BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124972.11 - phase: 0 /pseudo
(127 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60345 unknown protein 184 1e-47
At2g37250 putative adenylate kinase 35 0.008
At1g63160 replication factor, putative 35 0.010
At4g24850 unknown protein (At4g24850) 33 0.039
At2g39270 putative adenylate kinase 33 0.051
At1g03000 putative peroxisome assembly factor-2 33 0.051
At4g02470 32 0.066
At4g25280 UMP/CMP kinase like protein 32 0.11
At1g02890 hypothetical protein 32 0.11
At5g53540 26S proteasome regulatory particle chain RPT6-like pro... 31 0.15
At4g24710 protein binding protein like 31 0.15
At1g77470 putative replication factor C 31 0.15
At1g21690 putative replication factor C subunit 31 0.15
At5g47010 UPF1 31 0.19
At4g29040 26S proteasome AAA-ATPase subunit RPT2a 30 0.25
At2g20140 26S proteasome subunit 4 30 0.25
At3g53230 CDC48 - like protein 30 0.33
At3g19740 ATPase, putative 30 0.33
At1g50140 hypothetical protein, 5' partial 30 0.33
At5g41120 unknown protein 30 0.43
>At5g60345 unknown protein
Length = 178
Score = 184 bits (466), Expect = 1e-47
Identities = 82/121 (67%), Positives = 104/121 (85%)
Query: 7 KRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYILNED 66
+R +PN+L+TGTPGTGK+TT++ALAEAT L +I IGDLVKEK + GWD+EL+ + +NED
Sbjct: 10 RRERPNLLITGTPGTGKSTTASALAEATNLRYICIGDLVKEKEFYHGWDNELECHFINED 69
Query: 67 LVCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRRGYKESKLSNN 126
V DEL+D M EGGNIVDYHGCDFFP+RWFD VVVL+T+N++LYDRL+ RGY +KLSNN
Sbjct: 70 SVIDELDDAMIEGGNIVDYHGCDFFPQRWFDRVVVLRTENSVLYDRLTNRGYSGTKLSNN 129
Query: 127 V 127
+
Sbjct: 130 L 130
>At2g37250 putative adenylate kinase
Length = 284
Score = 35.4 bits (80), Expect = 0.008
Identities = 24/67 (35%), Positives = 35/67 (51%), Gaps = 1/67 (1%)
Query: 7 KRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYILNE- 65
K R + G PG GK T ++ L+ + HI GDLV+E+ G + S I+N+
Sbjct: 48 KDRNVQWVFLGCPGVGKGTYASRLSTLLGVPHIATGDLVREELASSGPLSQKLSEIVNQG 107
Query: 66 DLVCDEL 72
LV DE+
Sbjct: 108 KLVSDEI 114
>At1g63160 replication factor, putative
Length = 333
Score = 35.0 bits (79), Expect = 0.010
Identities = 19/42 (45%), Positives = 26/42 (61%), Gaps = 2/42 (4%)
Query: 11 PNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHD 52
PN++++G PGTGKTT+ ALA +L N + V E N D
Sbjct: 49 PNLILSGPPGTGKTTSILALAH--ELLGTNYKEAVLELNASD 88
>At4g24850 unknown protein (At4g24850)
Length = 442
Score = 33.1 bits (74), Expect = 0.039
Identities = 19/60 (31%), Positives = 29/60 (47%), Gaps = 4/60 (6%)
Query: 3 KDSIKRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYI 62
K + + IL+ G PGTGKT + A+A+ N INI ++ W E + Y+
Sbjct: 168 KGELTKPCKGILLFGPPGTGKTMLAKAVAKEADANFINI----SMSSITSKWFGEGEKYV 223
>At2g39270 putative adenylate kinase
Length = 295
Score = 32.7 bits (73), Expect = 0.051
Identities = 22/66 (33%), Positives = 33/66 (49%), Gaps = 1/66 (1%)
Query: 7 KRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDG-WDDELDSYILNE 65
K R + + G PG GK T ++ L+ + HI GDLV+E+ G +L + +
Sbjct: 61 KGRNFHWVFLGCPGVGKGTYASRLSSLLGVPHIATGDLVREELSSSGLLSSQLKELVNHG 120
Query: 66 DLVCDE 71
LV DE
Sbjct: 121 KLVPDE 126
>At1g03000 putative peroxisome assembly factor-2
Length = 941
Score = 32.7 bits (73), Expect = 0.051
Identities = 23/73 (31%), Positives = 37/73 (50%), Gaps = 20/73 (27%)
Query: 8 RRKPNILVTGTPGTGKTTTSTALAEATQLNHINI--GDLV------KEKNLHDGWD---- 55
R++ +L+ G PGTGKT + A+A LN +++ +L+ EKN+ D ++
Sbjct: 689 RKRSGVLLYGPPGTGKTLLAKAVATECSLNFLSVKGPELINMYIGESEKNVRDIFEKARS 748
Query: 56 --------DELDS 60
DELDS
Sbjct: 749 ARPCVIFFDELDS 761
>At4g02470
Length = 371
Score = 32.3 bits (72), Expect = 0.066
Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 4/60 (6%)
Query: 3 KDSIKRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYI 62
K + + IL+ G PGTGKT + A+A N INI ++ W E + Y+
Sbjct: 97 KGQLTKPTKGILLFGPPGTGKTMLAKAVATEAGANFINI----SMSSITSKWFGEGEKYV 152
>At4g25280 UMP/CMP kinase like protein
Length = 227
Score = 31.6 bits (70), Expect = 0.11
Identities = 16/43 (37%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Query: 7 KRRKPNI-LVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEK 48
K + P I V G PG+GK T + E L H++ GDL++ +
Sbjct: 39 KEKAPFITFVLGGPGSGKGTQCEKIVETFGLQHLSAGDLLRRE 81
>At1g02890 hypothetical protein
Length = 1240
Score = 31.6 bits (70), Expect = 0.11
Identities = 16/39 (41%), Positives = 21/39 (53%)
Query: 3 KDSIKRRKPNILVTGTPGTGKTTTSTALAEATQLNHINI 41
K + + IL+ G PGTGKT + A+A N INI
Sbjct: 970 KGQLTKPTKGILLFGPPGTGKTMLAKAVATEAGANFINI 1008
>At5g53540 26S proteasome regulatory particle chain RPT6-like
protein
Length = 403
Score = 31.2 bits (69), Expect = 0.15
Identities = 17/42 (40%), Positives = 23/42 (54%), Gaps = 4/42 (9%)
Query: 13 ILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGW 54
+L+ G PGTGKT + A+A ++ IN VK NL W
Sbjct: 125 VLLYGPPGTGKTMLAKAIARESEAVFIN----VKVSNLMSKW 162
>At4g24710 protein binding protein like
Length = 467
Score = 31.2 bits (69), Expect = 0.15
Identities = 21/77 (27%), Positives = 38/77 (49%), Gaps = 10/77 (12%)
Query: 13 ILVTGTPGTGKTTTSTALAEATQLN------HINIGDLVKEKNLHDGWDDELDSYILNED 66
IL+ G PGTGKT+ ALA+ + H + + V +L W E +
Sbjct: 205 ILLHGPPGTGKTSLCKALAQKLSIRCNSRYPHCQLIE-VNAHSLFSKWFSESGKLVAK-- 261
Query: 67 LVCDELEDVMDEGGNIV 83
+ +++++++E GN+V
Sbjct: 262 -LFQKIQEMVEEDGNLV 277
>At1g77470 putative replication factor C
Length = 369
Score = 31.2 bits (69), Expect = 0.15
Identities = 18/46 (39%), Positives = 27/46 (58%), Gaps = 2/46 (4%)
Query: 7 KRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHD 52
+ + P++L+ G PGTGK TST LA A +L +++ E N D
Sbjct: 69 ENKLPHLLLYGPPGTGK--TSTILAVARKLYGPKYRNMILELNASD 112
>At1g21690 putative replication factor C subunit
Length = 339
Score = 31.2 bits (69), Expect = 0.15
Identities = 13/21 (61%), Positives = 17/21 (80%)
Query: 11 PNILVTGTPGTGKTTTSTALA 31
P++L G PGTGKTTT+ A+A
Sbjct: 43 PHMLFYGPPGTGKTTTALAIA 63
>At5g47010 UPF1
Length = 1254
Score = 30.8 bits (68), Expect = 0.19
Identities = 13/22 (59%), Positives = 16/22 (72%)
Query: 9 RKPNILVTGTPGTGKTTTSTAL 30
+KP L+ G PGTGKT TS A+
Sbjct: 502 QKPISLIQGPPGTGKTVTSAAI 523
>At4g29040 26S proteasome AAA-ATPase subunit RPT2a
Length = 443
Score = 30.4 bits (67), Expect = 0.25
Identities = 15/42 (35%), Positives = 25/42 (58%), Gaps = 1/42 (2%)
Query: 13 ILVTGTPGTGKTTTSTALAEATQLNHIN-IGDLVKEKNLHDG 53
+++ G PGTGKT + A+A +T + +G + +K L DG
Sbjct: 225 VILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDG 266
>At2g20140 26S proteasome subunit 4
Length = 443
Score = 30.4 bits (67), Expect = 0.25
Identities = 15/42 (35%), Positives = 25/42 (58%), Gaps = 1/42 (2%)
Query: 13 ILVTGTPGTGKTTTSTALAEATQLNHIN-IGDLVKEKNLHDG 53
+++ G PGTGKT + A+A +T + +G + +K L DG
Sbjct: 225 VILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDG 266
>At3g53230 CDC48 - like protein
Length = 815
Score = 30.0 bits (66), Expect = 0.33
Identities = 24/81 (29%), Positives = 35/81 (42%), Gaps = 14/81 (17%)
Query: 13 ILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYI------LNED 66
+L G PG GKT + A+A Q N I+I K L W E ++ + +
Sbjct: 518 VLFYGPPGCGKTLLAKAIANECQANFISI----KGPELLTMWFGESEANVREIFDKARQS 573
Query: 67 LVC----DELEDVMDEGGNIV 83
C DEL+ + + GN V
Sbjct: 574 APCVLFFDELDSIATQRGNSV 594
>At3g19740 ATPase, putative
Length = 439
Score = 30.0 bits (66), Expect = 0.33
Identities = 16/39 (41%), Positives = 22/39 (56%)
Query: 3 KDSIKRRKPNILVTGTPGTGKTTTSTALAEATQLNHINI 41
+ ++ R IL+ G PGTGKT + ALA N I+I
Sbjct: 178 RGNLLRPCKGILLFGPPGTGKTLLAKALATEAGANFISI 216
>At1g50140 hypothetical protein, 5' partial
Length = 601
Score = 30.0 bits (66), Expect = 0.33
Identities = 16/39 (41%), Positives = 22/39 (56%)
Query: 3 KDSIKRRKPNILVTGTPGTGKTTTSTALAEATQLNHINI 41
+ ++ R IL+ G PGTGKT + ALA N I+I
Sbjct: 340 RGNLLRPCKGILLFGPPGTGKTLLAKALATEAGANFISI 378
>At5g41120 unknown protein
Length = 684
Score = 29.6 bits (65), Expect = 0.43
Identities = 25/86 (29%), Positives = 38/86 (44%), Gaps = 7/86 (8%)
Query: 18 TPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDEL----DSYILNEDLVCDE-- 71
T GT T S+A +A L+ + + N G +L + Y E++ +E
Sbjct: 12 TDGTSPTFFSSASTKAYNLHFLYSNSTQRLTNPKFGIGGKLKVTVNPYSYTEEVRPEERK 71
Query: 72 -LEDVMDEGGNIVDYHGCDFFPERWF 96
L D + E G+ V+ G D P RWF
Sbjct: 72 SLTDFLTEAGDFVNSDGGDGGPPRWF 97
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.137 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,150,076
Number of Sequences: 26719
Number of extensions: 137028
Number of successful extensions: 678
Number of sequences better than 10.0: 123
Number of HSP's better than 10.0 without gapping: 94
Number of HSP's successfully gapped in prelim test: 29
Number of HSP's that attempted gapping in prelim test: 572
Number of HSP's gapped (non-prelim): 136
length of query: 127
length of database: 11,318,596
effective HSP length: 87
effective length of query: 40
effective length of database: 8,994,043
effective search space: 359761720
effective search space used: 359761720
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)
Medicago: description of AC124972.11