BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124967.4 - phase: 0 /pseudo
(335 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g07410 pectin methyl-esterase-like protein 244 6e-65
At1g69940 pectinesterase like protein 244 6e-65
At5g61680 pectin methyl-esterase - like protein 243 8e-65
At5g07420 pectin methyl-esterase-like protein 220 1e-57
At5g07430 pectin methyl-esterase-like protein 214 7e-56
At5g19730 pectin methylesterase-like protein 157 1e-38
At5g47500 pectin methylesterase-like 155 4e-38
At2g36710 putative pectinesterase 152 2e-37
At1g05310 putative pectin methylesterase 151 6e-37
At3g29090 pectinesterase like protein 149 2e-36
At2g36700 putative pectinesterase 148 5e-36
At5g55590 putative protein 144 7e-35
At2g21610 putative pectinesterase 124 7e-29
At3g06830 pectin methylesterase like protein 120 8e-28
At2g47030 putative pectinesterase 120 1e-27
At5g49180 pectin methylesterase 117 1e-26
At3g17060 pectinesterase, putative 115 3e-26
At4g15980 pectinesterase like protein 111 6e-25
At5g27870 pectin methyl-esterase - like protein 110 1e-24
At4g02300 putative pectinesterase 110 1e-24
>At5g07410 pectin methyl-esterase-like protein
Length = 361
Score = 244 bits (622), Expect = 6e-65
Identities = 132/317 (41%), Positives = 189/317 (58%), Gaps = 32/317 (10%)
Query: 40 LEEHLTAAETTVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDR 99
L+ L AAE R++ V G G+F T+TDA+KS+P+GN +RV++ + GEYREK+T+DR
Sbjct: 53 LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDR 111
Query: 100 SKRFVTFYGERNGKDNDMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMP 159
+K F+T G+ N MP+ITYD TA +YGT+DSA++ + +DYF+AVN+ N++P P
Sbjct: 112 NKPFITLMGQPNA-----MPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAP 166
Query: 160 DENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKS 219
D + G QAL+MRISG+ AAFYNCKF GFQDT+CDD G HFFKDC+++GT+DFIFG+G S
Sbjct: 167 DGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTS 226
Query: 220 IYLRTTIESVAKGFE--CHHS-------------------TR*GKNVGRHWIHFCSLQHN 258
+YL T + V G H+ T G +GR W+ + +
Sbjct: 227 MYLGTQLHVVGDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYA 286
Query: 259 W-KWSQEHLPWSWLEEEP*GCICLYLYGLCRQFQRMVPPWVQ*AVRLNYKRILSDEEAKH 317
+ + + P W E + YG + P A R+ + + + D+EA
Sbjct: 287 YTEMTSVVNPTGWQENKTPAHDKTVFYGE----YKCSGPGSHKAKRVPFTQDIDDKEANC 342
Query: 318 FLSMAYIHGEQWVRPPP 334
FLS+ YI G +W+ PPP
Sbjct: 343 FLSLGYIQGSKWLLPPP 359
>At1g69940 pectinesterase like protein
Length = 361
Score = 244 bits (622), Expect = 6e-65
Identities = 131/317 (41%), Positives = 189/317 (59%), Gaps = 32/317 (10%)
Query: 40 LEEHLTAAETTVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDR 99
L+ L AAE R++ V G G+F T+TDA+KS+P+GN +RV++ + GEY+EK+T+DR
Sbjct: 53 LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDR 111
Query: 100 SKRFVTFYGERNGKDNDMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMP 159
+K F+T G+ N MP+ITYD TA +YGT+DSA++ + +DYF+AVN+ N++P P
Sbjct: 112 NKPFITLMGQPNA-----MPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAP 166
Query: 160 DENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKS 219
D + G QAL+MRISG+ AAFYNCKF GFQDT+CDD G HFFKDC+++GT+DFIFG+G S
Sbjct: 167 DGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTS 226
Query: 220 IYLRTTIESVAKGFE--CHHS-------------------TR*GKNVGRHWIHFCSLQHN 258
+YL T + V G H+ T G +GR W+ + +
Sbjct: 227 MYLGTQLHVVGDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYA 286
Query: 259 W-KWSQEHLPWSWLEEEP*GCICLYLYGLCRQFQRMVPPWVQ*AVRLNYKRILSDEEAKH 317
+ + + P W E + YG + P A R+ + + + D+EA
Sbjct: 287 YTEMTSVVNPTGWQENKTPAHDKTVFYGE----YKCSGPGSHKAKRVPFTQDIDDKEANR 342
Query: 318 FLSMAYIHGEQWVRPPP 334
FLS+ YI G +W+ PPP
Sbjct: 343 FLSLGYIQGSKWLLPPP 359
>At5g61680 pectin methyl-esterase - like protein
Length = 338
Score = 243 bits (621), Expect = 8e-65
Identities = 130/301 (43%), Positives = 179/301 (59%), Gaps = 31/301 (10%)
Query: 56 VRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGKDN 115
V+++G G F T+T+A+ S+ +GN RRV++ IG G Y+EK+T+DRSK F+T YG N
Sbjct: 45 VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGHPNA--- 101
Query: 116 DMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRISG 175
MP++T+D TA +YGT+DSAT+ V +DYF+AVN+ NS+PMPD G QAL+MRISG
Sbjct: 102 --MPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISG 159
Query: 176 DKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTTIESVA----- 230
+KAAFYNCKF G+QDT+CDD G HFFKDC+I+GT+DFIFG+G+S+YL T + V
Sbjct: 160 NKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGDGIRV 219
Query: 231 ------------KGFECHH----STR*GKNVGRHWIHFCSLQHNWK-WSQEHLPWSWLEE 273
G+ H T G +GR W+ + + + S P W E
Sbjct: 220 ITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQEN 279
Query: 274 EP*GCICLYLYGLCRQFQRMVPPWVQ*AVRLNYKRILSDEEAKHFLSMAYIHGEQWVRPP 333
G YG + R+ Y + + D EAK+F+S+ YI G W+ PP
Sbjct: 280 REAGRDKTVFYGE----YKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPP 335
Query: 334 P 334
P
Sbjct: 336 P 336
>At5g07420 pectin methyl-esterase-like protein
Length = 361
Score = 220 bits (560), Expect = 1e-57
Identities = 128/319 (40%), Positives = 180/319 (56%), Gaps = 36/319 (11%)
Query: 40 LEEHLTAAETTVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDR 99
L+ L AAE + RV+ V ++G GDF T+ A+KSIP NK RV++ + G Y EK+TVD
Sbjct: 53 LDPELEAAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDV 112
Query: 100 SKRFVTFYGERNGKDNDMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMP 159
+ +VT G+ + N +TY TA +YGT++SAT+ V A F+A N+ +N+SPMP
Sbjct: 113 GRPYVTLLGKPGAETN-----LTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMP 167
Query: 160 DENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKS 219
+ G QALAMRI+GDKAAFYNC+F GFQDTLCDD G HFFK+C+I+GTYDFIFG G S
Sbjct: 168 KPGTQG-QALAMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGAS 226
Query: 220 IYLRTTIESVAKGFE---CHH------------------STR*GKNVGRHWIHFCSLQHN 258
+YL T + +V G H+ G +GR W+ + ++
Sbjct: 227 LYLTTQLHAVGDGLRVIAAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYS 286
Query: 259 W-KWSQEHLPWSWLEEEP*GCICLYLYG--LCRQFQRMVPPWVQ*AVRLNYKRILSDEEA 315
+ + S P W E YG +C P A R+ + + + ++EA
Sbjct: 287 YTEMSSVVNPSGWQENRVRAHDKTVFYGEYMC------TGPGSHKAKRVAHTQDIDNKEA 340
Query: 316 KHFLSMAYIHGEQWVRPPP 334
FL++ YI G +W+ PPP
Sbjct: 341 SQFLTLGYIKGSKWLLPPP 359
>At5g07430 pectin methyl-esterase-like protein
Length = 361
Score = 214 bits (544), Expect = 7e-56
Identities = 122/318 (38%), Positives = 176/318 (54%), Gaps = 32/318 (10%)
Query: 40 LEEHLTAAETTVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDR 99
L+ L AAE +++ V + G +F T+ +A+KSIP+GNK RV++ + G Y EK+T+D
Sbjct: 53 LDPALEAAEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDI 112
Query: 100 SKRFVTFYGERNGKDNDMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMP 159
++ F+T G+ + ++TY TA +YGT++SAT+ V A+YF A ++ N++PMP
Sbjct: 113 ARPFITLLGQPGAET-----VLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMP 167
Query: 160 DENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKS 219
S G QALAMRI+ DKAAFY+C+F GFQDTLCDD G HFFKDC+I+GTYDFIFG G S
Sbjct: 168 KPGSQG-QALAMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGAS 226
Query: 220 IYLRTTIESVAKGFECHHS---------------------TR*GKNVGRHWIHFCSLQHN 258
+YL T + +V G + T G +GR W+ + +
Sbjct: 227 LYLNTQLHAVGDGLRVITAQGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYA 286
Query: 259 W-KWSQEHLPWSWLEEEP*GCICLYLYGLCRQFQRMVPPWVQ*AVRLNYKRILSDEEAKH 317
+ + + P W E G YG + F P R+ Y + + E
Sbjct: 287 FTEMTSVVNPSGWRENLNRGYDKTVFYGEYKCFG----PGSHLEKRVPYTQDIDKNEVTP 342
Query: 318 FLSMAYIHGEQWVRPPPK 335
FL++ YI G W+ PPPK
Sbjct: 343 FLTLGYIKGSTWLLPPPK 360
>At5g19730 pectin methylesterase-like protein
Length = 383
Score = 157 bits (396), Expect = 1e-38
Identities = 100/302 (33%), Positives = 153/302 (50%), Gaps = 35/302 (11%)
Query: 57 RKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGK--- 113
+K GDFT + DA+ S+P N RVV+ + G Y+EK+++ K F+T GE K
Sbjct: 89 KKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTV 148
Query: 114 ---DNDMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALA 170
D P D+ GT +SA+ AV++ +FVA N+ F N++P+P +VG QA+A
Sbjct: 149 EWGDTAQTP----DSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVA 204
Query: 171 MRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTTIESVA 230
+R+S D AAF+ C+ +G QDTL D G+H++KDC+I+G+ DFIFGN S+Y + ++A
Sbjct: 205 LRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIA 264
Query: 231 -----------------KGF---ECHHSTR*GKNVGRHWIHFCSLQHNWKWSQE-HLPWS 269
GF +C + +GR W F + + + LP
Sbjct: 265 DKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRG 324
Query: 270 WLEEEP*GCICLYLYGLCRQFQRMVPPWVQ*AVRLNYKRILSDEEAKHFLSMAYIHGEQW 329
W YG + R+ + R L+DEEAK FLS+ +I G +W
Sbjct: 325 WYNWGDPSREMTVFYGQ----YKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEW 380
Query: 330 VR 331
++
Sbjct: 381 IK 382
>At5g47500 pectin methylesterase-like
Length = 362
Score = 155 bits (391), Expect = 4e-38
Identities = 81/188 (43%), Positives = 113/188 (60%), Gaps = 10/188 (5%)
Query: 52 RVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERN 111
+V+ V +G F +V DAV SIP N + + + I G YREK+ V +K ++TF
Sbjct: 59 KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITF----K 114
Query: 112 GKDNDMMPIITYD------ATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVG 165
G D+ I +D A + T +A+V V A+YF A N++F N++P P G
Sbjct: 115 GAGRDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQG 174
Query: 166 GQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTT 225
QA+A RISGDKA F C F G QDTLCDD G+H+FK+C+I+G+ DFIFGNG+S+Y
Sbjct: 175 WQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCE 234
Query: 226 IESVAKGF 233
+ S+A F
Sbjct: 235 LHSIASRF 242
>At2g36710 putative pectinesterase
Length = 407
Score = 152 bits (384), Expect = 2e-37
Identities = 86/206 (41%), Positives = 119/206 (57%), Gaps = 9/206 (4%)
Query: 31 FNRHREITLLEE---HLTAAETTVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIG 87
++ H I E+ L T V+ V G G+F+ V A+ +P + + ++ +
Sbjct: 65 YHHHEPIKCCEKWTSRLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVN 124
Query: 88 MGEYREKITVDRSKRFVTFYGERNGKDNDMMPIITYDATALRYG-TLDSATVAVDADYFV 146
G YREK+TV+ +K + G G N I ++ TA G T DS + V A F
Sbjct: 125 SGSYREKVTVNENKTNLVIQGR--GYQNTS---IEWNDTAKSAGNTADSFSFVVFAANFT 179
Query: 147 AVNVAFVNSSPMPDENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFI 206
A N++F N++P PD QA+A+RI GD+AAFY C F G QDTL DD G+HFFK+CFI
Sbjct: 180 AYNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFI 239
Query: 207 QGTYDFIFGNGKSIYLRTTIESVAKG 232
QG+ DFIFGNG+S+Y TI S+AKG
Sbjct: 240 QGSIDFIFGNGRSLYQDCTINSIAKG 265
>At1g05310 putative pectin methylesterase
Length = 393
Score = 151 bits (381), Expect = 6e-37
Identities = 80/181 (44%), Positives = 112/181 (61%), Gaps = 4/181 (2%)
Query: 50 TVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGE 109
T + V K+G +FTTV AV ++ + ++RR V+WI G Y EK+ + ++K +T G+
Sbjct: 87 TTSYLCVDKNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQ 146
Query: 110 RNGKDNDMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQAL 169
D+ I D GT ATV V FVA N++F+N +P+P VG QA+
Sbjct: 147 ----GFDITAIAWNDTAYSANGTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAV 202
Query: 170 AMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTTIESV 229
A+RI+GD++AF C F G QDTL DD G+H+FKDC+IQG+ DFIFGN KS+Y I S+
Sbjct: 203 AIRIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISM 262
Query: 230 A 230
A
Sbjct: 263 A 263
>At3g29090 pectinesterase like protein
Length = 317
Score = 149 bits (376), Expect = 2e-36
Identities = 99/315 (31%), Positives = 157/315 (49%), Gaps = 53/315 (16%)
Query: 50 TVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGE 109
T R+VRV +DG+GD+ +V DA+ S+P GN R V+ + G YR+ + V + K F+TF G
Sbjct: 3 TTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFAGI 62
Query: 110 RNGKDNDMMPIITYDATALRY-----------GTLDSATVAVDADYFVAVNVAFVNSSPM 158
+ + ++T++ TA + GT +V V+ + F+A N+ F NS+P
Sbjct: 63 -----SPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAP- 116
Query: 159 PDENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGK 218
E S GQA+A+R++ D+ AFYNC+F+G+QDTL +GK + KDC+I+G+ DFIFGN
Sbjct: 117 --EGS--GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNST 172
Query: 219 SIYLRTTIESVAKGFECHHSTR*GKNVGRHWIHFCSLQHNWKWSQEHL--PW-------- 268
++ I ++GF S + + + C + N + +L PW
Sbjct: 173 ALLEHCHIHCKSQGFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPWGPFGRVVL 232
Query: 269 ---------------SWLEEEP*GCICLYLYGLCRQFQRMVPPWVQ*AVRLNYKRILSDE 313
+W E C Y Y R P + R+ + R L D+
Sbjct: 233 AYTYMDACIRNVGWHNWGNAENERSACFYEY-------RCFGPGSCSSERVPWSRELMDD 285
Query: 314 EAKHFLSMAYIHGEQ 328
EA HF+ +++ EQ
Sbjct: 286 EAGHFVHHSFVDPEQ 300
>At2g36700 putative pectinesterase
Length = 333
Score = 148 bits (373), Expect = 5e-36
Identities = 99/314 (31%), Positives = 154/314 (48%), Gaps = 33/314 (10%)
Query: 42 EHLTAAETT--VRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDR 99
+HL+ T V++V +G G F V DA+ + ++ + ++ I G YRE+ V
Sbjct: 28 DHLSKFPTKGFTMVLKVSLNGCGRFKRVQDAIDASIGSSQSKTLILIDFGIYRERFIVHE 87
Query: 100 SKRFVTFYGERNGKDNDMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMP 159
+K + G + + I + TA GT S +VAV + F A N++F N++P P
Sbjct: 88 NKNNLVVQGMGYSRTS----IEWNNTTASSNGTFSSFSVAVFGEKFTAYNISFKNTAPAP 143
Query: 160 DENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKS 219
+ +V QA+A+++ GDKAAFY C F G QDTL D G+HFFK CFI+G+ DFIFGNG+S
Sbjct: 144 NPGAVDAQAVALKVVGDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRS 203
Query: 220 IYLRTTIESVAKGFECHHSTR*GKN----------------------VGRHWIHFCSLQH 257
+Y T+ S+AK T GK+ +GR W + +
Sbjct: 204 LYEDCTLHSIAKENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIF 263
Query: 258 NWKWSQEHLPW-SWLEEEP*GCICLYLYGLCRQFQRMVPPWVQ*AVRLNYKRILSDEEAK 316
+ + + W + YG R P + R+ Y ++LSD EA
Sbjct: 264 SKTYMSRVVSLDGWNDMGDPKTQRTVYYG----EHRCYGPGANHSKRVTYAKLLSDVEAA 319
Query: 317 HFLSMAYIHGEQWV 330
F ++++I GE+W+
Sbjct: 320 PFTNISFIDGEEWL 333
>At5g55590 putative protein
Length = 380
Score = 144 bits (363), Expect = 7e-35
Identities = 101/313 (32%), Positives = 155/313 (49%), Gaps = 46/313 (14%)
Query: 52 RVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERN 111
RV+ V K+G GD TV AV +P N +RV ++I G YREK+ V +SK +++F G +
Sbjct: 80 RVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNES 139
Query: 112 GKDN------DMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVG 165
+ D + D L GT +A+V++++D+F A + F N+ + + G
Sbjct: 140 YAGDTVISWSDKASDLGCDGKEL--GTYRTASVSIESDFFCATAITFENTV-VAEAGEQG 196
Query: 166 GQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTT 225
QA+A+RI GDKA FY + +G QDTL DD G H+F C+IQG DFIFGN KS+Y
Sbjct: 197 RQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCD 256
Query: 226 IESVAKGF---ECHHSTR*GKNVGRHWIHFCSLQHNWKWSQEHLPWSWLEEEP*GCICLY 282
I S AK + HH ++ G +++ C + Q +L +W G
Sbjct: 257 IHSTAKRYGAIAAHHRDSETEDTGFSFVN-CDISGT---GQIYLGRAW------GNYSRT 306
Query: 283 LYGLC------------------RQFQRMVPPW------VQ*AVRLNYKRILSDEEAKHF 318
+Y C RQ + M + + R+ + + L+ +E K F
Sbjct: 307 VYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPF 366
Query: 319 LSMAYIHGEQWVR 331
L +I+G+QW+R
Sbjct: 367 LGREFIYGDQWLR 379
>At2g21610 putative pectinesterase
Length = 352
Score = 124 bits (311), Expect = 7e-29
Identities = 90/309 (29%), Positives = 143/309 (46%), Gaps = 41/309 (13%)
Query: 49 TTVRVVRVRKDGTGDFTTVTDAVKSIPSG--NKRRVVVWIGMGEYREKITVDRSKRFVTF 106
+T ++RV + G GDF+ + +A++SIP N + +W+ G YREK+ + K ++T
Sbjct: 46 STAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITL 105
Query: 107 YGERNGKDNDMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGG 166
G + +I D + L+S T+ + A FV + N G
Sbjct: 106 SGTQASNTF----LIWSDGEDI----LESPTLTIFASDFVCRFLTIQNKF------GTAG 151
Query: 167 QALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTTI 226
+A+A+R++ DKAAFY C +QDTL DD G H+FK+C+I+G DFI G+ S+Y R +
Sbjct: 152 RAVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHL 211
Query: 227 ESVA-----------------KGFE---CHHSTR*GKNVGRHWIHFCSLQHNWK-WSQEH 265
S++ GF C + +GR W + + + +S
Sbjct: 212 HSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVV 271
Query: 266 LPWSWLEEEP*GCICLYLYGLCRQFQRMVPPWVQ*AVRLNYKRILSDEEAKHFLSMAYIH 325
P W + YG + + P R+ + + LSDEEA FLS +I
Sbjct: 272 APQGWNQWGDSTKENTVYYGEYKCY----GPGADREQRVEWSKQLSDEEATVFLSKDFIG 327
Query: 326 GEQWVRPPP 334
G+ W+RP P
Sbjct: 328 GKDWLRPAP 336
>At3g06830 pectin methylesterase like protein
Length = 568
Score = 120 bits (302), Expect = 8e-28
Identities = 70/197 (35%), Positives = 106/197 (53%), Gaps = 20/197 (10%)
Query: 56 VRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGKDN 115
V +DGTG F T+TDA+ ++P GNK ++ I G Y+EK+TV + VTF G+ K
Sbjct: 260 VAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYKEKVTVTKKMPHVTFIGDGPNK-- 317
Query: 116 DMMPIITYDATALRYG-----TLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALA 170
T +L +G T +AT+ ++ D+F A N+ N++ GGQA+A
Sbjct: 318 ------TLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENTA-----GPEGGQAVA 366
Query: 171 MRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIY--LRTTIES 228
+R+S D A F++C+ G QDTL + F++DC + GT DFIFG+ K I + +
Sbjct: 367 LRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCILQNCKIVVRK 426
Query: 229 VAKGFECHHSTR*GKNV 245
KG C + + NV
Sbjct: 427 PNKGQTCMVTAQGRSNV 443
>At2g47030 putative pectinesterase
Length = 588
Score = 120 bits (300), Expect = 1e-27
Identities = 67/187 (35%), Positives = 112/187 (59%), Gaps = 14/187 (7%)
Query: 56 VRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGKDN 115
V KDG+G F TV AV + P N+ R +++I G YRE++ + + K + +G D
Sbjct: 279 VAKDGSGQFKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFG-----DG 333
Query: 116 DMMPIITYD-ATALRYGTLDS--ATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMR 172
+I+Y+ + AL GT S ATV V+++ F+A + F N++ +G QA A+R
Sbjct: 334 ARKTVISYNRSVALSRGTTTSLSATVQVESEGFMAKWMGFKNTA-----GPMGHQAAAIR 388
Query: 173 ISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTTIESVAKG 232
++GD+A +NC+F G+QDTL + G+ F+++C + GT DFIFG ++ ++ T+ V KG
Sbjct: 389 VNGDRAVIFNCRFDGYQDTLYVNNGRQFYRNCVVSGTVDFIFGKSATV-IQNTLIVVRKG 447
Query: 233 FECHHST 239
+ ++T
Sbjct: 448 SKGQYNT 454
>At5g49180 pectin methylesterase
Length = 571
Score = 117 bits (292), Expect = 1e-26
Identities = 63/164 (38%), Positives = 92/164 (55%), Gaps = 8/164 (4%)
Query: 54 VRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGK 113
V V DG+G + T+ +A+ ++P N++ V++I G Y EK+ V + VTF G+ K
Sbjct: 260 VVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTK 319
Query: 114 DNDMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRI 173
+ Y Y T ATVA++ D F A N+ F N++ G QA+A+R+
Sbjct: 320 TKITGSLNYYIGKVKTYLT---ATVAINGDNFTAKNIGFENTA-----GPEGHQAVALRV 371
Query: 174 SGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNG 217
S D A FYNC+ G+QDTL + FF+DC + GT DFIFG+G
Sbjct: 372 SADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDG 415
>At3g17060 pectinesterase, putative
Length = 344
Score = 115 bits (288), Expect = 3e-26
Identities = 58/178 (32%), Positives = 100/178 (55%), Gaps = 7/178 (3%)
Query: 44 LTAAETTVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRF 103
LT T R + V +G GD+T+V A+ ++P GN ++V + G Y+E++ + +K F
Sbjct: 36 LTEKIATNRSIIVDIEGKGDYTSVQKAIDAVPVGNSNWIIVHVRKGIYKERVHIPENKPF 95
Query: 104 VTFYGERNGKDNDMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENS 163
+ G GK +++ + SAT V+A++FVA ++ N +P+ +
Sbjct: 96 IFMRGNGKGKT-------VIESSQSSVDNVASATFKVEANHFVAFGISIRNDAPVGMAFT 148
Query: 164 VGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIY 221
Q++A ++ DK AFY+C F +TL D+ G+H++ +C+IQG+ DFIFG SI+
Sbjct: 149 SENQSVAAFVAADKVAFYHCAFYSLHNTLFDNKGRHYYHECYIQGSIDFIFGRATSIF 206
>At4g15980 pectinesterase like protein
Length = 701
Score = 111 bits (277), Expect = 6e-25
Identities = 61/170 (35%), Positives = 97/170 (56%), Gaps = 12/170 (7%)
Query: 54 VRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGK 113
V V KDG+G T+ A+ +P N ++ V+ I G Y+EK+ V + V F G
Sbjct: 392 VVVAKDGSGKCKTIAQALAMVPMKNTKKFVIHIKEGVYKEKVEVTKKMLHVMFVG----- 446
Query: 114 DNDMMPIITYDATAL--RYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAM 171
D +IT D L + GT +A+VAV+ DYF+A ++ F N++ + QA+A+
Sbjct: 447 DGPTKTVITGDIAFLPDQVGTYRTASVAVNGDYFMAKDIGFENTA-----GAARHQAVAL 501
Query: 172 RISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIY 221
R+S D A F+NC G+QDTL + F+++C + GT DF+FG+ K+++
Sbjct: 502 RVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVSGTIDFVFGDAKAVF 551
>At5g27870 pectin methyl-esterase - like protein
Length = 732
Score = 110 bits (275), Expect = 1e-24
Identities = 60/173 (34%), Positives = 101/173 (57%), Gaps = 13/173 (7%)
Query: 56 VRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGKDN 115
V +DG+G + T+ +A+ +P VV I G Y+E + V+RS + F G+ G D
Sbjct: 255 VAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGD--GPDK 312
Query: 116 DMMPIITYDATALRYG--TLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRI 173
++ + + + G T +ATVA+ D+F+A N+AF N++ ++ QA+A+R+
Sbjct: 313 TVIS----GSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTA-----GAIKHQAVAIRV 363
Query: 174 SGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTTI 226
D++ FYNCKF G+QDTL + F++DC I GT DF+FG+ +++ T+
Sbjct: 364 LADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTL 416
>At4g02300 putative pectinesterase
Length = 532
Score = 110 bits (275), Expect = 1e-24
Identities = 59/169 (34%), Positives = 97/169 (56%), Gaps = 11/169 (6%)
Query: 59 DGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGKDNDMM 118
DGTG+FTT+ DAV + P+ ++ R +++I GEY E + + + K + F G+ GK
Sbjct: 227 DGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKT---- 282
Query: 119 PIITYDATALR-YGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRISGDK 177
+I + + + + T + TV V ++A +++FVNS+ QA+A R D
Sbjct: 283 -VIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSA-----GPAKAQAVAFRSGSDH 336
Query: 178 AAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTTI 226
+AFY C+F G+QDTL K F+++C I GT DFIFGN ++ +++
Sbjct: 337 SAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSL 385
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.328 0.141 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,927,428
Number of Sequences: 26719
Number of extensions: 332545
Number of successful extensions: 981
Number of sequences better than 10.0: 74
Number of HSP's better than 10.0 without gapping: 70
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 781
Number of HSP's gapped (non-prelim): 104
length of query: 335
length of database: 11,318,596
effective HSP length: 100
effective length of query: 235
effective length of database: 8,646,696
effective search space: 2031973560
effective search space used: 2031973560
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC124967.4