BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121233.6 - phase: 2
(123 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g17850 putative senescence-associated rhodanese protein 150 1e-37
At5g66170 senescence-associated protein sen1-like protein 146 3e-36
At5g66040 senescence-associated protein sen1-like protein; ketoc... 99 4e-22
At4g35770 senescence-associated protein sen1 99 5e-22
At4g27700 unknown protein 51 2e-07
At3g08920 unknown protein 38 0.001
At2g42220 unknown protein 38 0.001
At5g19370 peptidyl-prolyl cis-trans isomerase - like protein 35 0.009
At1g17850 hypothetical protein 30 0.23
At5g14230 ankyrin - like protein 27 2.5
At1g66235 putative protein 27 3.3
At1g63020 RNA polymerase IIA largest subunit, putative 27 3.3
At3g04240 O-linked GlcNAc transferase like protein 26 4.3
At5g08100 asparaginase 26 5.6
At2g21040 hypothetical protein 26 5.6
At1g43860 unknown protein 26 5.6
At5g29100 putative protein 25 7.3
At3g29360 UDP-glucose dehydrogenase, putative 25 7.3
At2g40755 rhodanese like protein 25 7.3
At2g27170 putative chromosome associated protein 25 7.3
>At2g17850 putative senescence-associated rhodanese protein
Length = 150
Score = 150 bits (379), Expect = 1e-37
Identities = 69/123 (56%), Positives = 92/123 (74%)
Query: 1 SKTEVVTVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPE 60
S+ +V+T+DV + L+ + + +LDVRTVEEF+KGHVDSE + N+ Y TP+G+ NP
Sbjct: 2 SEPKVITIDVNQAQKLLDSGYTFLDVRTVEEFKKGHVDSENVFNVPYWLYTPQGQEINPN 61
Query: 61 FLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFPVKIH 120
FLK VSSLCN+ DHLI+GC+SGVRS++AT L++ GFK V NM GGY+ WV K FPVK+
Sbjct: 62 FLKHVSSLCNQTDHLILGCKSGVRSLHATKFLVSSGFKTVRNMDGGYIAWVNKRFPVKVE 121
Query: 121 VTE 123
E
Sbjct: 122 HKE 124
>At5g66170 senescence-associated protein sen1-like protein
Length = 136
Score = 146 bits (368), Expect = 3e-36
Identities = 68/120 (56%), Positives = 90/120 (74%)
Query: 4 EVVTVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPEFLK 63
EVV+VDV K+L+++ H YLDVRT +EF++GH ++ KI+NI YM NTP+GRVKN EFL+
Sbjct: 13 EVVSVDVSQAKTLLQSGHQYLDVRTQDEFRRGHCEAAKIVNIPYMLNTPQGRVKNQEFLE 72
Query: 64 EVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFPVKIHVTE 123
+VSSL N D ++VGCQSG RS+ AT +L+A G+K V N+GGGYL WV FP+ E
Sbjct: 73 QVSSLLNPADDILVGCQSGARSLKATTELVAAGYKKVRNVGGGYLAWVDHSFPINTEEEE 132
>At5g66040 senescence-associated protein sen1-like protein;
ketoconazole resistance protein-like
Length = 120
Score = 99.4 bits (246), Expect = 4e-22
Identities = 53/112 (47%), Positives = 66/112 (58%), Gaps = 2/112 (1%)
Query: 7 TVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPEFLKEVS 66
+V V L+ H YLDVRT EEF +GH IN+ YM G KNP+FL++VS
Sbjct: 10 SVSVTVAHDLLLAGHRYLDVRTPEEFSQGHACGA--INVPYMNRGASGMSKNPDFLEQVS 67
Query: 67 SLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFPVK 118
S + D++IVGCQSG RS+ AT DLL GF V ++ GGY W K P K
Sbjct: 68 SHFGQSDNIIVGCQSGGRSIKATTDLLHAGFTGVKDIVGGYSAWAKNGLPTK 119
>At4g35770 senescence-associated protein sen1
Length = 182
Score = 99.0 bits (245), Expect = 5e-22
Identities = 48/112 (42%), Positives = 68/112 (59%), Gaps = 2/112 (1%)
Query: 7 TVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPEFLKEVS 66
+V V + L + + YLDVRT +EF GH + IN+ YM+ G VKNP FL++VS
Sbjct: 72 SVPVRVARELAQAGYRYLDVRTPDEFSIGH--PTRAINVPYMYRVGSGMVKNPSFLRQVS 129
Query: 67 SLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFPVK 118
S K D +I+GC+SG S A+ DLL GF + ++ GGY+ W + E PV+
Sbjct: 130 SHFRKHDEIIIGCESGQMSFMASTDLLTAGFTAITDIAGGYVAWTENELPVE 181
>At4g27700 unknown protein
Length = 224
Score = 50.8 bits (120), Expect = 2e-07
Identities = 42/147 (28%), Positives = 65/147 (43%), Gaps = 34/147 (23%)
Query: 5 VVTVDVLATKSLIKTTH-VYLDVRTVEEFQKGHV------------------DSEKIINI 45
V +VDV + L K + V LDVR E++ GH D + +
Sbjct: 74 VRSVDVKEAQRLQKENNFVILDVRPEAEYKAGHPPGAINVEMYRLIREWTAWDIARRLGF 133
Query: 46 AYMFNTPEGRVKNPEFLKEVSSLCNKEDHLIVGCQSG--------------VRSVYATAD 91
A+ F G +NPEF++ V + +KE +IV C S RS+ A
Sbjct: 134 AF-FGIFSGTEENPEFIQSVEAKLDKEAKIIVACSSAGTMKPTQNLPEGQQSRSLIAAYL 192
Query: 92 LLAEGFKDVYNMGGGYLEWVKKEFPVK 118
L+ G+K+V+++ GG W K+ PV+
Sbjct: 193 LVLNGYKNVFHLEGGIYTWGKEGLPVE 219
>At3g08920 unknown protein
Length = 214
Score = 37.7 bits (86), Expect = 0.001
Identities = 21/60 (35%), Positives = 36/60 (60%), Gaps = 1/60 (1%)
Query: 58 NPEF-LKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFP 116
N EF L+ V ++ +KE ++V C G+RS+ A + L EG+K + + GG+ + +FP
Sbjct: 120 NDEFALRVVEAVPDKESKVLVVCGEGLRSLAAVSKLHGEGYKSLGWLTGGFNRVSEGDFP 179
>At2g42220 unknown protein
Length = 234
Score = 37.7 bits (86), Expect = 0.001
Identities = 31/120 (25%), Positives = 54/120 (44%), Gaps = 24/120 (20%)
Query: 4 EVVTVDVLATKSLI-KTTHVYLDVRTVEEFQKGHVDSEKIINI----------------- 45
E+ V+ K LI + + +DVR +F++ H+ S I +
Sbjct: 48 ELKFVNAEEAKQLIAEEGYSVVDVRDKTQFERAHIKSCSHIPLFIYNEDNDIGTIIKRTV 107
Query: 46 -----AYMFNTPEGRVKNPEFLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDV 100
F P +V NPEFLK V + +++ L++ CQ G+RS A + L G++++
Sbjct: 108 HNNFSGLFFGLPFTKV-NPEFLKSVRNEFSQDSKLLLVCQEGLRSAAAASRLEEAGYENI 166
>At5g19370 peptidyl-prolyl cis-trans isomerase - like protein
Length = 299
Score = 35.0 bits (79), Expect = 0.009
Identities = 19/53 (35%), Positives = 28/53 (51%)
Query: 64 EVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFP 116
+++S N E V C+ G RS+ L ++GFK VYN+ GG + K P
Sbjct: 242 DITSKLNPEKDTFVLCKVGGRSMQVANWLQSQGFKSVYNITGGIQAYSLKVDP 294
>At1g17850 hypothetical protein
Length = 366
Score = 30.4 bits (67), Expect = 0.23
Identities = 15/41 (36%), Positives = 26/41 (62%), Gaps = 2/41 (4%)
Query: 75 LIVGCQSGVR-SVYATADLLAEGFKDVYNMGGGYLEWVKKE 114
+++ C G+R VY+T L GFK++Y + GG ++K+E
Sbjct: 247 ILMYCTGGIRCDVYSTV-LRQRGFKNLYTLKGGVSHYLKEE 286
>At5g14230 ankyrin - like protein
Length = 591
Score = 26.9 bits (58), Expect = 2.5
Identities = 28/107 (26%), Positives = 43/107 (40%), Gaps = 23/107 (21%)
Query: 9 DVLATKSLIKTTHVYLDVRTVEEF-------QKGHVDSEKIINIAYMFNTPEGRVKNPEF 61
D A K+L+K +YLD + E F GHV++ +++ A
Sbjct: 296 DAEALKALVKAQDIYLDYQDEEGFSAAMLAAMNGHVEAFRVLVYAG-------------- 341
Query: 62 LKEVSSLCNKEDHLI-VGCQSGVRSVYATADLLAEGFKDVYNMGGGY 107
+V N D ++ + Q+G R V L KD NM GG+
Sbjct: 342 -ADVKLYNNSGDTVVSLSEQNGNRDVIEKVMLEFALEKDSRNMAGGF 387
>At1g66235 putative protein
Length = 265
Score = 26.6 bits (57), Expect = 3.3
Identities = 12/37 (32%), Positives = 22/37 (59%)
Query: 14 KSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFN 50
KS K HV+ +R E+F+ G ++K++N+ + N
Sbjct: 91 KSGWKFDHVWNIIRNFEKFKDGATQAKKVLNLCGLEN 127
>At1g63020 RNA polymerase IIA largest subunit, putative
Length = 1453
Score = 26.6 bits (57), Expect = 3.3
Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 10/39 (25%)
Query: 42 IINIAYMFNTPEGRVKNPEFLKEVSSLCNKEDHLIVGCQ 80
+IN AY + NP FLKEV++L NK + GC+
Sbjct: 73 VINFAYS-------IINPYFLKEVAALLNK---ICPGCK 101
>At3g04240 O-linked GlcNAc transferase like protein
Length = 977
Score = 26.2 bits (56), Expect = 4.3
Identities = 13/48 (27%), Positives = 25/48 (52%), Gaps = 4/48 (8%)
Query: 19 TTHVYLDVRTVEEF----QKGHVDSEKIINIAYMFNTPEGRVKNPEFL 62
T Y+D +EF Q H+ SEK++++ + + + + KN + L
Sbjct: 704 TGATYIDYLVTDEFVSPLQYAHIYSEKLVHLPHCYFVNDYKQKNQDVL 751
>At5g08100 asparaginase
Length = 315
Score = 25.8 bits (55), Expect = 5.6
Identities = 21/58 (36%), Positives = 25/58 (42%), Gaps = 7/58 (12%)
Query: 3 TEVVTVDVLATKSLIKTTHVYLDVRTVEEFQKGH----VDSEKII---NIAYMFNTPE 53
T VV LA + KT H+YL E F + H VDS I NIA + E
Sbjct: 100 TTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKE 157
>At2g21040 hypothetical protein
Length = 277
Score = 25.8 bits (55), Expect = 5.6
Identities = 12/25 (48%), Positives = 14/25 (56%)
Query: 1 SKTEVVTVDVLATKSLIKTTHVYLD 25
S +V TVDV K + T H YLD
Sbjct: 184 SVEDVETVDVYTAKGFLSTGHRYLD 208
>At1g43860 unknown protein
Length = 370
Score = 25.8 bits (55), Expect = 5.6
Identities = 13/39 (33%), Positives = 20/39 (50%)
Query: 56 VKNPEFLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLA 94
V E ++S++C E L C S VRS+ ++LA
Sbjct: 200 VSKDESGTQMSTVCEMEPGLFRECDSHVRSIQGRLEILA 238
>At5g29100 putative protein
Length = 481
Score = 25.4 bits (54), Expect = 7.3
Identities = 17/48 (35%), Positives = 22/48 (45%)
Query: 29 VEEFQKGHVDSEKIINIAYMFNTPEGRVKNPEFLKEVSSLCNKEDHLI 76
VEE Q +VD + I + T E K E L + L K D+LI
Sbjct: 143 VEENQVKNVDDQSRIQASLRIVTMESSRKYVELLDRILDLDQKVDNLI 190
>At3g29360 UDP-glucose dehydrogenase, putative
Length = 480
Score = 25.4 bits (54), Expect = 7.3
Identities = 19/59 (32%), Positives = 25/59 (42%), Gaps = 15/59 (25%)
Query: 56 VKNPEFLKE---VSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWV 111
+ NPEFL E + L N + LI G ++ EGFK V + Y WV
Sbjct: 153 LSNPEFLAEGTAIKDLFNPDRVLIGGRET------------PEGFKAVQTLKNVYAHWV 199
>At2g40755 rhodanese like protein
Length = 426
Score = 25.4 bits (54), Expect = 7.3
Identities = 20/73 (27%), Positives = 35/73 (47%), Gaps = 20/73 (27%)
Query: 50 NTPEGRVKNPEFLKEVSSLCNKED---------HLIVGCQSGVRSVYATADLLAEGFKDV 100
N + +V+ +F S + +KED + + C G+R A++ LL++GF++
Sbjct: 256 NETQAKVEKEDF----SEITHKEDKAEKPKTLPRIAMYCTGGIRCEKASSLLLSQGFEE- 310
Query: 101 YNMGGGYLEWVKK 113
YLE V K
Sbjct: 311 ------YLEEVPK 317
>At2g27170 putative chromosome associated protein
Length = 1163
Score = 25.4 bits (54), Expect = 7.3
Identities = 13/37 (35%), Positives = 22/37 (59%)
Query: 4 EVVTVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSE 40
E TV+ TK+L K T + LDV+ ++ G++ S+
Sbjct: 287 EKETVEAQQTKALKKKTKLELDVKDFQDRITGNIQSK 323
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.135 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,815,762
Number of Sequences: 26719
Number of extensions: 111207
Number of successful extensions: 277
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 260
Number of HSP's gapped (non-prelim): 22
length of query: 123
length of database: 11,318,596
effective HSP length: 87
effective length of query: 36
effective length of database: 8,994,043
effective search space: 323785548
effective search space used: 323785548
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)
Medicago: description of AC121233.6