BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136286.14 - phase: 0
(159 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g14870 putative protein 113 5e-26
At1g62740 TPR-repeat protein 35 0.017
At1g49490 hypothetical protein 35 0.022
At5g58160 strong similarity to unknown protein (gb|AAD23008.1) 33 0.065
At4g21330 putative protein 33 0.065
At2g28440 En/Spm-like transposon protein 33 0.065
At1g80070 hypothetical protein 33 0.065
At2g24050 putative eukaryotic initiation factor 4, eIF4 32 0.11
At1g12270 putative protein 32 0.14
At4g00890 32 0.19
At1g08520 Mg-chelatase like protein 31 0.25
At1g62440 putative extensin-like protein (gnl|PID|e1310400 31 0.32
At4g37120 step II splicing factor like protein 30 0.42
At4g15070 hypothetical protein 30 0.42
At3g24400 protein kinase, putative 30 0.42
At2g24060 putative chloroplast initiation factor 3 30 0.42
At3g58600 unknown protein 30 0.55
At1g62760 hypothetical protein 30 0.55
At1g44030 hypothetical protein 30 0.55
At1g20290 hypothetical protein 30 0.55
>At4g14870 putative protein
Length = 177
Score = 113 bits (282), Expect = 5e-26
Identities = 65/128 (50%), Positives = 85/128 (65%), Gaps = 15/128 (11%)
Query: 47 CTLPRAVEEK-DQPTSNPEPEPEPKP----------ELNESELASELKKAMQERK---EQ 92
C + +A+E++ D S E E P P E+ S + +E+K AM++RK E+
Sbjct: 50 CFVAKAIEQRRDTAGSESESEATPSPAEESGSGEDKEVEISAIGAEIKAAMEQRKTAEEE 109
Query: 93 EGNN-FWNGVVSEIGEIEWPEFGKVLGTTGVVLSVIFGSSAVLLTLNAILAELSDKVFAG 151
+G N F +GV E+ EIEWP F KVLGTTGVVL VI GSS VLLT+N +LAELSD+VF G
Sbjct: 110 KGKNEFLSGVAEEVKEIEWPAFQKVLGTTGVVLGVIAGSSVVLLTVNFLLAELSDRVFIG 169
Query: 152 KGVQDFFT 159
+GVQDFF+
Sbjct: 170 RGVQDFFS 177
>At1g62740 TPR-repeat protein
Length = 571
Score = 35.0 bits (79), Expect = 0.017
Identities = 18/48 (37%), Positives = 27/48 (55%), Gaps = 5/48 (10%)
Query: 49 LPRAVEEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQEGNN 96
+P E + + PEPEPEP+PE E E +K ++ +KE+E N
Sbjct: 209 VPSRKEPEVEKKRKPEPEPEPEPEFGE-----EKQKKLKAQKEKELGN 251
>At1g49490 hypothetical protein
Length = 847
Score = 34.7 bits (78), Expect = 0.022
Identities = 22/77 (28%), Positives = 33/77 (42%), Gaps = 9/77 (11%)
Query: 18 TPSPPSTLSPQFFNFKLKPSLPSLTLTRRC--TLPRAVEEKDQPTSNPEPEPEPKPELNE 75
+P+PP T P KPS P + + + P + +QP+ P+P P+ E
Sbjct: 395 SPNPPRTSEP-------KPSKPEPVMPKPSDSSKPETPKTPEQPSPKPQPPKHESPKPEE 447
Query: 76 SELASELKKAMQERKEQ 92
E EL K + K Q
Sbjct: 448 PENKHELPKQKESPKPQ 464
Score = 28.5 bits (62), Expect = 1.6
Identities = 19/59 (32%), Positives = 26/59 (43%), Gaps = 4/59 (6%)
Query: 15 PHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPT-SNPEPEPEPKPE 72
P P PP SP+ + K LP + P+ + +D P P+PE PKPE
Sbjct: 431 PSPKPQPPKHESPKPEEPENKHELPK---QKESPKPQPSKPEDSPKPEQPKPEESPKPE 486
>At5g58160 strong similarity to unknown protein (gb|AAD23008.1)
Length = 1307
Score = 33.1 bits (74), Expect = 0.065
Identities = 22/76 (28%), Positives = 34/76 (43%), Gaps = 9/76 (11%)
Query: 5 AKTSFTLTLSPHFTPSPPS----TLSPQFFNFKLKPSL-----PSLTLTRRCTLPRAVEE 55
+KT+ +L LSP +P+ P+ T+S FF P L + L + P +
Sbjct: 620 SKTTNSLLLSPQASPATPTNPSKTVSVDFFGAATSPHLGASDNVASNLGQPARSPPPISN 679
Query: 56 KDQPTSNPEPEPEPKP 71
D+ + P P P P P
Sbjct: 680 SDKKPALPRPPPPPPP 695
>At4g21330 putative protein
Length = 207
Score = 33.1 bits (74), Expect = 0.065
Identities = 26/101 (25%), Positives = 47/101 (45%), Gaps = 7/101 (6%)
Query: 53 VEEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQ----EGNNFWNGVVSEIGEI 108
+EE + +P KPE+ S+L E+KK E Q FW +++E +
Sbjct: 91 MEEAPPEIDEEQTDPMIKPEVETSDLNEEMKKLGIEENVQLCKIGERKFWLKIITEKRDG 150
Query: 109 EWPEFGKV---LGTTGVVLSVIFGSSAVLLTLNAILAELSD 146
+ +F +V LG + +S+ + A+L++ + EL D
Sbjct: 151 IFTKFMEVMRFLGFEIIDISLTTSNGAILISASVQTQELCD 191
>At2g28440 En/Spm-like transposon protein
Length = 268
Score = 33.1 bits (74), Expect = 0.065
Identities = 30/124 (24%), Positives = 45/124 (36%), Gaps = 23/124 (18%)
Query: 10 TLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPE--- 66
+L SP P PP SP PS P P D +PEPE
Sbjct: 139 SLADSPSPPPPPPQPESPS------SPSYPE-------PAPVPAPSDDDSDDDPEPETEY 185
Query: 67 ---PEPKPELNESELASELKKAMQERKEQEGNNFWNGVVSEIGEIEWPEFGKVLGTTGVV 123
P P PEL ++ A +E ++ G ++ + + G G +LG +V
Sbjct: 186 FPSPAPSPELGMAQDIKASDAAGEELNDERGEDYGMSGLEKAG----IAIGTILGVGAIV 241
Query: 124 LSVI 127
+ +
Sbjct: 242 IGAL 245
>At1g80070 hypothetical protein
Length = 2382
Score = 33.1 bits (74), Expect = 0.065
Identities = 20/54 (37%), Positives = 27/54 (49%)
Query: 59 PTSNPEPEPEPKPELNESELASELKKAMQERKEQEGNNFWNGVVSEIGEIEWPE 112
P SNP P EP PE E++L + +K MQ ++ G+ G V E PE
Sbjct: 35 PPSNPTPPVEPTPEEAEAKLEEKARKWMQLNSKRYGDKRKFGFVETQKEDMPPE 88
>At2g24050 putative eukaryotic initiation factor 4, eIF4
Length = 747
Score = 32.3 bits (72), Expect = 0.11
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 3/42 (7%)
Query: 50 PRAVEEKDQPTSNPEPEPEPKP---ELNESELASELKKAMQE 88
P EK QP S P+P+P+ P LN EL + K ++E
Sbjct: 552 PIPTVEKPQPRSQPQPQPQAAPLANSLNAGELERKTKSLLEE 593
Score = 26.9 bits (58), Expect = 4.7
Identities = 28/89 (31%), Positives = 37/89 (41%), Gaps = 16/89 (17%)
Query: 15 PHFTPSPP--------STLSPQ----FFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPT-S 61
P F P P S L PQ N +PS P L + P+A K PT
Sbjct: 501 PRFQPPPAINKSLSVNSRLLPQGSGGLLNGGGRPS-PLLQGNGSSSAPQA--SKPIPTVE 557
Query: 62 NPEPEPEPKPELNESELASELKKAMQERK 90
P+P +P+P+ + LA+ L ERK
Sbjct: 558 KPQPRSQPQPQPQAAPLANSLNAGELERK 586
>At1g12270 putative protein
Length = 572
Score = 32.0 bits (71), Expect = 0.14
Identities = 13/28 (46%), Positives = 19/28 (67%)
Query: 64 EPEPEPKPELNESELASELKKAMQERKE 91
EPEPEP+PE+ E + E K+ ++ KE
Sbjct: 222 EPEPEPEPEVTEEKEKKERKEKAKKEKE 249
Score = 27.3 bits (59), Expect = 3.6
Identities = 16/66 (24%), Positives = 28/66 (42%)
Query: 31 NFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELNESELASELKKAMQERK 90
N K +P P ++P + EPEP+PE +E + ++ + +K
Sbjct: 187 NVKFRPPPPQGDEAEVPESDMGQSSSNEPEVEKKREPEPEPEPEVTEEKEKKERKEKAKK 246
Query: 91 EQEGNN 96
E+E N
Sbjct: 247 EKELGN 252
>At4g00890
Length = 431
Score = 31.6 bits (70), Expect = 0.19
Identities = 23/74 (31%), Positives = 34/74 (45%), Gaps = 12/74 (16%)
Query: 16 HFTPSPPSTLSPQFF-NFKLKPSLPSLTLTRRCTL---------PRAVEEKDQPTSNP-- 63
H +PSPP LSP+ N + K +P + T + +L P + P S+P
Sbjct: 261 HSSPSPPPLLSPKASENHQSKSPMPPPSPTAQISLSSLKSPIPSPATITAPPPPFSSPLS 320
Query: 64 EPEPEPKPELNESE 77
+ P PKP L + E
Sbjct: 321 QTTPSPKPSLPQIE 334
>At1g08520 Mg-chelatase like protein
Length = 760
Score = 31.2 bits (69), Expect = 0.25
Identities = 14/47 (29%), Positives = 22/47 (46%)
Query: 49 LPRAVEEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQEGN 95
LPR+ ++ P +P P P P N E ++ +E +E E N
Sbjct: 395 LPRSSLDETPPEQQNQPPPPPPPPQNSESGEEENEEEQEEEEEDESN 441
>At1g62440 putative extensin-like protein (gnl|PID|e1310400
Length = 786
Score = 30.8 bits (68), Expect = 0.32
Identities = 27/81 (33%), Positives = 40/81 (49%), Gaps = 13/81 (16%)
Query: 13 LSPHF--TPSPPST-LSPQFFNFKLKPSLPS--LTLTRRCTLP-----RAVEEKDQPTSN 62
+SP F TP PPS+ +SP +F+ P PS ++ + R T P + K P
Sbjct: 408 MSPTFRATPPPPSSKMSP---SFRATPPPPSSKMSPSFRATPPPPSSKMSPSVKAYPPPP 464
Query: 63 PEPEPEPKPELNESELASELK 83
P PE EP P SE++ ++
Sbjct: 465 PPPEYEPSPPPPSSEMSPSVR 485
>At4g37120 step II splicing factor like protein
Length = 536
Score = 30.4 bits (67), Expect = 0.42
Identities = 25/62 (40%), Positives = 30/62 (48%), Gaps = 9/62 (14%)
Query: 50 PRAVEEKDQPTSNPEPEPEPKPELNESELASELKK-------AMQERKEQEGNNFWNGVV 102
P+ VEEK T + PE ELNE LA+ LKK ERK + N+ N V
Sbjct: 456 PKKVEEKKMATWGTDI-PEDL-ELNEEALANALKKEDLSRREEKDERKRKYNVNYTNDVT 513
Query: 103 SE 104
SE
Sbjct: 514 SE 515
>At4g15070 hypothetical protein
Length = 889
Score = 30.4 bits (67), Expect = 0.42
Identities = 23/81 (28%), Positives = 34/81 (41%), Gaps = 10/81 (12%)
Query: 2 VLQAKTSFTLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTS 61
+L + SF+LT P +S F + S+P L E + +P S
Sbjct: 123 MLLIQESFSLTPEPELISLINQIISHVVFRQRQSESMPDLE----------AELEPEPDS 172
Query: 62 NPEPEPEPKPELNESELASEL 82
PE EPE E+N L ++L
Sbjct: 173 EPESEPEIDLEMNLISLITQL 193
>At3g24400 protein kinase, putative
Length = 694
Score = 30.4 bits (67), Expect = 0.42
Identities = 23/64 (35%), Positives = 26/64 (39%), Gaps = 7/64 (10%)
Query: 15 PHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELN 74
P TPSPP L+P P+ PS LT T P T +P P P P L
Sbjct: 68 PPSTPSPPPPLTPS--PLPPSPTTPSPPLTPSPTTP-----SPPLTPSPPPAITPSPPLT 120
Query: 75 ESEL 78
S L
Sbjct: 121 PSPL 124
Score = 29.6 bits (65), Expect = 0.72
Identities = 21/60 (35%), Positives = 23/60 (38%), Gaps = 1/60 (1%)
Query: 18 TPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPE-PKPELNES 76
TPSPP T SP + L PS P P + S P P P P P L S
Sbjct: 89 TPSPPLTPSPTTPSPPLTPSPPPAITPSPPLTPSPLPPSPTTPSPPPPSPSIPSPPLTPS 148
>At2g24060 putative chloroplast initiation factor 3
Length = 312
Score = 30.4 bits (67), Expect = 0.42
Identities = 14/38 (36%), Positives = 19/38 (49%)
Query: 35 KPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPE 72
KP P ++ A + T+ PEPEPEP+PE
Sbjct: 257 KPQEPPTRKKKKTAENEASASAAEITAEPEPEPEPEPE 294
Score = 30.4 bits (67), Expect = 0.42
Identities = 11/19 (57%), Positives = 14/19 (72%)
Query: 54 EEKDQPTSNPEPEPEPKPE 72
E + +P PEPEPEP+PE
Sbjct: 284 EPEPEPEPEPEPEPEPEPE 302
Score = 30.4 bits (67), Expect = 0.42
Identities = 11/19 (57%), Positives = 14/19 (72%)
Query: 54 EEKDQPTSNPEPEPEPKPE 72
E + +P PEPEPEP+PE
Sbjct: 288 EPEPEPEPEPEPEPEPEPE 306
Score = 30.4 bits (67), Expect = 0.42
Identities = 11/19 (57%), Positives = 14/19 (72%)
Query: 54 EEKDQPTSNPEPEPEPKPE 72
E + +P PEPEPEP+PE
Sbjct: 286 EPEPEPEPEPEPEPEPEPE 304
Score = 30.0 bits (66), Expect = 0.55
Identities = 12/21 (57%), Positives = 14/21 (66%)
Query: 52 AVEEKDQPTSNPEPEPEPKPE 72
A E +P PEPEPEP+PE
Sbjct: 278 AAEITAEPEPEPEPEPEPEPE 298
Score = 29.3 bits (64), Expect = 0.94
Identities = 10/20 (50%), Positives = 14/20 (70%)
Query: 53 VEEKDQPTSNPEPEPEPKPE 72
+ + +P PEPEPEP+PE
Sbjct: 281 ITAEPEPEPEPEPEPEPEPE 300
Score = 28.5 bits (62), Expect = 1.6
Identities = 10/18 (55%), Positives = 13/18 (71%)
Query: 54 EEKDQPTSNPEPEPEPKP 71
E + +P PEPEPEP+P
Sbjct: 290 EPEPEPEPEPEPEPEPEP 307
>At3g58600 unknown protein
Length = 302
Score = 30.0 bits (66), Expect = 0.55
Identities = 20/59 (33%), Positives = 26/59 (43%), Gaps = 5/59 (8%)
Query: 19 PSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELNESE 77
P PP LSP K SLP +L R+ E++D T E E E K +L +
Sbjct: 231 PPPPGPLSPVTTAQKSPSSLPP-----SLSLQRSSEQQDLDTKREEEEEEKKEDLKAKD 284
>At1g62760 hypothetical protein
Length = 312
Score = 30.0 bits (66), Expect = 0.55
Identities = 22/71 (30%), Positives = 32/71 (44%), Gaps = 3/71 (4%)
Query: 11 LTLSPHFTPSPPSTLSP-QFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEP 69
L+LSP P PP + SP + L PS PS + + P ++ P + P P
Sbjct: 61 LSLSPSSPPPPPPSSSPLSSLSPSLSPSPPSSSPS--SAPPSSLSPSSPPPLSLSPSSPP 118
Query: 70 KPELNESELAS 80
P + S L+S
Sbjct: 119 PPPPSSSPLSS 129
Score = 26.6 bits (57), Expect = 6.1
Identities = 18/58 (31%), Positives = 23/58 (39%), Gaps = 1/58 (1%)
Query: 14 SPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKP 71
SP +PSPPS+ L PS P L+ + P P S+ P P P
Sbjct: 33 SPSLSPSPPSSSPSSAPPSSLSPSSPP-PLSLSPSSPPPPPPSSSPLSSLSPSLSPSP 89
>At1g44030 hypothetical protein
Length = 597
Score = 30.0 bits (66), Expect = 0.55
Identities = 34/134 (25%), Positives = 55/134 (40%), Gaps = 33/134 (24%)
Query: 6 KTSFTLTLSPHFTPSPPSTLSPQFFN-FKLKPSLPSLTLTRRCTLPRAVEEKDQPTSN-- 62
K S ++ + P P F+ F L+P +P+ + DQ S
Sbjct: 56 KVSHLNSVGSDYEPKPDPEFMYMFYETFNLEP------------MPKLISTIDQIYSLFI 103
Query: 63 -PEPEPEPKPELN---ESELASELKK-----AMQERKEQEGNNFWNGVVSEIGEIEWPEF 113
P+PEP+PKPE++ ES+ S L + A++ E E G E P+F
Sbjct: 104 LPDPEPKPKPEMDPEQESKFISLLSQIYSIFAIKTNAELELRG---------GPEELPKF 154
Query: 114 GKVLGTTGVVLSVI 127
+ T ++S+I
Sbjct: 155 KSFISLTKQLMSLI 168
>At1g20290 hypothetical protein
Length = 497
Score = 30.0 bits (66), Expect = 0.55
Identities = 16/55 (29%), Positives = 28/55 (50%)
Query: 39 PSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELNESELASELKKAMQERKEQE 93
PS+ T+ +A EE+++ E E E + E E E E ++ +E +E+E
Sbjct: 350 PSMVHVGEGTIVQAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE 404
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.312 0.132 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,794,387
Number of Sequences: 26719
Number of extensions: 175777
Number of successful extensions: 1291
Number of sequences better than 10.0: 116
Number of HSP's better than 10.0 without gapping: 48
Number of HSP's successfully gapped in prelim test: 70
Number of HSP's that attempted gapping in prelim test: 1012
Number of HSP's gapped (non-prelim): 258
length of query: 159
length of database: 11,318,596
effective HSP length: 91
effective length of query: 68
effective length of database: 8,887,167
effective search space: 604327356
effective search space used: 604327356
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC136286.14