BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126782.6 - phase: 1
(422 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g24830 argininosuccinate synthase like protein 703 0.0
At3g19060 hypothetical protein 32 0.66
At5g64300 GTP cyclohydrolase II; 3,4-dihydroxy-2-butanone-4-phos... 31 1.1
At5g23850 putative protein 31 1.1
At5g12950 unknown protein 30 1.9
At5g27330 glutamic acid-rich protein 30 2.5
At1g07620 GTP-binding protein like 30 2.5
At5g33300 putative protein 30 3.3
At1g10290 putative phragmoplastin 30 3.3
At2g15200 putative protein on transposon FARE2.7 (cds2) 29 4.3
At1g68990 DNA-directed RNA polymerase 29 4.3
At1g01950 unknown protein 29 4.3
At5g12960 unknown protein 28 9.5
At2g37390 unknown protein 28 9.5
At1g71830 protein kinase, putative 28 9.5
At1g34210 putative protein 28 9.5
>At4g24830 argininosuccinate synthase like protein
Length = 494
Score = 703 bits (1815), Expect = 0.0
Identities = 335/421 (79%), Positives = 391/421 (92%), Gaps = 1/421 (0%)
Query: 1 IKAVLYKD-VEISETKKVAGLKGKLNKVVLAYSGGLDTSVVVPWLRENYGCDVVCFTADL 59
I+AVL D ++ K AGL+GKL KVVLAYSGGLDTSV+VPWL+ENYGC+VVCFTAD+
Sbjct: 72 IRAVLSGDGTALTTDSKEAGLRGKLKKVVLAYSGGLDTSVIVPWLKENYGCEVVCFTADV 131
Query: 60 GQGASELEGLEEKAKASGASQLVVKDLKEEFVRDYVFPCLRAGAIYERKYLLGTAIARPV 119
GQG ELEGLE+KAKASGASQLVVKDL EEFV+D++FPCLRAGAIYERKYLLGT++ARPV
Sbjct: 132 GQGIKELEGLEQKAKASGASQLVVKDLTEEFVKDFIFPCLRAGAIYERKYLLGTSMARPV 191
Query: 120 IAKAMVDIANEVGADAVSHGCTGKGNDQVRFELSFFALNPKLNVVAPWREWDITGREDAI 179
IAKAMVD+A EVGADAV+HGCTGKGNDQVRFEL+FF+LNP+L VVAPWREW+I GREDAI
Sbjct: 192 IAKAMVDVAAEVGADAVAHGCTGKGNDQVRFELTFFSLNPELKVVAPWREWEIQGREDAI 251
Query: 180 EYAKKHDVPVPVTKKSIYSRDRNLWHITHEGDVLEDPANEPKKDMYMLTVDPEDAPNEPE 239
EYAKKH+VPVPVTKKSIYSRDRNLWH++HEGD+LEDPANEPKKDMYM++VDPEDAP++PE
Sbjct: 252 EYAKKHNVPVPVTKKSIYSRDRNLWHLSHEGDLLEDPANEPKKDMYMMSVDPEDAPDQPE 311
Query: 240 YVEIGFELGIPVSLNGKRLSPGNLVAELNEIGGRHGIGRVDLVEDRIVGMKSRGVYETPG 299
Y+EIG E G+PV+LNGK LSP L+AELN IGG+HGIGR+D+VE+R+VGMKSRGVYETPG
Sbjct: 312 YIEIGIESGLPVALNGKALSPATLLAELNTIGGKHGIGRIDMVENRLVGMKSRGVYETPG 371
Query: 300 GTILFTAARDLETLTLDRETLQLKDSLALKYAELVYAGRWFDPLRESMDSFMEKISQTTT 359
GTILF A ++LE+LTLDRE++Q+KD+LALKYAE+VYAGRWFDPLRESMD+FMEKI++TTT
Sbjct: 372 GTILFAAVQELESLTLDRESIQVKDTLALKYAEMVYAGRWFDPLRESMDAFMEKITETTT 431
Query: 360 GSVSLKLYKGSASVTGRKSPYSLYREDISSFESGEIYNHADAVGFIKLYGLPMRIRAMME 419
GSV+LKLYKGS SVTGR+SP SLYR+DISSFE EIYN ADA GFI+LYGLPM+IRAM++
Sbjct: 432 GSVTLKLYKGSVSVTGRQSPNSLYRQDISSFEGSEIYNQADAAGFIRLYGLPMKIRAMLK 491
Query: 420 Q 420
+
Sbjct: 492 K 492
>At3g19060 hypothetical protein
Length = 1647
Score = 32.0 bits (71), Expect = 0.66
Identities = 27/118 (22%), Positives = 53/118 (44%), Gaps = 3/118 (2%)
Query: 279 VDLVEDRI--VGMKSRGVYETPGGTILFTAARDLETLTLDRETLQLKDSLALKYAELVYA 336
+++++D++ + + + VY G T AA + + + L +E ++L+ + Y EL+
Sbjct: 80 IEVLKDQLLLISKQQKNVYSELGETKSAVAALESQNIILIQEAVELR-RIKENYFELLKK 138
Query: 337 GRWFDPLRESMDSFMEKISQTTTGSVSLKLYKGSASVTGRKSPYSLYREDISSFESGE 394
P +S K + + K K AS+ K LY+ DI+S G+
Sbjct: 139 QELDIPAMKSKQCDEFKDNPAEDSEIDTKFKKMQASLEKAKRLNMLYKSDIASKACGD 196
>At5g64300 GTP cyclohydrolase II;
3,4-dihydroxy-2-butanone-4-phoshate synthase
(emb|CAA03884.1)
Length = 543
Score = 31.2 bits (69), Expect = 1.1
Identities = 33/132 (25%), Positives = 46/132 (34%), Gaps = 13/132 (9%)
Query: 55 FTADLGQGASELEGLEEKAKASGASQLVVKDLKEEFVRDYVFPCLRAGAIYERKYLLGTA 114
FT + G+ + +A+ L +D K E R G I+ KY G
Sbjct: 212 FTVTVDAKHGTTTGVSARDRATTILSLASRDSKPEDFN-------RPGHIFPLKYREGGV 264
Query: 115 IARPVIAKAMVDIANEVGADAVSHGCTGKGNDQVRFELSFFALNPKLNVVAPWREWDITG 174
+ R +A VD+ G D V C +D A PKL A +
Sbjct: 265 LKRAGHTEASVDLTVLAGLDPVGVLCEIVDDD------GSMARLPKLREFAAENNLKVVS 318
Query: 175 REDAIEYAKKHD 186
D I Y +K D
Sbjct: 319 IADLIRYRRKRD 330
>At5g23850 putative protein
Length = 542
Score = 31.2 bits (69), Expect = 1.1
Identities = 30/104 (28%), Positives = 49/104 (46%), Gaps = 18/104 (17%)
Query: 44 LRENYGCDVVCFTADLGQGASELEGLEEKAKASGASQLVVKDLKEEFVRDYVFPCLRAGA 103
+RE+ C + F D G S ++ ++ KA AS + +DLK ++V DY++ L
Sbjct: 399 VREHDKCRSIKFAVDWGN--SHIQKAQDIGKA--ASDFIQQDLKMDYVYDYMYHLLT--- 451
Query: 104 IYERKYLLGTAIARPVIAKAMVDIANEVGADAVSHGCTGKGNDQ 147
E LL +P I + V+I +E A C GN++
Sbjct: 452 --EYSKLLQ---FKPEIPRNAVEICSETMA------CLRSGNER 484
>At5g12950 unknown protein
Length = 861
Score = 30.4 bits (67), Expect = 1.9
Identities = 14/43 (32%), Positives = 26/43 (59%), Gaps = 1/43 (2%)
Query: 337 GRWFDPLRESMDSFMEKISQTTTGSVSLKLYKGSASVTGRKSP 379
G+W P+ E+ +S++ +SQ +G+VS + ++T R SP
Sbjct: 651 GKWITPIPETQNSYLVTLSQ-QSGNVSYVFSNSNQTITMRVSP 692
>At5g27330 glutamic acid-rich protein
Length = 628
Score = 30.0 bits (66), Expect = 2.5
Identities = 44/196 (22%), Positives = 80/196 (40%), Gaps = 26/196 (13%)
Query: 175 REDAIEYAKK----HDVPVPVTKK------SIYSRDRNLWHITHEGDVLEDPANEPKKDM 224
RE +E KK +++ +TK+ + +++L +T E + NE K+
Sbjct: 276 REMIVELEKKLGDMNEIVESLTKEREGLRGQVVGLEKSLDEVTEEAKARAEQINELVKEK 335
Query: 225 YMLTVDPEDAPNEPEYVEIGFELGIPVSLNGKRLSPGNLVAELNEIGGRHGIGRVDLVE- 283
+ + E E ++ E+ + V + K L+ E NE+ R ++VE
Sbjct: 336 TVKESELEGLMVENNSIKKEIEMAM-VQFSDKEKLVEQLLREKNELVQRVVNQEAEIVEL 394
Query: 284 DRIVGMKSRGVYETPGGTILFTAARDLETLTLDRETL-----QLKDSLALKYAELVYAGR 338
++ G + V + +D + E L QLKD+LAL E AG+
Sbjct: 395 SKLAGEQKHAVAQL---------RKDYNDQIKNGEKLNCNVSQLKDALALVEVERDNAGK 445
Query: 339 WFDPLRESMDSFMEKI 354
D + +M + EK+
Sbjct: 446 ALDEEKRNMVALKEKV 461
>At1g07620 GTP-binding protein like
Length = 1016
Score = 30.0 bits (66), Expect = 2.5
Identities = 18/70 (25%), Positives = 38/70 (53%), Gaps = 5/70 (7%)
Query: 181 YAKKHDVPVPVTKKSIYSRDRNLWHITHEGDVLEDPANEPKKDMYMLTVDPEDAPNEPEY 240
+ ++ ++P V +S +D + W + G ++EDPA+E D++ T+ D ++ E
Sbjct: 665 HLQEGEIPSQVENES--PKDLDPWDLP--GSLVEDPASEENSDVHQETMSESD-QDDTEQ 719
Query: 241 VEIGFELGIP 250
+ +LG+P
Sbjct: 720 ESLTRQLGMP 729
>At5g33300 putative protein
Length = 439
Score = 29.6 bits (65), Expect = 3.3
Identities = 32/144 (22%), Positives = 57/144 (39%), Gaps = 35/144 (24%)
Query: 10 EISETKKVAGLKGKLNKVVLAYSGGLDTSVVVPWLRENYGCDVVCFTADLGQGASELEGL 69
EI E+ +GL KLNK+ Y + + ++ F+ + G +E EG
Sbjct: 201 EIQESSNESGLLMKLNKIHSDYERQM----------KEMAEEIKRFSLEAGLLKAEFEGE 250
Query: 70 EEKAKASGASQL-------VVKDLKEEFVRDYVFPCLRAGAIYERKYLLGTAIARPVIAK 122
+ AS +Q+ +K+LKEEF + L T +++ + K
Sbjct: 251 QSSCSASCDNQINHTPMDSELKELKEEFNK------------------LSTLVSQMEMTK 292
Query: 123 AMVDIANEVGADAVSHGCTGKGND 146
+ ++V ++ V T K D
Sbjct: 293 SQFSETDKVQSEPVERSITSKNID 316
>At1g10290 putative phragmoplastin
Length = 914
Score = 29.6 bits (65), Expect = 3.3
Identities = 22/74 (29%), Positives = 32/74 (42%), Gaps = 4/74 (5%)
Query: 134 DAVSHGCTGKGNDQVRFELSFFALNPKLNVVAPWREWDITGREDAIEYAKKHD----VPV 189
D +S G +GK D++ +L P V P + I EYA+ +D V V
Sbjct: 113 DRLSKGASGKNRDEINLKLRTSTAPPLKLVDLPGLDQRIVDESMIAEYAQHNDAILLVIV 172
Query: 190 PVTKKSIYSRDRNL 203
P ++ S S R L
Sbjct: 173 PASQASEISSSRAL 186
>At2g15200 putative protein on transposon FARE2.7 (cds2)
Length = 650
Score = 29.3 bits (64), Expect = 4.3
Identities = 15/34 (44%), Positives = 20/34 (58%)
Query: 61 QGASELEGLEEKAKASGASQLVVKDLKEEFVRDY 94
QG E+EG EEK + G + K LKEE V ++
Sbjct: 503 QGDEEMEGEEEKQEEEGKEEEEEKVLKEENVEEH 536
>At1g68990 DNA-directed RNA polymerase
Length = 976
Score = 29.3 bits (64), Expect = 4.3
Identities = 29/126 (23%), Positives = 54/126 (42%), Gaps = 29/126 (23%)
Query: 306 AARDLETLTLDRETLQLKDSLALKYAELVYAGRWFDPLRESMDSFMEKISQTTTGSVSLK 365
AAR+ + D +L +L Y + ++ G WF+P+R ++ ++ + K
Sbjct: 150 AARECREILADMCEQKLAPNLP--YMKSLFLG-WFEPVRNAIQDDLD----------TFK 196
Query: 366 LYKGSASVTGRKSPYSLYREDISSFESGEIYNH---------ADAVGFIKLYGLPMRIRA 416
+ KG K PY+ + E + + + I H A+ VG +KL +I
Sbjct: 197 IKKG-------KIPYAPFMEQLPADKMAVITMHKMMGLLMTNAEGVGIVKLVNAATQIGE 249
Query: 417 MMEQGI 422
+EQ +
Sbjct: 250 AVEQEV 255
>At1g01950 unknown protein
Length = 893
Score = 29.3 bits (64), Expect = 4.3
Identities = 26/108 (24%), Positives = 50/108 (46%), Gaps = 4/108 (3%)
Query: 283 EDRIVGMKSRGVYETPGGTILFTAARDLETLTLDRETLQ---LKDSLALKYAELVYAGRW 339
E+ + +KS+ +T G L+ L L+ E LQ L++ + + ++LV
Sbjct: 526 EEEVSKVKSQSTLKTRSGEGEDAGITRLQKL-LEDEALQKKKLEEEVTILRSQLVQLTFE 584
Query: 340 FDPLRESMDSFMEKISQTTTGSVSLKLYKGSASVTGRKSPYSLYREDI 387
D +R +D S + T S+ + + SV G+K+P++ E +
Sbjct: 585 ADQMRRCLDRGAPGNSYSGTDSLPSRHSQARESVNGQKAPFATLCEQV 632
>At5g12960 unknown protein
Length = 865
Score = 28.1 bits (61), Expect = 9.5
Identities = 13/44 (29%), Positives = 26/44 (58%), Gaps = 1/44 (2%)
Query: 336 AGRWFDPLRESMDSFMEKISQTTTGSVSLKLYKGSASVTGRKSP 379
AG W P+ E+++S + +SQ +G++S L + ++ + SP
Sbjct: 654 AGNWITPIPETLNSHLVTLSQ-QSGNISYVLSNSNQTIIMKVSP 696
>At2g37390 unknown protein
Length = 259
Score = 28.1 bits (61), Expect = 9.5
Identities = 15/41 (36%), Positives = 23/41 (55%), Gaps = 3/41 (7%)
Query: 341 DPLRESMDSFMEKISQTTTGSVS---LKLYKGSASVTGRKS 378
D R D F++ + G +S L++YKG S+TGR+S
Sbjct: 48 DGRRSFADDFIKLPTSAEDGEMSNKKLEIYKGRRSITGRRS 88
>At1g71830 protein kinase, putative
Length = 625
Score = 28.1 bits (61), Expect = 9.5
Identities = 16/55 (29%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
Query: 272 GRHGIGRV---DLVEDRIVGMKSRGVYETPGGTILFTAARDLETLTLDRETLQLK 323
GR G G+V L + +V +K TPGG + F ++ ++ + R L+L+
Sbjct: 309 GRGGFGKVYKGRLADGTLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLR 363
>At1g34210 putative protein
Length = 628
Score = 28.1 bits (61), Expect = 9.5
Identities = 16/55 (29%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
Query: 272 GRHGIGRV---DLVEDRIVGMKSRGVYETPGGTILFTAARDLETLTLDRETLQLK 323
GR G G+V L + +V +K TPGG + F ++ ++ + R L+L+
Sbjct: 312 GRGGFGKVYKGRLADGTLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLR 366
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.137 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,273,776
Number of Sequences: 26719
Number of extensions: 410732
Number of successful extensions: 1023
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 1019
Number of HSP's gapped (non-prelim): 18
length of query: 422
length of database: 11,318,596
effective HSP length: 102
effective length of query: 320
effective length of database: 8,593,258
effective search space: 2749842560
effective search space used: 2749842560
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC126782.6