BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135504.16 - phase: 0 /pseudo
(343 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g29480 hypothetical protein 69 4e-12
At4g03650 putative reverse transcriptase 68 6e-12
At3g31970 hypothetical protein 67 1e-11
At3g43830 putative protein 66 2e-11
At2g04670 putative retroelement pol polyprotein 66 3e-11
At2g14650 putative retroelement pol polyprotein 62 6e-10
At4g03840 putative transposon protein 61 8e-10
At2g10780 pseudogene 60 1e-09
At2g06470 putative retroelement pol polyprotein 60 1e-09
At2g07700 putative retroelement pol polyprotein 59 5e-09
At3g43090 putative protein 54 9e-08
At3g42290 putative protein 54 2e-07
At4g10580 putative reverse-transcriptase -like protein 49 4e-06
At4g08100 putative polyprotein 42 6e-04
At1g36120 putative reverse transcriptase gb|AAD22339.1 37 0.012
At1g36550 hypothetical protein 36 0.035
At1g67020 hypothetical protein 35 0.078
At2g14640 putative retroelement pol polyprotein 31 1.1
At3g31360 hypothetical protein 30 1.9
At5g11530 putative protein 29 3.3
>At3g29480 hypothetical protein
Length = 718
Score = 68.9 bits (167), Expect = 4e-12
Identities = 41/121 (33%), Positives = 57/121 (46%), Gaps = 2/121 (1%)
Query: 86 VRMLETFLRNHPPTFKGRYDPDGTQKWLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDW 145
+RM+E R F G P+ W VER F +C +V H L +A W
Sbjct: 37 LRMMEQLQRIGIGYFSGGTSPEEADSWRSRVERNFGSSRCPVEYRVDLAVHFLEGDAHLW 96
Query: 146 WVSLLPVLEQDGAVVTWAVFRREFLNRYFPEDVRGKNEIEFLELKHGDMSVTKYAAKFVE 205
W S+ Q A ++WA F EF +YFP++ + E FLEL G+ SV +Y +F
Sbjct: 97 WKSVTTRRRQ--ANMSWADFVAEFNAKYFPQEALDRMEAHFLELTQGERSVREYDREFNR 154
Query: 206 L 206
L
Sbjct: 155 L 155
>At4g03650 putative reverse transcriptase
Length = 839
Score = 68.2 bits (165), Expect = 6e-12
Identities = 41/121 (33%), Positives = 56/121 (45%), Gaps = 2/121 (1%)
Query: 86 VRMLETFLRNHPPTFKGRYDPDGTQKWLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDW 145
+RM++ R F G P+ W +VER F +C +V H L +A W
Sbjct: 142 LRMMKQLQRIGTEYFSGGTSPEEADSWRSQVERNFGSSRCPAEYRVDLTVHFLEGDAHLW 201
Query: 146 WVSLLPVLEQDGAVVTWAVFRREFLNRYFPEDVRGKNEIEFLELKHGDMSVTKYAAKFVE 205
W S+ Q A ++WA F EF +YFP + + E FLEL G SV +Y KF
Sbjct: 202 WRSVTARRRQ--ADMSWADFMAEFNAKYFPREALDRMEARFLELTQGVRSVREYDRKFNR 259
Query: 206 L 206
L
Sbjct: 260 L 260
>At3g31970 hypothetical protein
Length = 1329
Score = 67.4 bits (163), Expect = 1e-11
Identities = 47/151 (31%), Positives = 65/151 (42%), Gaps = 6/151 (3%)
Query: 60 PAIAAALEAVA*AVGQQPNAAAGNDGV----RMLETFLRNHPPTFKGRYDPDGTQKWLKE 115
P + VA V + AA + V RM+E R F G P+ W
Sbjct: 114 PGVVPVQAPVAPRVAEVQQRAAVAEEVPSYLRMMEQLQRIGTRYFFGGTSPEEADSWKSR 173
Query: 116 VERIFRVMQCSEVQKVRFGTHMLAEEADDWWVSLLPVLEQDGAVVTWAVFRREFLNRYFP 175
VE F +C +V H L +A WW S+ Q A ++WA F EF +YFP
Sbjct: 174 VEHNFGSSRCPAEYRVDLAVHFLEGDAHLWWRSVTARRRQ--AHMSWADFVAEFNAKYFP 231
Query: 176 EDVRGKNEIEFLELKHGDMSVTKYAAKFVEL 206
++ + E FLEL G+ SV +Y +F L
Sbjct: 232 QEALDRMEARFLELTQGERSVREYEREFNRL 262
>At3g43830 putative protein
Length = 329
Score = 66.2 bits (160), Expect = 2e-11
Identities = 38/111 (34%), Positives = 53/111 (47%), Gaps = 1/111 (0%)
Query: 99 TFKGRYDPDGTQKWLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDWWVSLLPVLEQDGA 158
TF G DP W +E F+ M+C + L EA +WW + EQ G
Sbjct: 115 TFNGGTDPVKAYNWRNMLECKFKSMRCPVEFWRDLASCYLRGEAQEWW-ERVKQREQVGC 173
Query: 159 VVTWAVFRREFLNRYFPEDVRGKNEIEFLELKHGDMSVTKYAAKFVELAKF 209
V W+ F+ EF RY PE+ E++FL L+ G +V KY +F L +F
Sbjct: 174 VDQWSFFKEEFTRRYLPEETIDDLEMKFLRLQQGTKTVRKYEKEFHSLERF 224
>At2g04670 putative retroelement pol polyprotein
Length = 1411
Score = 65.9 bits (159), Expect = 3e-11
Identities = 39/124 (31%), Positives = 57/124 (45%), Gaps = 2/124 (1%)
Query: 86 VRMLETFLRNHPPTFKGRYDPDGTQKWLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDW 145
+RM+E R F P+ W V+R F +C +V H L +A W
Sbjct: 124 LRMMEQLQRIGTWYFSSGTSPEEADSWRSRVQRNFGSSRCPAEYRVDLAVHFLEGDAHLW 183
Query: 146 WVSLLPVLEQDGAVVTWAVFRREFLNRYFPEDVRGKNEIEFLELKHGDMSVTKYAAKFVE 205
W S+ Q A ++WA F EF +YFP++ + E FLEL G+ SV +Y +F
Sbjct: 184 WRSVTARRRQ--ADMSWADFVAEFNAKYFPQEALDRMEARFLELTQGEWSVREYDREFNR 241
Query: 206 LAKF 209
L +
Sbjct: 242 LLAY 245
>At2g14650 putative retroelement pol polyprotein
Length = 1328
Score = 61.6 bits (148), Expect = 6e-10
Identities = 35/107 (32%), Positives = 49/107 (45%), Gaps = 2/107 (1%)
Query: 86 VRMLETFLRNHPPTFKGRYDPDGTQKWLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDW 145
+RM+E R F G P+ W VER F +C ++ H L +A W
Sbjct: 142 LRMMEQLQRIGTGYFSGGTSPEVADSWRSRVERNFGSSRCPAEYRIDLAVHFLEGDAHLW 201
Query: 146 WVSLLPVLEQDGAVVTWAVFRREFLNRYFPEDVRGKNEIEFLELKHG 192
W S+ Q A ++WA F EF +YFP++ + E FLEL G
Sbjct: 202 WRSVTARRRQ--ADISWADFVAEFNAKYFPQEALDRMEAHFLELTQG 246
>At4g03840 putative transposon protein
Length = 973
Score = 61.2 bits (147), Expect = 8e-10
Identities = 38/138 (27%), Positives = 65/138 (46%), Gaps = 3/138 (2%)
Query: 73 VGQQPN-AAAGNDGVRMLETFLRNHPPTFKGRYDPDGTQKWLKEVERIFRVMQCSEVQKV 131
V QPN D + +LE R F G DP +W ++R F+ +C E +
Sbjct: 119 VNPQPNHGRKRGDYLSLLEHVSRLGTRHFMGSTDPIVADEWRSRLKRNFKSTRCPEDYQR 178
Query: 132 RFGTHMLAEEADDWWVSLLPVLEQDGAVVTWAVFRREFLNRYFPEDVRGKNEIEFLELKH 191
H L +A +WW+++ + V ++A F EF +YFP + + E +L+L
Sbjct: 179 DIAVHFLERDAHNWWLTV--EKRRGDEVRSFADFEDEFNKKYFPPEAWDRLECAYLDLVQ 236
Query: 192 GDMSVTKYAAKFVELAKF 209
G+ +V +Y +F L ++
Sbjct: 237 GNRTVREYDEEFNRLRRY 254
>At2g10780 pseudogene
Length = 1611
Score = 60.5 bits (145), Expect = 1e-09
Identities = 38/138 (27%), Positives = 65/138 (46%), Gaps = 3/138 (2%)
Query: 73 VGQQPN-AAAGNDGVRMLETFLRNHPPTFKGRYDPDGTQKWLKEVERIFRVMQCSEVQKV 131
V QPN D + +LE R F G DP +W ++R F+ +C E +
Sbjct: 154 VNPQPNHGRKRGDYLSLLEHVSRLGTRHFMGSTDPIVADEWRSRLKRNFKSTRCPEDYQR 213
Query: 132 RFGTHMLAEEADDWWVSLLPVLEQDGAVVTWAVFRREFLNRYFPEDVRGKNEIEFLELKH 191
H L +A +WW+++ + V ++A F EF +YFP + + E +L+L
Sbjct: 214 DIAVHFLEGDAHNWWLTV--EKRRGDEVRSFADFEDEFNKKYFPPEAWDRLECAYLDLVQ 271
Query: 192 GDMSVTKYAAKFVELAKF 209
G+ +V +Y +F L ++
Sbjct: 272 GNRTVREYDEEFNRLRRY 289
>At2g06470 putative retroelement pol polyprotein
Length = 899
Score = 60.5 bits (145), Expect = 1e-09
Identities = 38/138 (27%), Positives = 65/138 (46%), Gaps = 3/138 (2%)
Query: 73 VGQQPN-AAAGNDGVRMLETFLRNHPPTFKGRYDPDGTQKWLKEVERIFRVMQCSEVQKV 131
V QPN D + +LE R F G DP +W ++R F+ +C E +
Sbjct: 119 VNPQPNHGRKRGDYLSLLEHVSRLGTRHFMGSTDPIVADEWRSRLKRNFKSTRCPEDYQR 178
Query: 132 RFGTHMLAEEADDWWVSLLPVLEQDGAVVTWAVFRREFLNRYFPEDVRGKNEIEFLELKH 191
H L +A +WW+++ + V ++A F EF +YFP + + E +L+L
Sbjct: 179 DIAVHFLEGDAHNWWLTV--EKRRGDEVRSFADFEDEFNKKYFPPEAWDRLECAYLDLVQ 236
Query: 192 GDMSVTKYAAKFVELAKF 209
G+ +V +Y +F L ++
Sbjct: 237 GNRTVREYDEEFNRLRRY 254
>At2g07700 putative retroelement pol polyprotein
Length = 543
Score = 58.5 bits (140), Expect = 5e-09
Identities = 27/89 (30%), Positives = 49/89 (54%), Gaps = 2/89 (2%)
Query: 100 FKGRYDPDGTQKWLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDWWVSLLPVLEQDGAV 159
FKG ++ KWL+++E F + +C + K + + L ++A W S+ G +
Sbjct: 53 FKGEHNTVVADKWLRDLEMNFEISRCPDNFKRQIAVNFLDKDARIWRESV--TARNQGQM 110
Query: 160 VTWAVFRREFLNRYFPEDVRGKNEIEFLE 188
++W FRREF +YFP + R + E +F++
Sbjct: 111 ISWEAFRREFEKKYFPPEARDRLEQQFMK 139
>At3g43090 putative protein
Length = 357
Score = 54.3 bits (129), Expect = 9e-08
Identities = 34/113 (30%), Positives = 49/113 (43%), Gaps = 7/113 (6%)
Query: 100 FKGRYDPDGTQKWLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDWWVSLL---PVLEQD 156
F G DP W K +E F +C + H L EEA WW +L PV
Sbjct: 131 FTGGIDPVKADDWRKLLENNFENARCPVEFQKDIIVHFLREEASHWWDGVLGNTPVQH-- 188
Query: 157 GAVVTWAVFRREFLNRYFPEDVRGKNEIEFLELKHGDMSVTKYAAKFVELAKF 209
V+ W FR EF ++FP++ E +F EL+ V + + L++F
Sbjct: 189 --VIFWEDFREEFNRKFFPQEAMDSLEDDFEELRQDTKKVRENERELSHLSRF 239
>At3g42290 putative protein
Length = 462
Score = 53.5 bits (127), Expect = 2e-07
Identities = 32/92 (34%), Positives = 46/92 (49%), Gaps = 2/92 (2%)
Query: 112 WLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDWWVSLLPVLEQDGAVVTWAVFRREFLN 171
W V+R F +C +V H L E+A WW S+ Q A ++WA F EF
Sbjct: 166 WRSRVKRNFSSSRCLMEYRVDLAVHFLEEDAHLWWKSVTARRRQ--ADMSWADFVVEFNA 223
Query: 172 RYFPEDVRGKNEIEFLELKHGDMSVTKYAAKF 203
+YF +D + E+ FLEL + SV +Y +F
Sbjct: 224 KYFLQDELDRMEVRFLELTLVERSVWEYDREF 255
>At4g10580 putative reverse-transcriptase -like protein
Length = 1240
Score = 48.9 bits (115), Expect = 4e-06
Identities = 28/92 (30%), Positives = 40/92 (43%), Gaps = 2/92 (2%)
Query: 86 VRMLETFLRNHPPTFKGRYDPDGTQKWLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDW 145
+RM+E R F G P+ W V R F +C +V H L +A W
Sbjct: 138 LRMMEQLQRIDTGYFSGGTSPEEADSWRSRVGRNFGSSRCPAEYRVDLAVHFLEGDAHLW 197
Query: 146 WVSLLPVLEQDGAVVTWAVFRREFLNRYFPED 177
W S+ Q ++WA F EF +YFP++
Sbjct: 198 WRSVTARRRQTD--MSWADFVAEFKAKYFPQE 227
>At4g08100 putative polyprotein
Length = 1054
Score = 41.6 bits (96), Expect = 6e-04
Identities = 28/109 (25%), Positives = 48/109 (43%), Gaps = 16/109 (14%)
Query: 98 PTFKGRYDPDGTQKWLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDWWVSLLPVLE--Q 155
P F G +PD +W +++E +F +C + KV+ A WW L+ +
Sbjct: 97 PAFHGTDNPDTYLEWEQKIELVFLCQECLQSNKVKIAATKFYNYALSWWDQLVTSRRRTR 156
Query: 156 DGAVVTW----AVFRREFLNRYFPED--------VRGKNEIE--FLELK 190
D + TW V R+ F+ Y+ + V+G +E FLE++
Sbjct: 157 DYPIKTWNQLKFVMRKRFVPSYYHRELHQRLRNLVQGSKTVEEYFLEME 205
>At1g36120 putative reverse transcriptase gb|AAD22339.1
Length = 1235
Score = 37.4 bits (85), Expect = 0.012
Identities = 17/42 (40%), Positives = 25/42 (59%)
Query: 165 FRREFLNRYFPEDVRGKNEIEFLELKHGDMSVTKYAAKFVEL 206
F EF +YFP++ + E FLEL G++SV +Y +F L
Sbjct: 157 FVAEFNAKYFPQEALDRMEARFLELTKGELSVREYGREFKRL 198
>At1g36550 hypothetical protein
Length = 353
Score = 35.8 bits (81), Expect = 0.035
Identities = 15/48 (31%), Positives = 23/48 (47%)
Query: 98 PTFKGRYDPDGTQKWLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDW 145
P F G+ DPD +W K +E +F + E+ +VR + A W
Sbjct: 93 PPFHGKNDPDAYLEWEKNIELVFNLQHYIEINRVRVAATEFYDYALSW 140
>At1g67020 hypothetical protein
Length = 659
Score = 34.7 bits (78), Expect = 0.078
Identities = 21/81 (25%), Positives = 34/81 (41%), Gaps = 4/81 (4%)
Query: 104 YDPDGTQKWLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDWWVSLLPVLEQDGAVVTWA 163
+D G +W +VER FRV + + K+ L A W++ + LE W
Sbjct: 115 FDGSGVYEWFSKVERFFRVGRYQDSDKLDLVALSLEGVALKWFLREMSTLE----FRDWN 170
Query: 164 VFRREFLNRYFPEDVRGKNEI 184
F + L R+ P + E+
Sbjct: 171 SFEQRLLARFDPVKIHSSPEV 191
>At2g14640 putative retroelement pol polyprotein
Length = 945
Score = 30.8 bits (68), Expect = 1.1
Identities = 18/67 (26%), Positives = 29/67 (42%)
Query: 103 RYDPDGTQKWLKEVERIFRVMQCSEVQKVRFGTHMLAEEADDWWVSLLPVLEQDGAVVTW 162
R+ KWL + ++ F + Q VRF T L A+ WW + L G +
Sbjct: 110 RFHGGDLTKWLSKAKQYFEYHEGPVEQAVRFTTFHLDGVANGWWQATLRRSATIGQTGSL 169
Query: 163 AVFRREF 169
+ ++ EF
Sbjct: 170 SEYQHEF 176
>At3g31360 hypothetical protein
Length = 297
Score = 30.0 bits (66), Expect = 1.9
Identities = 15/50 (30%), Positives = 23/50 (46%), Gaps = 5/50 (10%)
Query: 129 QKVRFGTHMLAEEADDWWVSLLPVLEQDGAVVTWAVFRREFLNRYFPEDV 178
QKV+ H LA + W L + G++ W + F+N +F E V
Sbjct: 77 QKVQTLPHSLARKGTSWLRQL-----ESGSITNWTDTKHTFMNHFFNESV 121
>At5g11530 putative protein
Length = 1096
Score = 29.3 bits (64), Expect = 3.3
Identities = 16/49 (32%), Positives = 26/49 (52%), Gaps = 3/49 (6%)
Query: 168 EFLNRYFPEDVRGKNEIEFLELKHGDMSVTKYAAKFVELAKFYPYYTAE 216
+F R+FP + K EF+EL+ GD S + Y+ K + + +T E
Sbjct: 883 KFTKRHFPPANQSK---EFIELQTGDSSKSAYSTKQIPFDLYSKRFTQE 928
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.355 0.158 0.577
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,743,005
Number of Sequences: 26719
Number of extensions: 248847
Number of successful extensions: 1221
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1191
Number of HSP's gapped (non-prelim): 21
length of query: 343
length of database: 11,318,596
effective HSP length: 100
effective length of query: 243
effective length of database: 8,646,696
effective search space: 2101147128
effective search space used: 2101147128
T: 11
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC135504.16