BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149471.20 + phase: 0
(227 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC81732 weakly similar to PIR|B84862|B84862 hypothetical protein... 272 e-105
TC76342 similar to PIR|A86444|A86444 probable RNA helicase [impo... 34 0.052
TC88436 similar to GP|9293867|dbj|BAB01770.1 DEAD-Box RNA helica... 33 0.089
TC76962 similar to GP|10241935|dbj|BAB13711. elicitor inducible ... 27 0.10
TC79977 similar to PIR|T52337|T52337 phosphoprotein phosphatase ... 32 0.26
TC78036 homologue to PIR|T06824|T06824 translation initiation fa... 30 0.58
TC87237 similar to GP|160409|gb|AAA29651.1|| mature-parasite-inf... 30 0.99
TC82857 similar to PIR|T52076|T52076 ABI3-interacting protein ai... 30 0.99
AW696399 homologue to PIR|T06370|T0 probable DNA (cytosine-5-)-m... 29 1.3
TC77435 similar to GP|7211427|gb|AAF40306.1| RNA helicase {Vigna... 29 1.3
TC89584 weakly similar to GP|18076679|emb|CAC84774. P70 protein ... 29 1.7
TC89267 similar to GP|20856975|gb|AAM26693.1 At5g26743 {Arabidop... 28 2.2
TC91820 homologue to GP|23617110|dbj|BAC20792. contains ESTs C27... 28 2.2
TC79326 homologue to GP|14190381|gb|AAK55671.1 AT5g11200/F2I11_9... 28 2.9
CB065330 similar to PIR|E83588|E83 RNA helicase DbpA PA0455 [imp... 28 2.9
TC77964 homologue to GP|14190381|gb|AAK55671.1 AT5g11200/F2I11_9... 28 2.9
AL383186 28 3.8
TC88075 homologue to GP|2346978|dbj|BAA21923.1 ZPT2-14 {Petunia ... 28 3.8
TC83922 similar to GP|15809990|gb|AAL06922.1 AT5g60530/muf9_180 ... 28 3.8
BE997545 weakly similar to GP|13872902|db putative protein kinas... 27 8.4
>TC81732 weakly similar to PIR|B84862|B84862 hypothetical protein At2g43110
[imported] - Arabidopsis thaliana, partial (46%)
Length = 637
Score = 272 bits (695), Expect(2) = e-105
Identities = 137/139 (98%), Positives = 138/139 (98%)
Frame = +2
Query: 1 MGKRKGRENNDDRRKKKKKKTETTGSEQLRFFVNEFQSANDVQLSSIELESLKADSSILE 60
MGKRKGRENNDDRRKKKKKKTETTGSEQLRFFVNEFQSANDVQLSSIELESLKADSSILE
Sbjct: 17 MGKRKGRENNDDRRKKKKKKTETTGSEQLRFFVNEFQSANDVQLSSIELESLKADSSILE 196
Query: 61 LSNSQNSRDTDLDVKLLGGDIKGAFGNCWRQVLCESEVVEGKIPPGSPSVLIVSPSALRS 120
LSNSQNSRDTDLDVKLLGGDIKGAFGNCWRQVLCESEVVEGKIPPGSPSVLIVSPSALRS
Sbjct: 197 LSNSQNSRDTDLDVKLLGGDIKGAFGNCWRQVLCESEVVEGKIPPGSPSVLIVSPSALRS 376
Query: 121 IHLLKGFRFMTKQCSAVKL 139
IHLLKGFRFMTKQCSAV +
Sbjct: 377 IHLLKGFRFMTKQCSAVNV 433
Score = 127 bits (320), Expect(2) = e-105
Identities = 65/66 (98%), Positives = 66/66 (99%)
Frame = +3
Query: 139 LFSKHIKLQEQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLDMHPDVKGYSLF 198
LFSKHIKLQEQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLDMHPDVKGYSLF
Sbjct: 432 LFSKHIKLQEQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLDMHPDVKGYSLF 611
Query: 199 TLPQVR 204
TLPQV+
Sbjct: 612 TLPQVK 629
>TC76342 similar to PIR|A86444|A86444 probable RNA helicase [imported] -
Arabidopsis thaliana, partial (80%)
Length = 1651
Score = 33.9 bits (76), Expect = 0.052
Identities = 16/39 (41%), Positives = 25/39 (64%)
Frame = +3
Query: 149 QISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
QIS LK+ ++I GTP RI+ L+++ L + +VLD
Sbjct: 717 QISALKSGIDIVIGTPGRIQDLVEMGICRLQEVSFVVLD 833
>TC88436 similar to GP|9293867|dbj|BAB01770.1 DEAD-Box RNA helicase-like
protein {Arabidopsis thaliana}, partial (38%)
Length = 1024
Score = 33.1 bits (74), Expect = 0.089
Identities = 22/80 (27%), Positives = 40/80 (49%)
Frame = +1
Query: 108 PSVLIVSPSALRSIHLLKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPSRI 167
P L+++P+ + + K F + ++ + +Q+ L V+IA GTP RI
Sbjct: 529 PLALVLAPTRELAKQVEKEFYEAAPNLDTICVYGG-TPISQQMRQLDYGVDIAVGTPGRI 705
Query: 168 KKLIDVEALGLSRLQVLVLD 187
L++ AL L +Q ++LD
Sbjct: 706 IDLLNRGALNLKEVQFVILD 765
>TC76962 similar to GP|10241935|dbj|BAB13711. elicitor inducible protein
{Nicotiana tabacum}, partial (76%)
Length = 1024
Score = 27.3 bits (59), Expect(2) = 0.10
Identities = 16/48 (33%), Positives = 29/48 (60%), Gaps = 4/48 (8%)
Frame = +2
Query: 42 VQLSSIELESLKADSS----ILELSNSQNSRDTDLDVKLLGGDIKGAF 85
+ L++ ++ + DSS +L L +S NSRD+D V++L D++ F
Sbjct: 77 LDLANSQVNTEDTDSSNQKLVLTLYDSLNSRDSDTVVRILAPDLEWWF 220
Score = 24.3 bits (51), Expect(2) = 0.10
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = +3
Query: 104 PPGSPSVLIVSPSALRSIHLL 124
PP PSVL +SPS+L + LL
Sbjct: 273 PPLIPSVLFLSPSSLSAPPLL 335
>TC79977 similar to PIR|T52337|T52337 phosphoprotein phosphatase (EC
3.1.3.16) 2C [imported] - common ice plant, partial
(85%)
Length = 1422
Score = 31.6 bits (70), Expect = 0.26
Identities = 40/152 (26%), Positives = 69/152 (45%), Gaps = 6/152 (3%)
Frame = +3
Query: 74 VKLLGGDIKGAFGNCWRQVLCESEVVEGKIPPGSPSVLIVSPSALRSIHLLKGFRFMTKQ 133
V+ LGG ++ + N V + K+P G+PS LI P R + L + F+
Sbjct: 825 VEELGGYVEDGYLNGVLSVTRALGDWDMKLPRGTPSPLIADPE-FRQVVLTEDDEFLIIG 1001
Query: 134 CSAVKLFSKHIKLQEQISLLKNRVNIASGTPSRIKKLIDVEALGLS---RLQVLVLDMHP 190
C + + Q +SL++ + P + + + +EAL L+ L V+++ +
Sbjct: 1002CDGI---WDVMTSQHAVSLVRKGLR-RHDDPEKCARDLVMEALRLNTFDNLTVIIICLSS 1169
Query: 191 DVKGYSLFTLP---QVRCASLSSPYLFCLDSL 219
G S + P ++RC SLS+ L L SL
Sbjct: 1170LDHGESEPSPPPQRKLRCCSLSAEALCSLRSL 1265
>TC78036 homologue to PIR|T06824|T06824 translation initiation factor -
garden pea, complete
Length = 1571
Score = 30.4 bits (67), Expect = 0.58
Identities = 15/40 (37%), Positives = 24/40 (59%)
Frame = +3
Query: 148 EQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
E I L++ V++ SGTP R+ +I L +++LVLD
Sbjct: 513 EDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKLLVLD 632
>TC87237 similar to GP|160409|gb|AAA29651.1|| mature-parasite-infected
erythrocyte surface antigen {Plasmodium falciparum},
partial (2%)
Length = 2007
Score = 29.6 bits (65), Expect = 0.99
Identities = 28/100 (28%), Positives = 39/100 (39%), Gaps = 3/100 (3%)
Frame = +1
Query: 8 ENNDDRRKKKKKKTETT---GSEQLRFFVNEFQSANDVQLSSIELESLKADSSILELSNS 64
ENN D + K E T G+ VNE Q+ N Q + E E + S+ +
Sbjct: 1426 ENNSDENQNDSKNNENTNDSGNTSNDANVNENQNENAAQTKTSENEGDAQNESVESKKEN 1605
Query: 65 QNSRDTDLDVKLLGGDIKGAFGNCWRQVLCESEVVEGKIP 104
S D+D D +G+ Q ES V G +P
Sbjct: 1606 NESAHKDVDNNSNSND-QGSSDTSVTQDDKESRVDLGTLP 1722
>TC82857 similar to PIR|T52076|T52076 ABI3-interacting protein aip1
[imported] - Arabidopsis thaliana, partial (11%)
Length = 818
Score = 29.6 bits (65), Expect = 0.99
Identities = 17/45 (37%), Positives = 29/45 (63%)
Frame = -1
Query: 17 KKKKTETTGSEQLRFFVNEFQSANDVQLSSIELESLKADSSILEL 61
K+KKT ++G QLRF +++S +SSIEL++ S+ L++
Sbjct: 767 KEKKTHSSG--QLRFLNKKYESTASAFISSIELKNGMKLSTALDI 639
>AW696399 homologue to PIR|T06370|T0 probable DNA
(cytosine-5-)-methyltransferase (EC 2.1.1.37) - garden
pea, partial (11%)
Length = 553
Score = 29.3 bits (64), Expect = 1.3
Identities = 21/97 (21%), Positives = 42/97 (42%), Gaps = 2/97 (2%)
Frame = +2
Query: 14 RKKKKKKTETTGSEQLRFFV--NEFQSANDVQLSSIELESLKADSSILELSNSQNSRDTD 71
+ KK++K + S +F++ NE + AND L + SL+ + N + DT+
Sbjct: 125 KSKKQRKNPRSSSSSNKFYIKINEDEIANDYPLPAYYKTSLQETDEFIVFDNDYDVYDTE 304
Query: 72 LDVKLLGGDIKGAFGNCWRQVLCESEVVEGKIPPGSP 108
D+ + + W +S ++ ++ P P
Sbjct: 305 --------DLPRSMLHNWALYNSDSRLISLELLPMKP 391
>TC77435 similar to GP|7211427|gb|AAF40306.1| RNA helicase {Vigna radiata},
partial (67%)
Length = 1828
Score = 29.3 bits (64), Expect = 1.3
Identities = 17/39 (43%), Positives = 23/39 (58%)
Frame = +1
Query: 149 QISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
Q + L+ V+I GTP RIK I+ + LS L+ VLD
Sbjct: 781 QETKLRRGVDIVIGTPGRIKDHIERGNIDLSHLKFRVLD 897
>TC89584 weakly similar to GP|18076679|emb|CAC84774. P70 protein {Nicotiana
tabacum}, partial (26%)
Length = 872
Score = 28.9 bits (63), Expect = 1.7
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = +3
Query: 17 KKKKTETTGSEQLRFFVNEFQSANDVQLSSIELESLKADSSILELSNSQNSRDTDL 72
KK K + SE LR +L S+ E ++ +LELSNS++ R +L
Sbjct: 507 KKAKEQLNSSESLRIKAQHEAEDAKNRLLSMSRELEESQRQLLELSNSEDERIQEL 674
>TC89267 similar to GP|20856975|gb|AAM26693.1 At5g26743 {Arabidopsis
thaliana}, partial (20%)
Length = 807
Score = 28.5 bits (62), Expect = 2.2
Identities = 15/39 (38%), Positives = 22/39 (55%)
Frame = +3
Query: 149 QISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
Q + L V++ GTP R+ LI+ L + +Q LVLD
Sbjct: 636 QRNALSRGVDVVVGTPGRLIDLINGGTLKFAEIQYLVLD 752
>TC91820 homologue to GP|23617110|dbj|BAC20792. contains ESTs C27700(C52636)
AU222511(C52636)~transcription factor (PWWP domain
protein) -like, partial (3%)
Length = 870
Score = 28.5 bits (62), Expect = 2.2
Identities = 18/58 (31%), Positives = 32/58 (55%), Gaps = 2/58 (3%)
Frame = -1
Query: 89 WRQ-VLCESEVVEG-KIPPGSPSVLIVSPSALRSIHLLKGFRFMTKQCSAVKLFSKHI 144
WR+ ++C E++ ++ PG +L+ SPS + +L RF +CS+ SKH+
Sbjct: 294 WRKAIICGGELISSLQLFPGVCGMLLSSPSVEDDLPILTLHRFTRGKCSSPSC-SKHL 124
>TC79326 homologue to GP|14190381|gb|AAK55671.1 AT5g11200/F2I11_90
{Arabidopsis thaliana}, partial (94%)
Length = 1883
Score = 28.1 bits (61), Expect = 2.9
Identities = 26/86 (30%), Positives = 39/86 (45%), Gaps = 3/86 (3%)
Frame = +2
Query: 105 PGSPSVLIVSPSALRSIHLLKGF-RFMTKQCSA-VKLFSKHIKLQEQISLLKNRV-NIAS 161
PG S LI+ + + + F RF T V +F + ++ LLKN +I
Sbjct: 434 PGQVSALILCHTRELAYQICHEFERFSTYLADLKVAVFYGGVNIKVHKDLLKNECPHIVV 613
Query: 162 GTPSRIKKLIDVEALGLSRLQVLVLD 187
GTP RI L + L L ++ +LD
Sbjct: 614 GTPGRILALARDKDLSLKNVRHFILD 691
>CB065330 similar to PIR|E83588|E83 RNA helicase DbpA PA0455 [imported] -
Pseudomonas aeruginosa (strain PAO1), partial (24%)
Length = 335
Score = 28.1 bits (61), Expect = 2.9
Identities = 15/35 (42%), Positives = 21/35 (59%)
Frame = +1
Query: 153 LKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
L++ +I GTP RI++ +D L L L LVLD
Sbjct: 7 LEHGAHIIVGTPGRIQQHLDKGTLVLDGLNTLVLD 111
>TC77964 homologue to GP|14190381|gb|AAK55671.1 AT5g11200/F2I11_90
{Arabidopsis thaliana}, partial (97%)
Length = 1454
Score = 28.1 bits (61), Expect = 2.9
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = +1
Query: 137 VKLFSKHIKLQEQISLLKNRV-NIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
V +F + ++ LLKN +I GTP RI L + LGL ++ +LD
Sbjct: 517 VAVFYGGVNIKVHKDLLKNECPHIVVGTPGRILALTRDKDLGLKNVRHFILD 672
>AL383186
Length = 505
Score = 27.7 bits (60), Expect = 3.8
Identities = 10/18 (55%), Positives = 15/18 (82%)
Frame = -2
Query: 3 KRKGRENNDDRRKKKKKK 20
KR+ + NN+D+R KK+KK
Sbjct: 87 KRESKVNNEDKRNKKRKK 34
>TC88075 homologue to GP|2346978|dbj|BAA21923.1 ZPT2-14 {Petunia x hybrida},
partial (38%)
Length = 674
Score = 27.7 bits (60), Expect = 3.8
Identities = 17/45 (37%), Positives = 25/45 (54%), Gaps = 2/45 (4%)
Frame = +3
Query: 45 SSIELESLKADSSILELSNSQNSRDTDLDVKL--LGGDIKGAFGN 87
SSI + + S +L+ SNS+ +LD+ L L D+K FGN
Sbjct: 378 SSINYQVIAKSSPVLKRSNSKRVMIMELDLNLTPLENDLKLLFGN 512
>TC83922 similar to GP|15809990|gb|AAL06922.1 AT5g60530/muf9_180
{Arabidopsis thaliana}, partial (21%)
Length = 619
Score = 27.7 bits (60), Expect = 3.8
Identities = 12/29 (41%), Positives = 18/29 (61%)
Frame = +1
Query: 2 GKRKGRENNDDRRKKKKKKTETTGSEQLR 30
GK KG+E +KKK K E++ +QL+
Sbjct: 205 GKEKGKEKEKAPKKKKAPKEESSNYQQLQ 291
>BE997545 weakly similar to GP|13872902|db putative protein kinase Xa21
{Oryza sativa (japonica cultivar-group)}, partial (11%)
Length = 408
Score = 26.6 bits (57), Expect = 8.4
Identities = 23/79 (29%), Positives = 32/79 (40%), Gaps = 15/79 (18%)
Frame = +2
Query: 47 IELESLKADSSILELSNSQNS-------------RDTDLDVKLLGGDIKGAFGNCWR--Q 91
+E+ SL + S++L LS + S D+ LL GDI A G C R
Sbjct: 41 LEVFSLSSLSNLLNLSRNSLSGSLPREVGMLKSINKLDVSENLLSGDIPRAIGECIRLEY 220
Query: 92 VLCESEVVEGKIPPGSPSV 110
+ + G IP SV
Sbjct: 221 LFLQGNSFNGTIPSSLASV 277
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.134 0.373
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,334,070
Number of Sequences: 36976
Number of extensions: 80106
Number of successful extensions: 802
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 743
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 778
length of query: 227
length of database: 9,014,727
effective HSP length: 93
effective length of query: 134
effective length of database: 5,575,959
effective search space: 747178506
effective search space used: 747178506
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC149471.20