BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136286.9 - phase: 0
(222 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53070 unknown protein (At5g53070) 237 4e-63
At3g44890 RP19 gene for chloroplast ribosomal protein CL9 37 0.006
At5g64870 nodulin-like 30 1.3
At5g32440 unknown protein 30 1.3
At5g04890 unknown protein 30 1.3
At4g23390 unknown protein 29 2.2
At5g66670 At14a protein-like 28 2.9
At5g44290 cyclin-dependent protein kinase-like protein 28 2.9
At1g32750 unknown protein 28 2.9
At1g22420 28 2.9
At1g10300 putative GTP-binding protein 28 2.9
At1g04890 hypothetical protein 28 2.9
At2g24440 unknown protein 28 3.8
At5g62100 unknown protein 28 5.0
At4g32190 unknown protein 28 5.0
At4g12430 putative trehalose-6-phosphate phosphatase (AtTPPA) 28 5.0
At2g42250 putative cytochrome P450 28 5.0
At1g02970 unknown protein 28 5.0
At4g05150 unknown protein 27 6.5
At1g67050 unknown protein 27 6.5
>At5g53070 unknown protein (At5g53070)
Length = 221
Score = 237 bits (604), Expect = 4e-63
Identities = 127/222 (57%), Positives = 164/222 (73%), Gaps = 5/222 (2%)
Query: 1 MGYLQFGRYSVRQIVRFKDVVN--EGVVVNPLMYASQGLRYNRKLQVILTTNIDKLGKAG 58
M Y+ R +R +V + E + +PL++A QG+RY RKL+VILTT I+KLGKAG
Sbjct: 1 MAYVGQSRNVIRHVVSRGTAYHKYENAIHHPLLFACQGVRY-RKLEVILTTGIEKLGKAG 59
Query: 59 ETVKVAPGFFRNHLMPKLLAVPNIDKFAYLLTEQRKIYQPTEEVKQEDVVVVTESKEDLM 118
ETVKVAPG+FRNHLMPKLLAVPNIDK+AYL+ EQRK+Y EEVK+E V VV ++ E
Sbjct: 60 ETVKVAPGYFRNHLMPKLLAVPNIDKYAYLIREQRKMYNH-EEVKEE-VKVVHKTSEVQT 117
Query: 119 KEYERAALILDKAKLVLRRLIDVKKAKARESKDEPLELQIPVSKNALVAEVARQLCVNIT 178
KEYE+AA L A LVLR+L+D +K K R SKD+ ++Q P++K +V+EVARQLCV I
Sbjct: 118 KEYEKAAKRLANANLVLRKLVDKEKFKNRSSKDDKPDVQTPITKEEIVSEVARQLCVKID 177
Query: 179 AENLHLPTPLSTIGEYEVPLRLPRSIPLPEGKLNWALKVKIR 220
+N+ L PL T GEYEVPL+ P++IPLP+G + W LKVK+R
Sbjct: 178 PDNVVLAAPLETFGEYEVPLKFPKTIPLPQGTVQWILKVKVR 219
>At3g44890 RP19 gene for chloroplast ribosomal protein CL9
Length = 197
Score = 37.4 bits (85), Expect = 0.006
Identities = 42/159 (26%), Positives = 71/159 (44%), Gaps = 29/159 (18%)
Query: 44 QVILTTNIDKLGKAGETVKVAPGFFRNHLMPKLLAVPNIDKFAYLLTEQRKIYQP--TEE 101
+VIL ++ LGK G+ + V GFFRN L+P T + ++ P +E
Sbjct: 51 KVILKEDVTDLGKQGQLLDVKAGFFRNFLLP---------------TGKAQLMTPLLLKE 95
Query: 102 VKQEDVVVVTESKEDLMKEYERAALILDKAKLVLRRLIDVKKAKARESKDEPLELQIPVS 161
+K ED + E K+ + +E ++ A++ + + K K + K + + V+
Sbjct: 96 LKMEDERIEAE-KQRVKEEAQQLAMVF--------QTVGAFKVKRKGGKGKLIFGS--VT 144
Query: 162 KNALVAEVARQLCVNITAENLHLPTPLSTIGEYEVPLRL 200
LV + QL +I + LP T GEY L+L
Sbjct: 145 AQDLVDIIKSQLQKDIDKRLVSLPEIRET-GEYIAELKL 182
>At5g64870 nodulin-like
Length = 479
Score = 29.6 bits (65), Expect = 1.3
Identities = 28/86 (32%), Positives = 46/86 (52%), Gaps = 4/86 (4%)
Query: 82 IDKFAYLLTEQRKIYQPTEEVKQEDVVVVTESKEDLMKEYERAALILDKAKLVLR-RLID 140
ID + +++ QR EE+K + V V +++++ + AAL + KA L R+ +
Sbjct: 206 IDAESKIISTQRLGEGTKEEIKVKTEVKVFQNEKEALVAKADAALAIQKAALSQNSRVAE 265
Query: 141 VKKAKARESKDEPLELQIPVSK-NAL 165
V+ AKA ++ ELQ V K NAL
Sbjct: 266 VEAAKAVALRE--AELQTKVEKMNAL 289
>At5g32440 unknown protein
Length = 264
Score = 29.6 bits (65), Expect = 1.3
Identities = 21/71 (29%), Positives = 38/71 (52%), Gaps = 2/71 (2%)
Query: 114 KEDLMKEYERAALILDKAKLVLRRLIDVKKAKARESKDEPLELQIPVSKNALVAEVARQL 173
+E++M + + A++ + + +L+R + ++ + RES+D+ ELQ E R L
Sbjct: 178 QENMMLKQQLEAIVQENS--LLKRAVVTQQKRQRESEDQSQELQHLRQLVTQYQEQLRTL 235
Query: 174 CVNITAENLHL 184
VN A LHL
Sbjct: 236 EVNNYALTLHL 246
>At5g04890 unknown protein
Length = 366
Score = 29.6 bits (65), Expect = 1.3
Identities = 23/87 (26%), Positives = 43/87 (48%), Gaps = 7/87 (8%)
Query: 66 GFFRNHLMPKLLAVPNIDKFAYLLTEQRKIYQPTEEVKQEDVVVVTESKEDLMKEYERAA 125
G F+N+++ + I K AYL R TE E + E + L++E R
Sbjct: 90 GSFKNNVLTITMPKETITKVAYLPETSR-----TEAAALEKAAKLEEKR--LLEESRRKE 142
Query: 126 LILDKAKLVLRRLIDVKKAKARESKDE 152
++AK + ++L++ K+A R+ ++E
Sbjct: 143 KEEEEAKQMKKQLLEEKEALIRKLQEE 169
>At4g23390 unknown protein
Length = 401
Score = 28.9 bits (63), Expect = 2.2
Identities = 21/68 (30%), Positives = 29/68 (41%)
Query: 37 LRYNRKLQVILTTNIDKLGKAGETVKVAPGFFRNHLMPKLLAVPNIDKFAYLLTEQRKIY 96
+RY+ KL ++LT + L GE+ K P M KLL N TE I+
Sbjct: 1 MRYSNKLIILLTLSTIALILVGESHKTIPSKEEKEEMKKLLTYLNKPAVKSFQTEHGYIF 60
Query: 97 QPTEEVKQ 104
+ KQ
Sbjct: 61 DCIDIQKQ 68
>At5g66670 At14a protein-like
Length = 408
Score = 28.5 bits (62), Expect = 2.9
Identities = 13/34 (38%), Positives = 22/34 (64%)
Query: 94 KIYQPTEEVKQEDVVVVTESKEDLMKEYERAALI 127
++Y E+ Q+ V V+ ESKED++K + AL+
Sbjct: 89 EVYGYLLELNQDTVRVIIESKEDVLKNNDLKALV 122
>At5g44290 cyclin-dependent protein kinase-like protein
Length = 644
Score = 28.5 bits (62), Expect = 2.9
Identities = 14/30 (46%), Positives = 19/30 (62%)
Query: 96 YQPTEEVKQEDVVVVTESKEDLMKEYERAA 125
+ P EE+K+E VV+ S E + KE E AA
Sbjct: 83 HHPPEELKREPSVVIPPSPETVSKEAELAA 112
>At1g32750 unknown protein
Length = 1919
Score = 28.5 bits (62), Expect = 2.9
Identities = 18/40 (45%), Positives = 22/40 (55%), Gaps = 4/40 (10%)
Query: 91 EQRKIYQPTEEVKQEDVVVVTESKEDLMKEYERAALILDK 130
EQ + + E K+E VV+ KED EYE A ILDK
Sbjct: 156 EQAPVEKSLETEKREPVVL----KEDKALEYEEEASILDK 191
>At1g22420
Length = 480
Score = 28.5 bits (62), Expect = 2.9
Identities = 24/79 (30%), Positives = 36/79 (45%), Gaps = 11/79 (13%)
Query: 149 SKDEPLELQIPVSKNALVAEVARQLCVNITAEN-----LHLPTPLSTIGEYEVPLRLPRS 203
S DE L Q+P S N ++ V + EN + LP PL+ I VP ++ +
Sbjct: 245 SPDESLYWQLPESLNGPLSPSLMLSPVQRSPENSLLGSVDLPNPLNNIRPENVPPQVSAA 304
Query: 204 IPLPEGKLNWALKVKIRSK 222
PL W+L I+S+
Sbjct: 305 SPL------WSLDCPIQSE 317
>At1g10300 putative GTP-binding protein
Length = 687
Score = 28.5 bits (62), Expect = 2.9
Identities = 20/60 (33%), Positives = 33/60 (54%), Gaps = 7/60 (11%)
Query: 98 PTEEVKQEDVVVVTESKEDLMKEYERA---ALILDKAKL----VLRRLIDVKKAKARESK 150
P E + +ED ++ E K++ MK A A+IL+ + L V+ RL+D + A +SK
Sbjct: 324 PMENLSEEDRKLIEEMKDEAMKTEMGASEEAVILEMSTLTEEGVMSRLLDQRVAAKMKSK 383
>At1g04890 hypothetical protein
Length = 494
Score = 28.5 bits (62), Expect = 2.9
Identities = 35/167 (20%), Positives = 70/167 (40%), Gaps = 9/167 (5%)
Query: 55 GKAGETVKVA--PGFFRNHLMPKLLAVPNIDKFAYLLTEQRKIYQPTEEVKQEDVVVVTE 112
GKAGE ++ P F + + + + + + +++ +++ + EE+ +E+
Sbjct: 154 GKAGEGTNLSHWPEFEKTVSVNQQVNSNSSAEQSFVRNVEKRSVRDLEELLKEERAARAT 213
Query: 113 SKEDLMKEYERAALILDKAKLVLRRLIDVKKAKARESKDEPLELQIPVSKNALVAEVARQ 172
+L KE AA D+A ++ RL D K A E++ ++ + +A + +
Sbjct: 214 VCVELDKERSAAASAADEAMAMIHRLQDEKAAIEMEARQFQRLVEERSTFDAEEMVILKD 273
Query: 173 LCVNITAENLHLPTP-------LSTIGEYEVPLRLPRSIPLPEGKLN 212
+ + E L L E E L +++P PE K N
Sbjct: 274 ILIRREREKHFLEKEVEAYRQLLEETEELECSLIKEKNVPEPEHKQN 320
>At2g24440 unknown protein
Length = 183
Score = 28.1 bits (61), Expect = 3.8
Identities = 28/112 (25%), Positives = 51/112 (45%), Gaps = 9/112 (8%)
Query: 75 KLLAVPNIDKFAYLLTEQRKIYQPTEEVKQEDVVVVTESKED-----LMKEYERAALILD 129
KL+ + + +K T+ + + ++VK+E+V V E +E+ KE + + D
Sbjct: 40 KLMKIESPEKKKRKTTKAKNVGAAKKKVKKEEVAVKIEKEEEEDDDAAEKEEDDDS---D 96
Query: 130 KAKLVLRRLIDVKKAKARESK-DEPLELQIPVSKNALVAEVARQLCVNITAE 180
K K+V+ K K R ++ E LE +P + + R+ C I E
Sbjct: 97 KKKIVIEHCKQCKSFKERANEVKEGLEEAVPGIIVTVNPDKPRRGCFEIREE 148
>At5g62100 unknown protein
Length = 296
Score = 27.7 bits (60), Expect = 5.0
Identities = 16/72 (22%), Positives = 36/72 (49%), Gaps = 8/72 (11%)
Query: 91 EQRKIYQPTEEVKQEDVVVVTESKEDLMKEYERAALILDKAKLVL--------RRLIDVK 142
E +KI V +D+ ++ + KE K + + + D++KL+L +RL++++
Sbjct: 62 ELKKILSGATGVHHQDMQIIYKDKERDSKMFLDLSGVKDRSKLILIEDPISQEKRLLELR 121
Query: 143 KAKARESKDEPL 154
K +E + +
Sbjct: 122 KIATKEKSSKAI 133
>At4g32190 unknown protein
Length = 783
Score = 27.7 bits (60), Expect = 5.0
Identities = 25/94 (26%), Positives = 44/94 (46%), Gaps = 7/94 (7%)
Query: 91 EQRKIYQPTEEVKQEDVV-----VVTESKEDLM--KEYERAALILDKAKLVLRRLIDVKK 143
E+R Q + +K+E++ + SKE M E+E + +L KA V++R
Sbjct: 196 EERAALQSSLTLKEEELEKMRQEIANRSKEVSMAISEFESKSQLLSKANEVVKRQEGEIY 255
Query: 144 AKARESKDEPLELQIPVSKNALVAEVARQLCVNI 177
A R +++ EL+I + L E R+ N+
Sbjct: 256 ALQRALEEKEEELEISKATKKLEQEKLRETEANL 289
>At4g12430 putative trehalose-6-phosphate phosphatase (AtTPPA)
Length = 368
Score = 27.7 bits (60), Expect = 5.0
Identities = 17/55 (30%), Positives = 28/55 (50%)
Query: 102 VKQEDVVVVTESKEDLMKEYERAALILDKAKLVLRRLIDVKKAKARESKDEPLEL 156
V ++D ++ + D +K+Y R L + L +R +ID K +A E E L L
Sbjct: 240 VDEKDWPIIAQRVHDHLKQYPRLRLTHGRKVLEVRPVIDWNKGRAVEFLLESLGL 294
>At2g42250 putative cytochrome P450
Length = 514
Score = 27.7 bits (60), Expect = 5.0
Identities = 12/27 (44%), Positives = 18/27 (66%)
Query: 67 FFRNHLMPKLLAVPNIDKFAYLLTEQR 93
F + M KLLAVP ++KFA + E++
Sbjct: 135 FMKKLCMTKLLAVPQLEKFADIREEEK 161
>At1g02970 unknown protein
Length = 500
Score = 27.7 bits (60), Expect = 5.0
Identities = 14/68 (20%), Positives = 34/68 (49%)
Query: 142 KKAKARESKDEPLELQIPVSKNALVAEVARQLCVNITAENLHLPTPLSTIGEYEVPLRLP 201
KK+ + S+ E L + ++K +++ +N+++ + +G++ RL
Sbjct: 338 KKSSLKVSEREILVIMHQIAKALHFVHEKGIAHLDVKPDNIYIKNGVCKLGDFGCATRLD 397
Query: 202 RSIPLPEG 209
+S+P+ EG
Sbjct: 398 KSLPVEEG 405
>At4g05150 unknown protein
Length = 477
Score = 27.3 bits (59), Expect = 6.5
Identities = 24/87 (27%), Positives = 37/87 (41%), Gaps = 10/87 (11%)
Query: 75 KLLAVPNIDKFAYLLTEQRKI---------YQ-PTEEVKQEDVVVVTESKEDLMKEYERA 124
+++AV FA LL++ K+ YQ P E++ V E E++M EY+R
Sbjct: 86 RMVAVHRHTTFASLLSKLAKLSGKSNISVKYQLPNEDLDALISVSTDEDVENMMDEYDRV 145
Query: 125 ALILDKAKLVLRRLIDVKKAKARESKD 151
A + LR + K E D
Sbjct: 146 AQNQNPRASRLRLFLFTKNVAGEEDND 172
>At1g67050 unknown protein
Length = 264
Score = 27.3 bits (59), Expect = 6.5
Identities = 12/24 (50%), Positives = 18/24 (75%), Gaps = 2/24 (8%)
Query: 91 EQRKIYQPTEEVKQEDVVVVTESK 114
+QRK QP EE +++DV++ TE K
Sbjct: 110 KQRK--QPNEEQQEDDVIITTEEK 131
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.318 0.137 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,607,409
Number of Sequences: 26719
Number of extensions: 186808
Number of successful extensions: 599
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 588
Number of HSP's gapped (non-prelim): 27
length of query: 222
length of database: 11,318,596
effective HSP length: 96
effective length of query: 126
effective length of database: 8,753,572
effective search space: 1102950072
effective search space used: 1102950072
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC136286.9