BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125478.7 - phase: 0
(357 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17930 unknown protein 97 1e-20
At2g25010 unknown protein 82 6e-16
At1g48120 serine/threonine phosphatase PP7, putative 69 5e-12
At1g50760 hypothetical protein 32 0.53
At3g53180 nodulin / glutamate-ammonia ligase - like protein 30 2.6
At1g10680 putative P-glycoprotein-2 emb|CAA71277 29 4.5
At4g08710 putative protein 28 7.7
At1g50750 hypothetical protein 28 7.7
>At1g17930 unknown protein
Length = 478
Score = 97.1 bits (240), Expect = 1e-20
Identities = 76/285 (26%), Positives = 131/285 (45%), Gaps = 35/285 (12%)
Query: 2 PMGEMTVTLDDVACLTHLSIEGRMLAHGKKMPKHEGAALLMTYLGVAQHEAEKICNQEY- 60
P GEMT+TLD+V+ + L+++G+ + G K + + + + LG K+ E
Sbjct: 84 PCGEMTITLDEVSLILGLAVDGKPVV-GVKEKDEDPSQVCLRLLG-------KLPKGELS 135
Query: 61 GGYISYPRLRDFYTSYLGRANVLAGTEDPEEVEELERVRTYCVRCYLLYLVGCLLFGDRS 120
G ++ L++ + A + +E+E Y R YL+Y+VG +F
Sbjct: 136 GNRVTAKWLKESFAECPKGATM-------KEIE-------YHTRAYLIYIVGSTIFATTD 181
Query: 121 NKRIELIYLTTMADGYAGMRNYSWGAMTLAYLYGELADACRLGHRALGGSVTLLTAWFLA 180
+I + YL D + Y+WGA LA+LY ++ +A + +GG +TLL W
Sbjct: 182 PSKISVDYLILFED-FEKAGEYAWGAAALAFLYRQIGNASQRSQSIIGGCLTLLQCWSYF 240
Query: 181 HFPGFFSVDLNTDYLENYSVAARWK---LQKGHGEGITYRSLLDRIQLDDVCWRSYEEHR 237
H ++D + +A WK + + YR LD + +V W +E
Sbjct: 241 H----LNIDRPKRTTRQFPLALLWKGRQQSRSKNDLFKYRKALDDLDPSNVSWCPFEGDL 296
Query: 238 EI--QDFEE--VFWYSRWIMCGVRRVYRHLPERVLRQYGYVQTIP 278
+I Q F++ + SR + G + V H P+R ++Q+G Q IP
Sbjct: 297 DIVPQSFKDNLLLGRSRTKLIGPKVVEWHFPDRCMKQFGLCQVIP 341
>At2g25010 unknown protein
Length = 509
Score = 81.6 bits (200), Expect = 6e-16
Identities = 75/286 (26%), Positives = 125/286 (43%), Gaps = 33/286 (11%)
Query: 1 MPMGEMTVTLDDVACLTHLSIEGRMLAHGKKMPKHEGAALLMTYLGVAQHEAEKICNQEY 60
+P+GEMT+TLD+VA + L I+G + K G + M G + N+E
Sbjct: 92 LPLGEMTITLDEVALVLGLEIDGDPIVGSK-----VGDEVAMDMCGRLLGKLPSAANKE- 145
Query: 61 GGYISYPRLRDFYTSYLGRANVLAGTEDPEEVEELERVRTYCVRCYLLYLVGCLLFGDRS 120
++ R++ ++L R +E PE+ + V+ + R YLLYL+G +F
Sbjct: 146 ---VNCSRVK---LNWLKR----TFSECPEDA-SFDVVKCH-TRAYLLYLIGSTIFATTD 193
Query: 121 NKRIELIYLTTMADGYAGMRNYSWGAMTLAYLYGELADACRLGHRALGGSVTLLTAWFLA 180
++ + YL D + Y+WGA LA LY L +A + G +TLL W
Sbjct: 194 GDKVSVKYLPLFED-FDQAGRYAWGAAALACLYRALGNASLKSQSNICGCLTLLQCW--- 249
Query: 181 HFPGFFSVDLNTDYLEN--YSVAARWKLQ--KGHGEGITYRSLLDRIQLDDVCWRSYEEH 236
+F +D+ + +A WK + + + YR LD + + W YE
Sbjct: 250 ---SYFHLDIGRPEKSEACFPLALLWKGKGSRSKTDLSEYRRELDDLDPSKITWCPYERF 306
Query: 237 REI----QDFEEVFWYSRWIMCGVRRVYRHLPERVLRQYGYVQTIP 278
+ + + S+ + ++ H P+R LRQ+G Q IP
Sbjct: 307 ENLIPPHIKAKLILGRSKTTLVCFEKIELHFPDRCLRQFGKRQPIP 352
>At1g48120 serine/threonine phosphatase PP7, putative
Length = 1338
Score = 68.6 bits (166), Expect = 5e-12
Identities = 80/297 (26%), Positives = 122/297 (40%), Gaps = 44/297 (14%)
Query: 1 MPMGEMTVTLDDVACLTHLSIEGRMLAHGKKMPKHEGAALLMTYLGVAQHEAEKICNQEY 60
+P GE+TVTL DV L L ++G + K + A L LG + +
Sbjct: 101 LPAGEITVTLQDVNILLGLRVDGPAVTGSTK---YNWADLCEDLLGHRPGPKDL-----H 152
Query: 61 GGYISYPRLRDFYTSYLGRANVLAGTEDPEEVEELERVRTYC-VRCYLLYLVGCLLFGDR 119
G ++S LR+ + + DP+EV C R ++L L+ L+GD+
Sbjct: 153 GSHVSLAWLRENFRNL---------PADPDEVT------LKCHTRAFVLALMSGFLYGDK 197
Query: 120 SNKRIELIYLTTMADGYAGMRNYSWGAMTLAYLYGELADACRLGHRALGGSVTLLTAWFL 179
S + L +L + D + + SWG+ TLA LY EL A + + G + LL W
Sbjct: 198 SKHDVALTFLPLLRD-FDEVAKLSWGSATLALLYRELCRASKRTVSTICGPLVLLQLWAW 256
Query: 180 AHF----PGFFSVDLNTDYLENYS------VAARWKL---QKGHGEGITYRSLLDRIQLD 226
PG D+ Y++ + R L + G YR D+ + +
Sbjct: 257 ERLHVGRPGRLK-DVGASYMDGIDGPLPDPLGCRASLSHKENPRGGLDFYRDQFDQQKDE 315
Query: 227 DVCWRSY-----EEHREIQDFEEVFWYSRWIMCGVRRVYRHLPERVLRQYGYVQTIP 278
V W+ Y + I E W + + V H P+RVLRQ+G QTIP
Sbjct: 316 QVIWQPYTPDLLAKIPLICVSGENIWRTVAPLICFDVVEWHRPDRVLRQFGLHQTIP 372
>At1g50760 hypothetical protein
Length = 649
Score = 32.0 bits (71), Expect = 0.53
Identities = 23/93 (24%), Positives = 37/93 (39%), Gaps = 22/93 (23%)
Query: 217 RSLLDRIQLDDVCWRSYEEHREIQDF------------------EEVFWYSRWI----MC 254
R +LD ++D WR Y F EE+ ++R I +
Sbjct: 296 RQILDNSKIDSFEWRPYTRSVTNWKFPQFYPEKAMCVHVSPSLDEELISFARCIKDSELV 355
Query: 255 GVRRVYRHLPERVLRQYGYVQTIPRHPTDVRDL 287
G+ +V + P RV Q+G +Q +P D +L
Sbjct: 356 GIEKVEHYFPNRVASQFGMLQDVPSPHVDRNNL 388
>At3g53180 nodulin / glutamate-ammonia ligase - like protein
Length = 845
Score = 29.6 bits (65), Expect = 2.6
Identities = 18/61 (29%), Positives = 30/61 (48%), Gaps = 1/61 (1%)
Query: 134 DGYAGMRNYSWGAMTLA-YLYGELADACRLGHRALGGSVTLLTAWFLAHFPGFFSVDLNT 192
DGYA Y GA ++ L+DAC G +L ++ F + GF+ ++++T
Sbjct: 328 DGYASPETYYLGAKKAREVIFLVLSDACASGDLSLMEAIDAAKDIFSRNSIGFYKLNIDT 387
Query: 193 D 193
D
Sbjct: 388 D 388
>At1g10680 putative P-glycoprotein-2 emb|CAA71277
Length = 1227
Score = 28.9 bits (63), Expect = 4.5
Identities = 34/120 (28%), Positives = 56/120 (46%), Gaps = 21/120 (17%)
Query: 40 LLMTY-LGVAQHEAEKICNQEYGGYISYPRLRDFYTSYLGRANVLAGTEDPEEVEELERV 98
+L TY L ++ H +EKI Q YGG +S +YL +AN+LAG E + + V
Sbjct: 810 VLATYPLIISGHISEKIFMQGYGGNLS--------KAYL-KANMLAG----ESISNIRTV 856
Query: 99 RTYCV--RCYLLYLVGCLLFGDRSNKRIELIYLTTMADGYAGMRNYSWGAMTLAYLYGEL 156
+C + LY L +RS +R ++ + Y + + + + LA YG +
Sbjct: 857 VAFCAEEKVLDLYSKELLEPSERSFRRGQMAGIL-----YGVSQFFIFSSYGLALWYGSI 911
>At4g08710 putative protein
Length = 715
Score = 28.1 bits (61), Expect = 7.7
Identities = 21/84 (25%), Positives = 39/84 (46%), Gaps = 6/84 (7%)
Query: 89 PEEVEELERVRTYCVRCYLLYLVGCLLFGDRSNKRIELIYLTTMADGYAGMRNYSWGAMT 148
PE ER+R + ++ + GD++ +++ +L M+D RN+ WG +
Sbjct: 326 PEGERSKERLRMMVLYFLSSIIIAPIKTGDKA-PQVDEFFLKVMSD-LTFCRNFQWGRYS 383
Query: 149 LAYLYGELADACRLGHRALGGSVT 172
Y+ G ++ + H GSVT
Sbjct: 384 FDYMLGTISHT--VNH--FNGSVT 403
>At1g50750 hypothetical protein
Length = 816
Score = 28.1 bits (61), Expect = 7.7
Identities = 17/64 (26%), Positives = 32/64 (49%), Gaps = 5/64 (7%)
Query: 217 RSLLDRIQLDDVCWRSYEEHREIQ--DFEEVFWYSRWIMCGVRRVYRHLPERVLRQYGYV 274
R +LD +++D V W + +++ F S+ + G+ V + P RV Q+G +
Sbjct: 258 RQILDSLKIDTV-WIPASPNLDVEFVSFARCIKVSQLV--GIDNVEHYFPNRVASQFGML 314
Query: 275 QTIP 278
Q +P
Sbjct: 315 QDVP 318
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.323 0.140 0.447
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,666,130
Number of Sequences: 26719
Number of extensions: 379936
Number of successful extensions: 756
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 739
Number of HSP's gapped (non-prelim): 12
length of query: 357
length of database: 11,318,596
effective HSP length: 100
effective length of query: 257
effective length of database: 8,646,696
effective search space: 2222200872
effective search space used: 2222200872
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 61 (28.1 bits)
Medicago: description of AC125478.7