BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136504.7 + phase: 0
(261 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35930 unknown protein 272 2e-73
At1g61340 unknown protein 67 1e-11
At4g21510 unknown protein 63 2e-10
At4g05010 54 6e-08
At2g29550 tubulin beta-7 chain 32 0.26
At1g34260 hypothetical protein 32 0.26
At4g02210 unknown protein 30 1.7
At1g63490 RB-binding protein -like 29 2.2
At4g19930 putative protein 28 3.8
At5g44340 tubulin beta-4 chain (sp|P24636) 28 5.0
At5g42640 C2H2-type zinc finger protein-like 28 5.0
At3g28790 unknown protein 28 5.0
At1g35660 unknown protein 28 6.5
At1g05080 hypothetical protein 28 6.5
At1g61620 unknown protein 27 8.5
At1g27650 U2 snRNP auxiliary factor, putative 27 8.5
>At4g35930 unknown protein
Length = 321
Score = 272 bits (695), Expect = 2e-73
Identities = 160/305 (52%), Positives = 195/305 (63%), Gaps = 55/305 (18%)
Query: 10 RRKLKKSGTKYLKPGTLAQLRCSKYLGSGS----GAGRV-------CNDIGKKRVELLNL 58
++KLK S KYLKPG L QL SK + S G RV CND+GKKRV + ++
Sbjct: 17 KKKLKSSSNKYLKPGALVQLCYSKASAAKSCNELGKKRVPVFDTKSCNDLGKKRVPVFDI 76
Query: 59 EKNEDHEGKVFD----KSPLMLSPVNLVKQSSSVG------------------------- 89
+ +++ V KSPLMLSPVN+VK+S+ V
Sbjct: 77 KHARNNKMAVEQLNSPKSPLMLSPVNVVKRSTLVRPMKFDDLQVESNNTCKKSPLMLSPM 136
Query: 90 ----------TPKTPRIDECQSESRLESLPIDLLVKILCCLHHDQLRAVFHVSQRIRKAV 139
TPKTP+ D C SES+LESLP+DLLVKI+C LHHDQL+AVFHVSQRIR A
Sbjct: 137 GIVMQNTLLRTPKTPQADPCVSESQLESLPMDLLVKIVCHLHHDQLKAVFHVSQRIRMAT 196
Query: 140 IVARQFHFNYTTPDRSRLEMLSTMTPRPSEHWPFLCRGDGKGLRIPSPHTPKAPRHGPRP 199
I+ARQ+HFNYTTPDRSR EML MTP P WPF RGDG + SPHTPKAP+H PRP
Sbjct: 197 ILARQYHFNYTTPDRSRQEMLRVMTPVPINRWPFR-RGDGNPTMVSSPHTPKAPKHAPRP 255
Query: 200 PSRLKVSEMRQVTAVLFK-ESAFASRCMVPSVIPKPQC-KSVASN--RVLFCEDELCKAV 255
PSR K++EM+Q+TAVLF+ ++ F SRC+VPSV+ +P K +A RVLF EDELC+AV
Sbjct: 256 PSRTKLAEMKQITAVLFQDQTPFPSRCIVPSVLQRPTLFKPMAPKHPRVLFYEDELCQAV 315
Query: 256 AQNKL 260
AQN L
Sbjct: 316 AQNNL 320
>At1g61340 unknown protein
Length = 185
Score = 67.0 bits (162), Expect = 1e-11
Identities = 41/121 (33%), Positives = 67/121 (54%), Gaps = 15/121 (12%)
Query: 35 LGSGSGAGRVCNDIGKKRVELLNLEKNEDHEGKVFDKSPLMLSPVNLVKQSSSVGTPKTP 94
LG G + G+KR+ + + ++ ED + SPV + KT
Sbjct: 26 LGLGLEFVQYKRGFGRKRILISSGDEMEDS---------IFTSPVG-----KKLCDDKTT 71
Query: 95 RIDECQSESRLESLPIDLLVKILCCLHHDQLRAVFHVSQRIRKAVIVARQFHFNYTTPDR 154
+ E QS LE LP+D+LV+I+C + H+ L+ +FHVS+ IR+A ++A+Q HF Y+TP +
Sbjct: 72 SVAEGQSRE-LEDLPLDILVRIICGVEHEDLKQLFHVSKTIREATMIAKQSHFAYSTPRK 130
Query: 155 S 155
+
Sbjct: 131 T 131
>At4g21510 unknown protein
Length = 225
Score = 62.8 bits (151), Expect = 2e-10
Identities = 55/190 (28%), Positives = 91/190 (46%), Gaps = 35/190 (18%)
Query: 38 GSGAGRVCNDIGKKRVELLNLEKNEDH----EGKVFDKSPLMLSPVNLVKQSSSVGTPKT 93
G G+ R +G+KR+ + + + E V +SP + S +L +Q S + T
Sbjct: 59 GLGSVRFTRGLGRKRILISSCVRESLSRSAVEIPVVSESPPVKS--SLKRQRSRI----T 112
Query: 94 PRIDECQSESRLESLPIDLLVKILCCLHHDQLRAVFHVSQRIRKAVIVARQFHFNYTTPD 153
+SRLE LP DLL++++C + H+ L+++ VS+ IR+A +VA+ HF YTTP
Sbjct: 113 IVSSSSSEKSRLECLPQDLLIRVICGVDHEDLKSLKLVSKSIREASLVAKTLHFAYTTPK 172
Query: 154 RSR-------LEMLSTMTPRPSEHWPFLCRGDGKGLRIPSPHTPKAPRHGPRPPSRLKVS 206
++R LE +S + + P P APRH ++ K
Sbjct: 173 KTRAFRNSIDLEEVSDSRHQEDDIEP-----------------PNAPRHYRWTKAKRK-E 214
Query: 207 EMRQVTAVLF 216
++ V+A LF
Sbjct: 215 QLSSVSAALF 224
>At4g05010
Length = 164
Score = 54.3 bits (129), Expect = 6e-08
Identities = 28/70 (40%), Positives = 44/70 (62%), Gaps = 2/70 (2%)
Query: 85 SSSVGTPKTPRIDECQSESRLESLPIDLLVKILCCLHHDQLRAVFHVSQRIRKAVIVARQ 144
S V P + C+S LE+L D+L+++LC + H+ L + VS+ IRKAVI A++
Sbjct: 46 SPPVKRPSHETTESCRS--LLETLHQDILIRVLCHVDHEDLATLKRVSKTIRKAVIEAKK 103
Query: 145 FHFNYTTPDR 154
HF+Y+TP +
Sbjct: 104 SHFDYSTPKK 113
>At2g29550 tubulin beta-7 chain
Length = 449
Score = 32.3 bits (72), Expect = 0.26
Identities = 18/48 (37%), Positives = 24/48 (49%)
Query: 42 GRVCNDIGKKRVELLNLEKNEDHEGKVFDKSPLMLSPVNLVKQSSSVG 89
G+ N IG K E++NLE D G+ S L L VN+ +S G
Sbjct: 10 GQCGNQIGSKFWEVVNLEHGIDQTGRYVGDSELQLERVNVYYNEASCG 57
>At1g34260 hypothetical protein
Length = 1456
Score = 32.3 bits (72), Expect = 0.26
Identities = 33/89 (37%), Positives = 37/89 (41%), Gaps = 6/89 (6%)
Query: 30 RCSKYLGSGSGAGRVCNDIGKKRVELLNL----EKNEDHEGKVFDKSPLMLSPVNLVKQS 85
R SKYLG V + NL E ED K DK PL+L P V S
Sbjct: 518 RLSKYLGFVQNPESVPVSVDTDVSTTSNLDSIRESEEDTAEKNEDKQPLLLDPELPVNSS 577
Query: 86 SSVGTPKTPRIDECQSESRLESLPIDLLV 114
S G K+ E ES LES I +LV
Sbjct: 578 SDDGDNKSQ--TENDIESTLESQSILVLV 604
>At4g02210 unknown protein
Length = 439
Score = 29.6 bits (65), Expect = 1.7
Identities = 23/70 (32%), Positives = 35/70 (49%), Gaps = 12/70 (17%)
Query: 70 DKSPLMLSPVNLVKQSSSVGT----PKTPRIDECQSES------RLESLP--IDLLVKIL 117
D + L+ P N Q ++ T PK PR+DE Q+ S +++LP D L+
Sbjct: 346 DSNSLLFDPKNKRDQLANTDTSPINPKKPRVDETQTMSIEDTVEAIQALPDMDDELILDA 405
Query: 118 CCLHHDQLRA 127
C L D+L+A
Sbjct: 406 CDLLEDKLKA 415
>At1g63490 RB-binding protein -like
Length = 1458
Score = 29.3 bits (64), Expect = 2.2
Identities = 21/84 (25%), Positives = 37/84 (44%), Gaps = 3/84 (3%)
Query: 42 GRVCNDIGKKRV--ELLNL-EKNEDHEGKVFDKSPLMLSPVNLVKQSSSVGTPKTPRIDE 98
G CN+ G + ELL + K + +++ L SP+ + + + SVG + P
Sbjct: 561 GNCCNNEGSIHLKKELLRIYSKEKTWREQLWKSGILRSSPMFVPECADSVGIEEDPTCII 620
Query: 99 CQSESRLESLPIDLLVKILCCLHH 122
CQ L ++ + + CL H
Sbjct: 621 CQQFLHLSAIVCNCRPSVFACLEH 644
>At4g19930 putative protein
Length = 431
Score = 28.5 bits (62), Expect = 3.8
Identities = 19/64 (29%), Positives = 33/64 (50%), Gaps = 1/64 (1%)
Query: 82 VKQSSSVGTPKTPRIDECQSESRLESLPIDLLVKILCCLHHDQLRAVFHVSQRIRKAVIV 141
+K+ S V + R D C+S + +P DL+++IL L L VS ++ ++I
Sbjct: 17 MKRKSRVTIRRRRRRDMCKSHEPMPYIPFDLVIEILTRLPAKSLMRFKSVS-KLWSSLIC 75
Query: 142 ARQF 145
+R F
Sbjct: 76 SRTF 79
>At5g44340 tubulin beta-4 chain (sp|P24636)
Length = 444
Score = 28.1 bits (61), Expect = 5.0
Identities = 16/48 (33%), Positives = 24/48 (49%)
Query: 42 GRVCNDIGKKRVELLNLEKNEDHEGKVFDKSPLMLSPVNLVKQSSSVG 89
G+ N IG K E++ E DH G+ SPL L +++ +S G
Sbjct: 10 GQCGNQIGAKFWEVICDEHGIDHTGQYVGDSPLQLERIDVYFNEASGG 57
>At5g42640 C2H2-type zinc finger protein-like
Length = 300
Score = 28.1 bits (61), Expect = 5.0
Identities = 22/79 (27%), Positives = 35/79 (43%), Gaps = 9/79 (11%)
Query: 78 PVNLVKQSSSVGTPKTPRIDECQSESRLESLPIDLLVKILCCLHHDQLRAVFHVSQRIRK 137
P NL ++S+S + + +ID Q + R SLP C + VF SQ+
Sbjct: 205 PTNLFEKSNSYDSQEDEKIDAYQYDGRTHSLPYTKYGPYTC----PRCNGVFDTSQKFAA 260
Query: 138 AVIVARQFHFNYTTPDRSR 156
++ H+N T D+ R
Sbjct: 261 HML----SHYNNET-DKER 274
>At3g28790 unknown protein
Length = 608
Score = 28.1 bits (61), Expect = 5.0
Identities = 21/70 (30%), Positives = 26/70 (37%)
Query: 150 TTPDRSRLEMLSTMTPRPSEHWPFLCRGDGKGLRIPSPHTPKAPRHGPRPPSRLKVSEMR 209
TT S + TP PS P P+P TP P P+ K SE
Sbjct: 266 TTGSSSGASPSGSPTPTPSTPTPSTPTPSTPTPSTPTPSTPTPSTPAPSTPAAGKTSEKG 325
Query: 210 QVTAVLFKES 219
+A + KES
Sbjct: 326 SESASMKKES 335
>At1g35660 unknown protein
Length = 1405
Score = 27.7 bits (60), Expect = 6.5
Identities = 20/84 (23%), Positives = 36/84 (42%), Gaps = 7/84 (8%)
Query: 31 CSKYLGSGSGAGRVCNDIGKKRVELLNLEKNEDHEG-------KVFDKSPLMLSPVNLVK 83
CS++ G VCN++G+ R+ L K ED +V D + ++L NL
Sbjct: 1017 CSEFQSVLRKKGWVCNELGRNRLGSKELNKAEDAFADAIVAFKEVCDHTNVILINCNLGH 1076
Query: 84 QSSSVGTPKTPRIDECQSESRLES 107
++ P+I+ + E+
Sbjct: 1077 GRRALAEEMVPKIEALELHRAFEN 1100
>At1g05080 hypothetical protein
Length = 439
Score = 27.7 bits (60), Expect = 6.5
Identities = 19/62 (30%), Positives = 28/62 (44%)
Query: 95 RIDECQSESRLESLPIDLLVKILCCLHHDQLRAVFHVSQRIRKAVIVARQFHFNYTTPDR 154
+I+E E R+ LP DLLV IL L + A +S+R + R + D
Sbjct: 5 KIEEIGCEDRISVLPEDLLVVILDLLPTKDVVATMILSKRWLSIWTMVRTLEYTDDMDDE 64
Query: 155 SR 156
S+
Sbjct: 65 SK 66
>At1g61620 unknown protein
Length = 310
Score = 27.3 bits (59), Expect = 8.5
Identities = 14/32 (43%), Positives = 19/32 (58%)
Query: 13 LKKSGTKYLKPGTLAQLRCSKYLGSGSGAGRV 44
LKK GT + + + + K+LGSGSG G V
Sbjct: 274 LKKGGTGFAEHDDHLEAKEYKHLGSGSGLGLV 305
>At1g27650 U2 snRNP auxiliary factor, putative
Length = 296
Score = 27.3 bits (59), Expect = 8.5
Identities = 16/58 (27%), Positives = 29/58 (49%), Gaps = 1/58 (1%)
Query: 9 GRRKLKKSGTKYLKPGTLAQLRCSKYLGSGSGAGRVCNDIGKKRVELLNLEKNEDHEG 66
G+R ++S + + G+ + + S G G GR ++ + R+E N E+ E EG
Sbjct: 238 GKRSSERS-ERQERDGSRGRRQASPKRGGSPGGGREGSEERRARIEQWNREREEKEEG 294
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.135 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,209,529
Number of Sequences: 26719
Number of extensions: 280934
Number of successful extensions: 842
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 824
Number of HSP's gapped (non-prelim): 19
length of query: 261
length of database: 11,318,596
effective HSP length: 97
effective length of query: 164
effective length of database: 8,726,853
effective search space: 1431203892
effective search space used: 1431203892
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC136504.7