BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC123593.2 + phase: 0 /pseudo
(199 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g45550 putative protein 44 6e-05
At2g31520 putative non-LTR retroelement reverse transcriptase 44 6e-05
At2g25550 putative non-LTR retroelement reverse transcriptase 44 6e-05
At2g17610 putative non-LTR retroelement reverse transcriptase 42 2e-04
At2g01840 putative non-LTR retroelement reverse transcriptase 37 0.005
At4g09710 RNA-directed DNA polymerase -like protein 36 0.015
At1g47910 reverse transcriptase, putative 33 0.076
At2g17910 putative non-LTR retroelement reverse transcriptase 33 0.100
At3g32110 non-LTR reverse transcriptase, putative 33 0.13
At4g10830 putative protein 32 0.17
At2g45230 putative non-LTR retroelement reverse transcriptase 31 0.38
At2g41580 putative non-LTR retroelement reverse transcriptase 31 0.49
At2g15540 putative non-LTR retroelement reverse transcriptase 31 0.49
At2g05200 putative non-LTR retroelement reverse transcriptase 30 0.65
At2g16680 putative non-LTR retroelement reverse transcriptase 30 1.1
At4g07516 putative protein 29 1.4
At4g04000 putative reverse transcriptase 29 1.4
At1g24640 hypothetical protein 27 7.1
At1g73760 putative RING zinc finger protein 27 9.3
>At3g45550 putative protein
Length = 851
Score = 43.9 bits (102), Expect = 6e-05
Identities = 40/130 (30%), Positives = 57/130 (43%), Gaps = 24/130 (18%)
Query: 47 GLCGKRVGWIKDRVFGGNVSILVNGSPTEEIS-----KQGDHLTPF---LCS---RRF** 95
G C K +GWI V + S+L+NGSP IS +QGD L+P+ LC
Sbjct: 218 GFCDKWIGWIMAAVKSVHYSVLINGSPHGYISPTRGIRQGDPLSPYLFILCGDILSHLIK 277
Query: 96 VDVQCGESK*V*GF*GC*RRDGDFTSS-----YADDTLCIGETTVENLYTLKAMLREFEM 150
V G+ + V R G+ + +ADD+L + V N LK + +E
Sbjct: 278 VKASSGDIRGV--------RIGNGAPAITHLQFADDSLFFCQANVRNCQALKDVFDVYEY 329
Query: 151 ASSLKVNFLK 160
S K+N K
Sbjct: 330 YSGQKINVQK 339
>At2g31520 putative non-LTR retroelement reverse transcriptase
Length = 1524
Score = 43.9 bits (102), Expect = 6e-05
Identities = 39/134 (29%), Positives = 56/134 (41%), Gaps = 32/134 (23%)
Query: 47 GLCGKRVGWIKDRVFGGNVSILVNGSPTEEIS-----KQGDHLTPFLCSRRF**VDVQCG 101
G C K +GWI V + S+L+NGSP I+ +QGD L+P+L + CG
Sbjct: 753 GFCNKWIGWIMAAVKSVHYSVLINGSPHGYITPTRGIRQGDPLSPYLF--------ILCG 804
Query: 102 E--SK*V*GF*GC*RRDGDFTS-------------SYADDTLCIGETTVENLYTLKAMLR 146
+ S + G GD +ADD+L + V N LK +
Sbjct: 805 DILSHLINGR----ASSGDLRGVRIGNGAPAITHLQFADDSLFFCQANVRNCQALKDVFD 860
Query: 147 EFEMASSLKVNFLK 160
+E S K+N K
Sbjct: 861 VYEYYSGQKINVQK 874
>At2g25550 putative non-LTR retroelement reverse transcriptase
Length = 1750
Score = 43.9 bits (102), Expect = 6e-05
Identities = 39/134 (29%), Positives = 56/134 (41%), Gaps = 32/134 (23%)
Query: 47 GLCGKRVGWIKDRVFGGNVSILVNGSPTEEIS-----KQGDHLTPFLCSRRF**VDVQCG 101
G C K +GWI V + S+L+NGSP I+ +QGD L+P+L + CG
Sbjct: 979 GFCNKWIGWIMAAVKSVHYSVLINGSPHGYITPTRGIRQGDPLSPYLF--------ILCG 1030
Query: 102 E--SK*V*GF*GC*RRDGDFTS-------------SYADDTLCIGETTVENLYTLKAMLR 146
+ S + G GD +ADD+L + V N LK +
Sbjct: 1031 DILSHLINGR----ASSGDLRGVRIGNGAPAITHLQFADDSLFFCQANVRNCQALKDVFD 1086
Query: 147 EFEMASSLKVNFLK 160
+E S K+N K
Sbjct: 1087 VYEYYSGQKINVQK 1100
>At2g17610 putative non-LTR retroelement reverse transcriptase
Length = 773
Score = 42.4 bits (98), Expect = 2e-04
Identities = 38/126 (30%), Positives = 59/126 (46%), Gaps = 12/126 (9%)
Query: 45 RLGLCGKRVGWIKDRVFGGNVSILVNGSPTEEIS-----KQGDHLTPFL---CSRRF**V 96
++G K WI + SIL+NG P I +QGD ++P+L C+ +
Sbjct: 50 QMGFSEKWCNWIMTCITTTTYSILINGQPVRRIIPKRGIRQGDPISPYLYLLCTEGLSAL 109
Query: 97 DVQCGESK*V*GF*GC*RRDGDFTSS--YADDTLCIGETTVENLYTLKAMLREFEMASSL 154
++K + GF R+G S +A D+L + T+E TL +L+ +E AS
Sbjct: 110 IQASIKAKQLHGFKAS--RNGPAISHLLFAHDSLVFCKATLEECMTLVNVLKLYEKASGQ 167
Query: 155 KVNFLK 160
VNF K
Sbjct: 168 AVNFQK 173
>At2g01840 putative non-LTR retroelement reverse transcriptase
Length = 1715
Score = 37.4 bits (85), Expect = 0.005
Identities = 34/128 (26%), Positives = 61/128 (47%), Gaps = 16/128 (12%)
Query: 45 RLGLCGKRVGWIKDRVFGGNVSILVNGSPTEEI-----SKQGDHLTPFL---CSRRF**V 96
+LG + V WI V + +L+NGSP +I +QGD L+P+L C+ +
Sbjct: 957 QLGFAPRWVKWIMTCVTSVSYEVLINGSPYGKIFPSRGIRQGDPLSPYLFLFCAEVLSNM 1016
Query: 97 DVQCGESK*V*GF*GC*RRDGDFTS----SYADDTLCIGETTVENLYTLKAMLREFEMAS 152
+ +K + G + D + +ADD+L + +N+ L + +++E AS
Sbjct: 1017 LRKAEVNKQIHGM----KITKDCLAISHLLFADDSLFFCRASNQNIEQLALIFKKYEEAS 1072
Query: 153 SLKVNFLK 160
K+N+ K
Sbjct: 1073 GQKINYAK 1080
>At4g09710 RNA-directed DNA polymerase -like protein
Length = 1274
Score = 35.8 bits (81), Expect = 0.015
Identities = 33/124 (26%), Positives = 54/124 (42%), Gaps = 8/124 (6%)
Query: 45 RLGLCGKRVGWIKDRVFGGNVSILVNGSPTEEI-----SKQGDHLTPF---LCSRRF**V 96
RLG K + W+ V + S L+NGSP + +QGD L+P+ LC+ +
Sbjct: 536 RLGFHDKWIRWVMQCVCTVSYSFLINGSPQGSVVPSRGLRQGDPLSPYLFILCTEVLSGL 595
Query: 97 DVQCGESK*V*GF*GC*RRDGDFTSSYADDTLCIGETTVENLYTLKAMLREFEMASSLKV 156
+ E + G +ADDT+ +T L +L+++E+AS +
Sbjct: 596 CRKAQEKGVMVGIRVARGSPQVNHLLFADDTMFFCKTNPTCCGALSNILKKYELASGQSI 655
Query: 157 NFLK 160
N K
Sbjct: 656 NLAK 659
>At1g47910 reverse transcriptase, putative
Length = 1142
Score = 33.5 bits (75), Expect = 0.076
Identities = 29/124 (23%), Positives = 51/124 (40%), Gaps = 8/124 (6%)
Query: 45 RLGLCGKRVGWIKDRVFGGNVSILVNGSP-----TEEISKQGDHLTPF---LCSRRF**V 96
++G C K + WI + +L+NG P E +QGD L+P+ LC+
Sbjct: 379 KMGFCEKWISWIMWCITTVQYKVLINGQPKGLIIPERGLRQGDPLSPYLFILCTEVLIAN 438
Query: 97 DVQCGESK*V*GF*GC*RRDGDFTSSYADDTLCIGETTVENLYTLKAMLREFEMASSLKV 156
+ + G +ADD+L + E + +L+++E S ++
Sbjct: 439 IRKAERQNLITGIKVATPSPAVSHLLFADDSLFFCKANKEQCGIILEILKQYESVSGQQI 498
Query: 157 NFLK 160
NF K
Sbjct: 499 NFSK 502
>At2g17910 putative non-LTR retroelement reverse transcriptase
Length = 1344
Score = 33.1 bits (74), Expect = 0.100
Identities = 31/123 (25%), Positives = 53/123 (42%), Gaps = 8/123 (6%)
Query: 46 LGLCGKRVGWIKDRVFGGNVSILVNGSPTEEIS-----KQGDHLTP---FLCSRRF**VD 97
LG K + WI + V + S+L+NG P I +QGD L+P LC+ +
Sbjct: 574 LGFNNKWISWIMNCVTSVSYSVLINGQPYGHIIPTRGIRQGDPLSPALFVLCTEALIHIL 633
Query: 98 VQCGESK*V*GF*GC*RRDGDFTSSYADDTLCIGETTVENLYTLKAMLREFEMASSLKVN 157
+ ++ + G ++ +ADDTL + + T + L L ++ S +N
Sbjct: 634 NKAEQAGKITGIQFQDKKVSVNHLLFADDTLLMCKATKQECEELMQCLSQYGQLSGQMIN 693
Query: 158 FLK 160
K
Sbjct: 694 LNK 696
>At3g32110 non-LTR reverse transcriptase, putative
Length = 1911
Score = 32.7 bits (73), Expect = 0.13
Identities = 35/127 (27%), Positives = 57/127 (44%), Gaps = 18/127 (14%)
Query: 47 GLCGKRVGWIKDRVFGGNVSILVNGSPTEEIS-----KQGDHLTPF---LCSRRF**V-- 96
GL G V WI V G ++ +L NG T+ +QGD L+P+ LC R +
Sbjct: 1136 GLPGTWVQWIMKCVEGPSMRLLWNGEKTDAFKPLRGLRQGDPLSPYLFVLCIERLCHLIE 1195
Query: 97 -DVQCGESK*V*GF*GC*RRDGDFTS--SYADDTLCIGETTVENLYTLKAMLREFEMASS 153
+ + K + + G S +ADD + E +++ + L+ +L +F AS
Sbjct: 1196 SSIAAKKWKPI-----KISQSGPRLSHICFADDLILFAEASIDQIRVLRGVLEKFCGASG 1250
Query: 154 LKVNFLK 160
KV+ K
Sbjct: 1251 QKVSLEK 1257
>At4g10830 putative protein
Length = 1294
Score = 32.3 bits (72), Expect = 0.17
Identities = 33/116 (28%), Positives = 48/116 (40%), Gaps = 8/116 (6%)
Query: 53 VGWIKDRVFGGNVSILVNGSPTEEIS-----KQGDHLTPF---LCSRRF**VDVQCGESK 104
+ WI V N S+LVNG P I +QGD L+P+ LC+ +
Sbjct: 965 IKWIMGAVKSVNYSVLVNGIPHGTIQPQRGIRQGDPLSPYLFILCADILNHLIKNRVAEG 1024
Query: 105 *V*GF*GC*RRDGDFTSSYADDTLCIGETTVENLYTLKAMLREFEMASSLKVNFLK 160
+ G G +ADD+L ++ V N LK + +E S K+N K
Sbjct: 1025 DIRGIRIGNGVPGVTHLQFADDSLFFCQSNVRNCQALKDVFDVYEYYSGQKINMSK 1080
>At2g45230 putative non-LTR retroelement reverse transcriptase
Length = 1374
Score = 31.2 bits (69), Expect = 0.38
Identities = 30/123 (24%), Positives = 53/123 (42%), Gaps = 8/123 (6%)
Query: 46 LGLCGKRVGWIKDRVFGGNVSILVNGSPTEEIS-----KQGDHLTPFL---CSRRF**VD 97
LG + I + V +L+NG+P EI +QGD L+P+L C+ +
Sbjct: 596 LGFADHWIRLIMECVKSVRYQVLINGTPHGEIIPSRGLRQGDPLSPYLFVICTEMLVKML 655
Query: 98 VQCGESK*V*GF*GC*RRDGDFTSSYADDTLCIGETTVENLYTLKAMLREFEMASSLKVN 157
+ + G +ADD++ + E L + ++ E+ +AS +VN
Sbjct: 656 QSAEQKNQITGLKVARGAPPISHLLFADDSMFYCKVNDEALGQIIRIIEEYSLASGQRVN 715
Query: 158 FLK 160
+LK
Sbjct: 716 YLK 718
>At2g41580 putative non-LTR retroelement reverse transcriptase
Length = 1094
Score = 30.8 bits (68), Expect = 0.49
Identities = 17/48 (35%), Positives = 25/48 (51%), Gaps = 5/48 (10%)
Query: 46 LGLCGKRVGWIKDRVFGGNVSILVNGSPTEEIS-----KQGDHLTPFL 88
LG + W+ V + S+L+NG P I+ +QGD L+PFL
Sbjct: 325 LGFNSTWINWMMACVSSVSYSVLINGQPFGHITPHRGLRQGDPLSPFL 372
>At2g15540 putative non-LTR retroelement reverse transcriptase
Length = 1225
Score = 30.8 bits (68), Expect = 0.49
Identities = 18/49 (36%), Positives = 26/49 (52%), Gaps = 5/49 (10%)
Query: 45 RLGLCGKRVGWIKDRVFGGNVSILVNGSPTEEIS-----KQGDHLTPFL 88
++G K V WI + + IL+NGSP I +QGD ++PFL
Sbjct: 542 KMGFAQKWVDWIIFCISSVSYKILLNGSPKGFIKPSRGIRQGDPISPFL 590
>At2g05200 putative non-LTR retroelement reverse transcriptase
Length = 1229
Score = 30.4 bits (67), Expect = 0.65
Identities = 29/124 (23%), Positives = 52/124 (41%), Gaps = 8/124 (6%)
Query: 45 RLGLCGKRVGWIKDRVFGGNVSILVNGSPTEEI-----SKQGDHLTP---FLCSRRF**V 96
R G + W+ + V + S L+NG+P ++ +QGD L+P LC+ +
Sbjct: 490 RFGFHSIWIDWVLECVTSVSYSFLINGTPQGKVVPTRGLRQGDPLSPCLFILCTEVLSGL 549
Query: 97 DVQCGESK*V*GF*GC*RRDGDFTSSYADDTLCIGETTVENLYTLKAMLREFEMASSLKV 156
+ + + G +ADDT+ ++ E+ L +L + AS +
Sbjct: 550 CTRAQRLRQLPGVRVSINGPRVNHLLFADDTMFFSKSDPESCNKLSEILSRYGKASGQSI 609
Query: 157 NFLK 160
NF K
Sbjct: 610 NFHK 613
>At2g16680 putative non-LTR retroelement reverse transcriptase
Length = 1319
Score = 29.6 bits (65), Expect = 1.1
Identities = 17/48 (35%), Positives = 24/48 (49%), Gaps = 5/48 (10%)
Query: 46 LGLCGKRVGWIKDRVFGGNVSILVNGSPTEEIS-----KQGDHLTPFL 88
LG K + WI V S+L+NG I+ +QGD ++PFL
Sbjct: 548 LGFNDKWISWIMGCVTSVTYSVLINGQHFGHITPERGIRQGDPISPFL 595
>At4g07516 putative protein
Length = 740
Score = 29.3 bits (64), Expect = 1.4
Identities = 15/41 (36%), Positives = 23/41 (55%), Gaps = 5/41 (12%)
Query: 53 VGWIKDRVFGGNVSILVNGSPTEEIS-----KQGDHLTPFL 88
+ WI V G ++S++ NG TE +QGD L+P+L
Sbjct: 255 IWWIMQCVTGPSMSLMWNGEKTEPFRPSRGLRQGDPLSPYL 295
>At4g04000 putative reverse transcriptase
Length = 1077
Score = 29.3 bits (64), Expect = 1.4
Identities = 13/38 (34%), Positives = 23/38 (60%)
Query: 123 YADDTLCIGETTVENLYTLKAMLREFEMASSLKVNFLK 160
+ADDT+ ++ LK++L ++E+AS K+N K
Sbjct: 616 FADDTMFFCRVDPKSCLKLKSILHKYELASGQKINQAK 653
>At1g24640 hypothetical protein
Length = 1270
Score = 26.9 bits (58), Expect = 7.1
Identities = 18/48 (37%), Positives = 23/48 (47%), Gaps = 5/48 (10%)
Query: 46 LGLCGKRVGWIKDRVFGGNVSILVNGSP-----TEEISKQGDHLTPFL 88
LG K V WI V S+L+N P + +QGD L+PFL
Sbjct: 559 LGFHLKWVNWIMVCVSSVTYSVLINDCPFGLIILQRGLRQGDPLSPFL 606
>At1g73760 putative RING zinc finger protein
Length = 367
Score = 26.6 bits (57), Expect = 9.3
Identities = 11/29 (37%), Positives = 16/29 (54%)
Query: 49 CGKRVGWIKDRVFGGNVSILVNGSPTEEI 77
CG VG+ D V GG++ +V+ P I
Sbjct: 146 CGPGVGFSTDAVVGGSIDTVVSDPPRRNI 174
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.363 0.169 0.619
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,787,147
Number of Sequences: 26719
Number of extensions: 130626
Number of successful extensions: 608
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 583
Number of HSP's gapped (non-prelim): 35
length of query: 199
length of database: 11,318,596
effective HSP length: 94
effective length of query: 105
effective length of database: 8,807,010
effective search space: 924736050
effective search space used: 924736050
T: 11
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (22.0 bits)
S2: 57 (26.6 bits)
Medicago: description of AC123593.2