BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135504.9 + phase: 0 /pseudo
(1303 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g60650 putative RNA-binding protein 43 0.001
At3g60170 putative protein 41 0.004
At1g32590 hypothetical protein, 5' partial 41 0.005
At2g07550 putative retroelement pol polyprotein 39 0.026
At4g10460 putative retrotransposon 38 0.033
At3g45520 copia-like polyprotein 36 0.17
At3g43590 putative protein 36 0.17
At2g05390 putative retroelement pol polyprotein 36 0.17
At1g36110 hypothetical protein 36 0.17
At4g04310 putative transposon protein 35 0.22
At2g13890 hypothetical protein 35 0.22
At2g11220 putative retroelement pol polyprotein 35 0.37
At4g36020 glycine-rich protein 34 0.63
At4g31580 splicing factor 9G8-like SR protein / SRZ-22 34 0.63
At4g12200 putative protein 33 0.82
At4g05360 putative protein 33 1.1
At3g26420 RNA-binding protein, putative 33 1.1
At5g47430 DNA-binding protein-like 33 1.4
At4g04420 putative transposon protein 33 1.4
At3g02820 CCHC-type zinc finger - like protein 33 1.4
>At1g60650 putative RNA-binding protein
Length = 249
Score = 42.7 bits (99), Expect = 0.001
Identities = 21/65 (32%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
Query: 170 LGKGKIEKKPLESKFGGRFRYGKVLGRGGYKNSRKEDQKGCFNCKKPGHFIADCPDLQKE 229
LG I E K GG ++ GGY + K + CF C++PGH+ DCP +
Sbjct: 35 LGNKVISVNKAEPKVGGE-DVDQLKKGGGYSSRGKGTEDECFKCRRPGHWARDCPSTGDD 93
Query: 230 KSKSR 234
+ + R
Sbjct: 94 RERFR 98
>At3g60170 putative protein
Length = 1339
Score = 41.2 bits (95), Expect = 0.004
Identities = 20/76 (26%), Positives = 39/76 (51%)
Query: 176 EKKPLESKFGGRFRYGKVLGRGGYKNSRKEDQKGCFNCKKPGHFIADCPDLQKEKSKSRP 235
E++P + + G FR + GRG ++ C+ C GHF +CP+ +K + +
Sbjct: 218 EERPSQGRGRGVFRGSRGRGRGRGRSGTNRAIVECYKCHNLGHFQYECPEWEKNANYAEL 277
Query: 236 KKQSELVKTAGVPKGK 251
+++ EL+ A V + +
Sbjct: 278 EEEEELLLMAYVEQNQ 293
>At1g32590 hypothetical protein, 5' partial
Length = 1263
Score = 40.8 bits (94), Expect = 0.005
Identities = 25/72 (34%), Positives = 38/72 (52%), Gaps = 12/72 (16%)
Query: 170 LGKGKIEKKPLESKF-----GGRFRYG---KVLGRGGYKNSRK----EDQKGCFNCKKPG 217
L + +E++ L+++ GGR R G + GRGGY+ + D CF C K G
Sbjct: 160 LSRHDVEERVLKAETQWRPDGGRGRGGSPSRGRGRGGYQGRGRGYVNRDTVECFKCHKMG 219
Query: 218 HFIADCPDLQKE 229
H+ A+CP +KE
Sbjct: 220 HYKAECPSWEKE 231
>At2g07550 putative retroelement pol polyprotein
Length = 1356
Score = 38.5 bits (88), Expect = 0.026
Identities = 18/53 (33%), Positives = 30/53 (55%), Gaps = 5/53 (9%)
Query: 191 GKVLGRGGYKNSRKEDQKGCFNCKKPGHFIADCPDLQKEKSKSRPKKQSELVK 243
G+ G+G K + + + GC+ C + GHF + CP+ K + KQS++VK
Sbjct: 229 GEQKGKGKGKKGKSKKKPGCWTCGEEGHFRSSCPNQNKPQF-----KQSQVVK 276
>At4g10460 putative retrotransposon
Length = 1230
Score = 38.1 bits (87), Expect = 0.033
Identities = 16/43 (37%), Positives = 24/43 (55%)
Query: 192 KVLGRGGYKNSRKEDQKGCFNCKKPGHFIADCPDLQKEKSKSR 234
K G G SR + KGC+ C + GHF CP+ K+++K +
Sbjct: 230 KEKGNKGRSRSRSKGWKGCWICGEEGHFKTSCPNKGKQQNKGK 272
>At3g45520 copia-like polyprotein
Length = 1363
Score = 35.8 bits (81), Expect = 0.17
Identities = 19/69 (27%), Positives = 36/69 (51%), Gaps = 1/69 (1%)
Query: 172 KGKIEKKPLESKFGGRFRYGKVLGRGGYKNSRKEDQKGCFNCKKPGHFIADCPDLQKEKS 231
KG+ E ++ K R R + +G K S+ + ++GC+ C + GH + CP+ K +
Sbjct: 211 KGQAEGLYVKDKAENRGR-SEQKDKGKGKRSKSKSKRGCWICGEDGHLKSTCPNKNKPQF 269
Query: 232 KSRPKKQSE 240
K++ + E
Sbjct: 270 KNQGSNKGE 278
>At3g43590 putative protein
Length = 551
Score = 35.8 bits (81), Expect = 0.17
Identities = 17/38 (44%), Positives = 21/38 (54%), Gaps = 2/38 (5%)
Query: 197 GGYKNSRKEDQKG--CFNCKKPGHFIADCPDLQKEKSK 232
G ++ K+ KG C+ CKK GH DCPD K SK
Sbjct: 195 GSLEHGAKQCSKGHDCYICKKTGHRAKDCPDKYKNGSK 232
Score = 32.3 bits (72), Expect = 1.8
Identities = 18/64 (28%), Positives = 27/64 (42%), Gaps = 12/64 (18%)
Query: 204 KEDQKGCFNCKKPGHFIADCPDLQK------------EKSKSRPKKQSELVKTAGVPKGK 251
+E Q C+ C GHF +CP+ + KS+ + K+ SE T GK
Sbjct: 352 RESQTLCYRCNGSGHFARECPNSSQVSKRDRETSTTSHKSRKKNKENSEHDSTPHESNGK 411
Query: 252 RETK 255
+ K
Sbjct: 412 TKKK 415
>At2g05390 putative retroelement pol polyprotein
Length = 1307
Score = 35.8 bits (81), Expect = 0.17
Identities = 23/75 (30%), Positives = 39/75 (51%), Gaps = 7/75 (9%)
Query: 172 KGKIEKKPLESKF---GGRFR---YGKVLGRGGYKNSRKEDQKG-CFNCKKPGHFIADCP 224
+GK+ ES + GGR R GRGGY +++ K C+ C K GH+ ++C
Sbjct: 170 QGKLMYANSESSYDTRGGRGRGRGRSSGRGRGGYGYQQRDKSKVICYRCDKTGHYASECL 229
Query: 225 DLQKEKSKSRPKKQS 239
D + K++ ++Q+
Sbjct: 230 DRLLKLIKAQEQQQN 244
>At1g36110 hypothetical protein
Length = 745
Score = 35.8 bits (81), Expect = 0.17
Identities = 14/34 (41%), Positives = 20/34 (58%)
Query: 195 GRGGYKNSRKEDQKGCFNCKKPGHFIADCPDLQK 228
GRG + SR + C+ C K GH+ ++CPD K
Sbjct: 233 GRGRGRGSRDTSKVTCYRCDKLGHYASNCPDSVK 266
>At4g04310 putative transposon protein
Length = 1011
Score = 35.4 bits (80), Expect = 0.22
Identities = 18/51 (35%), Positives = 25/51 (48%), Gaps = 8/51 (15%)
Query: 200 KNSRKEDQKG--CFNCKKPGHFIADCPD------LQKEKSKSRPKKQSELV 242
K+S +G C+NC+K GH +A CP+ E S P SE+V
Sbjct: 388 KSSNLSKSRGVTCYNCRKKGHLVATCPEKFELTNTSLESKLSSPNSSSEVV 438
>At2g13890 hypothetical protein
Length = 524
Score = 35.4 bits (80), Expect = 0.22
Identities = 18/51 (35%), Positives = 25/51 (48%), Gaps = 8/51 (15%)
Query: 200 KNSRKEDQKG--CFNCKKPGHFIADCPD------LQKEKSKSRPKKQSELV 242
K+S +G C+NC+K GH +A CP+ E S P SE+V
Sbjct: 27 KSSNLSKSRGVTCYNCRKKGHLVATCPEKLELTNTSLESKLSSPNSSSEVV 77
>At2g11220 putative retroelement pol polyprotein
Length = 358
Score = 34.7 bits (78), Expect = 0.37
Identities = 17/57 (29%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
Query: 186 GRFRYGKVLGRGGYKNSRKEDQKGCFNCKKPGHFIADCPD--LQKEKSKSRPKKQSE 240
G Y K GRG + R + + C K GH+ +DCPD L+ ++++ K ++
Sbjct: 95 GGCSYYKGRGRGRFNGGRDTSKIMYYRCDKLGHYASDCPDRLLKLQETQENEKDDTQ 151
>At4g36020 glycine-rich protein
Length = 299
Score = 33.9 bits (76), Expect = 0.63
Identities = 16/52 (30%), Positives = 21/52 (39%)
Query: 195 GRGGYKNSRKEDQKGCFNCKKPGHFIADCPDLQKEKSKSRPKKQSELVKTAG 246
G GG R +GC+NC GHF DC + K ++ T G
Sbjct: 119 GGGGGGERRSRGGEGCYNCGDTGHFARDCTSAGNGDQRGATKGGNDGCYTCG 170
Score = 31.6 bits (70), Expect = 3.1
Identities = 12/32 (37%), Positives = 16/32 (49%)
Query: 192 KVLGRGGYKNSRKEDQKGCFNCKKPGHFIADC 223
K +G G + + K GC+ C GHF DC
Sbjct: 182 KSVGNGDQRGAVKGGNDGCYTCGDVGHFARDC 213
>At4g31580 splicing factor 9G8-like SR protein / SRZ-22
Length = 200
Score = 33.9 bits (76), Expect = 0.63
Identities = 19/66 (28%), Positives = 31/66 (46%), Gaps = 3/66 (4%)
Query: 174 KIEKKPLESKFGGRFRYGKVLGRGGYKNSRKEDQKGCFNCKKPGHFIADCPD---LQKEK 230
++E+ + GG R G G GG + R C+ C + GHF +C + + +
Sbjct: 65 RVEQSHNRGERGGGGRGGDRGGGGGGRGGRGGSDLKCYECGETGHFARECRNRGGTGRRR 124
Query: 231 SKSRPK 236
SKSR +
Sbjct: 125 SKSRSR 130
>At4g12200 putative protein
Length = 200
Score = 33.5 bits (75), Expect = 0.82
Identities = 17/49 (34%), Positives = 27/49 (54%), Gaps = 5/49 (10%)
Query: 202 SRKEDQKG-CFNCKKPGHFIADCPDLQKEKSKSRPKKQSELVKTAGVPK 249
S+ + +KG C+ C + GH+ DCP EK PK+ + + A VP+
Sbjct: 152 SKSKGEKGACWICGEDGHYKNDCPSRNSEK----PKEHAYKAQEASVPR 196
>At4g05360 putative protein
Length = 735
Score = 33.1 bits (74), Expect = 1.1
Identities = 17/43 (39%), Positives = 23/43 (52%), Gaps = 3/43 (6%)
Query: 199 YKNSRKEDQKGCFNCKKPGHFIADCPDLQKEKSKSRPKKQSEL 241
YK+S+ K CF CK H ++C +L KEK K SE+
Sbjct: 372 YKSSKG---KRCFECKGFRHMCSECANLMKEKEKKFIMSDSEI 411
>At3g26420 RNA-binding protein, putative
Length = 245
Score = 33.1 bits (74), Expect = 1.1
Identities = 14/32 (43%), Positives = 15/32 (46%), Gaps = 5/32 (15%)
Query: 198 GYKNSRKEDQKG-----CFNCKKPGHFIADCP 224
GY R G CF C KPGHF +CP
Sbjct: 103 GYDRDRSRPSGGRGGGDCFKCGKPGHFARECP 134
>At5g47430 DNA-binding protein-like
Length = 889
Score = 32.7 bits (73), Expect = 1.4
Identities = 24/67 (35%), Positives = 30/67 (43%), Gaps = 10/67 (14%)
Query: 186 GRFRYGKVLGRGGYKNSRKEDQKG--CFNCKKPGHFIADCPDLQKEKSKSRPKKQSELVK 243
GR G++ GRG + RK G C C PGHFI CP + P + VK
Sbjct: 181 GRGMPGRMNGRG-FGMERKTPPPGYVCHRCNIPGHFIQHCP------TNGDPNYDVKRVK 233
Query: 244 -TAGVPK 249
G+PK
Sbjct: 234 PPTGIPK 240
>At4g04420 putative transposon protein
Length = 1008
Score = 32.7 bits (73), Expect = 1.4
Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Query: 190 YGKVLGRGGYKN-SRKEDQKGCFNCKKPGHFIADCPDLQKEKSK 232
+G+ G Y++ S K D+ C C+ GH A+CP L+++ K
Sbjct: 261 FGRNQGTDRYRDRSSKRDEIQCHECQGYGHIKAECPSLKRKDLK 304
>At3g02820 CCHC-type zinc finger - like protein
Length = 282
Score = 32.7 bits (73), Expect = 1.4
Identities = 18/70 (25%), Positives = 26/70 (36%), Gaps = 3/70 (4%)
Query: 209 GCFNCKKPGHFIADCPD---LQKEKSKSRPKKQSELVKTAGVPKGKRETKVMVPRQRVFK 265
GCF C +PGH+ DCP + S S S++ + + P +V K
Sbjct: 7 GCFKCGRPGHWSRDCPSSAPVAGNNSVSSSSAPSQIPNNEFQRSSSKSGTSIAPAPKVTK 66
Query: 266 AHDWRESFVP 275
R P
Sbjct: 67 TRVQRPKLTP 76
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.382 0.170 0.717
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,338,174
Number of Sequences: 26719
Number of extensions: 728210
Number of successful extensions: 4980
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 4916
Number of HSP's gapped (non-prelim): 74
length of query: 1303
length of database: 11,318,596
effective HSP length: 111
effective length of query: 1192
effective length of database: 8,352,787
effective search space: 9956522104
effective search space used: 9956522104
T: 11
A: 40
X1: 13 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 35 (21.8 bits)
S2: 66 (30.0 bits)
Medicago: description of AC135504.9