1g53570 MEK kinase MAP3Ka, putative 27 1.4
At3g12950 unknown protein 27 2.3
At5g57240 oxysterol-binding protein-like 26 3.9
At4g25650 unknown protein 26 3.9
At4g11420 putative protein 26 3.9
At2g40480 hypothetical protein 26 3.9
At1g63540 unknown protein 26 3.9
At5g36970 putative protein 25 5.1
At3g07960 putative phosphatidylinositol-4-phosphate 5-kinase 25 5.1
At4g32600 unknown protein 25 6.7
At3g07940 putative GTPase activating protein 25 6.7
At1g64450 unknown protein 25 6.7
At1g60020 hypothetical protein 25 8.8
At1g23240 unknown protein 25 8.8
At1g22810 TINY-like transcription factor 25 8.8
>At1g16025 putative protein
Length = 113
Score = 28.1 bits (61), Expect = 0.79
Identities = 16/48 (33%), Positives = 19/48 (39%)
Query: 16 PDSPTTPRGATGSEHNGALTLSGAPFQGTWARSAAEDASPYYNSNAEG 63
P P TPR + L S PF SA D S ++ SN G
Sbjct: 15 PFPPVTPRPPFPARKMSMLLSSSPPFMSLIVSSALVDGSSFWESNEMG 62
>At1g53570 MEK kinase MAP3Ka, putative
Length = 609
Score = 27.3 bits (59), Expect = 1.4
Identities = 13/36 (36%), Positives = 18/36 (49%), Gaps = 1/36 (2%)
Query: 12 IPKQPDSPTTPRGATGSEHNGALTLSGAPFQGTWAR 47
+P+ P SPT+P GS G S + F TW +
Sbjct: 182 LPRPPTSPTSPSAVHGSRIGGGYETSPSGF-STWKK 216
>At3g12950 unknown protein
Length = 558
Score = 26.6 bits (57), Expect = 2.3
Identities = 15/45 (33%), Positives = 20/45 (44%), Gaps = 1/45 (2%)
Query: 21 TPRGATGSEHNGALTLSGAPFQGTWARSAAED-ASPYYNSNAEGD 64
T GA EH T SG W +A ED + + N + +GD
Sbjct: 486 TKGGAPSVEHQFMPTFSGQCSASAWPETAREDLVAGFTNGSCDGD 530
>At5g57240 oxysterol-binding protein-like
Length = 379
Score = 25.8 bits (55), Expect = 3.9
Identities = 8/15 (53%), Positives = 9/15 (59%)
Query: 8 DWGCIPKQPDSPTTP 22
DW C+P QP P P
Sbjct: 360 DWDCVPLQPTVPRAP 374
>At4g25650 unknown protein
Length = 536
Score = 25.8 bits (55), Expect = 3.9
Identities = 17/71 (23%), Positives = 25/71 (34%), Gaps = 2/71 (2%)
Query: 4 NLPPDWGCIPKQPDSPTTPRGATGSEHNGALTLSGAPFQGTWARSAAEDASPYYNSNAEG 63
N P + C P P +P + + T +P + A S P
Sbjct: 17 NTKPRFRCSFSNPSLPISPNSLITRKSSRFTTAVSSPPSSSAATSTNSPPEPEALFEPGS 76
Query: 64 DRFSWWA--YP 72
D+F W+A YP
Sbjct: 77 DKFDWYANWYP 87
>At4g11420 putative protein
Length = 987
Score = 25.8 bits (55), Expect = 3.9
Identities = 19/67 (28%), Positives = 26/67 (38%), Gaps = 6/67 (8%)
Query: 2 GRNLPPDWGCIPKQPDSPTTPRGATGSEHNGALT-LSGAPFQGTWARSAAEDASPYYNSN 60
G N PP + + TP G T A AP+ W R E + P ++
Sbjct: 861 GTNAPP-----ARLAEPTVTPVGTTAPAAAAAAAGAPAAPYVPKWKRQTTEVSGPSAPTS 915
Query: 61 AEGDRFS 67
+E DR S
Sbjct: 916 SETDRRS 922
>At2g40480 hypothetical protein
Length = 541
Score = 25.8 bits (55), Expect = 3.9
Identities = 9/14 (64%), Positives = 10/14 (71%)
Query: 11 CIPKQPDSPTTPRG 24
C P+ P SP TPRG
Sbjct: 337 CFPEPPRSPVTPRG 350
>At1g63540 unknown protein
Length = 635
Score = 25.8 bits (55), Expect = 3.9
Identities = 16/57 (28%), Positives = 26/57 (45%), Gaps = 1/57 (1%)
Query: 19 PTTPRGATGSEHNGALTLSGAPFQGTWARSAAEDA-SPYYNSNAEGDRFSWWAYPGS 74
P+TP + + + T S W+R + + +P Y EGD S W++P S
Sbjct: 438 PSTPFSLSPAFSSNTNTGSYPFASHEWSRPTEQGSMNPGYAPTHEGDNSSGWSFPTS 494
>At5g36970 putative protein
Length = 248
Score = 25.4 bits (54), Expect = 5.1
Identities = 9/18 (50%), Positives = 12/18 (66%)
Query: 13 PKQPDSPTTPRGATGSEH 30
P P +P PRG++ SEH
Sbjct: 16 PPHPTAPLVPRGSSRSEH 33
>At3g07960 putative phosphatidylinositol-4-phosphate 5-kinase
Length = 715
Score = 25.4 bits (54), Expect = 5.1
Identities = 13/39 (33%), Positives = 19/39 (48%), Gaps = 2/39 (5%)
Query: 31 NGALTLS-GAPFQGTWARSAAEDASPYYNSNAEGDRFSW 68
NG S G+ + G W++ E YY S + G+ F W
Sbjct: 182 NGTFRWSDGSFYVGVWSKDPKEQNGTYYPSTSSGN-FDW 219
>At4g32600 unknown protein
Length = 453
Score = 25.0 bits (53), Expect = 6.7
Identities = 14/62 (22%), Positives = 30/62 (47%), Gaps = 6/62 (9%)
Query: 29 EHNGALTLSGAPFQGTWARSAAEDASPYYNSNAEGDRFSWWAYPGSLAVTK-GILVSFFS 87
+H L ++ PF + +R++ D +++ G R YPG ++ + ++V +F
Sbjct: 178 QHRPNLNVAAGPFAFSISRTSEADGRQTNTTSSRGSR-----YPGFISAARLKVIVEYFK 232
Query: 88 SA 89
A
Sbjct: 233 MA 234
>At3g07940 putative GTPase activating protein
Length = 385
Score = 25.0 bits (53), Expect = 6.7
Identities = 12/54 (22%), Positives = 23/54 (42%), Gaps = 1/54 (1%)
Query: 11 CIPKQPD-SPTTPRGATGSEHNGALTLSGAPFQGTWARSAAEDASPYYNSNAEG 63
C +QP + T+P + H G F+ +W R ++ P +++ G
Sbjct: 170 CTYQQPSRTNTSPPSLCSASHRSTKNRIGHAFRNSWGRRESDHKGPKKSNSMAG 223
>At1g64450 unknown protein
Length = 342
Score = 25.0 bits (53), Expect = 6.7
Identities = 9/17 (52%), Positives = 11/17 (63%)
Query: 6 PPDWGCIPKQPDSPTTP 22
PPD+ P+ PDSP P
Sbjct: 153 PPDFTVTPRNPDSPYFP 169
>At1g60020 hypothetical protein
Length = 1194
Score = 24.6 bits (52), Expect = 8.8
Identities = 12/34 (35%), Positives = 18/34 (52%)
Query: 27 GSEHNGALTLSGAPFQGTWARSAAEDASPYYNSN 60
GS +N ++ +PF R+ A ASPY +N
Sbjct: 254 GSSYNSQQSIPASPFTPWQPRANAAIASPYNANN 287
>At1g23240 unknown protein
Length = 184
Score = 24.6 bits (52), Expect = 8.8
Identities = 11/43 (25%), Positives = 22/43 (50%)
Query: 48 SAAEDASPYYNSNAEGDRFSWWAYPGSLAVTKGILVSFFSSAY 90
+A E +++ N +G + W Y G A+ G L++ F + +
Sbjct: 2 TALEKHVSFFDRNKDGTVYPWETYQGFRALGTGRLLAAFVAIF 44
>At1g22810 TINY-like transcription factor
Length = 144
Score = 24.6 bits (52), Expect = 8.8
Identities = 11/45 (24%), Positives = 18/45 (39%)
Query: 18 SPTTPRGATGSEHNGALTLSGAPFQGTWARSAAEDASPYYNSNAE 62
S T+PR + N + + T S D + YY + A+
Sbjct: 81 SRTSPRSIQQAASNAGMAIDAGIVHSTSVNSGCGDTTTYYENGAD 125
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.313 0.132 0.435
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,363,327
Number of Sequences: 26719
Number of extensions: 90505
Number of successful extensions: 162
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 149
Number of HSP's gapped (non-prelim): 19
length of query: 90
length of database: 11,318,596
effective HSP length: 66
effective length of query: 24
effective length of database: 9,555,142
effective search space: 229323408
effective search space used: 229323408
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC136838.2