BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126783.2 + phase: 0
(147 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g70600 60S ribosomal protein L27A 265 9e-72
At1g23290 60s ribosomal protein l27a. 258 1e-69
At1g12960 60S ribosomal protein L27a, putative 123 4e-29
At5g17650 glycine/proline-rich protein 36 0.008
At5g27450 mevalonate kinase 30 0.60
At1g30040 unknown protein 28 1.8
At3g58230 putative protein 27 3.0
At2g01840 putative non-LTR retroelement reverse transcriptase 27 3.0
At3g52840 beta-galactosidase precursor - like protein 27 3.9
At1g66260 RNA and export factor binding protein, putative 27 3.9
At3g63100 putative protein 27 5.1
At2g05510 putative glycine-rich protein 27 5.1
At5g63720 unknown protein 26 6.7
At5g57870 eukaryotic initiation factor 4, eIF4-like protein 26 6.7
At4g38010 putative protein 26 8.7
At3g30820 hypothetical protein 26 8.7
At2g40700 ATP-dependent RNA helicase 26 8.7
At2g06110 putative Athila retroelement ORF1 protein 26 8.7
At2g05540 putative glycine-rich protein 26 8.7
At2g05090 putative replication protein A1 26 8.7
>At1g70600 60S ribosomal protein L27A
Length = 146
Score = 265 bits (676), Expect = 9e-72
Identities = 126/147 (85%), Positives = 137/147 (92%), Gaps = 1/147 (0%)
Query: 1 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY
Sbjct: 1 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
Query: 61 FHKLRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQP 120
FHKLRNKF+ PIVN+DK+ SL+P+DVK K+ K+N P+IDVTQ GFFK+LGKG LP+N+P
Sbjct: 61 FHKLRNKFFCPIVNLDKLWSLVPEDVKAKSTKDN-VPLIDVTQHGFFKVLGKGHLPENKP 119
Query: 121 FVVKTKLISKIAEKKIKEAGGAVVLTA 147
FVVK KLISK AEKKIKEAGGAVVLTA
Sbjct: 120 FVVKAKLISKTAEKKIKEAGGAVVLTA 146
>At1g23290 60s ribosomal protein l27a.
Length = 146
Score = 258 bits (658), Expect = 1e-69
Identities = 123/147 (83%), Positives = 134/147 (90%), Gaps = 1/147 (0%)
Query: 1 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
M T KKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY
Sbjct: 1 MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
Query: 61 FHKLRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQP 120
FHKLRNKF+ PIVN+DK+ SL+P+DVK K+ K+N P+IDVTQ GFFK+LGKG LP+N+P
Sbjct: 61 FHKLRNKFFCPIVNLDKLWSLVPEDVKAKSSKDN-VPLIDVTQHGFFKVLGKGHLPENKP 119
Query: 121 FVVKTKLISKIAEKKIKEAGGAVVLTA 147
FVVK KLISK AEKKIKEAGGAVVLTA
Sbjct: 120 FVVKAKLISKTAEKKIKEAGGAVVLTA 146
>At1g12960 60S ribosomal protein L27a, putative
Length = 104
Score = 123 bits (308), Expect = 4e-29
Identities = 73/146 (50%), Positives = 80/146 (54%), Gaps = 46/146 (31%)
Query: 1 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
MTT KK R R HVS GHGR GKHRK PG RGNAG VGMRY
Sbjct: 1 MTTSRKKTRNLREHVSVGHGRFGKHRKLPGSRGNAG-------------------VGMRY 41
Query: 61 FHKLRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQP 120
FHKLRNKFY IVN+DK+ S++ LGKG LP+N+P
Sbjct: 42 FHKLRNKFYCQIVNLDKLWSMV---------------------------LGKGFLPENKP 74
Query: 121 FVVKTKLISKIAEKKIKEAGGAVVLT 146
VVK KL+S EKKIKEAG AVVLT
Sbjct: 75 VVVKAKLVSNTDEKKIKEAGSAVVLT 100
>At5g17650 glycine/proline-rich protein
Length = 173
Score = 35.8 bits (81), Expect = 0.008
Identities = 22/56 (39%), Positives = 27/56 (47%), Gaps = 5/56 (8%)
Query: 14 HVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRNKFY 69
H+S HG G H H G G G H + K+ G FGK GM F K + KF+
Sbjct: 119 HMSHHHGHYGHHHGHGYGYGYHG---HGKFKHGKFKHGKFGKHGM--FGKHKGKFF 169
>At5g27450 mevalonate kinase
Length = 378
Score = 29.6 bits (65), Expect = 0.60
Identities = 20/78 (25%), Positives = 35/78 (44%)
Query: 69 YNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQPFVVKTKLI 128
+N + +I K + I Q E ++ E + + ++ + LL GV + V+ T +
Sbjct: 265 FNAVDSISKELAAIIQSKDETSVTEKEERIKELMEMNQGLLLSMGVSHSSIEAVILTTVK 324
Query: 129 SKIAEKKIKEAGGAVVLT 146
K+ K GG VLT
Sbjct: 325 HKLVSKLTGAGGGGCVLT 342
>At1g30040 unknown protein
Length = 341
Score = 28.1 bits (61), Expect = 1.8
Identities = 17/58 (29%), Positives = 26/58 (44%), Gaps = 13/58 (22%)
Query: 73 VNIDKIASLIPQDVKEKALKENKAPVIDVT-------------QFGFFKLLGKGVLPQ 117
V +D SLIP L + PV+++ +FGFFK++ GV P+
Sbjct: 8 VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPE 65
>At3g58230 putative protein
Length = 153
Score = 27.3 bits (59), Expect = 3.0
Identities = 9/43 (20%), Positives = 27/43 (61%)
Query: 64 LRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGF 106
++N + N ++++ I L PQ++ E+ ++ + ++++ + GF
Sbjct: 44 VKNAYMNNLLDLIDIICLAPQELTEEEIRNAENTLLELVEVGF 86
>At2g01840 putative non-LTR retroelement reverse transcriptase
Length = 1715
Score = 27.3 bits (59), Expect = 3.0
Identities = 15/56 (26%), Positives = 29/56 (51%), Gaps = 11/56 (19%)
Query: 64 LRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQ 119
+R N + ID+ +PQ + ++A+ +N+ F LLG+ ++P+NQ
Sbjct: 5 VRRALQNINLGIDETPVALPQAIVQRAVDDNR-----------FCLLGRPLMPRNQ 49
>At3g52840 beta-galactosidase precursor - like protein
Length = 727
Score = 26.9 bits (58), Expect = 3.9
Identities = 16/59 (27%), Positives = 26/59 (43%)
Query: 8 NRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRN 66
N +G V IG+H RGN G ++ I +K + G+ R++H R+
Sbjct: 639 NTMGKGQVWVNGHNIGRHWPAYTARGNCGRCNYAGIYNEKKCLSHCGEPSQRWYHVPRS 697
>At1g66260 RNA and export factor binding protein, putative
Length = 295
Score = 26.9 bits (58), Expect = 3.9
Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Query: 4 RFKKNRKKRGHVSAGHGRI-GKHRKHPGGRGNAGG 37
R + ++G V+AG G G+ R + GGRGN G
Sbjct: 231 RLPLQQNQQGGVTAGRGGFRGRGRGNGGGRGNKSG 265
>At3g63100 putative protein
Length = 199
Score = 26.6 bits (57), Expect = 5.1
Identities = 15/36 (41%), Positives = 18/36 (49%), Gaps = 5/36 (13%)
Query: 12 RGHVSA---GHGRIGKHRKHPGGRGN--AGGMHHHR 42
RGH GH R G+ +H RG+ G HHHR
Sbjct: 96 RGHGHGHGHGHRRHGRDHRHGRDRGHHRGHGHHHHR 131
>At2g05510 putative glycine-rich protein
Length = 127
Score = 26.6 bits (57), Expect = 5.1
Identities = 12/28 (42%), Positives = 12/28 (42%)
Query: 13 GHVSAGHGRIGKHRKHPGGRGNAGGMHH 40
GH G G G H GG GG HH
Sbjct: 83 GHYGGGGGHYGGGGGHGGGGHYGGGGHH 110
>At5g63720 unknown protein
Length = 492
Score = 26.2 bits (56), Expect = 6.7
Identities = 17/64 (26%), Positives = 25/64 (38%), Gaps = 16/64 (25%)
Query: 4 RFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHK 63
RFK+ + K G + H H HHH DK P + K+ ++ HK
Sbjct: 356 RFKRIKNKIGQI------FHHHHHH----------HHHHHHHDKEKPSAWNKLQSKFHHK 399
Query: 64 LRNK 67
+ K
Sbjct: 400 HQEK 403
>At5g57870 eukaryotic initiation factor 4, eIF4-like protein
Length = 780
Score = 26.2 bits (56), Expect = 6.7
Identities = 13/28 (46%), Positives = 18/28 (63%), Gaps = 1/28 (3%)
Query: 11 KRGHVSAGHGRIGKHRKHPGGRGNAGGM 38
+RG VS+G G + +PGGR AGG+
Sbjct: 476 RRGMVSSG-GPVSPGPVYPGGRPGAGGL 502
>At4g38010 putative protein
Length = 559
Score = 25.8 bits (55), Expect = 8.7
Identities = 9/26 (34%), Positives = 16/26 (60%)
Query: 57 GMRYFHKLRNKFYNPIVNIDKIASLI 82
G RYFHK++++ YN ++ +I
Sbjct: 426 GRRYFHKMKSREYNLFPKLEHYGCMI 451
>At3g30820 hypothetical protein
Length = 405
Score = 25.8 bits (55), Expect = 8.7
Identities = 11/27 (40%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Query: 16 SAGHGRIGKHRKHPGGRGNAGGMHHHR 42
S GH R+G+ R+ PG + ++ HHR
Sbjct: 380 SLGH-RLGRSRRQPGEQSSSESPTHHR 405
>At2g40700 ATP-dependent RNA helicase
Length = 609
Score = 25.8 bits (55), Expect = 8.7
Identities = 15/46 (32%), Positives = 23/46 (49%)
Query: 79 ASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQPFVVK 124
A L Q ++ LKE K +T++ K+L K +P N P + K
Sbjct: 449 ALLFLQPIEIDYLKELKKHGASLTEYPLMKVLDKFPIPGNMPRIKK 494
>At2g06110 putative Athila retroelement ORF1 protein
Length = 616
Score = 25.8 bits (55), Expect = 8.7
Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 2/33 (6%)
Query: 50 PGYFGKVGMRYFHKLRNKFYNPIVNIDKIASLI 82
PGYF VG FH L + P+ +I++ L+
Sbjct: 126 PGYFALVGQHPFHGL--SYEQPMDHIERFEDLV 156
>At2g05540 putative glycine-rich protein
Length = 135
Score = 25.8 bits (55), Expect = 8.7
Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 5/47 (10%)
Query: 13 GHVSAGHG-RIGKHRKHPGGRGNAGGMHHHR----ILFDKYHPGYFG 54
G+ G+G R G +R GG GN GG + +R + + GY+G
Sbjct: 65 GNPGGGYGNRGGGYRNRDGGYGNRGGGYGNRGGGYCRYGCCYRGYYG 111
>At2g05090 putative replication protein A1
Length = 521
Score = 25.8 bits (55), Expect = 8.7
Identities = 12/30 (40%), Positives = 18/30 (60%)
Query: 109 LLGKGVLPQNQPFVVKTKLISKIAEKKIKE 138
LL GV+ + P KTK ++K+ K+ KE
Sbjct: 492 LLTAGVVDEIDPLASKTKGLTKVKTKRYKE 521
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.142 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,652,274
Number of Sequences: 26719
Number of extensions: 164798
Number of successful extensions: 427
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 401
Number of HSP's gapped (non-prelim): 30
length of query: 147
length of database: 11,318,596
effective HSP length: 90
effective length of query: 57
effective length of database: 8,913,886
effective search space: 508091502
effective search space used: 508091502
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC126783.2