BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149582.7 - phase: 0
(155 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW559955 similar to GP|21593487|gb| unknown {Arabidopsis thalian... 114 2e-26
TC77445 homologue to GP|19911575|dbj|BAB86891. syringolide-induc... 64 2e-11
TC89113 homologue to GP|19911575|dbj|BAB86891. syringolide-induc... 64 2e-11
TC86353 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17 ... 39 8e-04
TC78696 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17 ... 36 0.005
BQ137242 similar to GP|9800281|gb|A pR69 {rat cytomegalovirus Ma... 31 0.18
TC83532 similar to PIR|T38639|T38639 proteasome component PUP2 h... 30 0.51
TC84067 similar to GP|19698921|gb|AAL91196.1 protein kinase put... 28 1.5
TC79846 similar to GP|8843728|dbj|BAA97276.1 homeodomain transcr... 28 1.9
TC87629 similar to GP|17381280|gb|AAL36058.1 At2g22250/T26C19.9 ... 28 1.9
TC83392 similar to PIR|T51833|T51833 transcription factor DREB2A... 28 1.9
TC88684 similar to GP|7576200|emb|CAB87861.1 protein synthesis i... 27 2.5
TC78038 weakly similar to GP|21592898|gb|AAM64848.1 unknown {Ara... 27 3.3
TC76847 homologue to SP|P08927|RUBB_PEA RuBisCO subunit binding-... 27 4.3
TC78861 similar to SP|P57741|PFD3_ARATH Probable prefoldin subun... 27 4.3
TC84558 weakly similar to SP|Q04634|EF1A_TETPY Elongation factor... 27 4.3
BE941998 similar to GP|6634762|gb| Contains a bZIP transcription... 27 4.3
TC89252 27 4.3
TC89693 weakly similar to GP|21104633|dbj|BAB93225. hypothetical... 26 5.6
AJ388878 26 7.4
>AW559955 similar to GP|21593487|gb| unknown {Arabidopsis thaliana}, partial
(7%)
Length = 411
Score = 114 bits (285), Expect = 2e-26
Identities = 73/140 (52%), Positives = 91/140 (64%), Gaps = 6/140 (4%)
Frame = +1
Query: 22 SVSSYQNPTLSPNASVTTARKVSIIPKEARRKH-RSISFSAREPSSPKVSCMGQVKS-KK 79
S+ YQNPTLSP S TT V + PKEARRKH R ISFS +EP+SPKVSCMG++KS KK
Sbjct: 4 SLVGYQNPTLSPCRSPTT--HVLLFPKEARRKHKRGISFSPKEPTSPKVSCMGEIKSKKK 177
Query: 80 KKRKAKRIHQSSTKNNDSVTSHENKKILLQSEKALVVEENQ----EFAPSMLIVPSLGTM 135
KK+K K++H K +V NK +LL K Q + + + + P+L +M
Sbjct: 178 KKKKEKKLH----KGIQNVV--PNKNVLLWISKGSYEGGRQGGKEKESAIVSVPPTLNSM 339
Query: 136 KKFESGRGSLSDFDATLAER 155
KKF SGRGSL DFDATL+ER
Sbjct: 340 KKFTSGRGSLCDFDATLSER 399
>TC77445 homologue to GP|19911575|dbj|BAB86891. syringolide-induced protein
14-1-1 {Glycine max}, partial (22%)
Length = 978
Score = 63.9 bits (154), Expect = 2e-11
Identities = 55/175 (31%), Positives = 80/175 (45%), Gaps = 27/175 (15%)
Frame = +2
Query: 2 EKLSKMKNKFFKFLPKRPVASVSSYQNPTLSP---------NASVTTARK---------- 42
+K SK++NKF K A ++QN SP N++ K
Sbjct: 101 DKQSKLRNKFMK-------AITITFQNAPFSPGRDHKFRSENSNTKWGAKTHAVGKGFSG 259
Query: 43 --VSIIPKEARRKHRSISFS-AREPSSPKVSCMGQVKSKKKKRKAKRIHQSSTKNNDS-V 98
V++IP EARRK + + +EP+SPK+SCMGQ+K KKK K T D+ V
Sbjct: 260 PMVTMIPYEARRKPKDGGGNETQEPTSPKISCMGQIKHHKKKNIPKPNKDLFTPKVDTEV 439
Query: 99 TSHENKKILLQSEKALVVEENQEFA----PSMLIVPSLGTMKKFESGRGSLSDFD 149
+ ++I Q KA E + + P +G M++F SGR + S FD
Sbjct: 440 KRSKFQRIFSQRSKAKFAERKSDGSGFVDEGNNKAPPMGDMRRFASGREAFSKFD 604
>TC89113 homologue to GP|19911575|dbj|BAB86891. syringolide-induced protein
14-1-1 {Glycine max}, partial (24%)
Length = 703
Score = 63.9 bits (154), Expect = 2e-11
Identities = 54/179 (30%), Positives = 78/179 (43%), Gaps = 30/179 (16%)
Frame = +2
Query: 1 MEKLSKMKNKFFKFLPKRPVASVSSYQNPTLSPNASVTTARK------------------ 42
MEK K++NK K LPK ++QN SP + +
Sbjct: 143 MEKNLKLRNKILKILPK---TITINFQNHPFSPGRDHKSRQDNATKWARPHGVKGFSGPI 313
Query: 43 VSIIPKEARRKHRSIS-FSAREPSSPKVSCMGQVKSKKKK-RKAKRIHQSSTKNN-DSVT 99
SIIP+EARRK + I+ EP+SPK+SCMGQ+K KKK+ +K+K + N +
Sbjct: 314 QSIIPREARRKSKDINGIDYEEPTSPKISCMGQIKHKKKQIKKSKDMEVKKHVNTFQKML 493
Query: 100 SHENK---------KILLQSEKALVVEENQEFAPSMLIVPSLGTMKKFESGRGSLSDFD 149
H K +K +EE + MK+F SGR + ++FD
Sbjct: 494 FHVGKPKSEGRRKSDACAVQDKKYAIEER---------ATHVSQMKRFASGRDTFANFD 643
>TC86353 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17
{Arabidopsis thaliana}, partial (12%)
Length = 1351
Score = 38.9 bits (89), Expect = 8e-04
Identities = 40/146 (27%), Positives = 54/146 (36%), Gaps = 48/146 (32%)
Frame = +1
Query: 52 RKHRSISFSAREPSSPKVSCMGQVKSKKKK------------------------------ 81
++ S+ S EPSSPKVSCMG+V+SK+ K
Sbjct: 472 KRSASVGKSFVEPSSPKVSCMGKVRSKRGKTSTVTAAAKAAVKEKTARTERKQSKHSFFK 651
Query: 82 -----------RKAKRIHQSS-------TKNNDSVTSHENKKILLQSEKALVVEENQEFA 123
K R++ SS TKN +SV+ + + VE +
Sbjct: 652 SFRAIFRSGGRNKHDRMNDSSALDSSSITKNTNSVSDSKARDSTASLNDVSFVESVTRNS 831
Query: 124 PSMLIVPSLGTMKKFESGRGSLSDFD 149
S P LG M +F SGR S S D
Sbjct: 832 VSEGEPPGLGVMMRFTSGRRSESWVD 909
>TC78696 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17
{Arabidopsis thaliana}, partial (13%)
Length = 1108
Score = 36.2 bits (82), Expect = 0.005
Identities = 16/29 (55%), Positives = 21/29 (72%)
Frame = +3
Query: 63 EPSSPKVSCMGQVKSKKKKRKAKRIHQSS 91
EPSSPKVSC+G+V+SK+ + R Q S
Sbjct: 717 EPSSPKVSCIGRVRSKRGHNRRLRTRQRS 803
>BQ137242 similar to GP|9800281|gb|A pR69 {rat cytomegalovirus Maastricht},
partial (2%)
Length = 1023
Score = 31.2 bits (69), Expect = 0.18
Identities = 14/51 (27%), Positives = 28/51 (54%), Gaps = 3/51 (5%)
Frame = +3
Query: 46 IPKEARRKHRSISFSAREPSSPK---VSCMGQVKSKKKKRKAKRIHQSSTK 93
+P+ + ++ + +EP++PK + + KK K+K K+ HQ +TK
Sbjct: 39 LPRRGSKGNKECPYPVKEPTNPKRRGKRSRTKARKKKGKKKHKKKHQKNTK 191
>TC83532 similar to PIR|T38639|T38639 proteasome component PUP2 homolog -
fission yeast (Schizosaccharomyces pombe), partial (97%)
Length = 901
Score = 29.6 bits (65), Expect = 0.51
Identities = 21/63 (33%), Positives = 28/63 (44%)
Frame = +1
Query: 29 PTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSCMGQVKSKKKKRKAKRIH 88
P SP+ + +T S R S+S SAR P + K SC G + S R R+
Sbjct: 349 PA*SPSPTPSTIMSPSASSLAPRPSATSLSASARVPKARKPSCPGPLASPS*LRATTRMV 528
Query: 89 QSS 91
SS
Sbjct: 529 PSS 537
>TC84067 similar to GP|19698921|gb|AAL91196.1 protein kinase putative
{Arabidopsis thaliana}, partial (4%)
Length = 657
Score = 28.1 bits (61), Expect = 1.5
Identities = 14/61 (22%), Positives = 28/61 (44%)
Frame = -1
Query: 23 VSSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSCMGQVKSKKKKR 82
V++ +NP PN ++ + + + + + F+ P +PK + K KKK +
Sbjct: 255 VNNKKNPQTKPNTAIFLSNSIFFLHTHKPKPQKGSKFAVGSPITPK-----RKKGKKKDK 91
Query: 83 K 83
K
Sbjct: 90 K 88
>TC79846 similar to GP|8843728|dbj|BAA97276.1 homeodomain transcription
factor-like {Arabidopsis thaliana}, partial (42%)
Length = 679
Score = 27.7 bits (60), Expect = 1.9
Identities = 14/51 (27%), Positives = 28/51 (54%)
Frame = +2
Query: 69 VSCMGQVKSKKKKRKAKRIHQSSTKNNDSVTSHENKKILLQSEKALVVEEN 119
+S G+ K K +++++K+ K ++ + NKK L E+ ++EEN
Sbjct: 170 ISQQGEKKPKPRRKRSKK-----NKGGENSANDMNKKRKLSDEQVNILEEN 307
>TC87629 similar to GP|17381280|gb|AAL36058.1 At2g22250/T26C19.9
{Arabidopsis thaliana}, partial (72%)
Length = 1309
Score = 27.7 bits (60), Expect = 1.9
Identities = 15/49 (30%), Positives = 26/49 (52%)
Frame = +2
Query: 22 SVSSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVS 70
++S N L N + T + +P + +HRS+SFS+ + PK+S
Sbjct: 68 NLSDMANTFLHNNTTCTISFPDKSLPAISVSRHRSLSFSSNLSTFPKMS 214
>TC83392 similar to PIR|T51833|T51833 transcription factor DREB2A drought
induced [validated] - Arabidopsis thaliana, partial
(22%)
Length = 742
Score = 27.7 bits (60), Expect = 1.9
Identities = 15/41 (36%), Positives = 21/41 (50%)
Frame = +3
Query: 66 SPKVSCMGQVKSKKKKRKAKRIHQSSTKNNDSVTSHENKKI 106
S +V G V+ K+ K +HQ S KN S T NK++
Sbjct: 603 SKEVMIDGTVQCPTNKKCKKMVHQRSYKNVKSETHGRNKRL 725
>TC88684 similar to GP|7576200|emb|CAB87861.1 protein synthesis initiation
factor-like {Arabidopsis thaliana}, partial (3%)
Length = 1322
Score = 27.3 bits (59), Expect = 2.5
Identities = 22/89 (24%), Positives = 38/89 (41%), Gaps = 2/89 (2%)
Frame = +3
Query: 63 EPSSPKVSCMGQVKSKKKKRKAKRIHQSSTKNNDSVTSHENKKILLQSEKALVVEENQEF 122
EPS K + G+ K K+ +KA +S N ENK+ + SE A V ++
Sbjct: 975 EPSKVKGTSKGKKKRKEILQKADASGSTSDLYNAYKGPEENKEAVATSESAANVSTSENL 1154
Query: 123 APSMLIVPSLGTMKKFES--GRGSLSDFD 149
++ T+ +S + L D++
Sbjct: 1155 KQLLMDAAQPATVANEQSKQSKAELEDWE 1241
>TC78038 weakly similar to GP|21592898|gb|AAM64848.1 unknown {Arabidopsis
thaliana}, partial (60%)
Length = 1986
Score = 26.9 bits (58), Expect = 3.3
Identities = 18/61 (29%), Positives = 32/61 (51%)
Frame = -3
Query: 27 QNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSCMGQVKSKKKKRKAKR 86
Q+P + P ASV A+ +I+P+ A + S S S + + K +KK+R +++
Sbjct: 229 QHP-IPPLASVDEAKTTTILPRRATKPPPSWSGSKIGKDGAACEGVKKEKKRKKRRCSRK 53
Query: 87 I 87
I
Sbjct: 52 I 50
>TC76847 homologue to SP|P08927|RUBB_PEA RuBisCO subunit binding-protein
beta subunit chloroplast precursor (60 kDa chaperonin
beta subunit), partial (96%)
Length = 2096
Score = 26.6 bits (57), Expect = 4.3
Identities = 27/119 (22%), Positives = 55/119 (45%), Gaps = 8/119 (6%)
Frame = +3
Query: 1 MEKLSKMKNKFFKFLPKRPVASVSSYQNPTLSP----NASVTTARKVSIIPKEARRKHRS 56
+E LSK K+ +A++ S + T++ + +++++ +S P +RRK S
Sbjct: 69 LENLSKTKSS*-------TLATIFSQFHTTMASLNLSSCNLSSSASISSFPSSSRRKTTS 227
Query: 57 ISFSAREPSSPKVSCMG-QVKSKKKKRKAKRIHQSSTKNNDSV---TSHENKKILLQSE 111
R+ +PKVS M ++ K K++ K D V + + ++L+S+
Sbjct: 228 NVALTRKNRNPKVSAMAKELYFNKDGSAIKKLQNGVNKLADLVGVTLGPKGRNVVLESK 404
>TC78861 similar to SP|P57741|PFD3_ARATH Probable prefoldin subunit 3.
[Mouse-ear cress] {Arabidopsis thaliana}, partial (90%)
Length = 958
Score = 26.6 bits (57), Expect = 4.3
Identities = 20/65 (30%), Positives = 30/65 (45%)
Frame = -2
Query: 3 KLSKMKNKFFKFLPKRPVASVSSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAR 62
+ SK+ +F +R VAS + Y N T +P+ T S+ EAR S + +
Sbjct: 510 RTSKLALALSRFFCRRAVASSNEYSNITFAPSHKHTE----SVSSMEAREYMPSDTSKSA 343
Query: 63 EPSSP 67
SSP
Sbjct: 342 ISSSP 328
>TC84558 weakly similar to SP|Q04634|EF1A_TETPY Elongation factor 1-alpha
(EF-1-alpha) (14-nm filament-associated protein).
{Tetrahymena pyriformis}, partial (42%)
Length = 611
Score = 26.6 bits (57), Expect = 4.3
Identities = 16/51 (31%), Positives = 26/51 (50%)
Frame = +2
Query: 74 QVKSKKKKRKAKRIHQSSTKNNDSVTSHENKKILLQSEKALVVEENQEFAP 124
++K+K KR K + + N + + + I+L K +VVE Q FAP
Sbjct: 353 EIKTKLDKRTGKVVEE----NPAHIKTGDAAMIVLTPTKPMVVEVYQNFAP 493
>BE941998 similar to GP|6634762|gb| Contains a bZIP transcription factor
PF|00170 domain. ESTs gb|R30400 gb|AA650964
gb|AI994521, partial (15%)
Length = 640
Score = 26.6 bits (57), Expect = 4.3
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = -1
Query: 69 VSCMGQVKSKKKKRKAKRIHQSSTKNND 96
V C G K KKK++K K S T NND
Sbjct: 619 VQC-GTKKEKKKRKKTKATVYSQTLNND 539
>TC89252
Length = 646
Score = 26.6 bits (57), Expect = 4.3
Identities = 13/32 (40%), Positives = 21/32 (65%), Gaps = 4/32 (12%)
Frame = -2
Query: 74 QVKSKKKKRKAKRI----HQSSTKNNDSVTSH 101
++ S ++K + K+I HQSS K N+ +TSH
Sbjct: 162 RIPSLQEKTRCKKIIFFLHQSSEKKNNFLTSH 67
>TC89693 weakly similar to GP|21104633|dbj|BAB93225. hypothetical
protein~similar to Arabidopsis thaliana chromosome 3
At3g46550, partial (53%)
Length = 1346
Score = 26.2 bits (56), Expect = 5.6
Identities = 17/48 (35%), Positives = 22/48 (45%)
Frame = +1
Query: 96 DSVTSHENKKILLQSEKALVVEENQEFAPSMLIVPSLGTMKKFESGRG 143
D + S ILL K L+ N A SML S+ +FE+G G
Sbjct: 577 DLMASETRPSILLNITKTLIDAHNFNVAASMLSASSVA--NEFEAGEG 714
>AJ388878
Length = 436
Score = 25.8 bits (55), Expect = 7.4
Identities = 13/44 (29%), Positives = 23/44 (51%)
Frame = -2
Query: 22 SVSSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPS 65
S + PT + ++ + R++S P + +HR+ S REPS
Sbjct: 366 SPTRVHEPTYN*SSGIHRIRRLSFEPYKLLHRHRNESCCDREPS 235
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.310 0.123 0.323
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,792,749
Number of Sequences: 36976
Number of extensions: 45200
Number of successful extensions: 355
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 349
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 349
length of query: 155
length of database: 9,014,727
effective HSP length: 88
effective length of query: 67
effective length of database: 5,760,839
effective search space: 385976213
effective search space used: 385976213
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC149582.7