BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134521.7 + phase: 0
(183 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52780 unknown protein 115 1e-26
At4g19100 putative protein 93 7e-20
At1g62970 unknown protein 32 0.19
At3g06130 unknown protein 32 0.25
At5g63850 amino acid transporter AAP4 (pir||S51169) 31 0.33
At1g22850 unknown protein 30 0.95
At4g37130 nucleoporin-like protein 29 1.6
At5g09220 amino acid transport protein AAP2 28 2.1
At3g19020 hypothetical protein 28 2.1
At1g77380 amino acid permease AAP3 28 2.1
At2g22520 hypothetical protein 28 2.8
At1g53510 mitogen-activated 28 2.8
At5g28646 putative protein 28 3.6
At4g08630 hypothetical protein 28 3.6
At1g44100 amino acid permease AAP5 27 4.7
At1g08350 endosomal like protein 27 4.7
At5g53190 MtN3 protein-like 27 6.1
At1g17360 COP1-Interacting Protein 7, putative 27 6.1
At5g55200 chaperone GrpE-like protein 27 8.0
At3g62780 shock protein SRC2-like 27 8.0
>At5g52780 unknown protein
Length = 168
Score = 115 bits (288), Expect = 1e-26
Identities = 58/134 (43%), Positives = 84/134 (62%), Gaps = 8/134 (5%)
Query: 45 KPQASAKGFSNTRPPSTNKAKDDVPIKKNPNNKNDDDDIPKEVMYRIIGRILFSTLVPMA 104
K A KGF ++R D P DD IP+EV R++GRI+ S P+
Sbjct: 42 KLHAGPKGFQSSRTSEKPGRPDPDP--------EDDPPIPQEVFERMMGRIVVSVGTPLG 93
Query: 105 LGLSFLHLYGELKDRHIFNAPLWMPFVTTLVFFGASGLGIAYGVLSTSLDAEREGSLLGF 164
LG++ L + LKDR++++ PLW+P++TTLV FG+S LGIAYG LST+LD + SL G
Sbjct: 94 LGVAILKVLEVLKDRNVWDVPLWVPYLTTLVTFGSSALGIAYGSLSTNLDPAKTNSLFGL 153
Query: 165 EEVEKNWDEMWQQE 178
+E ++NW EMW+++
Sbjct: 154 KEAKENWVEMWKED 167
>At4g19100 putative protein
Length = 214
Score = 93.2 bits (230), Expect = 7e-20
Identities = 52/143 (36%), Positives = 77/143 (53%), Gaps = 18/143 (12%)
Query: 49 SAKGFSNTRPPSTNKAKDDVPIKKNPNNKNDDDD---------------IPKEVMYRIIG 93
S KGF PP K P N ++++DDD+ IP+ V R+I
Sbjct: 66 SPKGFG---PPPKKTKKSKKPKPGNQSDEDDDDEDEDDDDEEDERERGVIPEIVTNRMIS 122
Query: 94 RILFSTLVPMALGLSFLHLYGELKDRHIFNAPLWMPFVTTLVFFGASGLGIAYGVLSTSL 153
R+ F+ +P+ +GL F + LK + P W+PF+ + VFFG + G++YG++S+S
Sbjct: 123 RMGFTVGLPLFIGLLFFPFFYYLKVGLKVDVPTWVPFIVSFVFFGTALAGVSYGIVSSSW 182
Query: 154 DAEREGSLLGFEEVEKNWDEMWQ 176
D REGSLLG+ E +KNW WQ
Sbjct: 183 DPLREGSLLGWNEAKKNWPVFWQ 205
>At1g62970 unknown protein
Length = 825
Score = 32.0 bits (71), Expect = 0.19
Identities = 22/70 (31%), Positives = 34/70 (48%), Gaps = 16/70 (22%)
Query: 10 PILQSSTQS---PICKPQK----FPFSQPNTSKQ---------LNYQFTTLKPQASAKGF 53
P+ Q S+ S P+ +PQ FP SQP + Q F +P A++K F
Sbjct: 454 PVSQPSSNSKPFPVSQPQPASNPFPVSQPRPNSQPFSMSQPSSTARPFPASQPPAASKSF 513
Query: 54 SNTRPPSTNK 63
++PP+T+K
Sbjct: 514 PISQPPTTSK 523
>At3g06130 unknown protein
Length = 473
Score = 31.6 bits (70), Expect = 0.25
Identities = 19/57 (33%), Positives = 28/57 (48%), Gaps = 3/57 (5%)
Query: 31 PNTSKQLNYQ-FTTLKPQASAKGFSNTRPPSTNKAKDDVPIKKNPNNKNDDDDIPKE 86
P +QLN Q L+ KGF + + P + K VP+ KN N K D+P++
Sbjct: 135 PKMGQQLNPQHLQQLQQLQKMKGFQDLKLPP--QLKGSVPVNKNQNQKGVKFDVPED 189
>At5g63850 amino acid transporter AAP4 (pir||S51169)
Length = 466
Score = 31.2 bits (69), Expect = 0.33
Identities = 17/65 (26%), Positives = 32/65 (49%), Gaps = 8/65 (12%)
Query: 112 LYGELKDRHIFNAPLWMPFVTTLVFFGASGLGIAYGVLSTSLDAEREGSLLGFE-----E 166
L ++KD F+ W+ V ++ F S +G+A G++ + + +GSL G +
Sbjct: 164 LLSQIKD---FDQIWWLSIVAAIMSFTYSAIGLALGIIQVAANGVVKGSLTGISIGAVTQ 220
Query: 167 VEKNW 171
+K W
Sbjct: 221 TQKIW 225
>At1g22850 unknown protein
Length = 344
Score = 29.6 bits (65), Expect = 0.95
Identities = 26/90 (28%), Positives = 41/90 (44%), Gaps = 11/90 (12%)
Query: 3 TLVFASNPILQSSTQSPICKPQKFPFSQPNTSKQLNYQFTTLKPQASAKGFSNTRP---P 59
TL +S+ L + S C P P++S +L +F LKP +S K + P
Sbjct: 9 TLRSSSSSSLPFTCNSRFCSPPP-----PSSSFRLYKRFHFLKPCSSLKQTKKKKQQSLP 63
Query: 60 STNKAKDDVPI---KKNPNNKNDDDDIPKE 86
ST + K+ N++ND+DD+ E
Sbjct: 64 STAPPPQSLRWFFNSKSTNDENDEDDVKSE 93
>At4g37130 nucleoporin-like protein
Length = 513
Score = 28.9 bits (63), Expect = 1.6
Identities = 17/53 (32%), Positives = 26/53 (48%), Gaps = 3/53 (5%)
Query: 10 PILQSSTQSPICKPQKFPFSQPNTSKQLNYQFTTLKPQASAKGFSNTRPPSTN 62
P Q T P+ + Q+ QP +Q N F+ +PQ + FS ++P TN
Sbjct: 5 PPQQQQTPQPLFQTQQTSLFQP---QQTNSIFSQSQPQQTNSIFSQSQPQQTN 54
>At5g09220 amino acid transport protein AAP2
Length = 493
Score = 28.5 bits (62), Expect = 2.1
Identities = 14/55 (25%), Positives = 27/55 (48%), Gaps = 5/55 (9%)
Query: 122 FNAPLWMPFVTTLVFFGASGLGIAYGVLSTSLDAEREGSLLGFE-----EVEKNW 171
F+ W+ V ++ F S +G+A G++ + + +GSL G + +K W
Sbjct: 198 FDQIWWISIVAAVMSFTYSAIGLALGIVQVAANGVFKGSLTGISIGTVTQTQKIW 252
>At3g19020 hypothetical protein
Length = 951
Score = 28.5 bits (62), Expect = 2.1
Identities = 18/70 (25%), Positives = 31/70 (43%), Gaps = 5/70 (7%)
Query: 15 STQSPICK--PQKFPFSQPNTSKQLNYQFTTLKPQASAKGFSNTRPPSTNKAKDDVPIKK 72
S SP+ K P P S+P + ++ ++ ++ PPS+N + D +
Sbjct: 862 SNHSPVFKSSPAPSPDSEPEVEAPVPSSEPEVEAPKQSEATPSSSPPSSNPSPD---VTA 918
Query: 73 NPNNKNDDDD 82
P+ NDD D
Sbjct: 919 PPSEDNDDGD 928
>At1g77380 amino acid permease AAP3
Length = 476
Score = 28.5 bits (62), Expect = 2.1
Identities = 16/65 (24%), Positives = 31/65 (47%), Gaps = 8/65 (12%)
Query: 112 LYGELKDRHIFNAPLWMPFVTTLVFFGASGLGIAYGVLSTSLDAEREGSLLGFE-----E 166
L+ ++ D F+ W+ + ++ F S G+A G+ ++ + +GSL G E
Sbjct: 175 LFSQIPD---FDQLWWLSILAAVMSFTYSSAGLALGIAQVVVNGKVKGSLTGISIGAVTE 231
Query: 167 VEKNW 171
+K W
Sbjct: 232 TQKIW 236
>At2g22520 hypothetical protein
Length = 185
Score = 28.1 bits (61), Expect = 2.8
Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 6/54 (11%)
Query: 21 CKPQKFPFSQPNTSK-QLNYQFTTLKPQASAKGFSNT-----RPPSTNKAKDDV 68
C PQKFPF S+ + + Q T LKP ++ ++ +P +++K DV
Sbjct: 52 CYPQKFPFKPSKPSQIKADVQETLLKPSKPSQSKADVQETLLKPSKPSQSKADV 105
>At1g53510 mitogen-activated
Length = 603
Score = 28.1 bits (61), Expect = 2.8
Identities = 19/68 (27%), Positives = 29/68 (41%), Gaps = 11/68 (16%)
Query: 13 QSSTQSPICKPQKFPFSQPNT-----SKQLNYQFTTLKPQASAKGFSNTRPPSTNKAKDD 67
+S+ SP C + +PNT ++ + KPQ FS T PP+ + D
Sbjct: 485 RSAVSSPHC------YFRPNTMTNPENRNIEASSFPPKPQNPVHQFSPTEPPAATTNQAD 538
Query: 68 VPIKKNPN 75
V +PN
Sbjct: 539 VETMNHPN 546
>At5g28646 putative protein
Length = 293
Score = 27.7 bits (60), Expect = 3.6
Identities = 14/36 (38%), Positives = 20/36 (54%), Gaps = 2/36 (5%)
Query: 2 KTLVFASNPILQSSTQSPICKPQ--KFPFSQPNTSK 35
K L F +NP+ Q P KP+ KFP ++P + K
Sbjct: 112 KNLKFKANPVPDFYYQRPPVKPELKKFPLTRPKSPK 147
>At4g08630 hypothetical protein
Length = 779
Score = 27.7 bits (60), Expect = 3.6
Identities = 21/86 (24%), Positives = 33/86 (37%), Gaps = 10/86 (11%)
Query: 8 SNPILQSSTQSPICKPQKFPFSQ----PNTSKQLNYQFTTLKPQASAKGFSNTRPPSTNK 63
S+P ++P+ + Q+ Q N + T+ +P G RP S
Sbjct: 94 SHPSRSPVIRNPMARRQQMATPQLVDEDNEDDDFSVDVTSSRPSIGLPGGRAMRPQSPVM 153
Query: 64 AKDDVPIKKNPNNK------NDDDDI 83
K P + P N+ NDDDD+
Sbjct: 154 TKIAPPRRTQPTNEGNKNDGNDDDDV 179
>At1g44100 amino acid permease AAP5
Length = 480
Score = 27.3 bits (59), Expect = 4.7
Identities = 13/42 (30%), Positives = 21/42 (49%)
Query: 122 FNAPLWMPFVTTLVFFGASGLGIAYGVLSTSLDAEREGSLLG 163
F+ W+ V ++ F S +G+ GV + E +GSL G
Sbjct: 180 FDQLWWLSIVAAVMSFAYSAIGLGLGVSKVVENKEIKGSLTG 221
>At1g08350 endosomal like protein
Length = 589
Score = 27.3 bits (59), Expect = 4.7
Identities = 26/95 (27%), Positives = 42/95 (43%), Gaps = 16/95 (16%)
Query: 52 GFSNTRPPSTNKAKDDVPIKKNPNNKNDDDDIPKEVMYRI-IGRILFSTLVPM-ALGLSF 109
G +PPS +K+NP +IP + YR + ++ VP A+ L +
Sbjct: 421 GLLEFQPPSA--------VKRNPR------EIPPQNWYRRKLYQVFLGGFVPFSAVVLEW 466
Query: 110 LHLYGELKDRHIFNAPLWMPFVTTLVFFGASGLGI 144
LY L I+ +P M F ++ F +S +GI
Sbjct: 467 HQLYASLWGFKIYTSPGIMLFTFIVLIFLSSSVGI 501
>At5g53190 MtN3 protein-like
Length = 261
Score = 26.9 bits (58), Expect = 6.1
Identities = 13/27 (48%), Positives = 17/27 (62%)
Query: 127 WMPFVTTLVFFGASGLGIAYGVLSTSL 153
+MPF + F AS L +AYG+LS L
Sbjct: 162 YMPFYLSFFSFLASSLWLAYGLLSHDL 188
>At1g17360 COP1-Interacting Protein 7, putative
Length = 1061
Score = 26.9 bits (58), Expect = 6.1
Identities = 17/63 (26%), Positives = 31/63 (48%), Gaps = 3/63 (4%)
Query: 23 PQKFPFSQPNTSKQLNYQFTTLKPQASAKGFSNTRPPSTNKAKDDV--PIKKNPNNKNDD 80
P+K + + +++ FT ++ + S G + P ST + D P + N+K DD
Sbjct: 291 PEKSVLRRWSIVSDMSFDFT-MENKKSDSGSNEEGPLSTPSSIPDATFPKESEENSKKDD 349
Query: 81 DDI 83
DD+
Sbjct: 350 DDV 352
>At5g55200 chaperone GrpE-like protein
Length = 302
Score = 26.6 bits (57), Expect = 8.0
Identities = 10/39 (25%), Positives = 23/39 (58%)
Query: 54 SNTRPPSTNKAKDDVPIKKNPNNKNDDDDIPKEVMYRII 92
SNT P T + ++V + K + + D DD+ ++ + +++
Sbjct: 85 SNTEVPKTGETSENVEVGKATDAEIDFDDLSRDDLVKLV 123
>At3g62780 shock protein SRC2-like
Length = 298
Score = 26.6 bits (57), Expect = 8.0
Identities = 15/52 (28%), Positives = 20/52 (37%)
Query: 10 PILQSSTQSPICKPQKFPFSQPNTSKQLNYQFTTLKPQASAKGFSNTRPPST 61
P+ Q P+C P +P S S YQ +G+S PP T
Sbjct: 234 PLAQPEILPPVCFPGLYPPSNMPGSPPTMYQSIFPGLSCPPEGYSTVAPPLT 285
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.134 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,479,278
Number of Sequences: 26719
Number of extensions: 195891
Number of successful extensions: 670
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 654
Number of HSP's gapped (non-prelim): 29
length of query: 183
length of database: 11,318,596
effective HSP length: 93
effective length of query: 90
effective length of database: 8,833,729
effective search space: 795035610
effective search space used: 795035610
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC134521.7