BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135795.5 + phase: 0
(471 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g05055 putative transcription factor 577 e-165
At4g38630 multiubiquitin chain binding protein (MBP1) 36 0.040
At5g63830 unknown protein 33 0.44
At3g11100 unknown protein 32 0.58
At4g27400 unknown protein (At4g27400) 30 3.8
At1g60200 30 3.8
At4g29530 unknown protein (At4g29530) 28 8.4
At4g03430 putative pre-mRNA splicing factor 28 8.4
At1g52980 unknown protein 28 8.4
>At1g05055 putative transcription factor
Length = 421
Score = 577 bits (1488), Expect = e-165
Identities = 273/360 (75%), Positives = 315/360 (86%), Gaps = 1/360 (0%)
Query: 14 EDDDEDEANDDGLEAWERAYTEDRSWESLQEDESGLLRPIDTTAIHHAQYRRRLRALASN 73
E ++ED+ + +G+ WERAY +DRSWE LQEDESGLLRPID +AI+HAQYRRRLR L++
Sbjct: 11 EREEEDDEDAEGIGEWERAYVDDRSWEELQEDESGLLRPIDNSAIYHAQYRRRLRMLSAA 70
Query: 74 AATARIQKGLIRYLYIVVDLSKAASERDFRPSRMAVIAKQVELFIREFFDQNPLSHVGLV 133
AA RIQKGLIRYLYIV+D S+AA+E DFRPSRMA++AK VE FIREFFDQNPLS +GLV
Sbjct: 71 AAGTRIQKGLIRYLYIVIDFSRAAAEMDFRPSRMAIMAKHVEAFIREFFDQNPLSQIGLV 130
Query: 134 TTKDGVANCLTDLGGSPESHIKALMGKLECSGDASLQNALELVHSNLNQIPSYGHREVLI 193
+ K+GVA+ LTDLGGSPE+HIKALMGKLE GD+SLQNALELVH +LNQ+PSYGHREVLI
Sbjct: 131 SIKNGVAHTLTDLGGSPETHIKALMGKLEALGDSSLQNALELVHEHLNQVPSYGHREVLI 190
Query: 194 LYSALSTCDPGDLMETIQKCKKSKIRCSVIGLAAEMFICKHLCQETGGTYSVALDESHFK 253
LYSAL TCDPGD+METIQKCKKSK+RCSVIGL+AEMFICKHLCQETGG YSVA+DE H K
Sbjct: 191 LYSALCTCDPGDIMETIQKCKKSKLRCSVIGLSAEMFICKHLCQETGGLYSVAVDEVHLK 250
Query: 254 ELILEHSPPPPAIAEYATANLIKMGFPQRAAEGSVAICTCHEEAKTGGGYTCPRCKVRVC 313
+L+LEH+PPPPAIAE+A ANLIKMGFPQRAAEGS+AIC+CH+E K G GY CPRCK RVC
Sbjct: 251 DLLLEHAPPPPAIAEFAIANLIKMGFPQRAAEGSMAICSCHKEVKIGAGYMCPRCKARVC 310
Query: 314 ELPTECRVCGLTLISSPHLARSYHHLFPIVPFAEI-SPSSQNDPNHSFPNTCFGCQQSLL 372
+LPTEC +CGLTL+SSPHLARSYHHLFPI PF E+ + SS ND +CFGCQQSL+
Sbjct: 311 DLPTECTICGLTLVSSPHLARSYHHLFPIAPFDEVPALSSLNDNRRKLGKSCFGCQQSLI 370
>At4g38630 multiubiquitin chain binding protein (MBP1)
Length = 386
Score = 36.2 bits (82), Expect = 0.040
Identities = 32/145 (22%), Positives = 61/145 (42%), Gaps = 10/145 (6%)
Query: 89 IVVDLSKAASERDFRPSRMAVIAKQVELFIREFFDQNPLSHVGLVTTKDGVANCLT---- 144
I +D S+ D+ PSR+ + V L NP + VG++T LT
Sbjct: 8 ICIDNSEWMRNGDYSPSRLQAQTEAVNLLCGAKTQSNPENTVGILTMAGKGVRVLTTPTS 67
Query: 145 DLGGSPESHIKALMGKLECSGDASLQNALELVHSNLNQIPSYGHREVLILYSALSTCDPG 204
DLG I A M L+ G+ +L A+++ L + R+ +I+++
Sbjct: 68 DLG-----KILACMHGLDVGGEINLTAAIQIAQLALKHRQNKNQRQRIIVFAGSPIKYEK 122
Query: 205 DLMETI-QKCKKSKIRCSVIGLAAE 228
+E + ++ KK+ + ++ +
Sbjct: 123 KALEIVGKRLKKNSVSLDIVNFGED 147
>At5g63830 unknown protein
Length = 406
Score = 32.7 bits (73), Expect = 0.44
Identities = 13/24 (54%), Positives = 13/24 (54%)
Query: 293 CHEEAKTGGGYTCPRCKVRVCELP 316
CH K YTCPRC R C LP
Sbjct: 27 CHVCNKQFSQYTCPRCNFRYCSLP 50
>At3g11100 unknown protein
Length = 249
Score = 32.3 bits (72), Expect = 0.58
Identities = 15/48 (31%), Positives = 22/48 (45%)
Query: 6 GKPLNGDLEDDDEDEANDDGLEAWERAYTEDRSWESLQEDESGLLRPI 53
G L+ D +DDD+DE +DD W + R E + E R +
Sbjct: 135 GSSLDDDDDDDDDDEEDDDDAGDWGFVVRKHRKVEDVDSSEGSAFREL 182
>At4g27400 unknown protein (At4g27400)
Length = 341
Score = 29.6 bits (65), Expect = 3.8
Identities = 15/36 (41%), Positives = 19/36 (52%), Gaps = 4/36 (11%)
Query: 288 VAICTCHEEAKTG----GGYTCPRCKVRVCELPTEC 319
VA C H +A G GG PRC++R + P EC
Sbjct: 15 VASCFLHVKAWHGQTYCGGNATPRCQLRYIDCPEEC 50
>At1g60200
Length = 781
Score = 29.6 bits (65), Expect = 3.8
Identities = 12/35 (34%), Positives = 20/35 (56%)
Query: 15 DDDEDEANDDGLEAWERAYTEDRSWESLQEDESGL 49
+++E+E +DD W RA ++R L+E E L
Sbjct: 373 EEEEEEDDDDSRRRWHRAALDERRRRQLREKEDDL 407
>At4g29530 unknown protein (At4g29530)
Length = 245
Score = 28.5 bits (62), Expect = 8.4
Identities = 32/120 (26%), Positives = 48/120 (39%), Gaps = 12/120 (10%)
Query: 206 LMETIQKCKKSKIRCSVIGLAAEMFICKHL-------CQETGGTYSVALDESHFKELILE 258
++E I+ K S ++ A + FI K L C T +LD++ ++
Sbjct: 76 IIEAIKSAKSSGCDLKIVSDANQFFIEKILEHHDLVDCFSEIYTNPTSLDDNGNLRILPY 135
Query: 259 HSPP-PPAIAEYATANLIKMGFPQ---RAAEGSVAICTCHEEAKTGGGYTCPRCKVRVCE 314
HS PP +NL K G RA+ + I GGG CP K+R C+
Sbjct: 136 HSDALPPHSCNLCPSNLCK-GLVMDHLRASSSNDQIPRRFIYLGDGGGDFCPTLKLRECD 194
>At4g03430 putative pre-mRNA splicing factor
Length = 1029
Score = 28.5 bits (62), Expect = 8.4
Identities = 14/45 (31%), Positives = 25/45 (55%), Gaps = 9/45 (20%)
Query: 5 AGKPLNGDLEDDDEDEANDDGLEAWERAYTEDRSWESLQEDESGL 49
AGKP + EDD+E E E+ Y E+++++ + ++ GL
Sbjct: 150 AGKPSEAEAEDDEEAE---------EKRYDENQTFDEFEGNDVGL 185
>At1g52980 unknown protein
Length = 576
Score = 28.5 bits (62), Expect = 8.4
Identities = 16/38 (42%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
Query: 10 NGDLEDDDEDEANDDGLEAWERAYTEDRSWESLQEDES 47
NG ++DED ++DG+E+ A ED E+ +EDES
Sbjct: 536 NGTDAEEDEDAVSEDGVESDSDA-DEDAVSENDEEDES 572
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.135 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,076,917
Number of Sequences: 26719
Number of extensions: 476321
Number of successful extensions: 2164
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 2137
Number of HSP's gapped (non-prelim): 19
length of query: 471
length of database: 11,318,596
effective HSP length: 103
effective length of query: 368
effective length of database: 8,566,539
effective search space: 3152486352
effective search space used: 3152486352
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)
Medicago: description of AC135795.5