BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135565.11 + phase: 0
(225 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g04890 unknown protein 214 4e-56
At2g46100 unknown protein 53 1e-07
At1g65230 unknown protein 39 0.002
At1g16320 unknown protein 39 0.002
At2g46215 unknown protein 38 0.005
At2g33210 mitochondrial chaperonin (HSP60) 38 0.005
At1g79510 unknown protein 35 0.042
At5g20140 unknown protein 32 0.35
At2g16270 unknown protein 30 1.0
At2g21070 unknown protein 30 1.3
At2g17630 putative phosphoserine aminotransferase 29 1.8
At1g78560 Unknown protein (T30F21.11) 29 2.3
At3g48060 putative protein 28 3.0
At3g56410 putative protein 28 3.9
At1g67490 alpha-glucosidase I 28 3.9
At4g31880 unknown protein 28 5.1
At3g49360 6-phosphogluconolactonase - like protein 27 6.7
At1g15660 unknown protein 27 6.7
>At3g04890 unknown protein
Length = 216
Score = 214 bits (544), Expect = 4e-56
Identities = 105/177 (59%), Positives = 137/177 (77%)
Query: 42 KNETPQVLKIAVSGVTELLRLFSPPQQTSVLSDDIEKQNNDSTVSSVEDVLIIIKSDYDN 101
+ +TP VLK AVSGVTE LRL S ++ ++ + ++ N+ T V+DV+ I++SDY N
Sbjct: 31 EKKTPAVLKWAVSGVTEFLRLISGAPSSTSIATNKDRSKNEVTAGDVDDVMGILRSDYRN 90
Query: 102 DYFVTGNFTSSIYTENCIFEDPTIKFSGRDLYARNLKLLVPFFDCASIKLLKIEKEVESD 161
YFVTG TS+IY+++CIFEDPTI F G +LY RNLKLLVPF + ASI+L +EK S
Sbjct: 91 FYFVTGVLTSAIYSDDCIFEDPTISFQGTELYERNLKLLVPFLEDASIELQNMEKSESSQ 150
Query: 162 TNFLRASWKLRTNLKLPWRPLIAIDGSTSYELNEDFKIVRHVESWNVSALEAVLQIF 218
N++ A+WKLRT LKLPWRPLI+I+G+T Y+L++DFKIVRHVESWNVSALEA+ QIF
Sbjct: 151 RNYILATWKLRTYLKLPWRPLISINGNTVYDLDKDFKIVRHVESWNVSALEAIGQIF 207
>At2g46100 unknown protein
Length = 286
Score = 53.1 bits (126), Expect = 1e-07
Identities = 29/74 (39%), Positives = 38/74 (51%), Gaps = 1/74 (1%)
Query: 95 IKSDYDNDYFVTGNFTSSIYTENCIFEDPTIKFSGRDLYARNLKLLVPFFDCASIKLLKI 154
IK D+ YFVTGN T +Y E C F DP F G + RN + +++KL+K
Sbjct: 109 IKQDFKRSYFVTGNLTPEVYEEKCEFADPAGSFKGLARFKRNCTNFGSLIEKSNMKLMKW 168
Query: 155 EK-EVESDTNFLRA 167
E EV S T R+
Sbjct: 169 ENFEVRSTTKRCRS 182
>At1g65230 unknown protein
Length = 286
Score = 38.9 bits (89), Expect = 0.002
Identities = 27/121 (22%), Positives = 55/121 (45%), Gaps = 18/121 (14%)
Query: 107 GNFTSSIYTENCIFEDPTIKFSGRDLYARNLKLLVPFFDCASIK--LLKIEKEVESDTNF 164
GN + Y + ++DP F+GR+ Y R P + + ++ + +++ V T+
Sbjct: 96 GNRAFACYAVSVKYKDPVRSFTGREKYKR------PMWITSGLENPTVTVQEMVMLSTSV 149
Query: 165 LRASWKLRTNLKLPWRPLIAIDGSTSYELNEDF-------KIVRHVESWNVSALEAVLQI 217
LR W ++ P L A+ G ++ +F ++ H ESW++S+ + Q
Sbjct: 150 LRIKWTVKGK---PKSILAAVSGDLIVKVKSEFTLNQISGQVFEHEESWDLSSSSPIAQA 206
Query: 218 F 218
+
Sbjct: 207 Y 207
>At1g16320 unknown protein
Length = 273
Score = 38.9 bits (89), Expect = 0.002
Identities = 36/153 (23%), Positives = 62/153 (39%), Gaps = 13/153 (8%)
Query: 59 LLRLFSPP---QQTSVLSDDIEKQNNDSTVSSVEDVLIIIKS-DYDNDYFVTGNFTSSIY 114
LL + SPP Q +K NN ++ + +++ D T + IY
Sbjct: 51 LLSIQSPPLKDTQVQTRHSSQDKHNNHDRDEFYINLGVAVRTLREDLPLLFTRDLNYDIY 110
Query: 115 TENCIFEDPTIKFSGRDLY-----ARNLKLLVPFFDCASIKLLKIEKEVESDTNFLRASW 169
++ F DP F+G D Y A + F D + L+I + + N + W
Sbjct: 111 RDDITFVDPMNTFTGMDNYKIIFWALRFHGKILFRDIS----LEIFRVWQPSENMILIRW 166
Query: 170 KLRTNLKLPWRPLIAIDGSTSYELNEDFKIVRH 202
L+ ++PW G++ Y+L+ + KI H
Sbjct: 167 NLKGVPRVPWEAKGEFQGTSRYKLDRNGKIYEH 199
>At2g46215 unknown protein
Length = 241
Score = 37.7 bits (86), Expect = 0.005
Identities = 29/133 (21%), Positives = 59/133 (43%), Gaps = 9/133 (6%)
Query: 74 DDIEKQNNDSTVSSVEDVLIIIKSDYDNDYFVTGNFTSSIYTENCIFEDPTIKFSGRDLY 133
++ E+++ S ++ + I+ ++ ++ NF IY ++ +F+DP F G D Y
Sbjct: 74 EEEEEEDKQSYYVNMGHAVRSIREEFPLLFYKELNF--DIYRDDIVFKDPMNTFMGIDNY 131
Query: 134 ARNLKLLV----PFFDCASIKLLKIEKEVESDTNFLRASWKLRTNLKLPWRPLIAIDGST 189
L FF + ++ + + E N L W + + PW DG++
Sbjct: 132 KSIFGALRFHGRIFFRALCVDIVSVWQPTE---NTLMIRWTVHGIPRGPWETRGRFDGTS 188
Query: 190 SYELNEDFKIVRH 202
Y+ +++ KI H
Sbjct: 189 EYKFDKNGKIYEH 201
>At2g33210 mitochondrial chaperonin (HSP60)
Length = 585
Score = 37.7 bits (86), Expect = 0.005
Identities = 39/144 (27%), Positives = 63/144 (43%), Gaps = 11/144 (7%)
Query: 38 MVREKNETPQVLKIAVSGVTELLRLFSPPQQTSVLSDDIEKQNNDSTVSSVEDVL-IIIK 96
M+ E QV I+ +G E+ L + +T I Q+ + + +E V + I
Sbjct: 168 MISTSEEIAQVGTISANGDREIGELIAKAMETVGKEGVITIQDGKTLFNELEVVEGMKID 227
Query: 97 SDYDNDYFVTGNFTSSIYTENCIFEDPTIKFSGRDLYARNLKLLVPFFDCASIK---LLK 153
Y + YF+T T+ C EDP I + + N+ +V + A K LL
Sbjct: 228 RGYISPYFITNP-----KTQKCELEDPLILIHEKKI--SNINAMVKVLELALKKQRPLLI 280
Query: 154 IEKEVESDTNFLRASWKLRTNLKL 177
+ ++VESD KLR N+K+
Sbjct: 281 VAEDVESDALATLILNKLRANIKV 304
>At1g79510 unknown protein
Length = 275
Score = 34.7 bits (78), Expect = 0.042
Identities = 26/102 (25%), Positives = 43/102 (41%), Gaps = 9/102 (8%)
Query: 106 TGNFTSSIYTENCIFEDPTIKFSGRDLY-----ARNLKLLVPFFDCASIKLLKIEKEVES 160
T + IY ++ DP FSG D Y A + F D + L+I + +
Sbjct: 104 TKDLNYDIYRDDITLVDPMNTFSGIDNYKLIFWALRFHGKILFRDIS----LEIFRVWQP 159
Query: 161 DTNFLRASWKLRTNLKLPWRPLIAIDGSTSYELNEDFKIVRH 202
N + W L+ ++PW G++ Y+L+ + KI H
Sbjct: 160 SENMILIRWNLKGVPRVPWEAKGEFQGTSRYKLDRNGKIYEH 201
>At5g20140 unknown protein
Length = 378
Score = 31.6 bits (70), Expect = 0.35
Identities = 41/188 (21%), Positives = 77/188 (40%), Gaps = 24/188 (12%)
Query: 42 KNETPQVLKIAVSGVTELLRLFSPPQQTSVLSDDIEKQ--NNDSTVSSVEDVLIIIKSDY 99
++ T + L + VT LR +LS ++ K+ + STV+ +E+++ + D
Sbjct: 22 RHVTTRFLPVPRRNVTTRLR--------PILSLEVGKEVASAPSTVN-MEELVGFLYEDL 72
Query: 100 DNDYFVTGNFTSSIYTENCIFEDPTIKFSGRDLYARNLKLLVPFFDCASIKLLKIEKEVE 159
+ F + Y E F DP K Y N+ L F ++ +
Sbjct: 73 PH-LFDDQGIDKTAYDERVKFRDPITKHDTISGYLFNIAFLKNIFT----PQFQLHWAKQ 127
Query: 160 SDTNFLRASWKLRTN-LKLPWRPLIAIDGSTSYELN-EDFKIVRHVESW------NVSAL 211
+ + W + + LPW+P + G + E+N E K H++ W + +L
Sbjct: 128 TGPYEITTRWTMVMKFIPLPWKPELVFTGLSIMEVNPETNKFCSHLDLWDSIKNNDYFSL 187
Query: 212 EAVLQIFK 219
E ++ +FK
Sbjct: 188 EGLVDVFK 195
>At2g16270 unknown protein
Length = 759
Score = 30.0 bits (66), Expect = 1.0
Identities = 27/121 (22%), Positives = 53/121 (43%), Gaps = 9/121 (7%)
Query: 8 KTRTPPLQLRKQHCSNSYTPSSRNTCAIYNMVREKNETPQVLKIAVSGVTELLRLFSPPQ 67
+ T P+QL + N +P+ + V + N +P+ K +S E+ R FS +
Sbjct: 64 ENETKPVQLTPKGSKNFMSPT-------ISAVSKINASPR--KRVLSDKNEMSRSFSDVK 114
Query: 68 QTSVLSDDIEKQNNDSTVSSVEDVLIIIKSDYDNDYFVTGNFTSSIYTENCIFEDPTIKF 127
+ D+ + + S DVL I D + + + + T + + E ++E+ I +
Sbjct: 115 GLILEDDNKRNHHRAKSCVSFSDVLHTICIDDEKKFVESHDMTVTDFDEKEVYENKGITY 174
Query: 128 S 128
S
Sbjct: 175 S 175
>At2g21070 unknown protein
Length = 374
Score = 29.6 bits (65), Expect = 1.3
Identities = 17/58 (29%), Positives = 28/58 (47%), Gaps = 3/58 (5%)
Query: 65 PPQQTSVLSDDIEKQNNDSTVSSVEDVLIIIKSDY-DNDYFVTGNFTSSIYTENCIFE 121
PPQ +SV ++E + T+ ++ +KSDY DN F+ + EN F+
Sbjct: 180 PPQCSSV--PEVENTEREKTIQEEAEISATVKSDYHDNKSFIEPAVLLGVVKENETFD 235
>At2g17630 putative phosphoserine aminotransferase
Length = 422
Score = 29.3 bits (64), Expect = 1.8
Identities = 27/100 (27%), Positives = 44/100 (44%), Gaps = 11/100 (11%)
Query: 47 QVLKIAVSGVTELLRLFSPPQQTSVLSDDIEKQNNDSTVSSVEDVLIIIKSDYDNDYFVT 106
+ L I ++L +L P + SVL +T L + KSD DY VT
Sbjct: 104 EFLSIIQKAESDLRQLLEIPSEYSVLF-----LQGGATTQFAALPLNLCKSDDSVDYIVT 158
Query: 107 GNFTSSIYTENCIFEDPTIKFSGR-DLYARNLKLLVPFFD 145
G++ + E + +P + +SG+ + Y + VP FD
Sbjct: 159 GSWGDKAFKEAKKYCNPKVIWSGKSEKYTK-----VPTFD 193
>At1g78560 Unknown protein (T30F21.11)
Length = 401
Score = 28.9 bits (63), Expect = 2.3
Identities = 16/29 (55%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
Query: 3 TESLIKTRTPPLQLRKQHCSN-SYTPSSR 30
+ SL K+R PL LR CS SY+PSSR
Sbjct: 18 SNSLHKSRLTPLHLRTISCSRLSYSPSSR 46
>At3g48060 putative protein
Length = 1611
Score = 28.5 bits (62), Expect = 3.0
Identities = 18/53 (33%), Positives = 28/53 (51%), Gaps = 3/53 (5%)
Query: 64 SPPQQTSVLSDDIEKQNNDSTVSSVEDVLIIIKSDYDNDYFVTGNFTSSIYTE 116
SPP T V+ ++I +DS V+D+ +KS+ D +T SS+ TE
Sbjct: 894 SPPLPTKVIKENIL---DDSDSGEVKDIKTDVKSEADCTSDLTKRVASSMLTE 943
>At3g56410 putative protein
Length = 1535
Score = 28.1 bits (61), Expect = 3.9
Identities = 26/112 (23%), Positives = 45/112 (39%), Gaps = 2/112 (1%)
Query: 3 TESLIKTRTPPLQLRKQHCSNSYTPSSRNTCAIYNMVREKNETPQVLKIAVSGVTELLRL 62
+E++ +T L+ ++ S T N C I EKNE + +I G E L +
Sbjct: 539 SETIGETSKIHLEQSQEAHSEKSTEMDNNICDIEEPEYEKNEIIKPEEIVGEGSGESLEV 598
Query: 63 FSPPQQTSVLSDDIEKQNNDSTVSSVEDVLIIIKSDYDNDYFVTGNFTSSIY 114
P Q +S+ E+ + S ++ K +Y ++ V T Y
Sbjct: 599 --PVNQNERMSESSEEDERVAERSVSHSEELLYKKEYVSETRVQSENTGDTY 648
>At1g67490 alpha-glucosidase I
Length = 852
Score = 28.1 bits (61), Expect = 3.9
Identities = 14/43 (32%), Positives = 24/43 (55%), Gaps = 3/43 (6%)
Query: 74 DDIEKQNNDSTVSSVEDVLIIIKSDYDND---YFVTGNFTSSI 113
D + K+N +STV + + ++ + YD+D YF GN T +
Sbjct: 625 DKLSKENYNSTVKLLSNFNLLNQMHYDSDYGAYFDFGNHTEKV 667
>At4g31880 unknown protein
Length = 873
Score = 27.7 bits (60), Expect = 5.1
Identities = 24/90 (26%), Positives = 37/90 (40%), Gaps = 3/90 (3%)
Query: 4 ESLIKTRTPPLQLRKQHCSNSYTPSSRNTCAIYNMVREKNETPQVLKIAVSGVTELLRLF 63
+S IK T P +L + P + A + EKN++ Q+L SG E +
Sbjct: 377 DSDIKEETEPAELLDSKDVLTSPPVDSSVTAATSSENEKNKSVQILPSKTSG-DETANVS 435
Query: 64 SPPQQTSVLSDDIEKQ--NNDSTVSSVEDV 91
SP + + K+ N SS E+V
Sbjct: 436 SPSMAEELPEQSVPKKTANQKKKESSTEEV 465
>At3g49360 6-phosphogluconolactonase - like protein
Length = 259
Score = 27.3 bits (59), Expect = 6.7
Identities = 28/122 (22%), Positives = 52/122 (41%), Gaps = 11/122 (9%)
Query: 106 TGNFTSSIYTENCIFEDPTIKFSGRDLYARNLKLL-VPFFDC---ASIKLLKIEKEV--- 158
T + +S E +F T+ SG DL A KLL P+ D + + +++ V
Sbjct: 23 TADLSSKFCKERGVF---TVVLSGGDLIAWLWKLLEAPYIDSIEWSKWHIFWVDERVCAW 79
Query: 159 -ESDTNFLRASWKLRTNLKLPWRPLIAIDGSTSYELNEDFKIVRHVESWNVSALEAVLQI 217
+D+N+ A + + +P + AID E N + R+ E + +++
Sbjct: 80 DHADSNYKLAYDGFLSKVPVPAENIYAIDNGLGAEGNAELAAERYEECLKQKVNQNIIRT 139
Query: 218 FK 219
+K
Sbjct: 140 YK 141
>At1g15660 unknown protein
Length = 710
Score = 27.3 bits (59), Expect = 6.7
Identities = 15/31 (48%), Positives = 19/31 (60%), Gaps = 4/31 (12%)
Query: 110 TSSIYTENCIFEDPTIKFSGRDLYARNLKLL 140
+SS+YTE EDP +SG L+ R LK L
Sbjct: 7 SSSLYTE----EDPLQAYSGLSLFPRTLKSL 33
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.134 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,149,488
Number of Sequences: 26719
Number of extensions: 215871
Number of successful extensions: 574
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 565
Number of HSP's gapped (non-prelim): 19
length of query: 225
length of database: 11,318,596
effective HSP length: 96
effective length of query: 129
effective length of database: 8,753,572
effective search space: 1129210788
effective search space used: 1129210788
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC135565.11