BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127169.10 + phase: 1 /pseudo/partial
(367 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g65610 hypothetical protein 72 5e-13
At5g49720 cellulase homolog OR16pep precursor (pir||S71215) 56 4e-08
At4g24260 endo-1,4-beta-glucanase like protein 53 3e-07
At4g09740 cellulase-like protein 40 0.002
At1g75680 endo-beta-1,4-glucanase, putative 40 0.003
At4g23560 putative cellulase 39 0.003
At3g43860 cellulase like protein 35 0.065
At2g44560 putative glucanase 35 0.085
At1g22880 putative endo-1,4-beta-D-glucanase 34 0.11
At1g19940 putative protein 34 0.14
At4g39000 endo-1,4-beta-glucanase like protein 33 0.32
At1g71380 beta-glucanase 33 0.32
At1g02800 endo-1,4-beta-glucanase precursor, putative 33 0.32
At4g02290 putative endo-1,4-beta glucanase 32 0.55
At2g32990 putative glucanse 32 0.55
At4g11050 glucanase like protein 30 1.6
At2g44570 putative glucanase 30 1.6
At4g39010 putative endo-1,4-beta-glucanase 30 2.1
At4g38990 putative endo-1,4-beta-glucanase 30 2.1
At2g44550 putative glucanase 30 2.7
>At1g65610 hypothetical protein
Length = 623
Score = 72.0 bits (175), Expect = 5e-13
Identities = 32/67 (47%), Positives = 46/67 (67%), Gaps = 9/67 (13%)
Query: 44 RQNTVNRYDHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGSE---------KPE 94
+ ++ YDH R+++KWGTDYLLLTF++SA+ ++ I+ +VGG L+ SE KPE
Sbjct: 195 KYKAIDEYDHMRDVLKWGTDYLLLTFNNSATRLDHIYTQVGGGLRDSESPDDIYCWQKPE 254
Query: 95 DMEYPRP 101
DM Y RP
Sbjct: 255 DMSYDRP 261
Score = 51.2 bits (121), Expect = 9e-07
Identities = 31/58 (53%), Positives = 39/58 (66%), Gaps = 4/58 (6%)
Query: 311 YDLRNNNSYTEPTLAGNAGLVAALISLTSTSTIGSGFDTDHTIFNQTRQ-YRPQNLPP 367
+DLRNN + +EPTL+GNAGLVAAL+SLTS+ G D +T+FN Y P PP
Sbjct: 564 HDLRNNYNASEPTLSGNAGLVAALVSLTSSG--GQQID-KNTMFNSVPPLYSPTPPPP 618
>At5g49720 cellulase homolog OR16pep precursor (pir||S71215)
Length = 621
Score = 55.8 bits (133), Expect = 4e-08
Identities = 31/72 (43%), Positives = 43/72 (59%), Gaps = 11/72 (15%)
Query: 53 HARELIKWGTDYLLLTFDSSASEINQIHAKVG------GSLKGSE-----KPEDMEYPRP 101
H +ELIKWGTDY L TF+S+A I+ + ++VG G+ ++ +PEDM+Y RP
Sbjct: 199 HVKELIKWGTDYFLKTFNSTADSIDDLVSQVGSGNTDDGNTDPNDHYCWMRPEDMDYKRP 258
Query: 102 TTTI*SEGPDLA 113
TT DLA
Sbjct: 259 VTTCNGGCSDLA 270
Score = 48.1 bits (113), Expect = 7e-06
Identities = 27/56 (48%), Positives = 34/56 (60%), Gaps = 1/56 (1%)
Query: 312 DLRNNNSYTEPTLAGNAGLVAALISLTSTSTIGSGFDTDHTIFNQTRQYRPQNLPP 367
D+R N +YTEPTLAGNAGLVAAL++L+ D +TIF+ P PP
Sbjct: 561 DVRMNYNYTEPTLAGNAGLVAALVALSGEEEATGKID-KNTIFSAVPPLFPTPPPP 615
>At4g24260 endo-1,4-beta-glucanase like protein
Length = 620
Score = 52.8 bits (125), Expect = 3e-07
Identities = 31/85 (36%), Positives = 44/85 (51%), Gaps = 11/85 (12%)
Query: 40 QLNIRQNTVNRYDHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSL--KGSE------ 91
+ + + +H +ELIKWGTDY L TF+SSA I + +VG + +GSE
Sbjct: 187 EYSAKYQAAGELEHVKELIKWGTDYFLKTFNSSADNIYVMVEQVGSGVSGRGSELHNDHY 246
Query: 92 ---KPEDMEYPRPTTTI*SEGPDLA 113
+PED+ Y R + S DLA
Sbjct: 247 CWMRPEDIHYKRTVSQCYSSCSDLA 271
Score = 49.7 bits (117), Expect = 3e-06
Identities = 25/45 (55%), Positives = 35/45 (77%), Gaps = 2/45 (4%)
Query: 311 YDLRNNNSYTEPTLAGNAGLVAALISLTSTSTIGSGFDTDHTIFN 355
+D+R N +YTEPTLAGNAGLVAAL++L+ +G G D +T+F+
Sbjct: 560 HDIRTNYNYTEPTLAGNAGLVAALVALSGEKAVG-GID-KNTMFS 602
>At4g09740 cellulase-like protein
Length = 480
Score = 40.4 bits (93), Expect = 0.002
Identities = 25/66 (37%), Positives = 38/66 (56%), Gaps = 7/66 (10%)
Query: 48 VNRYDHARELIKWGTDYLLLTFDSSASEINQIHAKVG-GSLKGS--EKPEDMEYPRPTTT 104
VN+ + R IKWGT+++L S+ N ++ +VG G+ S E+PEDM+ PR +
Sbjct: 105 VNQLGYLRSTIKWGTNFILRAHTST----NMLYTQVGDGNSDHSCWERPEDMDTPRTLYS 160
Query: 105 I*SEGP 110
I S P
Sbjct: 161 ISSSSP 166
>At1g75680 endo-beta-1,4-glucanase, putative
Length = 525
Score = 39.7 bits (91), Expect = 0.003
Identities = 24/70 (34%), Positives = 37/70 (52%), Gaps = 7/70 (10%)
Query: 44 RQNTVNRYDHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGS---EKPEDMEYPR 100
+ N VN+ D A++ ++W TDYL+ S N ++ +VG E+PEDM+ R
Sbjct: 134 QMNAVNQLDPAKDSLRWITDYLIKAHPSD----NVLYIQVGDPKVDHPCWERPEDMKEKR 189
Query: 101 PTTTI*SEGP 110
P T I + P
Sbjct: 190 PLTKIDVDTP 199
>At4g23560 putative cellulase
Length = 479
Score = 39.3 bits (90), Expect = 0.003
Identities = 25/68 (36%), Positives = 38/68 (55%), Gaps = 7/68 (10%)
Query: 46 NTVNRYDHARELIKWGTDYLLLTFDSSASEINQIHAKVG-GSLKGS--EKPEDMEYPRPT 102
++VN+ + R IKWGTD++L S N ++ +VG G+ S E+PEDM+ R
Sbjct: 103 SSVNQLGYLRSTIKWGTDFILRAHTSP----NMLYTQVGDGNSDHSCWERPEDMDTSRTL 158
Query: 103 TTI*SEGP 110
+I S P
Sbjct: 159 YSISSSSP 166
>At3g43860 cellulase like protein
Length = 486
Score = 35.0 bits (79), Expect = 0.065
Identities = 21/62 (33%), Positives = 33/62 (52%), Gaps = 7/62 (11%)
Query: 52 DHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGSE---KPEDMEYPRPTTTI*SE 108
++AR I+WGTDY L AS N+++ +VG + +PE+M+ PR I +
Sbjct: 120 ENARAAIRWGTDYFL----KCASRKNRLYVQVGDPNADHQCWARPENMKTPRTVLEISDK 175
Query: 109 GP 110
P
Sbjct: 176 VP 177
>At2g44560 putative glucanase
Length = 491
Score = 34.7 bits (78), Expect = 0.085
Identities = 23/67 (34%), Positives = 36/67 (53%), Gaps = 9/67 (13%)
Query: 52 DHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGSE---KPEDMEYPRPTTTI*SE 108
++A +KW TD+L+ + E N ++ +VG E +PEDM PRP+ I ++
Sbjct: 119 ENALAALKWATDFLI----KAHPEPNVLYGQVGDGNSDHECWMRPEDMTTPRPSFRIDAQ 174
Query: 109 --GPDLA 113
G DLA
Sbjct: 175 HPGSDLA 181
>At1g22880 putative endo-1,4-beta-D-glucanase
Length = 484
Score = 34.3 bits (77), Expect = 0.11
Identities = 18/58 (31%), Positives = 29/58 (49%)
Query: 53 HARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGSEKPEDMEYPRPTTTI*SEGP 110
++R I+W TDYLL ++ ++ G K E+PEDM+ PR ++ P
Sbjct: 109 NSRVAIRWATDYLLKCARATPGKLYVGVGDPNGDHKCWERPEDMDTPRTVYSVSPSNP 166
>At1g19940 putative protein
Length = 515
Score = 33.9 bits (76), Expect = 0.14
Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 7/67 (10%)
Query: 47 TVNRYDHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSL---KGSEKPEDMEYPRPTT 103
++N DHA++ +KW TD+L+ S N ++ +VG + K ++PE M R T
Sbjct: 128 SLNLLDHAKDSLKWTTDFLINAHPSP----NVLYIQVGDPVTDHKCWDRPETMTRKRTLT 183
Query: 104 TI*SEGP 110
I ++ P
Sbjct: 184 KIDTKTP 190
>At4g39000 endo-1,4-beta-glucanase like protein
Length = 493
Score = 32.7 bits (73), Expect = 0.32
Identities = 18/50 (36%), Positives = 24/50 (48%)
Query: 51 YDHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGSEKPEDMEYPR 100
+ H +KW TDYLL T + I +V E+PEDM+ PR
Sbjct: 109 FRHNLVAVKWATDYLLKTVSQLPNRIFVHVGEVQPDHDCWERPEDMDTPR 158
>At1g71380 beta-glucanase
Length = 484
Score = 32.7 bits (73), Expect = 0.32
Identities = 18/59 (30%), Positives = 30/59 (50%)
Query: 52 DHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGSEKPEDMEYPRPTTTI*SEGP 110
++AR I+W TDYLL ++ ++ K E+PEDM+ PR ++ + P
Sbjct: 108 ENARVNIRWATDYLLKCARATPGKLYVGVGDPNVDHKCWERPEDMDTPRTVYSVSASNP 166
>At1g02800 endo-1,4-beta-glucanase precursor, putative
Length = 501
Score = 32.7 bits (73), Expect = 0.32
Identities = 19/61 (31%), Positives = 31/61 (50%), Gaps = 7/61 (11%)
Query: 53 HARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGS---EKPEDMEYPRPTTTI*SEG 109
+A++ I+W TD+LL + S + I+ +VG E+PEDM+ PR +
Sbjct: 128 NAKDAIRWATDFLL----KATSHPDTIYVQVGDPNMDHACWERPEDMDTPRSVFKVDKNN 183
Query: 110 P 110
P
Sbjct: 184 P 184
>At4g02290 putative endo-1,4-beta glucanase
Length = 516
Score = 32.0 bits (71), Expect = 0.55
Identities = 19/51 (37%), Positives = 30/51 (58%), Gaps = 7/51 (13%)
Query: 53 HARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGS---EKPEDMEYPR 100
+A+ I+W TDYLL + S+ + I+ +VG + K E+PEDM+ R
Sbjct: 137 NAKIAIRWATDYLL----KATSQPDTIYVQVGDANKDHSCWERPEDMDTVR 183
>At2g32990 putative glucanse
Length = 525
Score = 32.0 bits (71), Expect = 0.55
Identities = 22/61 (36%), Positives = 27/61 (44%), Gaps = 7/61 (11%)
Query: 53 HARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGS---EKPEDMEYPRPTTTI*SEG 109
HA E IKWGTDY + S N + A+VG ++PEDM R I
Sbjct: 126 HALEAIKWGTDYFIKAHTSP----NVLWAEVGDGDTDHYCWQRPEDMTTSRRAFKIDENN 181
Query: 110 P 110
P
Sbjct: 182 P 182
>At4g11050 glucanase like protein
Length = 626
Score = 30.4 bits (67), Expect = 1.6
Identities = 22/66 (33%), Positives = 31/66 (46%), Gaps = 9/66 (13%)
Query: 53 HARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGS---EKPEDMEYPRPTTTI*SEG 109
HA + +KWGTDY F + E N ++ +VG ++PE+M R I
Sbjct: 113 HAIDAVKWGTDY----FIKAHPEPNVLYGEVGDGKSDHYCWQRPEEMTTDRRAYKIDRNN 168
Query: 110 P--DLA 113
P DLA
Sbjct: 169 PGSDLA 174
>At2g44570 putative glucanase
Length = 492
Score = 30.4 bits (67), Expect = 1.6
Identities = 21/61 (34%), Positives = 32/61 (52%), Gaps = 9/61 (14%)
Query: 58 IKWGTDYLLLTFDSSASEINQIHAKVGGSLKGSE---KPEDMEYPRPTTTI*SE--GPDL 112
++W TDYL+ + E N ++ +VG + +PEDM PRP+ I + G DL
Sbjct: 125 LRWATDYLI----KAHPEPNVLYGQVGDANLDHACWMRPEDMTTPRPSYRIDPQHPGADL 180
Query: 113 A 113
A
Sbjct: 181 A 181
>At4g39010 putative endo-1,4-beta-glucanase
Length = 497
Score = 30.0 bits (66), Expect = 2.1
Identities = 18/57 (31%), Positives = 31/57 (53%), Gaps = 8/57 (14%)
Query: 58 IKWGTDYLLLTFDSSASEI-NQIHAKVGGSLKGS---EKPEDMEYPRPTTTI*SEGP 110
++W ++YLL S S++ N+I +VG + E+PEDM+ PR + + P
Sbjct: 120 LRWSSNYLL----KSVSQLPNRIFVQVGDPIADHNCWERPEDMDTPRTAYAVNAPNP 172
>At4g38990 putative endo-1,4-beta-glucanase
Length = 494
Score = 30.0 bits (66), Expect = 2.1
Identities = 21/61 (34%), Positives = 31/61 (50%), Gaps = 8/61 (13%)
Query: 58 IKWGTDYLLLTFDSSASEINQIHAKVGGSLKGS---EKPEDMEYPRPTTTI*SEGP--DL 112
++WG++YLL T N+I +VG E+PEDM+ PR + + P DL
Sbjct: 114 LRWGSNYLLKTVSQLP---NRIFVQVGEPTPDHQCWERPEDMDTPRTAYALEAPKPASDL 170
Query: 113 A 113
A
Sbjct: 171 A 171
>At2g44550 putative glucanase
Length = 490
Score = 29.6 bits (65), Expect = 2.7
Identities = 22/66 (33%), Positives = 34/66 (51%), Gaps = 9/66 (13%)
Query: 53 HARELIKWGTDYLLLTFDSSASEINQIHAKVG---GSLKGSEKPEDMEYPRPTTTI*SE- 108
+A +KW TDYL+ + + N ++ +VG K +PEDM PR + I ++
Sbjct: 120 NALRALKWATDYLI----KAHPQPNVLYGQVGEGNSDHKCWMRPEDMTTPRTSYRIDAQH 175
Query: 109 -GPDLA 113
G DLA
Sbjct: 176 PGSDLA 181
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.343 0.153 0.541
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,946,452
Number of Sequences: 26719
Number of extensions: 327161
Number of successful extensions: 1269
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 1237
Number of HSP's gapped (non-prelim): 34
length of query: 367
length of database: 11,318,596
effective HSP length: 101
effective length of query: 266
effective length of database: 8,619,977
effective search space: 2292913882
effective search space used: 2292913882
T: 11
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.5 bits)
S2: 61 (28.1 bits)
Medicago: description of AC127169.10