BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127428.6 - phase: 0 /pseudo
(322 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g30300 hypothetical protein 395 e-110
At4g03610 putative hydrolase 298 3e-81
At3g13800 hydrolase like protein 143 1e-34
At5g63420 putative protein 30 1.3
At1g16500 unknown protein 29 3.0
At1g53345 unknown protein 29 3.9
At1g18370 AtNACK1 kinesin-like protein (AtNACK1) 29 3.9
At3g23600 unknown protein 28 5.1
At1g76150 unknown protein 28 8.7
At1g63580 hypothetical protein 28 8.7
>At1g30300 hypothetical protein
Length = 323
Score = 395 bits (1016), Expect = e-110
Identities = 189/256 (73%), Positives = 219/256 (84%), Gaps = 3/256 (1%)
Query: 1 MATSNGTDDGTPPSE---SALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKN 57
M +S +++G+ S+ SALIFLGTGCSS VPN +CLI SD PC VC+QSLSIPPEKN
Sbjct: 1 MESSIPSENGSVTSDRDRSALIFLGTGCSSAVPNAMCLIQRSDNPCYVCSQSLSIPPEKN 60
Query: 58 PNYRCNTSMLIDYCGSGSNHNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAV 117
PNYR NTS+LIDYC S H YI IDVGKTFRE VLRWF H+IP++DSIILTHEHADAV
Sbjct: 61 PNYRGNTSLLIDYCQSDGKHKYIQIDVGKTFREQVLRWFTLHKIPQVDSIILTHEHADAV 120
Query: 118 LGLDDVRAVQPFSPTNDIDPTPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWN 177
LGLDD+R+VQPFSPTNDIDPTPI++SQ++M+S+ KFPYLVQK+ KEGQE+RRVAQ+ W
Sbjct: 121 LGLDDIRSVQPFSPTNDIDPTPIFVSQYAMESLAVKFPYLVQKKLKEGQEVRRVAQLDWR 180
Query: 178 IITDDCNQPFFASGLKFTPLPVMHGEDYICLGFLFGEKSRVAYISDVSRIPASTEYVISK 237
+I +DC +PF ASGL FTPLPVMHGEDY+CLGFLFGEKSRVAYISDVSR P +TEY ISK
Sbjct: 181 VIEEDCEKPFVASGLSFTPLPVMHGEDYVCLGFLFGEKSRVAYISDVSRFPPNTEYAISK 240
Query: 238 SGAGQLDLLILDSLYR 253
SG GQLDLLILD+LY+
Sbjct: 241 SGGGQLDLLILDTLYK 256
>At4g03610 putative hydrolase
Length = 290
Score = 298 bits (762), Expect = 3e-81
Identities = 156/253 (61%), Positives = 185/253 (72%), Gaps = 25/253 (9%)
Query: 1 MATSNGTDDGTPPSESALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNY 60
M + DDGT ALIFLGTGCS VP+ CL+ PSDPPC VC+QSLS+ P NPNY
Sbjct: 1 MEKNQSDDDGT-----ALIFLGTGCSGAVPDFRCLLQPSDPPCHVCSQSLSLLPHLNPNY 55
Query: 61 RCNTSMLIDYCGSGSN--HNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVL 118
RCNTS+LIDYC + H YILIDVGK+FRE IILTHEHADAV
Sbjct: 56 RCNTSLLIDYCSKEEDGRHKYILIDVGKSFRE---------------QIILTHEHADAVH 100
Query: 119 GLDDVRAVQPFSPTN-DIDPTPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWN 177
GLD++R++QP T D DP P++LSQ +M+SI +FPYLV+K+ KE RRV+ + W
Sbjct: 101 GLDEIRSLQPRGATIVDTDPLPVFLSQFTMESIATRFPYLVEKKVKEVP--RRVSLLDWK 158
Query: 178 IITDDCNQPFFASGLKFTPLPVMHGEDYICLGFLFGEKSRVAYISDVSRIPASTEYVISK 237
I ++C++PF ASGL FTPLPVMHGEDYI LGFLFG+KS+VAYISDVSRIP STEY ISK
Sbjct: 159 NIEENCDEPFAASGLSFTPLPVMHGEDYIALGFLFGDKSKVAYISDVSRIPPSTEYAISK 218
Query: 238 SGAGQLDLLILDS 250
+GAGQLDLLILD+
Sbjct: 219 AGAGQLDLLILDT 231
>At3g13800 hydrolase like protein
Length = 361
Score = 143 bits (361), Expect = 1e-34
Identities = 96/237 (40%), Positives = 132/237 (55%), Gaps = 23/237 (9%)
Query: 16 SALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNTSMLIDYCGSGS 75
S ++F+GTG S +P V CL NP CSVC ++ N N R NTS+L+ Y
Sbjct: 78 SEIVFMGTGTSEGIPRVSCLTNPLKT-CSVCTKATE---PGNRNRRLNTSILVRYIRPSG 133
Query: 76 NHNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGLDDVRAVQPFSPTNDI 135
N ILID GK F + LRWF + +D++++TH HADA+ GLDD+R TN++
Sbjct: 134 TSN-ILIDCGKFFYHSALRWFPTFGLRTLDAVVITHSHADAIGGLDDLR-----DWTNNV 187
Query: 136 DP-TPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWNIITDDCNQPFFASGLKF 194
P PIY + ++ +++ YLV V+++ + +I +D QPF + LK
Sbjct: 188 QPHIPIYTAIRDLEVMKKTHYYLVDTSVIIPG--AAVSELEFKVIHED--QPFVVNDLKI 243
Query: 195 TPLPVMHGEDYICLGFLFGEKSRVAYISDVSRIPASTEYVISKSGAGQLDLLILDSL 251
TPLPV HG +Y LGF FG V YISDVS IP T Y + K DLLI+D+L
Sbjct: 244 TPLPVWHGSNYRSLGFRFG---NVCYISDVSDIPEET-YPLLK----DCDLLIMDAL 292
>At5g63420 putative protein
Length = 528
Score = 30.4 bits (67), Expect = 1.3
Identities = 25/90 (27%), Positives = 41/90 (44%), Gaps = 18/90 (20%)
Query: 74 GSNHNYILIDVGKTFRE---------TVLRWFVHHRIPKIDSIILTHEHADAVLGLDDVR 124
G+ YILID G F + F+ KI+++++TH H D + L V
Sbjct: 129 GNYDRYILIDAGIMFPDYDEPGIQKIMPDTGFIRRWKHKIEAVVITHGHEDHIGALPWVI 188
Query: 125 AVQPFSPTNDIDP-TPIYLSQHSMDSIEEK 153
+DP TPI+ S +M+ I+++
Sbjct: 189 PA--------LDPNTPIFASSFTMELIKKR 210
>At1g16500 unknown protein
Length = 259
Score = 29.3 bits (64), Expect = 3.0
Identities = 18/62 (29%), Positives = 25/62 (40%), Gaps = 2/62 (3%)
Query: 5 NGTDDGTPPSESALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNT 64
+G DGT + A + GC + V N +P C C + +P K P N
Sbjct: 200 DGVYDGTASATVAASQVAAGCPGCLSYVFVAKN--NPKCPRCHSFVPLPAMKKPKIDLNI 257
Query: 65 SM 66
SM
Sbjct: 258 SM 259
>At1g53345 unknown protein
Length = 325
Score = 28.9 bits (63), Expect = 3.9
Identities = 11/28 (39%), Positives = 17/28 (60%)
Query: 96 FVHHRIPKIDSIILTHEHADAVLGLDDV 123
FVH PK+D++++ H A+ L DV
Sbjct: 72 FVHQVSPKVDNVVILDHHKTAIDSLGDV 99
>At1g18370 AtNACK1 kinesin-like protein (AtNACK1)
Length = 974
Score = 28.9 bits (63), Expect = 3.9
Identities = 16/54 (29%), Positives = 25/54 (45%)
Query: 128 PFSPTNDIDPTPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWNIITD 181
P +P + +D TP S S EEK V+ + +E+ Q+AW + D
Sbjct: 6 PGTPVSKMDRTPAVTPGGSSRSREEKIVVTVRLRPMNKRELLAKDQVAWECVND 59
>At3g23600 unknown protein
Length = 239
Score = 28.5 bits (62), Expect = 5.1
Identities = 12/49 (24%), Positives = 23/49 (46%)
Query: 128 PFSPTNDIDPTPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAW 176
P++P+N P P+++ H D E +++ + +G A M W
Sbjct: 80 PYNPSNQDRPIPVWIKDHGCDKGFENTKPVLETIKNKGITAIGAAGMCW 128
>At1g76150 unknown protein
Length = 309
Score = 27.7 bits (60), Expect = 8.7
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 6/64 (9%)
Query: 144 QHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWNIIT-DDCNQPFFASGLKFTPLPVMHG 202
Q ++DS E KF Y + GQ++ +V ++ T + G K+ P ++HG
Sbjct: 42 QDAVDSDELKFVY-----HRNGQDLIQVLPTFASLFTLGSLTEGLDLPGFKYDPSLLLHG 96
Query: 203 EDYI 206
+ YI
Sbjct: 97 QQYI 100
>At1g63580 hypothetical protein
Length = 273
Score = 27.7 bits (60), Expect = 8.7
Identities = 34/130 (26%), Positives = 54/130 (41%), Gaps = 26/130 (20%)
Query: 33 LCLINPSDPPCSVCAQSLSIPPEKNPNYRCNTSMLIDYC---GSGSNHNYILIDVGKTF- 88
LC+ N S CS C S ++ +K+ + M D C S ++ ++ D TF
Sbjct: 75 LCIGNISKTSCSNCVHSATLEMDKSCESHDTSFMFSDECMVRYSDNSFFSLVEDSPATFS 134
Query: 89 --RETVLRW--FVHHRIP-KIDSIIL---------------THEHADAVLGLDDVRAVQP 128
+ L + F + +P K+D +IL EH V G D+ A+
Sbjct: 135 YSQNDSLSYPQFYNQTLPGKLDELILKAPSSFSSPVPYFVEDKEHVTQVEGSYDLEAMAQ 194
Query: 129 FSPTNDIDPT 138
SP D+DP+
Sbjct: 195 CSP--DLDPS 202
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.332 0.145 0.468
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,551,205
Number of Sequences: 26719
Number of extensions: 328577
Number of successful extensions: 860
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 847
Number of HSP's gapped (non-prelim): 10
length of query: 322
length of database: 11,318,596
effective HSP length: 99
effective length of query: 223
effective length of database: 8,673,415
effective search space: 1934171545
effective search space used: 1934171545
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 60 (27.7 bits)
Medicago: description of AC127428.6