BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126786.7 - phase: 0 /pseudo
(521 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g17480 membrane protein Mlo8 148 8e-36
At1g42560 membrane protein Mlo9 140 2e-33
At2g33670 similar to Mlo proteins from H. vulgare 135 5e-32
At5g65970 Mlo protein-like 119 3e-27
At1g61560 unknown protein 111 8e-25
At1g11310 Mlo like protein 106 4e-23
At4g02600 AtMlo-h1-like protein 103 3e-22
At3g45290 membrane protein Mlo3 (Mlo3) 101 1e-21
At2g17430 membrane protein Mlo7 97 2e-20
At4g24250 membrane protein Mlo13 94 1e-19
At2g39200 membrane protein Mlo12 94 1e-19
At2g44110 similar to Mlo proteins from H. vulgare 93 3e-19
At1g26700 membrane protein Mlo14 84 2e-16
At5g53760 unknown protein 82 6e-16
At1g11000 membrane protein Mlo4 70 2e-12
At5g24950 cytochrome P-450-like protein 37 0.027
At5g24960 cytochrome P450-like protein 33 0.29
At1g78110 unknown protein 32 1.1
At2g30770 putative cytochrome P450 30 2.5
At3g63500 putative protein 28 9.4
>At2g17480 membrane protein Mlo8
Length = 593
Score = 148 bits (373), Expect = 8e-36
Identities = 81/159 (50%), Positives = 96/159 (59%), Gaps = 25/159 (15%)
Query: 11 RQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEKIKAELMVLGF 70
+QL+ TPTWAVAAVC +++S+LLEKL+HK V +R K ALL+ALEKIKAELMVLGF
Sbjct: 39 KQLNQTPTWAVAAVCTFFIVVSVLLEKLLHKVGKVLWDRHKTALLDALEKIKAELMVLGF 98
Query: 71 ISLLLTFGQNYISKVCIPVKYSNTMLPCQPLAERTADHPGEPALEPQGTEHEPTPNIPAG 130
ISLLLTFGQ YI +CIP + TMLPC P PN+
Sbjct: 99 ISLLLTFGQTYILDICIPSHVARTMLPC------------------------PAPNLKK- 133
Query: 131 EGESKGEHHRRLLSYERRFLGGGGGGPGCKPWTGYISYI 169
E + GE HRRLLS+E RFL GG P GY+ I
Sbjct: 134 EDDDNGESHRRLLSFEHRFLSGGEASPTKCTKEGYVELI 172
>At1g42560 membrane protein Mlo9
Length = 460
Score = 140 bits (352), Expect = 2e-33
Identities = 67/100 (67%), Positives = 80/100 (80%), Gaps = 1/100 (1%)
Query: 2 GGGGGGGDS-RQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEK 60
GGGGGGG+ RQLD TPTWAV+ VC +I++ISI+LE +IHK VFE +KK AL EALEK
Sbjct: 4 GGGGGGGEGPRQLDQTPTWAVSTVCGVIILISIILELIIHKVGEVFERKKKKALFEALEK 63
Query: 61 IKAELMVLGFISLLLTFGQNYISKVCIPVKYSNTMLPCQP 100
IK ELMVLGFISLLLTFGQNYI+ +C+P +Y + M C P
Sbjct: 64 IKNELMVLGFISLLLTFGQNYIASICVPSRYGHAMSFCGP 103
>At2g33670 similar to Mlo proteins from H. vulgare
Length = 501
Score = 135 bits (340), Expect = 5e-32
Identities = 70/125 (56%), Positives = 87/125 (69%), Gaps = 9/125 (7%)
Query: 1 MGGGGGGGDS----RQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLE 56
M GGGGG S R+LD TPTWAV+ VC +I++ISI+LE +IHK VF ER+K AL E
Sbjct: 1 MAGGGGGSTSGEGPRELDQTPTWAVSTVCGVIILISIVLELMIHKIGEVFTERRKKALYE 60
Query: 57 ALEKIKAELMVLGFISLLLTFGQNYISKVCIPVKYSNTMLPCQPLAERTADHPGEPALEP 116
AL+KIK ELMVLGFISLLLTFGQNYI+ +C+ +Y + M C P D P + +P
Sbjct: 61 ALQKIKNELMVLGFISLLLTFGQNYIASLCVASRYGHAMSFCGPY-----DGPSGESKKP 115
Query: 117 QGTEH 121
+ TEH
Sbjct: 116 KTTEH 120
>At5g65970 Mlo protein-like
Length = 569
Score = 119 bits (299), Expect = 3e-27
Identities = 56/93 (60%), Positives = 72/93 (77%)
Query: 6 GGGDSRQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEKIKAEL 65
GG + L TPTWAVA VC +++S+LLEK +H+ A+ E+ K++LLEALEKIKAEL
Sbjct: 29 GGAKEKGLSQTPTWAVALVCTFFILVSVLLEKALHRVATWLWEKHKNSLLEALEKIKAEL 88
Query: 66 MVLGFISLLLTFGQNYISKVCIPVKYSNTMLPC 98
M+LGFISLLLTFG+ YI K+CIP K + +MLPC
Sbjct: 89 MILGFISLLLTFGEQYILKICIPEKAAASMLPC 121
>At1g61560 unknown protein
Length = 583
Score = 111 bits (278), Expect = 8e-25
Identities = 53/93 (56%), Positives = 70/93 (74%)
Query: 11 RQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEKIKAELMVLGF 70
+ L+ T TWAVA VC ++++ISI++EKLIHK S F+++ K AL EALEK+KAELM++GF
Sbjct: 8 KTLEETSTWAVAVVCFVLLLISIVIEKLIHKIGSWFKKKNKKALYEALEKVKAELMLMGF 67
Query: 71 ISLLLTFGQNYISKVCIPVKYSNTMLPCQPLAE 103
ISLLLT GQ YIS +CIP + +M PC E
Sbjct: 68 ISLLLTIGQGYISNICIPKNIAASMHPCSASEE 100
>At1g11310 Mlo like protein
Length = 573
Score = 106 bits (264), Expect = 4e-23
Identities = 53/93 (56%), Positives = 67/93 (71%)
Query: 11 RQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEKIKAELMVLGF 70
R L+ T TWAVA VC +++ ISI+LE IHK + F+++ K AL EALEK+KAELM+LGF
Sbjct: 8 RTLEETSTWAVAVVCFVLLFISIVLEHSIHKIGTWFKKKHKQALFEALEKVKAELMLLGF 67
Query: 71 ISLLLTFGQNYISKVCIPVKYSNTMLPCQPLAE 103
ISLLLT GQ IS +CI K ++TM PC E
Sbjct: 68 ISLLLTIGQTPISNICISQKVASTMHPCSAAEE 100
>At4g02600 AtMlo-h1-like protein
Length = 526
Score = 103 bits (256), Expect = 3e-22
Identities = 51/102 (50%), Positives = 68/102 (66%)
Query: 5 GGGGDSRQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEKIKAE 64
G GG+ L+ TPTW VA VC +IV IS+ +E+L+H F +V +++K+ L EAL+K+K E
Sbjct: 2 GHGGEGMSLEFTPTWVVAGVCTVIVAISLAVERLLHYFGTVLKKKKQKPLYEALQKVKEE 61
Query: 65 LMVLGFISLLLTFGQNYISKVCIPVKYSNTMLPCQPLAERTA 106
LM+LGFISLLLT Q ISK C+ MLPC + R A
Sbjct: 62 LMLLGFISLLLTVFQGLISKFCVKENVLMHMLPCSLDSRREA 103
>At3g45290 membrane protein Mlo3 (Mlo3)
Length = 508
Score = 101 bits (251), Expect = 1e-21
Identities = 46/92 (50%), Positives = 68/92 (73%)
Query: 8 GDSRQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEKIKAELMV 67
G R L TPTWA+A VC + +SI LE+LI+ ++ ++ +K +LLEA+EK+K+ LMV
Sbjct: 14 GAVRSLQETPTWALATVCFFFIAVSICLERLINLLSTRLKKNRKTSLLEAVEKLKSVLMV 73
Query: 68 LGFISLLLTFGQNYISKVCIPVKYSNTMLPCQ 99
LGF+SL+L + +SK+CIP+KY+N MLPC+
Sbjct: 74 LGFMSLMLNVTEGEVSKICIPIKYANRMLPCR 105
>At2g17430 membrane protein Mlo7
Length = 572
Score = 97.1 bits (240), Expect = 2e-20
Identities = 54/124 (43%), Positives = 72/124 (57%), Gaps = 30/124 (24%)
Query: 5 GGGGDSRQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASV------------------- 45
G ++L TPTWAVA VC +++IS LLEK + + A+V
Sbjct: 25 GAPSGGKELSQTPTWAVAVVCTFLILISHLLEKGLQRLANVCFLLLLLLFLRVFLFKHSL 84
Query: 46 -----------FEERKKHALLEALEKIKAELMVLGFISLLLTFGQNYISKVCIPVKYSNT 94
++ K++LLEALEKIKAELM+LGFISLLLTFG+ YI K+C+P K + +
Sbjct: 85 SETYEFVSSQWLWKKHKNSLLEALEKIKAELMILGFISLLLTFGEPYILKICVPRKAALS 144
Query: 95 MLPC 98
MLPC
Sbjct: 145 MLPC 148
>At4g24250 membrane protein Mlo13
Length = 478
Score = 94.4 bits (233), Expect = 1e-19
Identities = 47/98 (47%), Positives = 64/98 (64%), Gaps = 1/98 (1%)
Query: 10 SRQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEKIKAELMVLG 69
S L+ TPTW VA +C IIV++S+L E+ +H + R++ AL EAL+K+K ELM+LG
Sbjct: 6 SGSLEYTPTWVVAFICFIIVLLSLLAERGLHHLGKCLKRRQQDALFEALQKLKEELMLLG 65
Query: 70 FISLLLTFGQNYISKVCIPVKYSNTMLPC-QPLAERTA 106
FISL+LT Q I +C+P N M PC +PL E A
Sbjct: 66 FISLMLTVSQAAIRHICVPPALVNNMFPCKKPLEEHHA 103
>At2g39200 membrane protein Mlo12
Length = 576
Score = 94.4 bits (233), Expect = 1e-19
Identities = 46/88 (52%), Positives = 62/88 (70%)
Query: 11 RQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEKIKAELMVLGF 70
R L+ TPTWAVA VC +++ ISI++E +H F+++ K AL EALEK+KAELM+LGF
Sbjct: 6 RSLEETPTWAVAVVCFVLLFISIMIEYFLHFIGHWFKKKHKKALSEALEKVKAELMLLGF 65
Query: 71 ISLLLTFGQNYISKVCIPVKYSNTMLPC 98
ISLLL Q +S++CIP + T PC
Sbjct: 66 ISLLLVVLQTPVSEICIPRNIAATWHPC 93
>At2g44110 similar to Mlo proteins from H. vulgare
Length = 496
Score = 93.2 bits (230), Expect = 3e-19
Identities = 47/93 (50%), Positives = 64/93 (68%), Gaps = 2/93 (2%)
Query: 6 GGGDSRQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEKIKAEL 65
GGG + L+ TPTW VA VC++IV IS +E+LIH+ F+ + L AL+KIK EL
Sbjct: 3 GGGTT--LEYTPTWVVALVCSVIVSISFAVERLIHRAGKHFKNNDQKQLFGALQKIKEEL 60
Query: 66 MVLGFISLLLTFGQNYISKVCIPVKYSNTMLPC 98
M++GFISLLL+ GQ+ I+K+CI + S LPC
Sbjct: 61 MLVGFISLLLSVGQSKIAKICISKELSEKFLPC 93
>At1g26700 membrane protein Mlo14
Length = 554
Score = 83.6 bits (205), Expect = 2e-16
Identities = 40/91 (43%), Positives = 61/91 (66%), Gaps = 1/91 (1%)
Query: 11 RQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEKIKAELMVLGF 70
R L +TPTW+VA V I V +S+++E+ IH+ ++ ++ K+ L ALEK+K ELM+LGF
Sbjct: 10 RTLGLTPTWSVATVLTIFVFVSLIVERSIHRLSNWLQKTKRKPLFAALEKMKEELMLLGF 69
Query: 71 ISLLLTFGQNYISKVCIPVKYSN-TMLPCQP 100
ISLLLT + I+ +C+ + N +PC P
Sbjct: 70 ISLLLTATSSTIANICVSSSFHNDRFVPCTP 100
>At5g53760 unknown protein
Length = 573
Score = 82.4 bits (202), Expect = 6e-16
Identities = 38/97 (39%), Positives = 64/97 (65%), Gaps = 1/97 (1%)
Query: 3 GGGGGGDSRQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEKIK 62
G + R L ++PTW+VA V + V++S+++E+ I++ ++ + K+ + ALEK+K
Sbjct: 8 GNEADSNERSLALSPTWSVAIVLTVFVVVSLIVERSIYRLSTWLRKTKRKPMFAALEKMK 67
Query: 63 AELMVLGFISLLLTFGQNYISKVCIPVK-YSNTMLPC 98
ELM+LGFISLLLT + I+ +C+P Y++ LPC
Sbjct: 68 EELMLLGFISLLLTATSSTIANICVPSSFYNDRFLPC 104
>At1g11000 membrane protein Mlo4
Length = 573
Score = 70.5 bits (171), Expect = 2e-12
Identities = 32/79 (40%), Positives = 55/79 (69%)
Query: 9 DSRQLDVTPTWAVAAVCAIIVIISILLEKLIHKFASVFEERKKHALLEALEKIKAELMVL 68
+ R L TPT++VA+V ++V + L+E+ I++F ++ ++ AL +LEK+K ELM+L
Sbjct: 7 EGRSLAETPTYSVASVVTVLVFVCFLVERAIYRFGKWLKKTRRKALFTSLEKMKEELMLL 66
Query: 69 GFISLLLTFGQNYISKVCI 87
G ISLLL+ +IS++C+
Sbjct: 67 GLISLLLSQSARWISEICV 85
>At5g24950 cytochrome P-450-like protein
Length = 496
Score = 37.0 bits (84), Expect = 0.027
Identities = 19/66 (28%), Positives = 36/66 (53%), Gaps = 1/66 (1%)
Query: 28 IVIISILLEKLIHKFASVFEERKKHALLEALEKIKAELMVLGFISLLLTFGQNYISKVCI 87
+ ++ +L +K++ FA V EE + ++E +EK ++ L LL+T + S+V
Sbjct: 130 VCVVHLLNKKMVQSFAKVREEERS-VMMEKVEKASSDSSPLNLSKLLITLTSDVASRVSF 188
Query: 88 PVKYSN 93
K+SN
Sbjct: 189 GKKHSN 194
>At5g24960 cytochrome P450-like protein
Length = 497
Score = 33.5 bits (75), Expect = 0.29
Identities = 20/65 (30%), Positives = 34/65 (51%), Gaps = 1/65 (1%)
Query: 28 IVIISILLEKLIHKFASVFEERKKHALLEALEKIKAELMVLGFISLLLTFGQNYISKVCI 87
+ I+++L +K + F V EE ++E +EK ++ L LLLT + S+V +
Sbjct: 131 VCIVNLLNKKKVQSFEKVREEEISE-MMERVEKASSDSSPLNLSELLLTLTSDVTSRVSL 189
Query: 88 PVKYS 92
KYS
Sbjct: 190 GRKYS 194
>At1g78110 unknown protein
Length = 342
Score = 31.6 bits (70), Expect = 1.1
Identities = 15/35 (42%), Positives = 21/35 (59%), Gaps = 3/35 (8%)
Query: 124 TPNIPAGEGESKGEHHRRLLSYERRFLGGGGGGPG 158
+P+ P+ ++ HHRR LS + GGGGGG G
Sbjct: 39 SPSRPSDSSTNRRPHHRRQLS---KLSGGGGGGHG 70
>At2g30770 putative cytochrome P450
Length = 497
Score = 30.4 bits (67), Expect = 2.5
Identities = 17/65 (26%), Positives = 34/65 (52%), Gaps = 1/65 (1%)
Query: 28 IVIISILLEKLIHKFASVFEERKKHALLEALEKIKAELMVLGFISLLLTFGQNYISKVCI 87
+ I+++L K++ F V E+ + +A++E LEK + L +T + S+V +
Sbjct: 131 VCILNLLTNKMVESFEKVRED-EVNAMIEKLEKASSSSSSENLSELFITLPSDVTSRVAL 189
Query: 88 PVKYS 92
K+S
Sbjct: 190 GRKHS 194
>At3g63500 putative protein
Length = 1162
Score = 28.5 bits (62), Expect = 9.4
Identities = 9/16 (56%), Positives = 11/16 (68%)
Query: 409 SCSWGGCSICLQLYHT 424
+CSW GC +CL HT
Sbjct: 850 TCSWVGCDVCLHWCHT 865
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.354 0.158 0.568
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,388,472
Number of Sequences: 26719
Number of extensions: 410313
Number of successful extensions: 4399
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 4370
Number of HSP's gapped (non-prelim): 27
length of query: 521
length of database: 11,318,596
effective HSP length: 104
effective length of query: 417
effective length of database: 8,539,820
effective search space: 3561104940
effective search space used: 3561104940
T: 11
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC126786.7