BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122169.4 - phase: 0
(165 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g41180 unknown protein 75 2e-14
At3g56710 SigA binding protein 67 4e-12
At2g42140 putative protein 37 0.004
At3g58000 putative protein 35 0.024
At5g01370 unknown protein 34 0.031
At1g78410 unknown protein 33 0.053
At3g60090 putative protein 32 0.12
At2g44340 hypothetical protein 32 0.12
At3g01770 unknown protein 31 0.26
At1g68450 unknown protein 31 0.35
At3g18690 unknown protein 30 0.45
At5g60380 putative protein 30 0.59
At2g33880 homeodomain transcription like factor 30 0.59
At3g10660 calmodulin-domain protein kinase CDPK isoform 2 30 0.77
At5g58080 ARR2 - like protein 29 1.0
At5g40690 unknown protein 29 1.0
At4g36890 UDP-glucuronyltransferase-like protein 29 1.0
At5g04690 putative protein 29 1.3
At1g35830 hypothetical protein 29 1.3
At5g65170 putative protein 28 1.7
>At2g41180 unknown protein
Length = 141
Score = 74.7 bits (182), Expect = 2e-14
Identities = 46/104 (44%), Positives = 65/104 (62%), Gaps = 8/104 (7%)
Query: 10 SSSVISTVQQKTPTKLTK-PKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFRALVQELT 68
SSS + Q+K+ + T+ P K+K + T +KPIKV YISNPM+V+T S+FR LVQELT
Sbjct: 4 SSSTLLINQRKSSSSPTRIPPKQKRKSTTTHKPIKVRYISNPMRVETCPSKFRELVQELT 63
Query: 69 GQ-YAESPPNPSRFQEFDVNDSGTDQGGCENMMDCDKSDQTVVG 111
GQ A+ PP+P+ F D++ CE+ M+ + D V G
Sbjct: 64 GQDAADLPPSPTTFTAVDLHRP------CESEMNLEPLDGEVRG 101
>At3g56710 SigA binding protein
Length = 151
Score = 67.0 bits (162), Expect = 4e-12
Identities = 41/76 (53%), Positives = 52/76 (67%), Gaps = 3/76 (3%)
Query: 5 STSSTSSSVISTVQQK-TPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFRAL 63
S+SST + S ++K +P PK+KK +T NKPIKV YISNPM+V+T AS+FR L
Sbjct: 3 SSSSTFLTTTSLDKKKPSPVSRKSPKQKKKTTST-NKPIKVRYISNPMRVQTCASKFREL 61
Query: 64 VQELTGQYA-ESPPNP 78
VQELTGQ A + P P
Sbjct: 62 VQELTGQDAVDLQPEP 77
>At2g42140 putative protein
Length = 172
Score = 37.4 bits (85), Expect = 0.004
Identities = 36/137 (26%), Positives = 57/137 (41%), Gaps = 21/137 (15%)
Query: 15 STVQQKTPTKLTKPKKKKNNNNTYNKP-IKVVYISNPMKVKTSASEFRALVQELTGQ--- 70
+T QK + T K++ T +KP I++++I P +KT + FR +VQ LTG+
Sbjct: 5 ATTVQKRRSLPTIAMHKQSRTLTKSKPKIRIIHIFAPEIIKTDVANFREIVQNLTGKQDH 64
Query: 71 ----------YAESPPNPSRFQEFDVNDSGTDQGGCENMMDCDKSDQTVVGVPSLVDPDD 120
+P + +V+D G C N SD+ G+ S+ +
Sbjct: 65 HHHDLPHQKGLKRNPRSRRSHDHHEVHDMNKSHGFCIN------SDEEEEGMVSMT-WNG 117
Query: 121 KGKPSEAGSSNESFDED 137
G S G N D D
Sbjct: 118 NGDESSGGFLNGLGDLD 134
>At3g58000 putative protein
Length = 175
Score = 34.7 bits (78), Expect = 0.024
Identities = 22/70 (31%), Positives = 33/70 (46%), Gaps = 10/70 (14%)
Query: 1 MDSISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEF 60
M++ SS+ S K +TK K K I++++I P +KT + F
Sbjct: 1 MEATIFEKRRSSLSSIAVHKQSYSITKSKPK----------IRIIHIFAPEIIKTDVANF 50
Query: 61 RALVQELTGQ 70
R LVQ LTG+
Sbjct: 51 RELVQSLTGK 60
>At5g01370 unknown protein
Length = 427
Score = 34.3 bits (77), Expect = 0.031
Identities = 25/74 (33%), Positives = 33/74 (43%), Gaps = 8/74 (10%)
Query: 22 PTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFRAL-------VQELTGQYAES 74
P K+ K KKK N N N KV + +P K K+S+ + L E G+ +ES
Sbjct: 228 PRKVAK-KKKSENRNDVNGAKKVRWFLSPSKSKSSSEKTALLGESKNIRADEFIGKESES 286
Query: 75 PPNPSRFQEFDVND 88
P N S V D
Sbjct: 287 PENKSNASPVSVLD 300
Score = 31.2 bits (69), Expect = 0.26
Identities = 19/62 (30%), Positives = 30/62 (47%), Gaps = 4/62 (6%)
Query: 2 DSISTSSTSSSVISTVQQKT----PTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSA 57
DS S S S+ + T + + P K KK+NNN+T N + +I+ M + +
Sbjct: 319 DSESASELSTKQVETATKSSCSSSPAKTKTSTKKENNNSTNNDSEETEFITKLMNMLSDL 378
Query: 58 SE 59
SE
Sbjct: 379 SE 380
>At1g78410 unknown protein
Length = 108
Score = 33.5 bits (75), Expect = 0.053
Identities = 17/34 (50%), Positives = 25/34 (73%), Gaps = 1/34 (2%)
Query: 39 NKPIKVVYISNPMKVKTSASEFRALVQELTGQYA 72
++P+KVV+I N V+T A F+ +VQELTG+ A
Sbjct: 9 SEPMKVVFI-NTQYVETDARSFKTVVQELTGKNA 41
>At3g60090 putative protein
Length = 157
Score = 32.3 bits (72), Expect = 0.12
Identities = 18/49 (36%), Positives = 26/49 (52%), Gaps = 1/49 (2%)
Query: 23 TKLTKPKKKKNNNNTYNKP-IKVVYISNPMKVKTSASEFRALVQELTGQ 70
+K P K + T KP I++++I P + T FR LVQ LTG+
Sbjct: 25 SKTLVPMNKNSQIITKIKPKIRIIHIFAPEIINTDVKNFRTLVQSLTGK 73
>At2g44340 hypothetical protein
Length = 188
Score = 32.3 bits (72), Expect = 0.12
Identities = 14/31 (45%), Positives = 21/31 (67%)
Query: 42 IKVVYISNPMKVKTSASEFRALVQELTGQYA 72
I++++I P +KT FR+LVQ LTG+ A
Sbjct: 33 IRIIHIFAPEVIKTDVKNFRSLVQSLTGKPA 63
>At3g01770 unknown protein
Length = 620
Score = 31.2 bits (69), Expect = 0.26
Identities = 22/77 (28%), Positives = 35/77 (44%), Gaps = 2/77 (2%)
Query: 85 DVNDSGTDQ-GGCENMMDCDKSDQTVVGVPSLVDPDDKGKPSEAGSSNESFDEDVLLMPE 143
D N + D GGC++ + K D+T GVP +V P SE + +++ +
Sbjct: 11 DYNRNSFDSPGGCDDSPNASKDDETF-GVPRIVLPLSDLSSSERRKWIHTLRQELEQLRS 69
Query: 144 MMDNIWDLLPTSAFYES 160
++ DLLP S S
Sbjct: 70 FQKSVGDLLPISKIVTS 86
>At1g68450 unknown protein
Length = 152
Score = 30.8 bits (68), Expect = 0.35
Identities = 17/46 (36%), Positives = 24/46 (51%), Gaps = 1/46 (2%)
Query: 24 KLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFRALVQELTG 69
K T K K + P+ ++Y +P + T A +F ALVQ LTG
Sbjct: 27 KKTSSCKSKPRPHGRASPV-IIYAHSPKVIHTRAEDFMALVQRLTG 71
>At3g18690 unknown protein
Length = 222
Score = 30.4 bits (67), Expect = 0.45
Identities = 19/52 (36%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Query: 20 KTPTKLTKPKKKKNNNNTY--NKPIKVVYISNPMKVKTSASEFRALVQELTG 69
K P K P ++ Y +P+ V+Y +P V +ASEF +VQ LTG
Sbjct: 42 KKPPKHPAPPPNRDQPPPYIPREPV-VIYAVSPKVVHATASEFMNVVQRLTG 92
>At5g60380 putative protein
Length = 373
Score = 30.0 bits (66), Expect = 0.59
Identities = 21/65 (32%), Positives = 30/65 (45%), Gaps = 7/65 (10%)
Query: 16 TVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFRALVQELTGQY---- 71
T+Q K + K + +NN TY K I+ Y + P V + EF Q L +Y
Sbjct: 63 TIQMKPSLSRHELKNQTSNNKTYKKDIECPYGTIP--VLRNTKEFNTKAQLLAAKYFNPL 120
Query: 72 -AESP 75
A+SP
Sbjct: 121 SADSP 125
>At2g33880 homeodomain transcription like factor
Length = 378
Score = 30.0 bits (66), Expect = 0.59
Identities = 17/35 (48%), Positives = 22/35 (62%), Gaps = 8/35 (22%)
Query: 3 SISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNT 37
S S+SS+SSS + +K TKP+K KN NNT
Sbjct: 138 SASSSSSSSS--------SSSKSTKPRKSKNKNNT 164
>At3g10660 calmodulin-domain protein kinase CDPK isoform 2
Length = 646
Score = 29.6 bits (65), Expect = 0.77
Identities = 25/123 (20%), Positives = 46/123 (37%), Gaps = 8/123 (6%)
Query: 4 ISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFRAL 63
+S + S VQ K P +T P K N +++ KV ++ + +
Sbjct: 43 VSKEAASEPATDQVQNKPPEPITMPSSKTNPETKLKPDLEIQPEEKKEKVLAEETKQKVV 102
Query: 64 VQELTGQYAESPPNPSRFQEFDVNDSGTDQGGCENMMDCDKSDQTVVGVPSLVDPDDKGK 123
+E E PP S+ + +S + E+ + K + T S P+ K +
Sbjct: 103 PEE---SKQEVPPEESKREVVVQPESAKPETKSESKPETTKPETT-----SETKPETKAE 154
Query: 124 PSE 126
P +
Sbjct: 155 PQK 157
>At5g58080 ARR2 - like protein
Length = 632
Score = 29.3 bits (64), Expect = 1.0
Identities = 21/71 (29%), Positives = 37/71 (51%), Gaps = 4/71 (5%)
Query: 76 PNPSRFQEFDVNDSGTDQGGCENMMD-CDKSDQTVVGVPSLVDPDDKGKPSEAGSSNESF 134
P P+ F N+S +G E++M+ D S + V +++DP+ K E + ++ F
Sbjct: 542 PLPADNMVFRDNNSTRSKGLDESLMNPIDNSQEYVGKATTMLDPEMKSGKPENDNQHDVF 601
Query: 135 DEDVLLMPEMM 145
D+ +M EMM
Sbjct: 602 DD---IMNEMM 609
>At5g40690 unknown protein
Length = 210
Score = 29.3 bits (64), Expect = 1.0
Identities = 26/97 (26%), Positives = 43/97 (43%), Gaps = 15/97 (15%)
Query: 52 KVKTSASEFRALVQELTGQYAESPPNP----SRFQEFDVNDSGTDQGGCENMMDCDKSDQ 107
KV+T+ ++ L T Q A P P S F + D +D G ++ D D+ ++
Sbjct: 84 KVRTNDVTYKML----TWQVAAGGPRPGLKLSGFSATGILDLMSDDYGYDHDYDYDEENE 139
Query: 108 -------TVVGVPSLVDPDDKGKPSEAGSSNESFDED 137
V V ++ DD G+ E GS+++ D D
Sbjct: 140 EEENRGSVVEEVVNIQSSDDGGETEEEGSNDDDDDTD 176
>At4g36890 UDP-glucuronyltransferase-like protein
Length = 525
Score = 29.3 bits (64), Expect = 1.0
Identities = 13/40 (32%), Positives = 22/40 (54%)
Query: 2 DSISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKP 41
D + S ++ V+ QQ+ PTK+ KPK+K + +P
Sbjct: 468 DQMPLSQGNTVVVIPKQQQHPTKIRKPKRKSKKSKHEPRP 507
>At5g04690 putative protein
Length = 625
Score = 28.9 bits (63), Expect = 1.3
Identities = 14/45 (31%), Positives = 22/45 (48%)
Query: 31 KKNNNNTYNKPIKVVYISNPMKVKTSASEFRALVQELTGQYAESP 75
K + NN YN P+ VV +S M++ + + E+ G E P
Sbjct: 140 KMSQNNFYNTPLTVVAVSGNMEIAEALVAKNPKLLEIPGNNGEIP 184
>At1g35830 hypothetical protein
Length = 302
Score = 28.9 bits (63), Expect = 1.3
Identities = 18/40 (45%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Query: 55 TSASEFRALVQELTGQYAE--SPPNPSRFQEFDVNDSGTD 92
T S FRA+VQE TG A S P S + FD+ S +D
Sbjct: 116 TDTSNFRAMVQEFTGVPASPFSHPFSSTTRRFDIFRSPSD 155
>At5g65170 putative protein
Length = 362
Score = 28.5 bits (62), Expect = 1.7
Identities = 20/45 (44%), Positives = 23/45 (50%), Gaps = 3/45 (6%)
Query: 28 PKKKKNNNNTYNKPIKVVYISN--PMKVKTS-ASEFRALVQELTG 69
P KKN T P K +S P V T+ S FRA+VQE TG
Sbjct: 126 PTDKKNGLATTRNPKKRSRVSRRAPTTVLTTDTSNFRAMVQEFTG 170
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.306 0.125 0.346
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,068,115
Number of Sequences: 26719
Number of extensions: 181970
Number of successful extensions: 633
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 608
Number of HSP's gapped (non-prelim): 55
length of query: 165
length of database: 11,318,596
effective HSP length: 92
effective length of query: 73
effective length of database: 8,860,448
effective search space: 646812704
effective search space used: 646812704
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC122169.4