BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125477.1 + phase: 2 /pseudo/partial
(358 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g11710 lysyl-tRNA synthetase 515 e-146
At3g13490 lysyl-tRNA synthetase like protein 209 2e-54
At4g33760 aspartate--tRNA ligase like protein 65 4e-11
At4g26870 putative aspartate-tRNA ligase 45 6e-05
At4g31180 aspartate--tRNA ligase - like protein 36 0.028
At3g02760 putative histidyl tRNA synthetase 35 0.083
At3g12240 hypothetical protein 31 1.2
At1g17580 myosin 30 1.6
At5g06580 unknown protein 30 2.0
At3g12203 serine carboxypeptidase, putative 30 2.0
At4g20140 leucine rich repeat-like protein 30 2.7
At1g17000 hypothetical protein 29 3.5
At5g19040 tRNA isopentenyltransferase -like protein 29 4.5
At3g62010 putative protein 29 4.5
At1g72410 hypothetical protein 29 4.5
At5g13610 unknown protein 28 5.9
At1g70010 hypothetical protein 28 5.9
At3g22670 hypothetical protein 28 7.7
At3g12230 hypothetical protein 28 7.7
>At3g11710 lysyl-tRNA synthetase
Length = 626
Score = 515 bits (1327), Expect = e-146
Identities = 255/376 (67%), Positives = 303/376 (79%), Gaps = 20/376 (5%)
Query: 2 SDLDEVEFCKFHSTVERGDTVGVIGFPGKSEEGDLSIFPKTFTVLSHCLHLMPDQKSTAA 61
S LDE EF K HS +RGD VGVIGFPGK++ G+LSIFP++F +LSHCLH+MP +
Sbjct: 177 SGLDEAEFLKLHSNAKRGDIVGVIGFPGKTKRGELSIFPRSFILLSHCLHMMPRKADNVN 236
Query: 62 AAA-----------NNANLMES*NIHFK--------GSDIREIFHTRNRIISYIQRHLDD 102
A A +++ ++ ++R+IF TR +IISY++R LD+
Sbjct: 237 AKKPEIWVPGQTRNPEAYVLKDQESRYRQRHLDMILNVEVRQIFRTRAKIISYVRRFLDN 296
Query: 103 LDFMEVETPMMNKIAGGAAARPFVTHHNELDLKLFMRIAPELYLKQLVVGGMERVYEIGK 162
+F+EVETPMMN IAGGAAARPFVTHHN+LD++L+MRIAPELYLKQL+VGG+ERVYEIGK
Sbjct: 297 KNFLEVETPMMNMIAGGAAARPFVTHHNDLDMRLYMRIAPELYLKQLIVGGLERVYEIGK 356
Query: 163 QFRNEDIDLTHNPEFTTCEFYMAYNEYNDLMEITERMLSGLVQELTNGSYKIKYHANGVD 222
QFRNE IDLTHNPEFTTCEFYMA+ +YNDLME+TE MLSG+V+ELT G YKIKY+ANG D
Sbjct: 357 QFRNEGIDLTHNPEFTTCEFYMAFADYNDLMEMTEVMLSGMVKELT-GGYKIKYNANGYD 415
Query: 223 KDPIEIDFTPPFRRIDMIEELESMAGLSIPKDLATDEANQYLKNACLEYDIKCAPAQTTA 282
KDPIEIDFTPPFRRI+MI ELE +A L+IPKDLA++EAN+YL +AC +D+KC P QTTA
Sbjct: 416 KDPIEIDFTPPFRRIEMIGELEKVAKLNIPKDLASEEANKYLIDACARFDVKCPPPQTTA 475
Query: 283 RLLDKLAGHFLEETCVNPTFIINHPEIMSPLAKWHRTKPGLTERFELIVNKLKLSNAYTE 342
RLLDKL G FLE TCVNPTFIIN PEIMSPLAKWHR+K GLTERFEL +NK +L NAYTE
Sbjct: 476 RLLDKLVGEFLEPTCVNPTFIINQPEIMSPLAKWHRSKSGLTERFELFINKHELCNAYTE 535
Query: 343 LNDPVVQRQRFAEQLK 358
LNDPVVQRQRFA+QLK
Sbjct: 536 LNDPVVQRQRFADQLK 551
>At3g13490 lysyl-tRNA synthetase like protein
Length = 602
Score = 209 bits (531), Expect = 2e-54
Identities = 129/361 (35%), Positives = 205/361 (56%), Gaps = 16/361 (4%)
Query: 4 LDEVEFCKFHSTVERGDTVGVIGFPGKSEEGDLSIFPKTFTVLSHCLHLMPDQKSTAAAA 63
L + +F + ++ GD +G G ++E+G+LSI +F++L+ L +PD+
Sbjct: 172 LSDDQFEQLKQFIDIGDILGASGSMKRTEKGELSICVNSFSILTKSLLPLPDKYHGLTDI 231
Query: 64 ANNANLMES*NIHFKGSDIREIFHTRNRIISYIQRHLDDLDFMEVETPMMNKIAGGAAAR 123
I ++ ++F R +I+S I++ ++ ++EVETP++ AGGA AR
Sbjct: 232 DKRYRQRYVDMI--ANPEVADVFRRRAKIVSEIRKTVESFGYLEVETPVLQGAAGGAEAR 289
Query: 124 PFVTHHNELDLKLFMRIAPELYLKQLVVGGMERVYEIGKQFRNEDIDLTHNPEFTTCEFY 183
PFVT HN L L++RIA EL+LK+++VGG E+VYEIG+ FRNE I HNPEFTT E Y
Sbjct: 290 PFVTFHNSLGRDLYLRIATELHLKRMLVGGFEKVYEIGRIFRNEGISTRHNPEFTTIEMY 349
Query: 184 MAYNEYNDLMEITERMLSGLVQELTNGSYKIKYHANGVDKDPIEIDFTPPFRRIDMIEEL 243
AY++Y+ +M++ E +++ + NG I Y EI P+RR M +
Sbjct: 350 EAYSDYHSMMDMAELIVTQCSMAV-NGKLTIDYQGT-------EICLERPWRRETMHNLV 401
Query: 244 ESMAGLS---IPKDL--ATDEANQYLKNACLEYDIKCAPA-QTTARLLDKLAGHFLEETC 297
+ + G++ + +DL A D L++ D A + LL+++ +E
Sbjct: 402 KEITGINFSELGEDLGNAKDTVLLALQDVLEPKDKSGIRACSSLGHLLNEIFEVVVEPKL 461
Query: 298 VNPTFIINHPEIMSPLAKWHRTKPGLTERFELIVNKLKLSNAYTELNDPVVQRQRFAEQL 357
V PTF++++P +SPLAK HR GLTERFEL + +++NA++EL DPV QR R EQ+
Sbjct: 462 VQPTFVLDYPIEISPLAKPHRGNAGLTERFELFICGREMANAFSELTDPVDQRTRLEEQV 521
Query: 358 K 358
+
Sbjct: 522 R 522
>At4g33760 aspartate--tRNA ligase like protein
Length = 664
Score = 65.5 bits (158), Expect = 4e-11
Identities = 32/120 (26%), Positives = 67/120 (55%), Gaps = 1/120 (0%)
Query: 89 RNRIISYIQRHLDDLD-FMEVETPMMNKIAGGAAARPFVTHHNELDLKLFMRIAPELYLK 147
R+ ++ I+R+L+D F+E+ETP++++ A V + + +P+L+ +
Sbjct: 222 RHNVVKLIRRYLEDRHGFIEIETPILSRSTPEGARDYLVPSRIQSGTFYALPQSPQLFKQ 281
Query: 148 QLVVGGMERVYEIGKQFRNEDIDLTHNPEFTTCEFYMAYNEYNDLMEITERMLSGLVQEL 207
L+V G ++ Y+I + FR+ED+ PEFT + MA+ D++++ E ++ + E+
Sbjct: 282 MLMVSGFDKYYQIARCFRDEDLRADRQPEFTQLDMEMAFMPMEDMLKLNEDLIRKVFSEI 341
>At4g26870 putative aspartate-tRNA ligase
Length = 532
Score = 45.1 bits (105), Expect = 6e-05
Identities = 52/212 (24%), Positives = 89/212 (41%), Gaps = 28/212 (13%)
Query: 7 VEFCKFHSTVERGDTVGVIGFPGKSEEG---DLSIFPKTFTVLSHCLHLMPDQKSTAAAA 63
++F K S + +GV+ P K G + I + LS L +P AA +
Sbjct: 129 IKFVKQLSRESVVELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPLVVEDAARS 188
Query: 64 ANN-----------ANLMES*NIHFKGSDIRE-----IFHTRNRIISYIQRHLDDLDFME 107
++ A +++ ++ + DIR IF + ++ + +L F+E
Sbjct: 189 ESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREYLQSKGFLE 248
Query: 108 VETPMMNKIAGGAAARPFVTHHNELDLKLFMRIAPELYLKQLVVGGMERVYEIGKQFRNE 167
+ TP + IAG + V + + +P+L+ + + G M RV+E+G FR E
Sbjct: 249 IHTPKL--IAGSSEGGSAVFRLDYKGQPACLAQSPQLHKQMAICGDMRRVFEVGPVFRAE 306
Query: 168 DIDLTHNP--EF----TTCEFYMAYNEYNDLM 193
D TH EF E M Y+E DL+
Sbjct: 307 D-SFTHRHLCEFVGLDVEMEIRMHYSEIMDLV 337
>At4g31180 aspartate--tRNA ligase - like protein
Length = 558
Score = 36.2 bits (82), Expect = 0.028
Identities = 37/173 (21%), Positives = 72/173 (41%), Gaps = 7/173 (4%)
Query: 85 IFHTRNRIISYIQRHLDDLDFMEVETPMMNKIAGGAAARPFVTHHNELDLKLFMRIAPEL 144
IF + + + L +F+ + TP + +AG + V + +P+L
Sbjct: 252 IFQLQYEVEYAFREKLRFKNFVGIHTPKL--MAGSSEGGSAVFRLEYKGQPACLAQSPQL 309
Query: 145 YLKQLVVGGMERVYEIGKQFRNEDIDLTHNP--EFTTCEFYMAYNE-YNDLMEITERMLS 201
+ + + G + RV+E+G FR ED TH EF + M + Y+++M++ + +
Sbjct: 310 HKQMAICGDLRRVFEVGPVFRAED-SFTHRHLCEFVGLDVEMEIRKHYSEIMDLVDELFV 368
Query: 202 GLVQELTNGSYKIKYHANGVDKDPIEIDFTPPFRRIDMIEELESMAGLSIPKD 254
+ L N K + A G + F P R+ E ++ + + D
Sbjct: 369 FIFTSL-NERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVD 420
>At3g02760 putative histidyl tRNA synthetase
Length = 479
Score = 34.7 bits (78), Expect = 0.083
Identities = 28/107 (26%), Positives = 53/107 (49%), Gaps = 6/107 (5%)
Query: 158 YEIGKQFRNEDIDLTHNPEFTTCEFYMA--YNEYNDLMEITERMLSGLVQELTNGSYKIK 215
Y+I K +R ++ EF C+F +A + EI ++L+ L+ EL G Y++K
Sbjct: 136 YQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIV-KILTELLDELEIGDYEVK 194
Query: 216 YHANGVDKDPIEIDFTPP--FRRI-DMIEELESMAGLSIPKDLATDE 259
+ + +EI PP FR I I++L+ + + K++ ++
Sbjct: 195 LNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEK 241
>At3g12240 hypothetical protein
Length = 449
Score = 30.8 bits (68), Expect = 1.2
Identities = 17/61 (27%), Positives = 34/61 (54%), Gaps = 12/61 (19%)
Query: 152 GGMERVYEIGKQFRNEDIDLTHNPEFTTCEFYMAYNEYNDLMEITERMLSGLVQELTNGS 211
G ++RV E +++ L +PE+ + FY+ N Y+ +++ +VQE++NG+
Sbjct: 149 GSVKRVNEFVRKW------LAKHPEYFSNPFYVTGNSYSG------KVIPAIVQEISNGN 196
Query: 212 Y 212
Y
Sbjct: 197 Y 197
>At1g17580 myosin
Length = 1520
Score = 30.4 bits (67), Expect = 1.6
Identities = 18/72 (25%), Positives = 38/72 (52%), Gaps = 4/72 (5%)
Query: 218 ANGVDKDPIEIDFTPPFRRIDMIE-ELESMAGLSIPKDLATDEANQYLKNACLEYDIKCA 276
A+ V+K+P+ ++ T +ID + E++ + GL + DEA ++A ++ + C
Sbjct: 955 ASSVNKEPVVVEDT---EKIDSLSNEIDRLKGLLSSETHKADEAQHAYQSALVQNEELCK 1011
Query: 277 PAQTTARLLDKL 288
+ R +D+L
Sbjct: 1012 KLEEAGRKIDQL 1023
>At5g06580 unknown protein
Length = 567
Score = 30.0 bits (66), Expect = 2.0
Identities = 22/100 (22%), Positives = 39/100 (39%)
Query: 16 VERGDTVGVIGFPGKSEEGDLSIFPKTFTVLSHCLHLMPDQKSTAAAAANNANLMES*NI 75
+ + V V FP + D++I + + L+ + + A AN NL E+ +
Sbjct: 319 IPQHSVVAVCNFPTVKDAADVAIATMMSGIQVSRVELLDEVQIRAINMANGKNLTEAPTL 378
Query: 76 HFKGSDIREIFHTRNRIISYIQRHLDDLDFMEVETPMMNK 115
F+ + +I+ I + DFM E P K
Sbjct: 379 MFEFIGTEAYTREQTQIVQQIASKHNGSDFMFAEEPEAKK 418
>At3g12203 serine carboxypeptidase, putative
Length = 437
Score = 30.0 bits (66), Expect = 2.0
Identities = 31/112 (27%), Positives = 48/112 (42%), Gaps = 16/112 (14%)
Query: 171 LTHNPEFTTCEFYMAYNEYNDLMEITERMLSGLVQELTNGSYKIKYHANGVDKDP-IEID 229
L +PEF + Y+A N Y+ + ++ +VQE++NG N +D P I +
Sbjct: 164 LDKHPEFLSNPLYVAGNSYSGI------VIPTIVQEISNG--------NHLDSKPQINLQ 209
Query: 230 -FTPPFRRIDMIEELESMAGLSIPKDLATDEANQYLKNACLEYDIKCAPAQT 280
F D +L S + K L +DE + LK +C I P T
Sbjct: 210 GFVLGNPATDTDIDLNSRIPFAHGKALISDEHYESLKRSCQGNYISVNPRNT 261
>At4g20140 leucine rich repeat-like protein
Length = 1232
Score = 29.6 bits (65), Expect = 2.7
Identities = 34/122 (27%), Positives = 54/122 (43%), Gaps = 19/122 (15%)
Query: 146 LKQLVVGGMERVYEIGKQFRN----EDIDLTHN------PE--FTTCEFYMAYNEYNDLM 193
L+QLV+ G + EI + + +DL++N PE F E Y N L
Sbjct: 338 LEQLVLSGTQLSGEIPVELSKCQSLKQLDLSNNSLAGSIPEALFELVELTDLYLHNNTL- 396
Query: 194 EITERMLSGLVQELTNGSYKIKYHANGVDKDPIEIDFTPPFRRIDMIEELESMAGLSIPK 253
E LS + LTN + + YH N K P EI R+++++ E+ IP+
Sbjct: 397 ---EGTLSPSISNLTNLQWLVLYHNNLEGKLPKEIS---ALRKLEVLFLYENRFSGEIPQ 450
Query: 254 DL 255
++
Sbjct: 451 EI 452
>At1g17000 hypothetical protein
Length = 730
Score = 29.3 bits (64), Expect = 3.5
Identities = 19/63 (30%), Positives = 29/63 (45%), Gaps = 3/63 (4%)
Query: 164 FRNEDIDLTHNPEFTTCEFYMAYNEYNDLME-ITERMLSGLVQELTNGSYKIKYHANGVD 222
F L N E+ EF A + DL++ + +S E+ G Y ++ HA GV
Sbjct: 547 FEASKTSLVWNYEYADVEFGRA--QARDLLQYLWAGPISNASAEVVRGKYSVEVHAIGVT 604
Query: 223 KDP 225
K+P
Sbjct: 605 KEP 607
>At5g19040 tRNA isopentenyltransferase -like protein
Length = 330
Score = 28.9 bits (63), Expect = 4.5
Identities = 27/107 (25%), Positives = 46/107 (42%), Gaps = 8/107 (7%)
Query: 221 VDKDPIEIDFTPPFRRIDMIEELESMAGLSIPKDLATDEANQYLKNACLEYDIKCAPAQT 280
VDK +++ RRI + AG I + + E +++L++ Y PA+T
Sbjct: 172 VDK-MVDMGLVDEVRRIFDPSSSDYSAG--IRRAIGVPELDEFLRSEMRNY-----PAET 223
Query: 281 TARLLDKLAGHFLEETCVNPTFIINHPEIMSPLAKWHRTKPGLTERF 327
T RLL+ E TC+ + + + KW+ + TE F
Sbjct: 224 TERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEVF 270
>At3g62010 putative protein
Length = 1254
Score = 28.9 bits (63), Expect = 4.5
Identities = 20/70 (28%), Positives = 31/70 (43%), Gaps = 12/70 (17%)
Query: 2 SDLDEVEFCKFHSTVERGDTVGVIGFPGKSEEGDLSIFPKTFTVLSHCLHLMPDQKSTAA 61
SD+D +++CK D+ G F + G +S + + CL L P Q TA
Sbjct: 1069 SDVDAIQYCK-------DDSAGSYLFVSHTGFGGVS-----YAMAITCLLLQPGQNFTAT 1116
Query: 62 AAANNANLME 71
N++ L E
Sbjct: 1117 PTTNSSTLEE 1126
>At1g72410 hypothetical protein
Length = 122
Score = 28.9 bits (63), Expect = 4.5
Identities = 15/56 (26%), Positives = 30/56 (52%), Gaps = 6/56 (10%)
Query: 174 NPEFTTCEFYMAYNEYNDLMEITERMLSGLVQELTNGSYKIKYHANGVDKDPIEID 229
+P+ + CE +++ NE TE++ SGL+Q N ++ A+ V + I ++
Sbjct: 15 SPKHSKCELFVSSNEQ------TEKLASGLIQPFVNHLKVLEAKASPVAQSSIRLE 64
>At5g13610 unknown protein
Length = 402
Score = 28.5 bits (62), Expect = 5.9
Identities = 20/78 (25%), Positives = 37/78 (46%), Gaps = 9/78 (11%)
Query: 182 FYMAYNEYNDLMEITERMLSGLVQELTNGSYKIKYHAN-----GVDKDPIEIDFTPPFRR 236
F + +E ++ +++ ER SGL+ E+ Y+++ + N V +D I + F
Sbjct: 193 FNVREHEVDEYLKVVERHASGLLPEMRKDEYEVRENPNLDTWMEVGRDFIRLQFL----N 248
Query: 237 IDMIEELESMAGLSIPKD 254
D I + + G SI D
Sbjct: 249 TDGIRTIGCVLGQSIALD 266
>At1g70010 hypothetical protein
Length = 1315
Score = 28.5 bits (62), Expect = 5.9
Identities = 17/51 (33%), Positives = 31/51 (60%), Gaps = 4/51 (7%)
Query: 304 INHPEIMSPLAKWHRTKP--GLTERFELIVNKLKLSNAYT--ELNDPVVQR 350
I++ E SP+AK + K G+ RF+L + +L +SNA+ +L++ + R
Sbjct: 876 IDYNETFSPVAKLNSVKLLLGVAARFKLSLTQLDISNAFLNGDLDEEIYMR 926
>At3g22670 hypothetical protein
Length = 562
Score = 28.1 bits (61), Expect = 7.7
Identities = 31/130 (23%), Positives = 51/130 (38%), Gaps = 11/130 (8%)
Query: 106 MEVETPMMNKIAGGAAARPFVTHHNELDLKLFMRIAPELYLKQLVVGGMERVYEIGKQFR 165
++ +T MN + + H +E+ LKLF I P+ +++ G + +
Sbjct: 235 VKTDTIAMNSLMDALVKENSIEHAHEVFLKLFDTIKPDARTFNILIHGFCKARKFDD--A 292
Query: 166 NEDIDLTHNPEFTT-----CEFYMAYNEYNDLMEITERMLSGLVQELTNG---SYKIKYH 217
+DL EFT F AY + D + E ML + + N +Y I H
Sbjct: 293 RAMMDLMKVTEFTPDVVTYTSFVEAYCKEGDFRRVNE-MLEEMRENGCNPNVVTYTIVMH 351
Query: 218 ANGVDKDPIE 227
+ G K E
Sbjct: 352 SLGKSKQVAE 361
>At3g12230 hypothetical protein
Length = 435
Score = 28.1 bits (61), Expect = 7.7
Identities = 12/42 (28%), Positives = 25/42 (58%), Gaps = 6/42 (14%)
Query: 171 LTHNPEFTTCEFYMAYNEYNDLMEITERMLSGLVQELTNGSY 212
L +P++ + FY+ N Y+ +++ +VQE++NG+Y
Sbjct: 161 LAKHPDYFSNPFYVTGNSYSG------KVIPAIVQEISNGNY 196
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.138 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,077,367
Number of Sequences: 26719
Number of extensions: 342887
Number of successful extensions: 717
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 704
Number of HSP's gapped (non-prelim): 21
length of query: 358
length of database: 11,318,596
effective HSP length: 100
effective length of query: 258
effective length of database: 8,646,696
effective search space: 2230847568
effective search space used: 2230847568
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC125477.1