BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124972.5 - phase: 0
(222 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g09620 putative protein 114 5e-26
At5g64430 unknown protein 110 6e-25
At5g63130 unknown protein 82 3e-16
At5g49920 unknown protein 82 3e-16
At4g05150 unknown protein 80 1e-15
At2g01190 unknown protein 78 3e-15
At3g18230 unknown protein 71 4e-13
At1g79570 unknown protein 71 5e-13
At5g57610 putative protein 70 7e-13
At3g46920 protein kinase - like protein 70 9e-13
At1g16270 putative Ser/Thr protein kinase 68 3e-12
At3g26510 unknown protein 68 4e-12
At3g24715 hypothetical protein, 3' partial 67 1e-11
At1g25300 67 1e-11
At2g35050 putative protein kinase 65 3e-11
At1g04700 putative Ser/Thr protein kinase 63 1e-10
At1g70640 unknown protein 62 2e-10
At3g48240 putative protein 61 5e-10
At5g16220 unknown protein 46 2e-05
At1g69690 unknown protein 35 0.041
>At5g09620 putative protein
Length = 531
Score = 114 bits (284), Expect = 5e-26
Identities = 72/152 (47%), Positives = 94/152 (61%), Gaps = 13/152 (8%)
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFS---- 58
+VKLM +YGGKIQPR HD+ + +Y+ GD KI++VDR I F L++KLS
Sbjct: 39 KVKLMCSYGGKIQPRP---HDN-QLTYVNGDTKIMSVDRGIRFPALVSKLSAVCSGGGDG 94
Query: 59 -DVCFKYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLFP-VPINNNK 116
++ FKYQL GED DALI + N+EDL H+M EYDR+ S KPA +R+FLFP PI+
Sbjct: 95 GEISFKYQLPGEDLDALISVTNDEDLEHMMHEYDRLLRLSTKPARMRLFLFPSSPISGGF 154
Query: 117 ASFDSLASVK-SLNFVRVSPFEEYSSPPTTPP 147
S S S + +LN + P E S T PP
Sbjct: 155 GSEGSTKSDRDTLNPIPSRPESEKS--VTAPP 184
>At5g64430 unknown protein
Length = 513
Score = 110 bits (275), Expect = 6e-25
Identities = 72/170 (42%), Positives = 98/170 (57%), Gaps = 27/170 (15%)
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFS---- 58
+VK M +YGGKIQPR HD+ + +Y+ G+ KI++VDR I F L +KLST
Sbjct: 44 KVKFMCSYGGKIQPRP---HDN-QLTYVNGETKILSVDRGIRFPVLASKLSTVCGGGDGG 99
Query: 59 --DVCFKYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLFPVPI---N 113
+V FKYQL GED DALI + N++DL H+M EYDR+ S KPA +R+FLFP +
Sbjct: 100 GGEVTFKYQLPGEDLDALISVTNDDDLEHMMHEYDRLLRLSSKPARMRLFLFPASSGFGS 159
Query: 114 NNKASFDSLASVKSLNFV-RVSPFEE-YSSPP------------TTPPPP 149
+ D V++LN V R+S E+ ++PP PPPP
Sbjct: 160 QSSTQSDRDRFVEALNTVPRLSESEKSVTAPPNNADFLFGSEKVAAPPPP 209
>At5g63130 unknown protein
Length = 192
Score = 81.6 bits (200), Expect = 3e-16
Identities = 52/147 (35%), Positives = 79/147 (53%), Gaps = 13/147 (8%)
Query: 4 VKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDVCFK 63
+K + +YGG+I PR T + Y+GG ++++VDR+I+FS+LM KL F V +
Sbjct: 15 LKFLCSYGGRILPRST----DGKLRYVGGHTRVLSVDRSISFSELMKKLYEFCGYSVDLR 70
Query: 64 YQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLFPVPINNNKASFDSLA 123
QL D + LI + +EE+L I+ EYDR+C A +R L P ++ S S +
Sbjct: 71 CQLPNGDLETLISVKSEEELAEIVEEYDRIC-----GAKIRAVLSPPRSSHKTESSPSSS 125
Query: 124 SVKSLNFVRVSPFEEYSSPPTTPPPPF 150
+S SPF SPP +P P +
Sbjct: 126 GDRSPK----SPFSVTPSPPNSPSPAY 148
>At5g49920 unknown protein
Length = 288
Score = 81.6 bits (200), Expect = 3e-16
Identities = 43/107 (40%), Positives = 71/107 (66%), Gaps = 9/107 (8%)
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDVCF 62
+VK M ++GG+I PR + D + Y+GG+ +++ V +I+FS+LM KL+ +D+
Sbjct: 9 KVKFMCSFGGRILPRPS---DSV-LKYVGGETRVVAVSPDISFSELMKKLTAITENDIVL 64
Query: 63 KYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLFP 109
KYQ++ ED DAL+ + ++ED+ H++ EY+R H + K LR FLFP
Sbjct: 65 KYQIIPEDLDALVSVKSDEDVKHMIEEYNR--HETPK---LRTFLFP 106
>At4g05150 unknown protein
Length = 477
Score = 79.7 bits (195), Expect = 1e-15
Identities = 46/128 (35%), Positives = 77/128 (59%), Gaps = 6/128 (4%)
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMF-SDVC 61
RV+ M T+GG+I PR + Y+GGDN+++ V R+ F+ L++KL+ S++
Sbjct: 58 RVRFMCTFGGRILPR----PPDNQLCYVGGDNRMVAVHRHTTFASLLSKLAKLSGKSNIS 113
Query: 62 FKYQLLGEDFDALIPIYNEEDLNHIMFEYDRMC-HFSRKPAWLRVFLFPVPINNNKASFD 120
KYQL ED DALI + +ED+ ++M EYDR+ + + + + LR+FLF + + +
Sbjct: 114 VKYQLPNEDLDALISVSTDEDVENMMDEYDRVAQNQNPRASRLRLFLFTKNVAGEEDNDS 173
Query: 121 SLASVKSL 128
+S+ SL
Sbjct: 174 RASSISSL 181
>At2g01190 unknown protein
Length = 720
Score = 78.2 bits (191), Expect = 3e-15
Identities = 47/110 (42%), Positives = 67/110 (60%), Gaps = 8/110 (7%)
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDVCF 62
+++ M +YGG I PR HD Y+GGD +I+ VDRN + L+A+LS + F
Sbjct: 74 KLRFMCSYGGHILPRP---HDK-SLCYMGGDTRIVVVDRNSSLPSLIARLSNTLLDGRSF 129
Query: 63 --KYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSR--KPAWLRVFLF 108
KYQL ED D+LI + +EDL++++ EYDR S KP+ LR+FLF
Sbjct: 130 TLKYQLPSEDLDSLISVTTDEDLDNMIEEYDRTISASNSTKPSRLRLFLF 179
>At3g18230 unknown protein
Length = 666
Score = 71.2 bits (173), Expect = 4e-13
Identities = 43/110 (39%), Positives = 66/110 (59%), Gaps = 8/110 (7%)
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDVCF 62
+++LM ++GG I PR HD +Y GG+ +I+ VDR + S L ++LS+ + + F
Sbjct: 63 KLRLMCSFGGHIMPRP---HDK-SLTYSGGETRIVVVDRRASLSSLRSRLSSMLLNGRSF 118
Query: 63 --KYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAW--LRVFLF 108
KYQL ED D+L+ I +EDL +++ EYDR + A LR+FLF
Sbjct: 119 TLKYQLPSEDLDSLVTITTDEDLENMIEEYDRAASSATATATQRLRLFLF 168
>At1g79570 unknown protein
Length = 1248
Score = 70.9 bits (172), Expect = 5e-13
Identities = 38/108 (35%), Positives = 65/108 (60%), Gaps = 5/108 (4%)
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDVCF 62
+VK++ ++GGKI PR + Y+GG+ II++ ++I++ +L K+ +
Sbjct: 176 KVKILCSFGGKILPR----PGDSKLRYVGGETHIISIRKDISWQELRQKILEIYYQTRVV 231
Query: 63 KYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLFPV 110
KYQL GED DAL+ + +EEDL +++ EY+ M + LR+FLF +
Sbjct: 232 KYQLPGEDLDALVSVSSEEDLQNMLEEYNEMENRGGSQK-LRMFLFSI 278
>At5g57610 putative protein
Length = 1054
Score = 70.5 bits (171), Expect = 7e-13
Identities = 39/112 (34%), Positives = 65/112 (57%), Gaps = 11/112 (9%)
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDVCF 62
RVK + ++ G I PR + Y+GG+ +I++V+R+I + +LM+K+
Sbjct: 23 RVKFLCSFLGSILPR----PQDGKLRYVGGETRIVSVNRDIRYEELMSKMRELYDGAAVL 78
Query: 63 KYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCH---FSRKPAWLRVFLFPVP 111
KYQ ED DAL+ + N++D+ ++M EYD++ F+R LR+FLF P
Sbjct: 79 KYQQPDEDLDALVSVVNDDDVTNMMEEYDKLGSGDGFTR----LRIFLFSTP 126
>At3g46920 protein kinase - like protein
Length = 1171
Score = 70.1 bits (170), Expect = 9e-13
Identities = 43/123 (34%), Positives = 69/123 (55%), Gaps = 5/123 (4%)
Query: 1 MERVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDV 60
+E+VK + +Y GKI PR + LRY +GG +I++V +N+ F + K+ V
Sbjct: 72 LEKVKFLCSYNGKIIPRPS--DGMLRY--VGGQTRIVSVKKNVRFDEFEQKMIQVYGHPV 127
Query: 61 CFKYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAW-LRVFLFPVPINNNKASF 119
KYQL ED DAL+ + + ED++++M E++++ S + LRVFLF + SF
Sbjct: 128 VVKYQLPDEDLDALVSVSSSEDIDNMMEEFEKLVERSSDGSGKLRVFLFDASSSEVDDSF 187
Query: 120 DSL 122
L
Sbjct: 188 GIL 190
>At1g16270 putative Ser/Thr protein kinase
Length = 1147
Score = 68.2 bits (165), Expect = 3e-12
Identities = 43/125 (34%), Positives = 70/125 (55%), Gaps = 11/125 (8%)
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDVCF 62
+VK++ ++GGKI PR + Y+GG+ II++ ++I++ +L K+ +
Sbjct: 164 KVKVLCSFGGKILPR----PGDSKLRYVGGETHIISIRKDISWQELRQKVLEIYYRTHVV 219
Query: 63 KYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLFPVP------INNNK 116
KYQL GED DAL+ + +EDL ++M EY+ M + LR+FLF V + NK
Sbjct: 220 KYQLPGEDLDALVSVSCDEDLLNMMEEYNEMENRGGSQK-LRMFLFSVSDLDGALLGVNK 278
Query: 117 ASFDS 121
+ DS
Sbjct: 279 SDVDS 283
>At3g26510 unknown protein
Length = 196
Score = 67.8 bits (164), Expect = 4e-12
Identities = 57/176 (32%), Positives = 85/176 (47%), Gaps = 34/176 (19%)
Query: 4 VKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFS----- 58
+K + +YGGKI PR LRY+ GG +++ V R+++FS+L +K++
Sbjct: 10 IKFLCSYGGKILPRYP--DGKLRYN--GGHTRVLAVPRSVSFSELASKMAEMCGGVGGSI 65
Query: 59 DVCFKYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLFP--------- 109
V + QL ED DAL+ I ++EDL +++ EYD + S P +RVFL P
Sbjct: 66 TVTIRCQLPTEDLDALVSITSDEDLVNLIEEYDLVS--SSSPMKIRVFLNPPKSAAGSKK 123
Query: 110 ------VPINNNKASFDSLASVKSLNFVRVSPFEEYSSPPTTPPPPFMATKDTVTK 159
+P + +S + +S S SP S P PP P T TVTK
Sbjct: 124 SPPPLALPSSTTTSSSSTTSSTSS------SPRSPSLSKPPLPPSPPRIT--TVTK 171
>At3g24715 hypothetical protein, 3' partial
Length = 800
Score = 66.6 bits (161), Expect = 1e-11
Identities = 38/117 (32%), Positives = 68/117 (57%), Gaps = 9/117 (7%)
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDVCF 62
+VK + ++GG+I PR T + Y+GG+ II++ +N+++ +L K S
Sbjct: 175 KVKFLCSFGGRIMPRST----DEKLKYVGGETHIISIRKNLSWEELKKKTSAICQQLHSI 230
Query: 63 KYQLLGEDFDALIPIYNEEDLNHIMFEYDRM--CHFSRKPAWLRVFLFPVPINNNKA 117
KYQL G++ D+LI + ++EDL +++ EY+ + S++P R+FL P+ KA
Sbjct: 231 KYQLPGDELDSLISVSSDEDLQNMIEEYNGLERLEGSQRP---RLFLIPIGEPERKA 284
>At1g25300
Length = 272
Score = 66.6 bits (161), Expect = 1e-11
Identities = 55/168 (32%), Positives = 83/168 (48%), Gaps = 20/168 (11%)
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDVCF 62
++ L+ +YGG+I P L YIGG+ +++ V R I+F D LS + S F
Sbjct: 10 KLHLLCSYGGRIMP----LPPEKSLHYIGGETRLVIVPRGISFLDFFKLLSDKLLSGRSF 65
Query: 63 --KYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLFP---VPINNNKA 117
KY+L DFD+LI + + EDL +++ EYD S + +R+FLFP V +
Sbjct: 66 SLKYKLPSCDFDSLITVSDNEDLQNMIAEYD-----STRLRRIRLFLFPLNHVELTRQSV 120
Query: 118 SFDSLASVKSLNFVRVSPFEEYSSP---PTTPPPPFM-ATKDTVTKAE 161
+ + L ++S F +S SSP P P F+ K T AE
Sbjct: 121 TVNRLLGLESPIFTSLS--SVLSSPIFAPNNGPVQFVHHNKVNATTAE 166
>At2g35050 putative protein kinase
Length = 1257
Score = 65.1 bits (157), Expect = 3e-11
Identities = 32/89 (35%), Positives = 52/89 (57%), Gaps = 4/89 (4%)
Query: 1 MERVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDV 60
+ R K + ++GGK+ PR + Y+GG+ +II + + I+F +LM K+
Sbjct: 174 LNRAKFLCSFGGKVIPRPR----DQKLRYVGGETRIIRISKTISFQELMHKMKEIFPEAR 229
Query: 61 CFKYQLLGEDFDALIPIYNEEDLNHIMFE 89
KYQL GED DAL+ + ++EDL ++M E
Sbjct: 230 TIKYQLPGEDLDALVSVSSDEDLQNMMEE 258
>At1g04700 putative Ser/Thr protein kinase
Length = 1042
Score = 62.8 bits (151), Expect = 1e-10
Identities = 38/108 (35%), Positives = 59/108 (54%), Gaps = 5/108 (4%)
Query: 2 ERVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDVC 61
E +KL+ ++GG+I R + YIGG+ +II++ +++ ++LM K
Sbjct: 120 EVLKLLCSFGGRILQR----PGDGKLRYIGGETRIISIRKHVGLNELMHKTYALCNHPHT 175
Query: 62 FKYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLFP 109
KYQL GED DALI + ++EDL H++ EY +RVFL P
Sbjct: 176 IKYQLPGEDLDALISVCSDEDLLHMIEEYQE-AETKAGSQRIRVFLVP 222
>At1g70640 unknown protein
Length = 174
Score = 62.4 bits (150), Expect = 2e-10
Identities = 51/150 (34%), Positives = 75/150 (50%), Gaps = 18/150 (12%)
Query: 4 VKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFM-FSDVCF 62
VK + +YGG+I PR LRY GGD ++++V R I+F++L KL +
Sbjct: 8 VKFLCSYGGRITPRYP--DGKLRYQ--GGDTRVLSVTRAISFTELKKKLGEICGIAVTSL 63
Query: 63 KYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLFPVPINNNKA----- 117
+ QL +D DAL+ + ++EDL ++M EYD K + VFL P+ A
Sbjct: 64 RCQLPTDDLDALVTVRSDEDLKNLMEEYDLAITAQVK---IHVFLSPLKSTRTTANSSPP 120
Query: 118 -SFDSLASVKSLNFVRVSPFEEYSSPPTTP 146
S S +S KS + SP S+P T P
Sbjct: 121 PSTTSSSSSKSRSRSPPSP----STPETCP 146
>At3g48240 putative protein
Length = 180
Score = 60.8 bits (146), Expect = 5e-10
Identities = 34/90 (37%), Positives = 56/90 (61%), Gaps = 4/90 (4%)
Query: 4 VKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFSDVCFK 63
+K + +YGG+I PR ++ LRY +GG ++++V +I+F++L KL F V K
Sbjct: 14 LKFLCSYGGRILPR--SIDGKLRY--VGGFTRVLSVHHSISFTELTMKLEEFCGYSVELK 69
Query: 64 YQLLGEDFDALIPIYNEEDLNHIMFEYDRM 93
QL D + LI + ++EDL +I+ EYDR+
Sbjct: 70 CQLPNGDLETLISVKSDEDLVNIVEEYDRV 99
>At5g16220 unknown protein
Length = 476
Score = 45.8 bits (107), Expect = 2e-05
Identities = 34/114 (29%), Positives = 62/114 (53%), Gaps = 11/114 (9%)
Query: 3 RVKLMITYGGKI-QPRLTALHDHLRYSYIGGDNKIITVDRNI--NFSDLMAKLSTFMFSD 59
++++M YGG I P T Y+GGD +I+ + + +F+ L++ L+ +
Sbjct: 30 KLRVMCRYGGSIVSPPQTKSP-----RYVGGDTRIVAIPSSAETSFASLVSHLTVTLKIS 84
Query: 60 VCFK--YQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKP-AWLRVFLFPV 110
FK YQL ++ D+LI + +ED+ +M E+ + S P + +R+FLFP+
Sbjct: 85 YPFKVKYQLPDQELDSLISVEADEDVQIMMEEHGYLSSESSIPQSRIRLFLFPL 138
>At1g69690 unknown protein
Length = 325
Score = 34.7 bits (78), Expect = 0.041
Identities = 21/58 (36%), Positives = 29/58 (49%), Gaps = 5/58 (8%)
Query: 109 PVPINNNKASF-----DSLASVKSLNFVRVSPFEEYSSPPTTPPPPFMATKDTVTKAE 161
P P +N++ +F DS S S + +S E +S P PPP +TKD TK E
Sbjct: 3 PDPDHNHRPNFPLQLLDSSTSSSSTSLAIISTTSEPNSEPKKPPPKRTSTKDRHTKVE 60
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.325 0.141 0.433
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,418,785
Number of Sequences: 26719
Number of extensions: 246704
Number of successful extensions: 1687
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 20
Number of HSP's that attempted gapping in prelim test: 1571
Number of HSP's gapped (non-prelim): 110
length of query: 222
length of database: 11,318,596
effective HSP length: 96
effective length of query: 126
effective length of database: 8,753,572
effective search space: 1102950072
effective search space used: 1102950072
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC124972.5