BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121239.10 - phase: 0
(90 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g22800 alanyl-tRNA synthetase 134 8e-33
At2g17870 putative glycine-rich, zinc-finger DNA-binding protein 32 0.072
At1g49930 hypothetical protein 29 0.36
At1g13020 eukaryotic initiation factor 4B (EIF4B5) 28 0.61
At5g23170 serine/threonine protein kinase-like protein 27 1.4
At3g29080 hypothetical protein 27 1.4
At4g22020 glycine-rich protein 27 1.8
At4g11660 heat shock transcription factor - like protein 27 2.3
At1g24490 hypothetical protein 27 2.3
At1g15830 hypothetical protein 27 2.3
At2g28670 putative disease resistance response protein 26 3.0
At1g07180 unknown protein 26 3.0
At5g07540 glycine-rich protein atGRP-6 26 3.9
At4g26620 sucrase like protein 26 3.9
At3g55430 beta-1,3-glucanase - like protein 26 3.9
At3g46740 chloroplast import-associated channel protein homolog 26 3.9
At3g24410 hypothetical protein 26 3.9
At3g05220 unknown protein 26 3.9
At2g29990 putative NADH dehydrogenase (ubiquinone oxidoreductase) 26 3.9
At1g33240 DNA-binding factor, putative 26 3.9
>At5g22800 alanyl-tRNA synthetase
Length = 954
Score = 134 bits (337), Expect = 8e-33
Identities = 65/88 (73%), Positives = 74/88 (83%)
Query: 1 METLADPAAIVLGSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFA 60
+ TL DP A+VLGS P + KVSLVAAF+PGVV G+QAGKFIG IAKLCGGGGGG+PNFA
Sbjct: 865 ISTLEDPVAVVLGSSPEKDKVSLVAAFSPGVVSLGVQAGKFIGPIAKLCGGGGGGKPNFA 924
Query: 61 QAGGRKPENLAGALEKAKSELIAYLCEK 88
QAGGRKPENL ALEKA+ +L+A L EK
Sbjct: 925 QAGGRKPENLPSALEKAREDLVATLSEK 952
>At2g17870 putative glycine-rich, zinc-finger DNA-binding protein
Length = 301
Score = 31.6 bits (70), Expect = 0.072
Identities = 18/53 (33%), Positives = 28/53 (51%), Gaps = 2/53 (3%)
Query: 14 SCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRK 66
+ PG G ++ + G G G+ +G +AK C GG GG+ +F GGR+
Sbjct: 75 TAPGGGSLNKKENSSRGSGGNCFNCGE-VGHMAKDCDGGSGGK-SFGGGGGRR 125
Score = 26.6 bits (57), Expect = 2.3
Identities = 13/28 (46%), Positives = 16/28 (56%), Gaps = 6/28 (21%)
Query: 42 IGQIAKLC------GGGGGGRPNFAQAG 63
+G IAK+C GGGGGGR + G
Sbjct: 232 VGHIAKVCTSKIPSGGGGGGRACYECGG 259
Score = 25.0 bits (53), Expect = 6.7
Identities = 11/24 (45%), Positives = 14/24 (57%), Gaps = 1/24 (4%)
Query: 42 IGQIAKLCGGGGGGRPNFAQAGGR 65
+G +AK C GG GG + GGR
Sbjct: 169 VGHLAKDCRGGSGGN-RYGGGGGR 191
>At1g49930 hypothetical protein
Length = 134
Score = 29.3 bits (64), Expect = 0.36
Identities = 18/56 (32%), Positives = 22/56 (39%), Gaps = 27/56 (48%)
Query: 1 METLADPAAIVLGSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGR 56
+ TL DP +VLGS P + KLCGGGGGG+
Sbjct: 17 ISTLDDPVVVVLGSSPEKD---------------------------KLCGGGGGGK 45
>At1g13020 eukaryotic initiation factor 4B (EIF4B5)
Length = 549
Score = 28.5 bits (62), Expect = 0.61
Identities = 19/61 (31%), Positives = 25/61 (40%), Gaps = 6/61 (9%)
Query: 17 GEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKPENLAGALEK 76
G+GK SL + QG Q ++ G GGGGGG ++ G G K
Sbjct: 167 GKGKKSLPS------FDQGRQGSRYGGGGGSFGGGGGGGAGSYGGGGAGAGSGGGGGFSK 220
Query: 77 A 77
A
Sbjct: 221 A 221
>At5g23170 serine/threonine protein kinase-like protein
Length = 341
Score = 27.3 bits (59), Expect = 1.4
Identities = 9/13 (69%), Positives = 12/13 (92%)
Query: 43 GQIAKLCGGGGGG 55
G+I ++CGGGGGG
Sbjct: 248 GRIGEICGGGGGG 260
>At3g29080 hypothetical protein
Length = 445
Score = 27.3 bits (59), Expect = 1.4
Identities = 17/48 (35%), Positives = 24/48 (49%), Gaps = 8/48 (16%)
Query: 4 LADPAAIVLGSCPGEGKVSLVAAF----TPGVVG----QGIQAGKFIG 43
L + A++ G CP EG + A+ PGV+G +GI A IG
Sbjct: 243 LLEEGALIRGDCPAEGAAAAAASVDEVGPPGVIGGEGLEGIGAEGNIG 290
>At4g22020 glycine-rich protein
Length = 396
Score = 26.9 bits (58), Expect = 1.8
Identities = 13/26 (50%), Positives = 14/26 (53%)
Query: 39 GKFIGQIAKLCGGGGGGRPNFAQAGG 64
G F G+ AK GGGGGG GG
Sbjct: 48 GSFYGKGAKRYGGGGGGGGGGGGGGG 73
Score = 26.2 bits (56), Expect = 3.0
Identities = 13/32 (40%), Positives = 15/32 (46%)
Query: 33 GQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGG 64
G G AG +G GGGGGG + GG
Sbjct: 120 GSGSGAGAGVGGTTGGVGGGGGGGGGGGEGGG 151
Score = 25.4 bits (54), Expect = 5.1
Identities = 12/23 (52%), Positives = 13/23 (56%)
Query: 33 GQGIQAGKFIGQIAKLCGGGGGG 55
G G AG +G A GGGGGG
Sbjct: 198 GSGAGAGAGVGGAAGGVGGGGGG 220
>At4g11660 heat shock transcription factor - like protein
Length = 377
Score = 26.6 bits (57), Expect = 2.3
Identities = 20/51 (39%), Positives = 21/51 (40%), Gaps = 4/51 (7%)
Query: 16 PGE--GKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGG 64
PGE G+ VA G G G AG G GGGGGG GG
Sbjct: 2 PGEQTGETPTVAGVGGG--GAGCSAGNSGGSSGCGAGGGGGGSGGGGGGGG 50
>At1g24490 hypothetical protein
Length = 593
Score = 26.6 bits (57), Expect = 2.3
Identities = 12/31 (38%), Positives = 16/31 (50%)
Query: 51 GGGGGRPNFAQAGGRKPENLAGALEKAKSEL 81
GGGGG P+ GG+ + EK+ S L
Sbjct: 429 GGGGGSPSKGGGGGKSDSSGKSGEEKSSSNL 459
>At1g15830 hypothetical protein
Length = 483
Score = 26.6 bits (57), Expect = 2.3
Identities = 20/65 (30%), Positives = 27/65 (40%), Gaps = 8/65 (12%)
Query: 8 AAIVLGSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKP 67
A V+ C G G + G G G A + Q+ + CGGGGGG GG +
Sbjct: 362 ATQVMQGCGG-GDAGAITQVMQGWGGGGAGA---VTQVMQGCGGGGGG----GDGGGGQG 413
Query: 68 ENLAG 72
+ G
Sbjct: 414 TGIGG 418
>At2g28670 putative disease resistance response protein
Length = 447
Score = 26.2 bits (56), Expect = 3.0
Identities = 23/74 (31%), Positives = 27/74 (36%), Gaps = 4/74 (5%)
Query: 12 LGSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIA----KLCGGGGGGRPNFAQAGGRKP 67
LG PG G A +G G AG +G A L GGG G P G
Sbjct: 133 LGGGPGAGSALGGGAGAGPALGGGAGAGPALGGGAGAGSALGGGGAGAGPALGGGGAGAG 192
Query: 68 ENLAGALEKAKSEL 81
L G + + S L
Sbjct: 193 PALGGGVAGSGSAL 206
Score = 25.8 bits (55), Expect = 3.9
Identities = 19/61 (31%), Positives = 22/61 (35%), Gaps = 7/61 (11%)
Query: 13 GSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKPENLAG 72
GS P G + A T +G G AG + GGG G P G P G
Sbjct: 114 GSLPTTGSATGAGAGTGSALGGGPGAGSAL-------GGGAGAGPALGGGAGAGPALGGG 166
Query: 73 A 73
A
Sbjct: 167 A 167
>At1g07180 unknown protein
Length = 510
Score = 26.2 bits (56), Expect = 3.0
Identities = 16/54 (29%), Positives = 25/54 (45%), Gaps = 9/54 (16%)
Query: 12 LGSCPG----EGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFAQ 61
+G C G GK +L P + + GK++ + + G GGGR N A+
Sbjct: 380 IGDCSGYLESTGKSTL-----PALAQVAEREGKYLANLFNVMGKAGGGRANSAK 428
>At5g07540 glycine-rich protein atGRP-6
Length = 244
Score = 25.8 bits (55), Expect = 3.9
Identities = 16/52 (30%), Positives = 18/52 (33%)
Query: 13 GSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGG 64
G+ G K + PG G G G GG GG P A GG
Sbjct: 136 GASGGGDKPGGASGGGPGGASGGASGGASGGASGGASGGASGGGPGGASGGG 187
Score = 25.0 bits (53), Expect = 6.7
Identities = 16/46 (34%), Positives = 20/46 (42%), Gaps = 7/46 (15%)
Query: 29 PGVVG--QGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKPENLAG 72
PG G G + G+ G GG G +P A GG KP +G
Sbjct: 109 PGASGGASGDKPGEMSG-----AGGPSGDKPGGASGGGDKPGGASG 149
>At4g26620 sucrase like protein
Length = 443
Score = 25.8 bits (55), Expect = 3.9
Identities = 20/56 (35%), Positives = 28/56 (49%), Gaps = 8/56 (14%)
Query: 37 QAGKFIGQIAKLCGGGGGGRPNFAQAGGRKP----ENLAGALEKAKSELIAYLCEK 88
++G F Q L GGGGG N A G +P E LAG ++ + + +LC K
Sbjct: 40 RSGSF--QSESLLGGGGGESINDADFGFARPDFRSEQLAGTVQFYERHV--FLCYK 91
>At3g55430 beta-1,3-glucanase - like protein
Length = 449
Score = 25.8 bits (55), Expect = 3.9
Identities = 15/43 (34%), Positives = 22/43 (50%), Gaps = 3/43 (6%)
Query: 50 GGGGGGRPNF---AQAGGRKPENLAGALEKAKSELIAYLCEKG 89
G GGGGRP + AGG+ +GA + I ++C +G
Sbjct: 347 GQGGGGRPALPAPSTAGGKWCVARSGATNTQLQDSINWVCGQG 389
>At3g46740 chloroplast import-associated channel protein homolog
Length = 818
Score = 25.8 bits (55), Expect = 3.9
Identities = 11/17 (64%), Positives = 11/17 (64%)
Query: 48 LCGGGGGGRPNFAQAGG 64
L GGGGGG NF GG
Sbjct: 100 LTGGGGGGDGNFGGFGG 116
>At3g24410 hypothetical protein
Length = 240
Score = 25.8 bits (55), Expect = 3.9
Identities = 14/32 (43%), Positives = 18/32 (55%), Gaps = 4/32 (12%)
Query: 33 GQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGG 64
G+G++ G IG + GGGGGGR GG
Sbjct: 192 GEGVEGG--IG--GTVVGGGGGGRAGGRAVGG 219
Score = 24.6 bits (52), Expect = 8.8
Identities = 16/48 (33%), Positives = 19/48 (39%), Gaps = 7/48 (14%)
Query: 17 GEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGG 64
GEG + G G G G+ +G GGGGGR GG
Sbjct: 192 GEGVEGGIGGTVVGGGGGGRAGGRAVG-------GGGGGRAGGRAVGG 232
>At3g05220 unknown protein
Length = 541
Score = 25.8 bits (55), Expect = 3.9
Identities = 16/55 (29%), Positives = 28/55 (50%), Gaps = 7/55 (12%)
Query: 18 EGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKPENLAG 72
+G+V++ P ++ + + G+ A++ GGGGGG GG+ NL G
Sbjct: 10 QGRVTVTGNIDPALLVKKLSKS---GKHAEILGGGGGG----GGGGGKGFPNLNG 57
>At2g29990 putative NADH dehydrogenase (ubiquinone oxidoreductase)
Length = 508
Score = 25.8 bits (55), Expect = 3.9
Identities = 16/54 (29%), Positives = 24/54 (43%), Gaps = 9/54 (16%)
Query: 12 LGSCPG----EGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFAQ 61
+G C G GK +L P + + GK++ + G G GGR N A+
Sbjct: 378 IGDCSGYLETTGKPTL-----PALAQVAEREGKYLANLLNAIGKGNGGRANSAK 426
>At1g33240 DNA-binding factor, putative
Length = 338
Score = 25.8 bits (55), Expect = 3.9
Identities = 12/32 (37%), Positives = 18/32 (55%)
Query: 50 GGGGGGRPNFAQAGGRKPENLAGALEKAKSEL 81
GGGGGG + +G R P AL + +S++
Sbjct: 47 GGGGGGGSASSSSGNRWPREETLALLRIRSDM 78
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.315 0.139 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,100,758
Number of Sequences: 26719
Number of extensions: 88700
Number of successful extensions: 470
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 406
Number of HSP's gapped (non-prelim): 70
length of query: 90
length of database: 11,318,596
effective HSP length: 66
effective length of query: 24
effective length of database: 9,555,142
effective search space: 229323408
effective search space used: 229323408
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC121239.10