BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121241.8 - phase: 0
(972 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52950 putative protein 503 e-142
At2g06950 putative retroelement pol polyprotein 37 0.054
At1g69270 putative receptor-like protein kinase 33 0.78
At3g09070 unknown protein 32 1.7
At1g04980 isulfide isomerase-related like protein 32 2.3
At5g03640 protein kinase -like protein 31 3.0
At5g62360 DC1.2 homologue - like protein 31 3.9
At5g01400 putative protein 31 3.9
At1g72000 putative invertase 31 3.9
At2g32920 putative protein disulfide isomerase 30 5.1
At1g79540 unknown protein 30 5.1
At4g37400 cytochrome P450 monooxygenase -like protein 30 6.6
At1g17030 unknown protein 30 6.6
At1g22650 invertase like protein 30 8.6
>At5g52950 putative protein
Length = 945
Score = 503 bits (1296), Expect = e-142
Identities = 358/994 (36%), Positives = 518/994 (52%), Gaps = 110/994 (11%)
Query: 11 YSHTNRIVLLIDLDPLLLLLHNTTTTTNYIKNILSTSKTLFSFPPLSTSLFAFKFFFSSL 70
Y+ T R VLLIDL+PLL+ + Y+ ++S ++ L FPPLS SLF+FKFFFSSL
Sbjct: 7 YAKTQRFVLLIDLNPLLI----KPNSEQYLAVVISAAEKLLLFPPLSASLFSFKFFFSSL 62
Query: 71 PPHLSFSKLHPF-LPKHSFSFDHPFSTFNLLSKTLSTFPNFPL--------TYNPKAANL 121
LS SKL + SFD P T L + + + L +P+ N+
Sbjct: 63 SSLLSSSKLSSLSISSSPLSFDLPNPTLVSLKRAIDAVKGYELRSSSTVATAASPRGVNV 122
Query: 122 IDSLTQLLHDYPWEPN-SDAD-------TDTTTPLVSPNLILLFTPFFNSFNSLAGFFD- 172
+L Q+++DY WEP D + TD +V NL+++F+P + ++ F D
Sbjct: 123 AANLRQIVYDYAWEPVVRDPEIGMIPGFTDGGLDVVRSNLVVMFSPISRDLDWVSEFLDV 182
Query: 173 -SDEDSLRIENSFCDRFLGFFGNVSRRFRSKGVHCSWIGVNSDDKEDEVGMIRGLFEIGT 231
S ++ + F + F V+ F + + SWI V S D+ E+G+ G F+ G
Sbjct: 183 KSGDECFSDLDLFKSKLREIFYCVNELFDDRDIQLSWIDVKSGDERCELGLKSGFFDSGI 242
Query: 232 GKLGWGFCSLDSILLGSALVPFGLIYPKIGVSWISVRCCSREVKVQLTLEILDVNGSPIE 291
+LGWG CS DSI+ GS++VPFGLIYP IG+S S++ +Q +LEI D+NG P+E
Sbjct: 243 RELGWGHCSTDSIVFGSSVVPFGLIYPAIGIS--PKLSTSQKFTLQASLEIADINGKPME 300
Query: 292 YNC-CDLEVLDFRVFGR---GEDVNLQGGG---RKERLWNVCSDGMAKLKVTVVRKCDAF 344
C +LE +F E +NL G + L DG KL + +R CD
Sbjct: 301 CKCGGELEFSSSEIFSGKRCDEFINLASGTEPVNHDSLVEQFCDGSTKLSIKALRMCDDL 360
Query: 345 VKFRSCLSDSVLVREVLGECMKG--DSGGFFADRVLELLATEFGCQGRRKSVPVWEMLLS 402
++ D+ +V +V + + + F+AD V ++L E G + ++S P+W++LLS
Sbjct: 361 IELERYTCDTFVVHQVSQDSDQDQEEESAFWADLVFQILGKETGERVAKRSSPIWQILLS 420
Query: 403 YLYKEDCWALVSVDSGKGGGSCVGILRPFTVSSALLSVLEDPQSASDFGAANMNSFIRTG 462
YLY+E ALVS S G GIL+PFT SSAL+ V ++ S
Sbjct: 421 YLYREGFSALVSF-SNSNGSLRTGILKPFTFSSALICVFDNGVSP--------------- 464
Query: 463 ILKSDRIFHKNRDLVDSQVKDVVGIKGEQKKKMTDLSALRNLTWSSFYDLVYDQFEMDLH 522
+ D DS+ K + + K+ ++L +++W F V D ++DL
Sbjct: 465 ---------QTVDHEDSRKK----VSCSEYKRKPRKNSLNDISWEEFCRSVKDYGQIDLE 511
Query: 523 EVYYAMECNKSKKLKFLKCWMKQVKK------SSCHDLNLSEYPKPNQIFAEGTDSKLNE 576
+VY+ + +KSKKLKFLKCWMKQ+ K S + N E N I + S E
Sbjct: 512 DVYF-FKYSKSKKLKFLKCWMKQISKPRGCSLSVASNCNALEDVDANPIEEKNNSS---E 567
Query: 577 LPQNGEQPIPQVVMSAGINA-EVDTKKDDAVLDCGLETSEAFFRNLSNRIQQGIESDVID 635
P+P ++ + K++ E+SE FF +L ++I+QGIES+ ID
Sbjct: 568 ETDKASSPLPLAEEDIALSGNRISGKQESNTYVHASESSENFFVSLPSKIKQGIESEEID 627
Query: 636 LVALAERLVNSSIYWLCQKVDRETIPLIQVHSPLKDNNACGS----MVVSELVKQLLKDP 691
L ALAERLV S +++ Q+ +++ +C S +V+ EL K LLKDP
Sbjct: 628 LSALAERLVKSCVFYSSQRAEKD--------------YSCESGTLLLVIDELTKMLLKDP 673
Query: 692 KDIAAKHKSRDSSSQA----FDAAGPTIRYELQILFRLEILQSEVGRGIEDSSKQKFVKQ 747
KD+ AK K +DSSS A D AGPTIRYELQILFR+EIL+ + G G E+S QKF KQ
Sbjct: 674 KDLVAKFKKKDSSSVASERNCDEAGPTIRYELQILFRMEILRCKKGLGNEESVTQKFAKQ 733
Query: 748 ICLLLENIQCHMEGGFFGDWNLENYVAKIIKSRYSHTLEDIVHKIYNKMDLLLFVNEDEA 807
IC+ LE IQC ++GGFFG+W+L+ YV K IK+RY H L V+ IY +MDLL+F +ED
Sbjct: 734 ICMFLEGIQCKLDGGFFGEWSLDKYVDKTIKARYQHILGGAVNIIYTEMDLLMFTDED-L 792
Query: 808 PDCSFNSEDSSKSLDRKFYGD-EMGENDVGNGPFSAENKPFHLQKNVRGKLQRNIEGGHN 866
D N+EDSS+S + + + + N +K + +++ K + +G
Sbjct: 793 EDSFMNNEDSSQSGRENIHSNFKNHHHSQSNEDVPGTSKQKNTEESREAKKEVEAQGMRE 852
Query: 867 KT-LIEALKIRMPAL-RVWAPKQ-KGMKSKKDHLSKIPKR-KDRTSACYDTVCETPMT-- 920
+ + MP L RVWAPKQ K K K D + KR K++ S YD VCETPMT
Sbjct: 853 RARRFSSFTSWMPDLCRVWAPKQTKNSKGKADQQQRTAKRKKEQRSVEYDRVCETPMTTI 912
Query: 921 RNTRSMTRNT---VCETPMTRSTRSSPQSIGSDD 951
R T N CET + RS S P+++ DD
Sbjct: 913 ETKRIRTGNKDDYECET-LPRS--SVPKALFQDD 943
>At2g06950 putative retroelement pol polyprotein
Length = 405
Score = 37.0 bits (84), Expect = 0.054
Identities = 30/105 (28%), Positives = 47/105 (44%), Gaps = 5/105 (4%)
Query: 784 TLEDIVHKIYNKMDLLLFVNEDEA--PDCSFN---SEDSSKSLDRKFYGDEMGENDVGNG 838
+LED++ K+ D L E+ A P +FN S+DS+ S Y G+++ G
Sbjct: 206 SLEDVIPKLTGYDDRLQGYLEETAVSPHVAFNITTSDDSNASGYFNAYNRGKGKSNRGRN 265
Query: 839 PFSAENKPFHLQKNVRGKLQRNIEGGHNKTLIEALKIRMPALRVW 883
FS + FH Q + + GG + K+ PAL+ W
Sbjct: 266 SFSTRGRGFHQQISSTNSSSGSQSGGTSVVCQICGKMGHPALKCW 310
>At1g69270 putative receptor-like protein kinase
Length = 540
Score = 33.1 bits (74), Expect = 0.78
Identities = 43/150 (28%), Positives = 63/150 (41%), Gaps = 17/150 (11%)
Query: 153 LILLFTPFFNSFN-----SLAGFFDSDEDSLRIENSFCDRFLGFFGNVSRRFRSKGVHCS 207
+ LLF F SF+ G + L++++SF D VS S HCS
Sbjct: 7 VFLLFNLFMFSFSRKLLTESGGGLHDEAALLKLKSSFLDPNGVLSSWVSD---SSSNHCS 63
Query: 208 WIGVNSDDKEDEVGMI-RGLFEI-GTGKL---GWGFCSLDSILLGSALVPFGLIYPKIGV 262
W GV+ + V +I RG E+ G+G L CS LG + P +I V
Sbjct: 64 WYGVSCNSDSRVVSLILRGCDELEGSGVLHLPDLSSCSSSKRRLGGVISPVVGDLSEIRV 123
Query: 263 SWISVRCCSREVKVQL----TLEILDVNGS 288
+S E+ ++ LEILD+ G+
Sbjct: 124 LSLSFNDLRGEIPKEIWGLEKLEILDLKGN 153
>At3g09070 unknown protein
Length = 685
Score = 32.0 bits (71), Expect = 1.7
Identities = 29/103 (28%), Positives = 42/103 (40%), Gaps = 6/103 (5%)
Query: 409 CWALVSVDSGKGGGSCVGILRPFTVSSALLSVLEDPQSASDFGAANMNSFIRTGILKSDR 468
C L +D GGS +P T+S+A L L P + G N N G +K
Sbjct: 48 CERLSVLDQTNNGGSSSSSKKPPTISAAALKALFKPSGNNGVGGVNTNG---NGRVKPGF 104
Query: 469 IFHKNRDLVDSQVKDVVGIKG--EQKKKMTDLSALRNLTWSSF 509
R S K+ G G E +++ D+ LR+ W+ F
Sbjct: 105 FPELRRTKSFSASKNNEGFSGVFEPQRRSCDV-RLRSSLWNLF 146
>At1g04980 isulfide isomerase-related like protein
Length = 443
Score = 31.6 bits (70), Expect = 2.3
Identities = 37/150 (24%), Positives = 60/150 (39%), Gaps = 8/150 (5%)
Query: 387 CQGRRKSVPVWEMLLSYLYKEDCWALVSVDSGKGGGSCVGILRPFTVSSALLSVLEDPQS 446
C + P WE + S L A + D+ K G+ R F + V +
Sbjct: 58 CGHCQSLTPTWEKVASTLKGIATVAAIDADAHKSVSQDYGV-RGFPT----IKVFVPGKP 112
Query: 447 ASDFGAANMNSFIRTGILKSDRIFHKNRDLVDSQVKDVVGIKGEQKKKMTDLSALRNLTW 506
D+ A I +K + K+R +D + G +KK ++ SA L
Sbjct: 113 PIDYQGARDAKSISQFAIKQIKALLKDR--LDGKTSGTKNGGGSSEKKKSEPSASVELNS 170
Query: 507 SSFYDLVYDQFEMDLHEVYYAMECNKSKKL 536
S+F +LV + E+ + E ++A C KKL
Sbjct: 171 SNFDELVTESKELWIVE-FFAPWCGHCKKL 199
>At5g03640 protein kinase -like protein
Length = 926
Score = 31.2 bits (69), Expect = 3.0
Identities = 20/66 (30%), Positives = 32/66 (48%), Gaps = 4/66 (6%)
Query: 534 KKLKFLKCWMKQVKKSSCHDLNLSEYPKPNQIFAEGTDSKLNELPQNGEQPIPQVVMSAG 593
KK K + MK++KKS ++ + Q+ AE TD++ N E P+++ G
Sbjct: 739 KKGKQHRMMMKKLKKSDL----IARFKSLPQLVAEPTDARSNSFVGTHEYLAPEIIKGEG 794
Query: 594 INAEVD 599
A VD
Sbjct: 795 HGAAVD 800
>At5g62360 DC1.2 homologue - like protein
Length = 203
Score = 30.8 bits (68), Expect = 3.9
Identities = 22/78 (28%), Positives = 37/78 (47%), Gaps = 8/78 (10%)
Query: 11 YSHTNRIVLLIDLDPLLLLLHNTTTTTNYIKNILSTSKTLFSFPPLSTSLFAF-KFFFSS 69
++H + + + + +L L+H TTTTT +T+ T F S + + + FSS
Sbjct: 8 FNHHHFLTTFLIIIAMLKLVHTTTTTT-------TTTTTNTEFVKSSCTFTTYPRLCFSS 60
Query: 70 LPPHLSFSKLHPFLPKHS 87
L H S + P L H+
Sbjct: 61 LSTHASLIQTSPKLMAHA 78
>At5g01400 putative protein
Length = 1467
Score = 30.8 bits (68), Expect = 3.9
Identities = 18/58 (31%), Positives = 28/58 (48%)
Query: 427 ILRPFTVSSALLSVLEDPQSASDFGAANMNSFIRTGILKSDRIFHKNRDLVDSQVKDV 484
++R SS LL ++ DP S SD + + G S + R L D+++KDV
Sbjct: 977 LVRTMGSSSELLKIIADPPSGSDNLLIQVLQTLTEGPTPSSELILTIRKLFDTRIKDV 1034
>At1g72000 putative invertase
Length = 499
Score = 30.8 bits (68), Expect = 3.9
Identities = 20/67 (29%), Positives = 34/67 (49%), Gaps = 1/67 (1%)
Query: 718 ELQILFRLEILQSEVGRGIEDSSKQKFVKQICLLLENIQCHMEGGFFGDWNLENYVAKII 777
E+Q LF + L+S + DS ++F+++I L + HM F+ D+ N + +
Sbjct: 224 EIQALFFMA-LRSALSMLKHDSEGKEFMEKIVKRLHALSFHMRSYFWLDFQQLNDIYRYK 282
Query: 778 KSRYSHT 784
YSHT
Sbjct: 283 TEEYSHT 289
>At2g32920 putative protein disulfide isomerase
Length = 440
Score = 30.4 bits (67), Expect = 5.1
Identities = 38/155 (24%), Positives = 63/155 (40%), Gaps = 21/155 (13%)
Query: 387 CQGRRKSVPVWEMLLSYLYKEDCWALVSVDSGKGGGSCVGI-----LRPFTVSSALLSVL 441
C + P WE + + L A + D+ + GI ++ F A +
Sbjct: 60 CGHCKALTPTWEKVANILKGVATVAAIDADAHQSAAQDYGIKGFPTIKVFVPGKAPI--- 116
Query: 442 EDPQSASDFGAANMNSFIRTGILKSDRIFHKNRDLVDSQVKDVVGIKGEQKKKMTDLSAL 501
D Q A D + ++ + L SDR+ K++ G K+K ++ SA
Sbjct: 117 -DYQGARDAKSIANFAYKQIKGLLSDRLEGKSKPT-----------GGGSKEKKSEPSAS 164
Query: 502 RNLTWSSFYDLVYDQFEMDLHEVYYAMECNKSKKL 536
L S+F DLV + E+ + E ++A C KKL
Sbjct: 165 VELNASNFDDLVIESNELWIVE-FFAPWCGHCKKL 198
>At1g79540 unknown protein
Length = 780
Score = 30.4 bits (67), Expect = 5.1
Identities = 25/79 (31%), Positives = 32/79 (39%), Gaps = 21/79 (26%)
Query: 530 CNKSKKLKFLKCWMKQVKKSSCHDLNLSEYPKPNQIFAEG-TDSKLNELPQNGEQPIPQV 588
C K K L WMK +KK SC D + + Q F EG T+ L L
Sbjct: 596 CRKRKVLVAFNLWMKYLKKISCLDDETAN--EIEQCFKEGETERALRRL----------- 642
Query: 589 VMSAGINAEVDTKKDDAVL 607
E+DT+KD+ L
Sbjct: 643 -------IELDTRKDELTL 654
>At4g37400 cytochrome P450 monooxygenase -like protein
Length = 501
Score = 30.0 bits (66), Expect = 6.6
Identities = 26/76 (34%), Positives = 35/76 (45%), Gaps = 10/76 (13%)
Query: 55 PLSTSLFAFKFFFSS------LPPHLSFSKLHPFLPKHSFSFDHPFSTFNLLSKTLSTFP 108
PL+ L A+K FF+S LPP +S P L H+ F+ LSKT P
Sbjct: 9 PLALFLLAYKLFFTSKTKRFNLPPSPPYSL--PILGHHNLLKPPVHRLFHRLSKTHG--P 64
Query: 109 NFPLTYNPKAANLIDS 124
F L + + A +I S
Sbjct: 65 IFSLQFGSRRAVVISS 80
>At1g17030 unknown protein
Length = 502
Score = 30.0 bits (66), Expect = 6.6
Identities = 10/21 (47%), Positives = 16/21 (75%)
Query: 751 LLENIQCHMEGGFFGDWNLEN 771
L++N + HM G F+G W+L+N
Sbjct: 199 LVDNEEGHMSGSFYGTWDLDN 219
>At1g22650 invertase like protein
Length = 534
Score = 29.6 bits (65), Expect = 8.6
Identities = 19/67 (28%), Positives = 34/67 (50%), Gaps = 1/67 (1%)
Query: 718 ELQILFRLEILQSEVGRGIEDSSKQKFVKQICLLLENIQCHMEGGFFGDWNLENYVAKII 777
E+Q LF + L+S + D+ ++F+++I L + HM F+ D+ N + +
Sbjct: 259 EIQALFFMA-LRSAMSMLKHDAEGKEFMERIVKRLHALSFHMRSYFWLDFQQLNDIYRYK 317
Query: 778 KSRYSHT 784
YSHT
Sbjct: 318 TEEYSHT 324
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.320 0.137 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,706,224
Number of Sequences: 26719
Number of extensions: 1034279
Number of successful extensions: 2568
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 2545
Number of HSP's gapped (non-prelim): 16
length of query: 972
length of database: 11,318,596
effective HSP length: 109
effective length of query: 863
effective length of database: 8,406,225
effective search space: 7254572175
effective search space used: 7254572175
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 65 (29.6 bits)
Medicago: description of AC121241.8