BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149471.8 + phase: 0
(229 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC81324 similar to GP|21553871|gb|AAM62964.1 unknown {Arabidopsi... 258 1e-69
TC91951 similar to GP|5441893|dbj|BAA82391.1 ESTs C99174(E10437)... 131 2e-31
BE998796 similar to GP|5441893|dbj| ESTs C99174(E10437) D22295(C... 131 2e-31
TC91486 similar to GP|5441893|dbj|BAA82391.1 ESTs C99174(E10437)... 114 4e-26
TC84988 similar to GP|14018368|emb|CAC38358. zinc finger protein... 48 4e-06
BI265088 36 0.014
BF635696 36 0.014
TC87072 similar to PIR|D84581|D84581 probable CCCH-type zinc fin... 35 0.018
TC92272 35 0.031
TC77687 similar to PIR|D84581|D84581 probable CCCH-type zinc fin... 35 0.031
BG646281 weakly similar to PIR|E84465|E844 hypothetical protein ... 33 0.091
TC92368 weakly similar to GP|19347974|gb|AAL86319.1 unknown prot... 32 0.15
TC76784 similar to PIR|G84825|G84825 probable CCCH-type zinc fin... 32 0.15
BI271089 similar to GP|9294325|dbj| gene_id:K24M9.13~unknown pro... 32 0.26
TC91115 similar to PIR|D96705|D96705 probable zinc finger protei... 31 0.34
TC80592 similar to GP|19310435|gb|AAL84954.1 F17H15.1/F17H15.1 {... 31 0.34
TC77987 weakly similar to GP|17979277|gb|AAL49864.1 unknown prot... 31 0.45
AJ501021 similar to PIR|C84587|C84 hypothetical protein At2g2028... 31 0.45
TC82778 similar to PIR|T04011|T04011 hypothetical protein T5L19.... 30 0.59
TC86255 similar to PIR|D84581|D84581 probable CCCH-type zinc fin... 30 0.77
>TC81324 similar to GP|21553871|gb|AAM62964.1 unknown {Arabidopsis
thaliana}, partial (75%)
Length = 1156
Score = 258 bits (659), Expect = 1e-69
Identities = 136/234 (58%), Positives = 163/234 (69%), Gaps = 14/234 (5%)
Frame = +1
Query: 7 SPAIPPIGRNPNVPQSF--PDGSSPPVAKTRLCNKFNTAEGCKFGDKCHFAHGEWELGRP 64
+PA PP RN P++ P+GS+P K+R+C+KFNTAEGCKFGDKCHFAHGEWELG+P
Sbjct: 313 TPAAPPAPRNVPAPRNAHAPNGSAPSAVKSRICSKFNTAEGCKFGDKCHFAHGEWELGKP 492
Query: 65 TVPAYE-----DTRAMGQMQSSSVGG-RIEPPPPAHGAAAGFGVSATATVSINATLAGAI 118
P+++ D R MG + GG R+EPPP A FG +ATA +S+ A+LAGAI
Sbjct: 493 VAPSFDDHRHNDHRHMGPPNAGRFGGHRMEPPP----VPASFGANATAKISVEASLAGAI 660
Query: 119 IGKNDVNSKQICHITGAKLSIREHDSDPNLRNIELEGSFDQIKQASAMVHDLILNV---- 174
IGK VNSKQIC TGAKL+IREH+ DPNL+NIEL G+F+QIK AS MV DL+L +
Sbjct: 661 IGKGGVNSKQICRQTGAKLAIREHE-DPNLKNIELVGTFEQIKDASNMVKDLLLTLQMSA 837
Query: 175 --SSVSGPPGKNITSQTSAPANNFKTKLCENFTKGSCTFGERCHFAHGTDELRK 226
S GPPG + NN KTKLCENF KGSCTFG+RCHFAHG ELRK
Sbjct: 838 PPKSNQGPPGHH--GAPGHHGNNLKTKLCENFAKGSCTFGDRCHFAHGAVELRK 993
Score = 46.6 bits (109), Expect = 8e-06
Identities = 23/64 (35%), Positives = 37/64 (56%), Gaps = 7/64 (10%)
Frame = +1
Query: 171 ILNVSSVSGPPGKNITSQTSAPANN------FKTKLCENF-TKGSCTFGERCHFAHGTDE 223
++N++ + P +N+ + +A A N K+++C F T C FG++CHFAHG E
Sbjct: 301 MMNLTPAAPPAPRNVPAPRNAHAPNGSAPSAVKSRICSKFNTAEGCKFGDKCHFAHGEWE 480
Query: 224 LRKP 227
L KP
Sbjct: 481 LGKP 492
Score = 36.2 bits (82), Expect = 0.011
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +1
Query: 32 AKTRLCNKFNTAEGCKFGDKCHFAH 56
+K++ C KF + GC FG+ CHF H
Sbjct: 193 SKSKPCTKFFSTSGCPFGESCHFLH 267
Score = 29.3 bits (64), Expect = 1.3
Identities = 13/24 (54%), Positives = 14/24 (58%), Gaps = 1/24 (4%)
Frame = +1
Query: 197 KTKLCENFTKGS-CTFGERCHFAH 219
K+K C F S C FGE CHF H
Sbjct: 196 KSKPCTKFFSTSGCPFGESCHFLH 267
>TC91951 similar to GP|5441893|dbj|BAA82391.1 ESTs C99174(E10437)
D22295(C10709) correspond to a region of the predicted
gene.~Similar to, partial (23%)
Length = 526
Score = 131 bits (329), Expect = 2e-31
Identities = 65/100 (65%), Positives = 74/100 (74%), Gaps = 3/100 (3%)
Frame = +3
Query: 133 TGAKLSIREHDSDPNLRNIELEGSFDQIKQASAMVHDLILNVSSVSGP---PGKNITSQT 189
TG K SIR+H++DPNLRNIELEG+FDQI QAS MV DL+L +S + P PG
Sbjct: 3 TGVKFSIRDHETDPNLRNIELEGTFDQIAQASNMVKDLLLTLSVSAPPKSTPGAPAGGGP 182
Query: 190 SAPANNFKTKLCENFTKGSCTFGERCHFAHGTDELRKPGM 229
+AP N KTKLCENF KGSCTFGERCHFAHG ELRK G+
Sbjct: 183 AAPGRNIKTKLCENFAKGSCTFGERCHFAHGAAELRKLGV 302
Score = 38.9 bits (89), Expect = 0.002
Identities = 22/50 (44%), Positives = 26/50 (52%), Gaps = 5/50 (10%)
Frame = +3
Query: 17 PNVPQSFPDGSSPPVA----KTRLCNKFNTAEG-CKFGDKCHFAHGEWEL 61
P P G P KT+LC F A+G C FG++CHFAHG EL
Sbjct: 144 PKSTPGAPAGGGPAAPGRNIKTKLCENF--AKGSCTFGERCHFAHGAAEL 287
>BE998796 similar to GP|5441893|dbj| ESTs C99174(E10437) D22295(C10709)
correspond to a region of the predicted gene.~Similar
to, partial (28%)
Length = 609
Score = 131 bits (329), Expect = 2e-31
Identities = 66/122 (54%), Positives = 84/122 (68%), Gaps = 8/122 (6%)
Frame = +1
Query: 7 SPAIPPIGRNPNVPQSF--PDGSSPPVAKTRLCNKFNTAEGCKFGDKCHFAHGEWELGRP 64
+PA PP RN P++ P+GS+P K+R+C+KFNTAEGCKFGDKCHFAHGEWELG+P
Sbjct: 256 TPAAPPAPRNVPAPRNAHAPNGSAPSAVKSRICSKFNTAEGCKFGDKCHFAHGEWELGKP 435
Query: 65 TVPAYE-----DTRAMGQMQSSSVGG-RIEPPPPAHGAAAGFGVSATATVSINATLAGAI 118
P+++ D R MG + GG R+EPPP A FG +ATA +S+ A+LAGAI
Sbjct: 436 VAPSFDDHRHNDHRHMGPPNAGRFGGHRMEPPP----VPASFGANATAKISVEASLAGAI 603
Query: 119 IG 120
IG
Sbjct: 604 IG 609
Score = 46.6 bits (109), Expect = 8e-06
Identities = 23/64 (35%), Positives = 37/64 (56%), Gaps = 7/64 (10%)
Frame = +1
Query: 171 ILNVSSVSGPPGKNITSQTSAPANN------FKTKLCENF-TKGSCTFGERCHFAHGTDE 223
++N++ + P +N+ + +A A N K+++C F T C FG++CHFAHG E
Sbjct: 244 MMNLTPAAPPAPRNVPAPRNAHAPNGSAPSAVKSRICSKFNTAEGCKFGDKCHFAHGEWE 423
Query: 224 LRKP 227
L KP
Sbjct: 424 LGKP 435
Score = 26.9 bits (58), Expect = 6.5
Identities = 8/12 (66%), Positives = 9/12 (74%)
Frame = +1
Query: 45 GCKFGDKCHFAH 56
GC FG+ CHF H
Sbjct: 175 GCPFGESCHFLH 210
>TC91486 similar to GP|5441893|dbj|BAA82391.1 ESTs C99174(E10437)
D22295(C10709) correspond to a region of the predicted
gene.~Similar to, partial (25%)
Length = 736
Score = 114 bits (284), Expect = 4e-26
Identities = 57/114 (50%), Positives = 73/114 (64%), Gaps = 1/114 (0%)
Frame = +2
Query: 8 PAIPPIGRNPNVPQSFPD-GSSPPVAKTRLCNKFNTAEGCKFGDKCHFAHGEWELGRPTV 66
PA PP RN P + GS+ K+R+CNKFN+ EGCKFGDKCHFAHGEWELG+P
Sbjct: 407 PAAPP--RNVAAPPPVSNNGSAQHAVKSRICNKFNSLEGCKFGDKCHFAHGEWELGKPYA 580
Query: 67 PAYEDTRAMGQMQSSSVGGRIEPPPPAHGAAAGFGVSATATVSINATLAGAIIG 120
P+ +D R M + GR+E P G + FG ++TA + + A+LAGAIIG
Sbjct: 581 PSVDDHRLMAPTNVGRMPGRVEAPSGLGGTS--FGANSTAKIXVKASLAGAIIG 736
Score = 41.2 bits (95), Expect = 3e-04
Identities = 20/53 (37%), Positives = 29/53 (53%), Gaps = 1/53 (1%)
Frame = +2
Query: 176 SVSGPPGKNITSQTSAPANNFKTKLCENFTK-GSCTFGERCHFAHGTDELRKP 227
+V+ PP S + + K+++C F C FG++CHFAHG EL KP
Sbjct: 425 NVAAPPP---VSNNGSAQHAVKSRICNKFNSLEGCKFGDKCHFAHGEWELGKP 574
Score = 38.1 bits (87), Expect = 0.003
Identities = 20/62 (32%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = +2
Query: 32 AKTRLCNKFNTAEGCKFGDKCHFAHGEWELGRPTVP-AYEDTRAMGQMQSSSVGGRIEPP 90
+KT+ C KF + GC FG+ CHF+H VP Y M ++ ++ + P
Sbjct: 284 SKTKPCTKFFSIAGCPFGEGCHFSH--------HVPGGYNAVAQMMNLKPAAPPRNVAAP 439
Query: 91 PP 92
PP
Sbjct: 440 PP 445
Score = 30.4 bits (67), Expect = 0.59
Identities = 13/24 (54%), Positives = 14/24 (58%), Gaps = 1/24 (4%)
Frame = +2
Query: 197 KTKLCENFTK-GSCTFGERCHFAH 219
KTK C F C FGE CHF+H
Sbjct: 287 KTKPCTKFFSIAGCPFGEGCHFSH 358
>TC84988 similar to GP|14018368|emb|CAC38358. zinc finger protein {Pisum
sativum}, partial (28%)
Length = 656
Score = 47.8 bits (112), Expect = 4e-06
Identities = 21/55 (38%), Positives = 33/55 (59%), Gaps = 2/55 (3%)
Frame = +1
Query: 175 SSVSGPPGKNITSQTSAPANN--FKTKLCENFTKGSCTFGERCHFAHGTDELRKP 227
S + PP + I + ++ FKT++C F G+C GE C++AHG DE+R+P
Sbjct: 421 SGLMCPPSRMIQPPPNRGTSSIFFKTRICTKFRFGTCRNGENCNYAHGADEIRQP 585
Score = 35.8 bits (81), Expect = 0.014
Identities = 16/39 (41%), Positives = 24/39 (61%)
Frame = +1
Query: 26 GSSPPVAKTRLCNKFNTAEGCKFGDKCHFAHGEWELGRP 64
G+S KTR+C KF C+ G+ C++AHG E+ +P
Sbjct: 472 GTSSIFFKTRICTKFRFGT-CRNGENCNYAHGADEIRQP 585
>BI265088
Length = 635
Score = 35.8 bits (81), Expect = 0.014
Identities = 16/31 (51%), Positives = 19/31 (60%)
Frame = +3
Query: 196 FKTKLCENFTKGSCTFGERCHFAHGTDELRK 226
FKTKLC + G C + C FAHG ELR+
Sbjct: 135 FKTKLCALYQNGRCN-RQNCSFAHGNTELRR 224
>BF635696
Length = 545
Score = 35.8 bits (81), Expect = 0.014
Identities = 16/31 (51%), Positives = 19/31 (60%)
Frame = +2
Query: 196 FKTKLCENFTKGSCTFGERCHFAHGTDELRK 226
FKTKLC + G C + C FAHG ELR+
Sbjct: 134 FKTKLCALYQNGRCN-RQNCSFAHGNTELRR 223
>TC87072 similar to PIR|D84581|D84581 probable CCCH-type zinc finger protein
[imported] - Arabidopsis thaliana, partial (47%)
Length = 1599
Score = 35.4 bits (80), Expect = 0.018
Identities = 14/29 (48%), Positives = 17/29 (58%)
Frame = +3
Query: 195 NFKTKLCENFTKGSCTFGERCHFAHGTDE 223
N+ C +F KGSC G+ C FAHG E
Sbjct: 387 NYSGTSCPDFRKGSCKKGDSCEFAHGVFE 473
Score = 27.7 bits (60), Expect = 3.8
Identities = 10/15 (66%), Positives = 11/15 (72%)
Frame = +3
Query: 46 CKFGDKCHFAHGEWE 60
CK GD C FAHG +E
Sbjct: 429 CKKGDSCEFAHGVFE 473
>TC92272
Length = 811
Score = 34.7 bits (78), Expect = 0.031
Identities = 21/75 (28%), Positives = 38/75 (50%), Gaps = 1/75 (1%)
Frame = +1
Query: 103 SATATVSINATLAGAIIGKNDVNSKQICHITGAKLSIRE-HDSDPNLRNIELEGSFDQIK 161
+ T + + + G++ G+N N Q+ I+GAK+ E + I L G+ D+ +
Sbjct: 259 NTTVEIMLPNDIIGSVYGENGSNLDQLRQISGAKVVFHEPRPGTSDRAKIVLSGTPDETQ 438
Query: 162 QASAMVHDLILNVSS 176
A +++ ILN SS
Sbjct: 439 AAQSLLQAYILNGSS 483
>TC77687 similar to PIR|D84581|D84581 probable CCCH-type zinc finger protein
[imported] - Arabidopsis thaliana, partial (35%)
Length = 1096
Score = 34.7 bits (78), Expect = 0.031
Identities = 15/30 (50%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = +3
Query: 195 NFKTKLCENFTK-GSCTFGERCHFAHGTDE 223
N+ C F K G+CT G+ CHFAHG E
Sbjct: 315 NYSGNPCPEFRKLGNCTKGDSCHFAHGVFE 404
Score = 32.3 bits (72), Expect = 0.15
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +3
Query: 37 CNKFNTAEGCKFGDKCHFAHGEWE 60
C +F C GD CHFAHG +E
Sbjct: 333 CPEFRKLGNCTKGDSCHFAHGVFE 404
>BG646281 weakly similar to PIR|E84465|E844 hypothetical protein At2g05160
[imported] - Arabidopsis thaliana, partial (15%)
Length = 637
Score = 33.1 bits (74), Expect = 0.091
Identities = 13/39 (33%), Positives = 20/39 (50%)
Frame = +2
Query: 182 GKNITSQTSAPANNFKTKLCENFTKGSCTFGERCHFAHG 220
GK +++ + F K+C F+KG C G C + HG
Sbjct: 338 GKATKRFSNSSMSEFPLKICHYFSKGYCRHGGNCRYFHG 454
>TC92368 weakly similar to GP|19347974|gb|AAL86319.1 unknown protein
{Arabidopsis thaliana}, partial (63%)
Length = 999
Score = 32.3 bits (72), Expect = 0.15
Identities = 14/25 (56%), Positives = 17/25 (68%), Gaps = 1/25 (4%)
Frame = +3
Query: 199 KLCENF-TKGSCTFGERCHFAHGTD 222
KLC F + GSC +GE C+F H TD
Sbjct: 87 KLCFKFVSSGSCQWGETCNFRHDTD 161
Score = 28.5 bits (62), Expect = 2.2
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +3
Query: 32 AKTRLCNKFNTAEGCKFGDKCHFAH 56
A +LC KF ++ C++G+ C+F H
Sbjct: 78 AGDKLCFKFVSSGSCQWGETCNFRH 152
>TC76784 similar to PIR|G84825|G84825 probable CCCH-type zinc finger protein
[imported] - Arabidopsis thaliana, partial (56%)
Length = 2631
Score = 32.3 bits (72), Expect = 0.15
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = +3
Query: 201 CENFTKGSCTFGERCHFAHGTDE 223
C F KGSC G+ C +AHG E
Sbjct: 1002 CPEFRKGSCQKGDSCEYAHGVFE 1070
>BI271089 similar to GP|9294325|dbj| gene_id:K24M9.13~unknown protein
{Arabidopsis thaliana}, partial (2%)
Length = 481
Score = 31.6 bits (70), Expect = 0.26
Identities = 11/27 (40%), Positives = 15/27 (54%)
Frame = +3
Query: 201 CENFTKGSCTFGERCHFAHGTDELRKP 227
C+ F G+C G+ C F+H T R P
Sbjct: 171 CKFFASGNCRNGKHCRFSHDTQACRSP 251
>TC91115 similar to PIR|D96705|D96705 probable zinc finger protein T22E19.17
[imported] - Arabidopsis thaliana, partial (11%)
Length = 642
Score = 31.2 bits (69), Expect = 0.34
Identities = 13/32 (40%), Positives = 17/32 (52%), Gaps = 1/32 (3%)
Frame = +2
Query: 196 FKTKLCENFTKGS-CTFGERCHFAHGTDELRK 226
+KT++C G C +G RCHF H E K
Sbjct: 14 YKTEVCRMVLAGVVCPYGHRCHFRHALTEQEK 109
Score = 27.7 bits (60), Expect = 3.8
Identities = 10/27 (37%), Positives = 13/27 (48%)
Frame = +2
Query: 30 PVAKTRLCNKFNTAEGCKFGDKCHFAH 56
P KT +C C +G +CHF H
Sbjct: 8 PRYKTEVCRMVLAGVVCPYGHRCHFRH 88
>TC80592 similar to GP|19310435|gb|AAL84954.1 F17H15.1/F17H15.1 {Arabidopsis
thaliana}, partial (30%)
Length = 945
Score = 31.2 bits (69), Expect = 0.34
Identities = 19/72 (26%), Positives = 34/72 (46%), Gaps = 4/72 (5%)
Frame = +2
Query: 113 TLAGAIIGKNDVNSKQICHITGAKLSIREHDSDP----NLRNIELEGSFDQIKQASAMVH 168
T G IIGK K + TGA++ + P R +++EG+ +QI+ A +V
Sbjct: 668 TRVGLIIGKGGETIKSMQATTGARIQVIPLHLPPGDTSTERTLKIEGTSEQIESAKQLVD 847
Query: 169 DLILNVSSVSGP 180
++ + + P
Sbjct: 848 SILSGENRLRNP 883
>TC77987 weakly similar to GP|17979277|gb|AAL49864.1 unknown protein
{Arabidopsis thaliana}, partial (53%)
Length = 1338
Score = 30.8 bits (68), Expect = 0.45
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +3
Query: 200 LCENFTKGSCTFGERCHFAH 219
+C + KGSC +G RC + H
Sbjct: 177 ICSYYQKGSCAYGSRCRYKH 236
>AJ501021 similar to PIR|C84587|C84 hypothetical protein At2g20280 [imported]
- Arabidopsis thaliana, partial (42%)
Length = 611
Score = 30.8 bits (68), Expect = 0.45
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 192 PANNFKTKLCENFTKGSCTFGERCHFAHGTDELRK 226
P + K+ LCE F G C G +C F+H + RK
Sbjct: 371 PGVDPKSVLCEFFKVGQCAKGFKCKFSHDLNVQRK 475
>TC82778 similar to PIR|T04011|T04011 hypothetical protein T5L19.200 -
Arabidopsis thaliana, partial (7%)
Length = 784
Score = 30.4 bits (67), Expect = 0.59
Identities = 17/59 (28%), Positives = 34/59 (56%), Gaps = 3/59 (5%)
Frame = +3
Query: 103 SATATVSINATLAGAIIGKNDVNSKQICHITGAKLSI-REHDSDP--NLRNIELEGSFD 158
+ T + + + G +IGK+ + + + +GAK+ I R+ D+DP + R +EL G+ +
Sbjct: 603 TTTRKIEVPSNKVGVLIGKSGDTIRYLQYNSGAKIQITRDADADPHSSTRPVELIGTLE 779
>TC86255 similar to PIR|D84581|D84581 probable CCCH-type zinc finger protein
[imported] - Arabidopsis thaliana, partial (47%)
Length = 1508
Score = 30.0 bits (66), Expect = 0.77
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +3
Query: 201 CENFTKGSCTFGERCHFAHGTDE 223
C +F KG+C G+ C AHG E
Sbjct: 483 CPDFRKGNCKKGDACEHAHGVFE 551
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.314 0.132 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,895,393
Number of Sequences: 36976
Number of extensions: 118587
Number of successful extensions: 654
Number of sequences better than 10.0: 61
Number of HSP's better than 10.0 without gapping: 568
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 642
length of query: 229
length of database: 9,014,727
effective HSP length: 93
effective length of query: 136
effective length of database: 5,575,959
effective search space: 758330424
effective search space used: 758330424
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 56 (26.2 bits)
Medicago: description of AC149471.8