BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124972.10 - phase: 1 /pseudo
(135 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g59050 unknown protein 164 1e-41
At2g43020 putative amine oxidase 162 6e-41
At1g65840 polyamine oxidase, putative 133 2e-32
At4g16310 hypothetical protein 65 1e-11
At5g13700 polyamine oxidase 45 9e-06
At3g13682 hypothetical protein 44 3e-05
At3g10390 hypothetical protein 33 0.035
At1g62830 flavin-containing amine oxidase 33 0.059
At4g29720 unknown protein 31 0.23
At3g54010 pasticcino 1-A (PAS1-A) 27 3.3
At3g05210 putative nucleotide repair protein 27 3.3
At1g80540 hypothetical protein 27 3.3
At5g19010 MAP kinase -like protein 27 4.2
At1g59840 hypothetical protein 26 5.5
At1g59590 unknown protein 26 5.5
At5g62030 unknown protein (At5g62030) 26 7.2
At2g32920 putative protein disulfide isomerase 26 7.2
At1g14110 hypothetical protein 26 7.2
>At3g59050 unknown protein
Length = 488
Score = 164 bits (415), Expect = 1e-41
Identities = 80/114 (70%), Positives = 92/114 (80%)
Query: 12 LLTKLIKFEPKLLDWKEAAIADIRVGVENKIILHFKNVFWPNEDFLEVVAEISNGCSYFL 71
L + +I FEPKL WK+ AI D+ VG+ENKIIL+F NVFWPN +FL VVAE S GCSYFL
Sbjct: 281 LKSGMITFEPKLPQWKQEAINDLGVGIENKIILNFDNVFWPNVEFLGVVAETSYGCSYFL 340
Query: 72 NLHKAAGHSVLVYMPVGRQAKDIEKMSDEAAANFAFKQLKKILPDASSPVTIIV 125
NLHKA H VLVYMP G+ A+DIEK SDEAAANFAF QL+KILPDASSP+ +V
Sbjct: 341 NLHKATSHPVLVYMPAGQLARDIEKKSDEAAANFAFSQLQKILPDASSPINYLV 394
>At2g43020 putative amine oxidase
Length = 490
Score = 162 bits (409), Expect = 6e-41
Identities = 79/109 (72%), Positives = 89/109 (81%)
Query: 17 IKFEPKLLDWKEAAIADIRVGVENKIILHFKNVFWPNEDFLEVVAEISNGCSYFLNLHKA 76
IKFEPKL +WK+ AI D+ VG+ENKIILHF+ VFWP +FL VVAE S GCSYFLNLHKA
Sbjct: 285 IKFEPKLPEWKQEAINDLGVGIENKIILHFEKVFWPKVEFLGVVAETSYGCSYFLNLHKA 344
Query: 77 AGHSVLVYMPVGRQAKDIEKMSDEAAANFAFKQLKKILPDASSPVTIIV 125
GH VLVYMP G+ AKDIEKMSDEAAANFA QL++ILPDA PV +V
Sbjct: 345 TGHPVLVYMPAGQLAKDIEKMSDEAAANFAVLQLQRILPDALPPVQYLV 393
>At1g65840 polyamine oxidase, putative
Length = 497
Score = 133 bits (335), Expect = 2e-32
Identities = 65/114 (57%), Positives = 77/114 (67%)
Query: 12 LLTKLIKFEPKLLDWKEAAIADIRVGVENKIILHFKNVFWPNEDFLEVVAEISNGCSYFL 71
L LI+FEP+L WK +AI+ + VG ENKI L F FWPN +FL +VA S C YFL
Sbjct: 282 LKANLIQFEPELPQWKTSAISGLGVGNENKIALRFDRAFWPNVEFLGMVAPTSYACGYFL 341
Query: 72 NLHKAAGHSVLVYMPVGRQAKDIEKMSDEAAANFAFKQLKKILPDASSPVTIIV 125
NLHKA GH VLVYM G A+D+EK+SDEA ANF QLKK+ PDA P +V
Sbjct: 342 NLHKATGHPVLVYMAAGNLAQDLEKLSDEATANFVMLQLKKMFPDAPDPAQYLV 395
>At4g16310 hypothetical protein
Length = 1265
Score = 65.1 bits (157), Expect = 1e-11
Identities = 38/106 (35%), Positives = 53/106 (49%), Gaps = 4/106 (3%)
Query: 12 LLTKLIKFEPKLLDWKEAAIADIRVGVENKIILHFKNVFWPNE-DFLEVVAE---ISNGC 67
L + IKF P L DWK A+I + GV NK++L F VFW + D+ AE + C
Sbjct: 899 LKAETIKFSPPLPDWKYASIKQLGFGVLNKVVLEFPTVFWDDSVDYFGATAEETDLRGEC 958
Query: 68 SYFLNLHKAAGHSVLVYMPVGRQAKDIEKMSDEAAANFAFKQLKKI 113
F N+ K G VL+ + VG+ A + S N A L+K+
Sbjct: 959 FMFWNVKKTVGAPVLIALVVGKAAFEYTNKSKSEHVNHAMMVLRKL 1004
>At5g13700 polyamine oxidase
Length = 472
Score = 45.4 bits (106), Expect = 9e-06
Identities = 31/104 (29%), Positives = 47/104 (44%), Gaps = 4/104 (3%)
Query: 12 LLTKLIKFEPKLLDWKEAAIADIRVGVENKIILHFKNVFW---PNEDFLEVVAEISNGCS 68
L + L+ F+P L WK AI V V KI L F FW P ++F E +
Sbjct: 247 LQSDLLSFQPLLPRWKTEAIQKCDVMVYTKIFLKFPQCFWPCGPGQEFFIYAHEQRGYFT 306
Query: 69 YFLNLHKA-AGHSVLVYMPVGRQAKDIEKMSDEAAANFAFKQLK 111
++ ++ A G ++LV Q+K +E SD+ A L+
Sbjct: 307 FWQHMENAYPGSNILVVTLTNEQSKRVEAQSDQETMKEAMSVLR 350
>At3g13682 hypothetical protein
Length = 746
Score = 43.9 bits (102), Expect = 3e-05
Identities = 35/106 (33%), Positives = 52/106 (49%), Gaps = 4/106 (3%)
Query: 12 LLTKLIKFEPKLLDWKEAAIADIRVGVENKIILHFKNVFWPNE-DFLEVVAEIS-NGCSY 69
L + IKFEP+L K+AAI + G+ NK+ + F +VFW +E D + E S N +
Sbjct: 407 LKKRSIKFEPELPRRKQAAIDRLGFGLLNKVAMLFPSVFWGDELDTFGCLNESSINRGEF 466
Query: 70 FL--NLHKAAGHSVLVYMPVGRQAKDIEKMSDEAAANFAFKQLKKI 113
FL H +G LV + G A+ E + K+L+ I
Sbjct: 467 FLFYAYHTVSGGPALVALVAGEAAQRFECTEPSVLLHRVLKKLRGI 512
>At3g10390 hypothetical protein
Length = 789
Score = 33.5 bits (75), Expect = 0.035
Identities = 24/87 (27%), Positives = 39/87 (44%), Gaps = 8/87 (9%)
Query: 17 IKFEPKLLDWKEAAIADIRVGVENKIILHFKNVFWPNEDFLEVVAEISNGCSY------F 70
IKF P+L K I + G+ NK+ + F VFW + L+ ++ +Y F
Sbjct: 437 IKFVPELPQRKLDCIKRLGFGLLNKVAMLFPYVFWSTD--LDTFGHLTEDPNYRGEFFLF 494
Query: 71 LNLHKAAGHSVLVYMPVGRQAKDIEKM 97
+ AG ++L+ + G A E M
Sbjct: 495 YSYAPVAGGALLIALVAGEAAHKFETM 521
>At1g62830 flavin-containing amine oxidase
Length = 844
Score = 32.7 bits (73), Expect = 0.059
Identities = 31/113 (27%), Positives = 52/113 (45%), Gaps = 8/113 (7%)
Query: 17 IKFEPKLLDWKEAAIADIRVGVENKIILHFKNVFWPNE--DFLEVVAEISNGCSYFL--N 72
I+F P+L K+ AI + G+ NK+ + F FW E F + + S +FL +
Sbjct: 521 IEFYPELPHKKKEAIQRLGFGLLNKVAMLFPCNFWGEEIDTFGRLTEDPSTRGEFFLFYS 580
Query: 73 LHKAAGHSVLVYMPVGRQAKDIEKMSDEAAANFAFKQLKKILPDASSPVTIIV 125
+G +LV + G A+ E +S + K++ +IL P I+V
Sbjct: 581 YSSVSGGPLLVALVAGDAAERFETLSPTDSV----KRVLQILRGIYHPKGIVV 629
>At4g29720 unknown protein
Length = 533
Score = 30.8 bits (68), Expect = 0.23
Identities = 30/125 (24%), Positives = 56/125 (44%), Gaps = 19/125 (15%)
Query: 19 FEPKLLDWKEAAIADIRVGVENKIILHFKNVFWPNEDFLEVVAEISNGCSYFLN----LH 74
F P L D+K AI + GV NK+ + +P+ L++V + + F+ +
Sbjct: 307 FSPPLPDFKSDAIRRLGYGVVNKLFVEMSQRKFPS---LQLVFDREDSEFRFVKIPWWMR 363
Query: 75 KAA-------GHSVLVYMPVGRQAKDIEKMSDEAAANFAFKQL-----KKILPDASSPVT 122
+ A VL+ G++A ++EK++DE + + K++ D + P+T
Sbjct: 364 RTATITPIHSNSKVLLSWFAGKEALELEKLTDEEIKDAVMTTISCLTGKEVKNDTAKPLT 423
Query: 123 IIVLN 127
LN
Sbjct: 424 NGSLN 428
>At3g54010 pasticcino 1-A (PAS1-A)
Length = 635
Score = 26.9 bits (58), Expect = 3.3
Identities = 19/48 (39%), Positives = 27/48 (55%), Gaps = 1/48 (2%)
Query: 17 IKFEPKLLDWKEAAIADIRVGVENKIILHFKNVFWPNEDFLEVVAEIS 64
+ FE +LLD+ +A IA +GV KI+ + P E + EV A IS
Sbjct: 137 LHFEIELLDFSKAKIASDDLGVIKKILNEGEGWESPREPY-EVKARIS 183
>At3g05210 putative nucleotide repair protein
Length = 410
Score = 26.9 bits (58), Expect = 3.3
Identities = 13/56 (23%), Positives = 24/56 (42%)
Query: 40 NKIILHFKNVFWPNEDFLEVVAEISNGCSYFLNLHKAAGHSVLVYMPVGRQAKDIE 95
N ++ H +NV W D + N C+ +L+L H +Y + K+ +
Sbjct: 135 NPLLKHIRNVKWVFSDIIPDYVLGQNSCALYLSLRYHLLHPDYLYFRIRELQKNFK 190
>At1g80540 hypothetical protein
Length = 481
Score = 26.9 bits (58), Expect = 3.3
Identities = 23/103 (22%), Positives = 42/103 (40%), Gaps = 8/103 (7%)
Query: 38 VENKIILHFKNVFWPNEDFLEVVAEISNGCSYFLNLHKAAGHSVLVYMPVGRQAKDIEKM 97
V N+ I + P L V+A + ++ L G VLVY+ V +++
Sbjct: 208 VANQTIRYLTGALNPVRYVLNVIAGVMLAVAFLGLLFSFCGLRVLVYLVVADTCMAMDQW 267
Query: 98 SDEAAANFAFKQLKKILP--------DASSPVTIIVLNLFNSF 132
+ AA+ A QL L D + +T +++ N++
Sbjct: 268 VHDPAADSALSQLLPCLDPKTIGETLDITKTMTATAVDMTNAY 310
>At5g19010 MAP kinase -like protein
Length = 567
Score = 26.6 bits (57), Expect = 4.2
Identities = 12/24 (50%), Positives = 16/24 (66%)
Query: 11 RLLTKLIKFEPKLLDWKEAAIADI 34
RLL K++ FEPK E A+AD+
Sbjct: 291 RLLEKMLSFEPKDRPTAEEALADV 314
>At1g59840 hypothetical protein
Length = 305
Score = 26.2 bits (56), Expect = 5.5
Identities = 15/46 (32%), Positives = 22/46 (47%), Gaps = 3/46 (6%)
Query: 61 AEISNGC---SYFLNLHKAAGHSVLVYMPVGRQAKDIEKMSDEAAA 103
A +N C SY NL G S L ++P QA ++ + D+ A
Sbjct: 216 ARKNNDCLSESYLANLSLYPGRSELPFLPANTQAVILQPLGDKGIA 261
>At1g59590 unknown protein
Length = 211
Score = 26.2 bits (56), Expect = 5.5
Identities = 15/45 (33%), Positives = 27/45 (59%), Gaps = 1/45 (2%)
Query: 90 QAKDIEKMSDEAAANFAFKQLKKIL-PDASSPVTIIVLNLFNSFF 133
+ K+ E +++ + + LKKI PD PVT+I++ +F +FF
Sbjct: 104 EIKNTEPETEQKQSCVINENLKKIARPDHFLPVTVILVLVFLAFF 148
>At5g62030 unknown protein (At5g62030)
Length = 453
Score = 25.8 bits (55), Expect = 7.2
Identities = 16/48 (33%), Positives = 24/48 (49%), Gaps = 3/48 (6%)
Query: 21 PKL-LDWKEAAIADIRVGVENKIILHFKNVFWPNEDFLEVVAEISNGC 67
P+L +DW EA + + E +I L F +W N+ V S+GC
Sbjct: 343 PRLSIDWGEAFLKPLLTTFEAEIALGFIRGWWENDSSSRV--NSSSGC 388
>At2g32920 putative protein disulfide isomerase
Length = 440
Score = 25.8 bits (55), Expect = 7.2
Identities = 14/38 (36%), Positives = 25/38 (64%), Gaps = 2/38 (5%)
Query: 80 SVLVYMPVGRQAKDIEKMSD-EAAANFAFKQLKKILPD 116
++ V++P G+ D + D ++ ANFA+KQ+K +L D
Sbjct: 105 TIKVFVP-GKAPIDYQGARDAKSIANFAYKQIKGLLSD 141
>At1g14110 hypothetical protein
Length = 435
Score = 25.8 bits (55), Expect = 7.2
Identities = 21/83 (25%), Positives = 33/83 (39%), Gaps = 18/83 (21%)
Query: 14 TKLIKFEPKLLDWKEAAIADIRVGVENKIILHFKNVFWPNEDFLEVVAEISNGCSYFLNL 73
TKLIK P+ KE + + + H N W D L+ + FL
Sbjct: 267 TKLIKLFPQ----KETVFHHLA-----RYLFHPTNQVWEYSDHLK---------NMFLEQ 308
Query: 74 HKAAGHSVLVYMPVGRQAKDIEK 96
+ G ++ VY P G + + +K
Sbjct: 309 ASSTGETIEVYQPSGEKIQQTDK 331
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.324 0.138 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,752,017
Number of Sequences: 26719
Number of extensions: 101012
Number of successful extensions: 330
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 315
Number of HSP's gapped (non-prelim): 18
length of query: 135
length of database: 11,318,596
effective HSP length: 88
effective length of query: 47
effective length of database: 8,967,324
effective search space: 421464228
effective search space used: 421464228
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 54 (25.4 bits)
Medicago: description of AC124972.10