BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135320.11 + phase: 0
(165 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g22530 unknown protein 137 2e-33
At4g14830 unknown protein 133 4e-32
At2g29500 putative small heat shock protein 32 0.12
At5g59720 heat shock protein 18 30 0.59
At3g46230 heat shock protein 17 30 0.59
At1g78560 Unknown protein (T30F21.11) 30 0.77
At1g53540 17.6 kDa heat shock protein (AA 1-156) 29 1.3
At5g39760 unknown protein 28 1.7
At1g59860 unknown protein 28 1.7
At4g24560 putative protein 28 2.2
At5g12020 heat shock protein 17.6-II 27 3.8
At4g21870 heat shock protein - like 27 3.8
At1g07400 heat shock like protein 27 3.8
At5g61290 unknown protein 27 5.0
At3g13445 transcription initiation factor TFIID-1 (TATA sequence... 27 6.5
At2g17140 hypothetical protein 27 6.5
At2g25370 hypothetical protein 26 8.5
>At3g22530 unknown protein
Length = 198
Score = 137 bits (346), Expect = 2e-33
Identities = 73/126 (57%), Positives = 90/126 (70%), Gaps = 8/126 (6%)
Query: 38 LRRLPHVFSRILQLPLRSDADVSIEEEPTCFRFVAETDSSL---GHVETHTLHIHPGVTK 94
LRRLPH+F+R+L+LPLRS+ADV++EE CFRFVAET G + + + IHPG+TK
Sbjct: 61 LRRLPHIFNRVLELPLRSEADVAVEERHDCFRFVAETVGLCNGDGEMRAYMVEIHPGITK 120
Query: 95 IVVRA--SHSLHFSLDDLHPDIWRFRLPESVVPELATAVFVDGELIVTVPKAFDDENTPE 152
IVVR S SL SLD+L D+WRFRLPES PEL T VDG+LIVTVPK ++E+
Sbjct: 121 IVVRTNGSSSLGLSLDELELDVWRFRLPESTRPELVTVACVDGDLIVTVPKNAEEEDDD- 179
Query: 153 PIGGGG 158
GGGG
Sbjct: 180 --GGGG 183
>At4g14830 unknown protein
Length = 152
Score = 133 bits (335), Expect = 4e-32
Identities = 73/115 (63%), Positives = 85/115 (73%), Gaps = 4/115 (3%)
Query: 37 KLRRLPHVFSRILQLPLRSDADVSIEEEPTCFRFVAETD-SSLGHVETHTLHIHPGVTKI 95
KLRRLPH+FSR+L+LPL+SDADV++EE CFRFVAETD G V + + IHPGVTKI
Sbjct: 29 KLRRLPHIFSRVLELPLKSDADVAVEESHDCFRFVAETDGGGGGGVRAYMVEIHPGVTKI 88
Query: 96 VVR--ASHSLHFSLDDLHPDIWRFRLPESVVPELATA-VFVDGELIVTVPKAFDD 147
+VR S SL SLD+L D+WRFRLPES PEL T DGELIVTVPK D+
Sbjct: 89 LVRTNGSSSLGLSLDELELDVWRFRLPESTRPELVTVDCDGDGELIVTVPKIEDN 143
>At2g29500 putative small heat shock protein
Length = 153
Score = 32.3 bits (72), Expect = 0.12
Identities = 37/137 (27%), Positives = 55/137 (40%), Gaps = 32/137 (23%)
Query: 34 NNNKLRRLPHVFSRILQLPLRSDADVSIEEEPTCFRFVAETDSSLGHVETHTLHIH---- 89
NNN+ + FS + P + S+ E + A ++ + ET H+
Sbjct: 9 NNNRRSNIFDPFSLDVWDPFKELTSSSLSRENS-----AIVNARVDWRETPEAHVFKADL 63
Query: 90 PGVTK----------IVVRASHSLHFSLDDLHPDIW------------RFRLPESVVPEL 127
PG+ K V++ S H +D + D W RFRLPE+V +
Sbjct: 64 PGLKKEEVKVEIEEDSVLKISGERHVEKEDKN-DTWHRVERSSGQFTRRFRLPENVKMDQ 122
Query: 128 ATAVFVDGELIVTVPKA 144
A +G L VTVPKA
Sbjct: 123 VKAAMENGVLTVTVPKA 139
>At5g59720 heat shock protein 18
Length = 161
Score = 30.0 bits (66), Expect = 0.59
Identities = 18/44 (40%), Positives = 23/44 (51%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPKAFDDENTPEPIGGGGA 159
RFRLPE+ E A +G L V VPKA + + + I GA
Sbjct: 117 RFRLPENAKMEEVKATMENGVLTVVVPKAPEKKPQVKSIDISGA 160
>At3g46230 heat shock protein 17
Length = 156
Score = 30.0 bits (66), Expect = 0.59
Identities = 18/37 (48%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPKAFDDENTPE 152
RFRLPE+ E A +G L VTVPK E+ PE
Sbjct: 114 RFRLPENAKVEEVKASMENGVLSVTVPKV--QESKPE 148
>At1g78560 Unknown protein (T30F21.11)
Length = 401
Score = 29.6 bits (65), Expect = 0.77
Identities = 30/102 (29%), Positives = 42/102 (40%), Gaps = 17/102 (16%)
Query: 22 AETLLSMSGFSNNNNKLRRLPHVFSRILQLPLRSDADVSIEEEPTCFRFVAETDSSLGHV 81
A LL +SGF ++FSRIL + + S +SIE V +S LG V
Sbjct: 315 ASCLLHISGFL--------FGYLFSRILGIDVASSRTISIE--------VGMQNSVLG-V 357
Query: 82 ETHTLHIHPGVTKIVVRASHSLHFSLDDLHPDIWRFRLPESV 123
T H +T + S H L + IWR P+ +
Sbjct: 358 VLATQHFGNPLTAVPCAVSSVCHSILGSVLAGIWRRSAPKQL 399
>At1g53540 17.6 kDa heat shock protein (AA 1-156)
Length = 157
Score = 28.9 bits (63), Expect = 1.3
Identities = 18/37 (48%), Positives = 20/37 (53%), Gaps = 2/37 (5%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPKAFDDENTPE 152
RFRLPE+ E A +G L VTVPK E PE
Sbjct: 115 RFRLPENAKMEEIKASMENGVLSVTVPKV--PEKKPE 149
>At5g39760 unknown protein
Length = 334
Score = 28.5 bits (62), Expect = 1.7
Identities = 12/21 (57%), Positives = 16/21 (76%)
Query: 18 PLSQAETLLSMSGFSNNNNKL 38
P+S + LLS+SG +NNNN L
Sbjct: 154 PISSSYMLLSLSGTNNNNNNL 174
>At1g59860 unknown protein
Length = 155
Score = 28.5 bits (62), Expect = 1.7
Identities = 14/28 (50%), Positives = 18/28 (64%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPK 143
+FRLPE+V + A +G L VTVPK
Sbjct: 111 KFRLPENVKMDQVKASMENGVLTVTVPK 138
>At4g24560 putative protein
Length = 1008
Score = 28.1 bits (61), Expect = 2.2
Identities = 17/54 (31%), Positives = 25/54 (45%), Gaps = 2/54 (3%)
Query: 56 DADVSIEEEPTCFRFVAETDSSLGHVETHTLHIHPGVTKIVVRASHSLHFSLDD 109
DA VS+E E TCF + + S L + +H+ V + S+H DD
Sbjct: 231 DAHVSVEPEDTCFSTIEDAPSKL--LSPKFVHLVESVDNLANLPKLSVHKPEDD 282
>At5g12020 heat shock protein 17.6-II
Length = 155
Score = 27.3 bits (59), Expect = 3.8
Identities = 31/119 (26%), Positives = 52/119 (43%), Gaps = 10/119 (8%)
Query: 33 NNNNKLRRLP-HVFSRILQLPLRSDADVSIEEEPTCFRFVAETDSSLG-----HVETHTL 86
+NN K R P V+ R + + ADV E P + FV + G VE +
Sbjct: 22 HNNEKTRNNPSRVYMRDAKAMAATPADVI--EHPNAYAFVVDMPGIKGDEIKVQVENDNV 79
Query: 87 HIHPGVTKIVVRASHSLHFSLDD--LHPDIWRFRLPESVVPELATAVFVDGELIVTVPK 143
+ G + + + + + + + + +F+LPE+ + +AV DG L VTV K
Sbjct: 80 LVVSGERQRENKENEGVKYVRMERRMGKFMRKFQLPENADLDKISAVCHDGVLKVTVQK 138
>At4g21870 heat shock protein - like
Length = 134
Score = 27.3 bits (59), Expect = 3.8
Identities = 17/45 (37%), Positives = 23/45 (50%), Gaps = 6/45 (13%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPK------AFDDENTPEPI 154
+FRLPES+ +A + DG L V VPK D + PE +
Sbjct: 83 KFRLPESIDMIGISAGYEDGVLTVIVPKRIMTRRLIDPSDVPESL 127
>At1g07400 heat shock like protein
Length = 157
Score = 27.3 bits (59), Expect = 3.8
Identities = 13/28 (46%), Positives = 18/28 (63%)
Query: 116 RFRLPESVVPELATAVFVDGELIVTVPK 143
+F+LPE+V + A +G L VTVPK
Sbjct: 113 KFKLPENVKMDQVKASMENGVLTVTVPK 140
>At5g61290 unknown protein
Length = 461
Score = 26.9 bits (58), Expect = 5.0
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 13/60 (21%)
Query: 80 HVETHTLHIHPGVTKIVVRASHSLHFSLDDLHPDIWRFRLPESVVPELATAVFVDGELIV 139
H+ T +L I PG++K V+ +LH LHP I + E +F DG IV
Sbjct: 240 HLSTKSLDIPPGLSK-VIEKHQNLH-----LHPQI-------ESLEEDGRVIFEDGSCIV 286
>At3g13445 transcription initiation factor TFIID-1 (TATA
sequence-binding protein 1)
Length = 200
Score = 26.6 bits (57), Expect = 6.5
Identities = 10/44 (22%), Positives = 23/44 (51%)
Query: 104 HFSLDDLHPDIWRFRLPESVVPELATAVFVDGELIVTVPKAFDD 147
H + P+++ + VP++ +FV G++++T K D+
Sbjct: 137 HAAFSSYEPELFPGLIYRMKVPKIVLLIFVSGKIVITGAKMRDE 180
>At2g17140 hypothetical protein
Length = 903
Score = 26.6 bits (57), Expect = 6.5
Identities = 25/111 (22%), Positives = 41/111 (36%), Gaps = 19/111 (17%)
Query: 34 NNNKLRRLPHVFSRILQLPLRSDADVSIEEEPTCFRFVAETDSSLGHVETHTLHIHPGVT 93
N N R +F RI P +S++ PT R + E H L + +
Sbjct: 12 NTNNPRLAWRIFKRIFSSPSEESHGISLDATPTIARILVRAKMHEEIQELHNLILSSSIQ 71
Query: 94 K-----------IVVRASHSLHFSLDDLHPDIWRFRLPESVVPELATAVFV 133
K I +++H +D P +F+L S PE +V++
Sbjct: 72 KTKLSSLLSVVSIFAKSNH-----IDKAFP---QFQLVRSRFPENKPSVYL 114
>At2g25370 hypothetical protein
Length = 546
Score = 26.2 bits (56), Expect = 8.5
Identities = 14/36 (38%), Positives = 17/36 (46%)
Query: 54 RSDADVSIEEEPTCFRFVAETDSSLGHVETHTLHIH 89
R+ DV+ E+ TC F ETD V LH H
Sbjct: 291 RNSVDVNAEQGETCGIFFEETDVEHMFVTEKCLHRH 326
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.136 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,935,019
Number of Sequences: 26719
Number of extensions: 157137
Number of successful extensions: 399
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 380
Number of HSP's gapped (non-prelim): 17
length of query: 165
length of database: 11,318,596
effective HSP length: 92
effective length of query: 73
effective length of database: 8,860,448
effective search space: 646812704
effective search space used: 646812704
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC135320.11