BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136287.1 + phase: 1 /partial
(436 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g44160 methylenetetrahydrofolate reductase (MTHFR2) 739 0.0
At3g59970 methylenetetrahydrofolate reductase MTHFR1 727 0.0
At3g13490 lysyl-tRNA synthetase like protein 33 0.24
At2g42270 ATP-dependent RNA helicase like protein 32 0.90
At4g07590 31 1.5
At1g63220 putative elicitor-responsive gene 30 2.0
At1g42360 hypothetical protein 30 2.0
At2g06330 putative Athila retroelement ORF1 protein 29 4.5
At5g07480 putative protein 29 5.8
At1g42370 hypothetical protein 28 7.6
At1g02940 28 9.9
>At2g44160 methylenetetrahydrofolate reductase (MTHFR2)
Length = 594
Score = 739 bits (1907), Expect = 0.0
Identities = 346/433 (79%), Positives = 388/433 (88%)
Query: 1 AHPDVIGADGLATAEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITC 60
AHPDVIG +GLA+ E YQSDL YLK K+DAGADLIVTQLFYDTDIFLKFVNDCRQIGI+C
Sbjct: 156 AHPDVIGENGLASNEAYQSDLEYLKKKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISC 215
Query: 61 PIVPGIMPINNYKGFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIM 120
PIVPGIMPINNY+GF+RMTGFCKTKIP E+MAALEPIKDNEEAVKAYGIHLGTEMCKK++
Sbjct: 216 PIVPGIMPINNYRGFLRMTGFCKTKIPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKML 275
Query: 121 AHGIKTVHLYTLNMEKSALAILTSLGLIEESKISRSLPWRRPTNVFRVKEDVRPIFWANR 180
AHG+K++HLYTLNMEKSALAIL +LG+I+ESKISRSLPWRRP NVFR KEDVRPIFWANR
Sbjct: 276 AHGVKSLHLYTLNMEKSALAILMNLGMIDESKISRSLPWRRPANVFRTKEDVRPIFWANR 335
Query: 181 PKSYISRTIGWEQYPHGRWSDSGNPSYGALTDYQFMRPRAKDKKLIEEWAVPLKSIEDIY 240
PKSYISRT GWE +P GRW DS + SYGAL+D+QF RPRA+DKKL +EW VPLKS+EDI
Sbjct: 336 PKSYISRTKGWEDFPQGRWGDSRSASYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQ 395
Query: 241 ERFRLFCLGKLRTNPWSELDGLQPETKIINEHLEKINTKGFLTINSQPAVNGEKSDSPIV 300
E+F+ CLG L+++PWSELDGLQPET+IINE L K+N+KGFLTINSQP+VN E+SDSP V
Sbjct: 396 EKFKELCLGNLKSSPWSELDGLQPETRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTV 455
Query: 301 GWGGPGGYIYQKAYLEFFCSKEKLDALIEKSKDRPFLTYMAVNKEGIWKSNVGQTDVNAV 360
GWGGP GY+YQKAYLEFFCSKEKLDA++EK K P +TYMAVNK W SN Q DVNAV
Sbjct: 456 GWGGPVGYVYQKAYLEFFCSKEKLDAVVEKCKALPSITYMAVNKGEQWVSNTAQADVNAV 515
Query: 361 TWGVFPAKEIIQPTIVDPVSFNVWKDEAFEIWSRGWASLYPEADASRKLVEEVGSSYFLV 420
TWGVFPAKEIIQPTIVDP SFNVWKDEAFE WSR WA+LYPEAD SR L+EEV +SY+LV
Sbjct: 516 TWGVFPAKEIIQPTIVDPASFNVWKDEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLV 575
Query: 421 SLVDNDYANGDLF 433
SLV+NDY NGD+F
Sbjct: 576 SLVENDYINGDIF 588
>At3g59970 methylenetetrahydrofolate reductase MTHFR1
Length = 592
Score = 727 bits (1876), Expect = 0.0
Identities = 339/433 (78%), Positives = 381/433 (87%)
Query: 1 AHPDVIGADGLATAEGYQSDLAYLKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITC 60
AHPDVI ADGLAT E YQSDLAYLK KVDAGADLIVTQLFYDTDIFLKFVNDCRQIGI C
Sbjct: 156 AHPDVIEADGLATPESYQSDLAYLKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINC 215
Query: 61 PIVPGIMPINNYKGFIRMTGFCKTKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIM 120
PIVPGIMPI+NYKGF+RM GFCKTKIPAE+ AALEPIKDN+EAVKAYGIH TEMCKKI+
Sbjct: 216 PIVPGIMPISNYKGFLRMAGFCKTKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKIL 275
Query: 121 AHGIKTVHLYTLNMEKSALAILTSLGLIEESKISRSLPWRRPTNVFRVKEDVRPIFWANR 180
AHGI ++HLYTLN++KSA+ IL +LGLI+ESKISRSLPWRRP NVFR KEDVRPIFWANR
Sbjct: 276 AHGITSLHLYTLNVDKSAIGILMNLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANR 335
Query: 181 PKSYISRTIGWEQYPHGRWSDSGNPSYGALTDYQFMRPRAKDKKLIEEWAVPLKSIEDIY 240
PKSYISRT GW +PHGRW DS + +Y L+DYQF RP+ +DKKL +EW VPLKSIED+
Sbjct: 336 PKSYISRTKGWNDFPHGRWGDSHSAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQ 395
Query: 241 ERFRLFCLGKLRTNPWSELDGLQPETKIINEHLEKINTKGFLTINSQPAVNGEKSDSPIV 300
E+F+ C+G L+++PWSELDGLQPETKIINE L KIN+ GFLTINSQP+VN KSDSP +
Sbjct: 396 EKFKELCIGNLKSSPWSELDGLQPETKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAI 455
Query: 301 GWGGPGGYIYQKAYLEFFCSKEKLDALIEKSKDRPFLTYMAVNKEGIWKSNVGQTDVNAV 360
GWGGPGGY+YQKAYLEFFCSK+KLD L+EKSK P +TYMAVNK W SN G++DVNAV
Sbjct: 456 GWGGPGGYVYQKAYLEFFCSKDKLDTLVEKSKAFPSITYMAVNKSENWVSNTGESDVNAV 515
Query: 361 TWGVFPAKEIIQPTIVDPVSFNVWKDEAFEIWSRGWASLYPEADASRKLVEEVGSSYFLV 420
TWGVFPAKE+IQPTIVDP SF VWKDEAFEIWSR WA+LYPE D SRKL+EEV +SY+LV
Sbjct: 516 TWGVFPAKEVIQPTIVDPASFKVWKDEAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLV 575
Query: 421 SLVDNDYANGDLF 433
SLVDN+Y NGD+F
Sbjct: 576 SLVDNNYINGDIF 588
>At3g13490 lysyl-tRNA synthetase like protein
Length = 602
Score = 33.5 bits (75), Expect = 0.24
Identities = 20/71 (28%), Positives = 30/71 (42%)
Query: 226 IEEWAVPLKSIEDIYERFRLFCLGKLRTNPWSELDGLQPETKIINEHLEKINTKGFLTIN 285
I A P + + ERF LF G+ N +SEL + + E + + N K +
Sbjct: 474 ISPLAKPHRGNAGLTERFELFICGREMANAFSELTDPVDQRTRLEEQVRQHNAKRAEAVR 533
Query: 286 SQPAVNGEKSD 296
P N +K D
Sbjct: 534 ESPEPNAKKDD 544
>At2g42270 ATP-dependent RNA helicase like protein
Length = 2172
Score = 31.6 bits (70), Expect = 0.90
Identities = 18/63 (28%), Positives = 31/63 (48%)
Query: 100 NEEAVKAYGIHLGTEMCKKIMAHGIKTVHLYTLNMEKSALAILTSLGLIEESKISRSLPW 159
N E ++ + I + E K+ + HGI +H N+++ + L G I+ +S SL W
Sbjct: 1621 NLEELEPFLIQICEETLKETLRHGIGYLHEGLSNLDQEIVTQLFEAGRIQVCVMSSSLCW 1680
Query: 160 RRP 162
P
Sbjct: 1681 GTP 1683
>At4g07590
Length = 860
Score = 30.8 bits (68), Expect = 1.5
Identities = 26/87 (29%), Positives = 41/87 (46%), Gaps = 4/87 (4%)
Query: 64 PGIMPINNYKGFIRMTGFCKTKIP-AEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAH 122
P I+P Y FC T+ P + +A L ++D + K+ HL T M +A+
Sbjct: 343 PDILPAEEYIRLFMQNEFCSTRYPCSTTLAQLGLLEDVQHLYKS--CHLDTLMAYPYVAY 400
Query: 123 GIKTV-HLYTLNMEKSALAILTSLGLI 148
+T+ L TL +E +I +LG I
Sbjct: 401 EEETIKFLSTLEVELYQGSINQALGRI 427
>At1g63220 putative elicitor-responsive gene
Length = 147
Score = 30.4 bits (67), Expect = 2.0
Identities = 23/71 (32%), Positives = 34/71 (47%), Gaps = 5/71 (7%)
Query: 344 KEGIWKSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFNVWKDEAF--EIWSRGWASLYP 401
K I+ +VG D +AV P + + + P ++NV KDE + EIW S P
Sbjct: 68 KAKIFDKDVGTED-DAVGEATIPLEPVFVEGSIPPTAYNVVKDEEYKGEIWVA--LSFKP 124
Query: 402 EADASRKLVEE 412
+ SR + EE
Sbjct: 125 SENRSRGMDEE 135
>At1g42360 hypothetical protein
Length = 335
Score = 30.4 bits (67), Expect = 2.0
Identities = 20/74 (27%), Positives = 37/74 (49%), Gaps = 4/74 (5%)
Query: 64 PGIMPINNYKGFIRMTGFCKTKIPAEI-MAALEPIKDNEEAVKAYGIHLGTEMCKKIMAH 122
P I+P Y ++ FC T+ P +A L ++D + ++ HL T M +A+
Sbjct: 79 PDILPTEEYVRLFKLNEFCSTRYPCSTSLAQLGLLEDVQHLYQS--CHLDTLMAYPYVAY 136
Query: 123 GIKTVH-LYTLNME 135
+T+ L+TL ++
Sbjct: 137 EDETIQFLFTLQVK 150
>At2g06330 putative Athila retroelement ORF1 protein
Length = 555
Score = 29.3 bits (64), Expect = 4.5
Identities = 19/74 (25%), Positives = 37/74 (49%), Gaps = 4/74 (5%)
Query: 64 PGIMPINNYKGFIRMTGFCKTKIP-AEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAH 122
P ++P Y ++ FC T+ P + +A L ++D + ++ HL T M +A+
Sbjct: 64 PDVLPAEEYIRLFKLNEFCSTRYPCSTTLAQLRLLEDVQHLYQS--CHLDTLMAYPYVAY 121
Query: 123 GIKTVH-LYTLNME 135
+T+ L T+ +E
Sbjct: 122 EEETIQFLSTMQVE 135
>At5g07480 putative protein
Length = 356
Score = 28.9 bits (63), Expect = 5.8
Identities = 18/53 (33%), Positives = 33/53 (61%), Gaps = 6/53 (11%)
Query: 109 IHLGTEMCKKIMAHGI--KTVHLYTLNMEKS--ALAILTSLGLIEESKISRSL 157
+H+G + +++++G+ VH TLN EK+ +LA L SLG+ ++ + R L
Sbjct: 266 VHIGDHV--EVLSNGLYKSIVHKVTLNEEKTRISLASLHSLGMDDKMSVPREL 316
>At1g42370 hypothetical protein
Length = 619
Score = 28.5 bits (62), Expect = 7.6
Identities = 20/74 (27%), Positives = 36/74 (48%), Gaps = 4/74 (5%)
Query: 64 PGIMPINNYKGFIRMTGFCKTKIPAEI-MAALEPIKDNEEAVKAYGIHLGTEMCKKIMAH 122
P I+P Y ++ FC T+ P +A L ++D + ++ HL T M +A+
Sbjct: 79 PDILPTEEYVRLFKLNEFCSTRYPCSTSLAQLGLLEDVQHLYQS--CHLDTLMAYPYVAY 136
Query: 123 GIKTVH-LYTLNME 135
+T+ L TL ++
Sbjct: 137 EDETIQFLSTLQVK 150
>At1g02940
Length = 256
Score = 28.1 bits (61), Expect = 9.9
Identities = 15/43 (34%), Positives = 19/43 (43%), Gaps = 10/43 (23%)
Query: 199 WSDSGNPSYGALTDYQFMRPRAKDKKLIEEWAVPLKSIEDIYE 241
W S P YG TDY K++ E L+ + DIYE
Sbjct: 153 WEQSIKPMYGLKTDY----------KVVNETEAKLEKVLDIYE 185
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.138 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,429,362
Number of Sequences: 26719
Number of extensions: 465955
Number of successful extensions: 928
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 924
Number of HSP's gapped (non-prelim): 11
length of query: 436
length of database: 11,318,596
effective HSP length: 102
effective length of query: 334
effective length of database: 8,593,258
effective search space: 2870148172
effective search space used: 2870148172
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC136287.1