BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149305.9 - phase: 0 /pseudo
(136 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ148771 51 1e-07
TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarot... 40 2e-04
TC82948 37 0.002
BG584442 36 0.004
TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non... 32 0.10
AJ501946 similar to PIR|B84807|B848 probable RNA-binding protein... 27 1.9
BG586778 weakly similar to PIR|C86408|C86 hypothetical protein A... 27 3.3
TC90193 homologue to PIR|F85355|F85355 hypothetical protein AT4g... 27 3.3
BG586489 26 4.3
AJ497561 weakly similar to GP|10140689|gb putative non-LTR retro... 26 4.3
TC77342 similar to GP|13785211|emb|CAC37357. putative membrane p... 26 5.6
TC88261 similar to PIR|S22697|S22697 extensin - Volvox carteri (... 25 7.3
TC76818 homologue to GP|13265501|gb|AAG40043.2 AT3g54890 {Arabid... 25 7.3
TC86285 homologue to SP|P46290|RL31_NICGU 60S ribosomal protein ... 25 7.3
TC76574 homologue to GP|11138320|dbj|BAB17821. vf14-3-3c protein... 25 7.3
BQ141738 similar to GP|13430510|gb| putative translation elongat... 25 9.5
TC81160 similar to GP|14422289|emb|CAC41617. cyclin-dependent ki... 25 9.5
TC79333 25 9.5
TC76948 similar to SP|Q08935|ROC1_NICSY 29 kDa ribonucleoprotein... 25 9.5
BE249146 similar to GP|14422289|emb cyclin-dependent kinase inhi... 25 9.5
>BQ148771
Length = 680
Score = 51.2 bits (121), Expect = 1e-07
Identities = 32/112 (28%), Positives = 58/112 (51%), Gaps = 1/112 (0%)
Frame = -3
Query: 24 LASWKCRLLNKPSRVILVNSVLTSIPAYKMQIH*LAHYICVIDHLNKTVHSFI*K*S-TN 82
LA+WK L+ RV L SV+ ++P Y M + I+ + K F+ + +
Sbjct: 573 LANWKANHLSLARRVTLAKSVIEAVPLYPMMTTIIPK--ACIEEIQKLQRKFVWGDTEVS 400
Query: 83 KGMNMVGWDKITKAKKNGGLGVRVVRKQNIALLGKLVWNLLMPTNHLWSQIL 134
+ + VGW+ ++K K GLG+R + N A + KL W++ +N L ++++
Sbjct: 399 RRYHAVGWETMSKPKTIYGLGLRRLDVMNKACIMKLGWSIYSGSNSLCTEVM 244
>TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarotenoid
dioxygenase1 {Pisum sativum}, partial (43%)
Length = 1865
Score = 40.4 bits (93), Expect = 2e-04
Identities = 19/45 (42%), Positives = 27/45 (59%)
Frame = -3
Query: 90 WDKITKAKKNGGLGVRVVRKQNIALLGKLVWNLLMPTNHLWSQIL 134
W + + K GGLGVR +R N++LL K W LL + LW ++L
Sbjct: 975 WHCLPRCK--GGLGVRDIRLVNVSLLAKWWWRLLQDQSSLWKEVL 847
>TC82948
Length = 705
Score = 37.0 bits (84), Expect = 0.002
Identities = 22/74 (29%), Positives = 38/74 (50%), Gaps = 11/74 (14%)
Frame = +3
Query: 62 ICVIDHLNKTVHSFI*K*STNKGMN-----------MVGWDKITKAKKNGGLGVRVVRKQ 110
+C + ++KT F *K + + +N V W+K+ + K G LG+R + K
Sbjct: 165 VCTVKRIHKT---FT*KSTRREEINH*LALFPGKVVKVSWEKVCRPIKEGSLGIRSLSKL 335
Query: 111 NIALLGKLVWNLLM 124
N AL KL W++++
Sbjct: 336 NEALNLKLCWDMMI 377
>BG584442
Length = 775
Score = 36.2 bits (82), Expect = 0.004
Identities = 29/108 (26%), Positives = 53/108 (48%), Gaps = 2/108 (1%)
Frame = +1
Query: 30 RLLNKPSRVILVNSVLTSIPAYKMQIH*LAHYICVIDHLNKTVHSF--I*K*STNKGMNM 87
+ L+K +++ L SI +Y M I L + +D + K +++F + KGM+
Sbjct: 403 KCLSKVM*EVMIKYALQSISSYVMSIFLLLN--SQVDEIEKIMNTFSWVHVGENRKGMHW 576
Query: 88 VGWDKITKAKKNGGLGVRVVRKQNIALLGKLVWNLLMPTNHLWSQILS 135
+ +K+ K GG+G NI +LGK V + L+ L+ + +S
Sbjct: 577 MS*EKLFVHKNYGGMGFTDFTTFNIPMLGKQV*SFLLNRTTLFLEKIS 720
>TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non-LTR
retroelement reverse transcriptase {Oryza sativa
(japonica cultivar-group)}, partial (2%)
Length = 1262
Score = 31.6 bits (70), Expect = 0.10
Identities = 16/36 (44%), Positives = 21/36 (57%)
Frame = +1
Query: 101 GLGVRVVRKQNIALLGKLVWNLLMPTNHLWSQILSA 136
GLGV N++LLGK W LL+ LW ++L A
Sbjct: 31 GLGVGAF---NLSLLGKWCWRLLVDKEGLWHRVLKA 129
>AJ501946 similar to PIR|B84807|B848 probable RNA-binding protein [imported]
- Arabidopsis thaliana, partial (12%)
Length = 421
Score = 27.3 bits (59), Expect = 1.9
Identities = 11/36 (30%), Positives = 21/36 (57%)
Frame = +3
Query: 1 EKTNHFNIIFKLISLVCGFLINKLASWKCRLLNKPS 36
+KT HF+I F + L+ + N+++S + N P+
Sbjct: 96 QKTLHFSIAFGFVELILSNMYNQISSPSSQRTNSPN 203
>BG586778 weakly similar to PIR|C86408|C86 hypothetical protein AAF98433.1
[imported] - Arabidopsis thaliana, partial (43%)
Length = 730
Score = 26.6 bits (57), Expect = 3.3
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 3/42 (7%)
Frame = +3
Query: 11 KLISLVCGFLINKLASWKCRLLNKPSRVILV---NSVLTSIP 49
+L+ L C L SW R LN PS ++L+ NSV +P
Sbjct: 600 RLLHLHCMDLFCLRLSWFIRRLNNPSLILLLWNFNSVCACLP 725
>TC90193 homologue to PIR|F85355|F85355 hypothetical protein AT4g30410
[imported] - Arabidopsis thaliana, partial (8%)
Length = 751
Score = 26.6 bits (57), Expect = 3.3
Identities = 13/46 (28%), Positives = 26/46 (56%), Gaps = 11/46 (23%)
Frame = -1
Query: 3 TNHFNIIFKLISLVCGF-----LINKLASWK------CRLLNKPSR 37
++HFN+ F+ ++ GF +I++ ++W+ C LN+P R
Sbjct: 616 SHHFNLSFERFYII*GFINQTCIIHEFSTWQQTLEPSCSFLNQPCR 479
>BG586489
Length = 514
Score = 26.2 bits (56), Expect = 4.3
Identities = 14/32 (43%), Positives = 19/32 (58%)
Frame = -1
Query: 44 VLTSIPAYKMQIH*LAHYICVIDHLNKTVHSF 75
VL + +QI L +Y C++ HLN VHSF
Sbjct: 187 VLKVVNQMVVQITLLLNYCCLVSHLN--VHSF 98
>AJ497561 weakly similar to GP|10140689|gb putative non-LTR retroelement
reverse transcriptase {Oryza sativa (japonica
cultivar-group)}, partial (2%)
Length = 621
Score = 26.2 bits (56), Expect = 4.3
Identities = 13/34 (38%), Positives = 23/34 (67%)
Frame = +3
Query: 90 WDKITKAKKNGGLGVRVVRKQNIALLGKLVWNLL 123
++ + + K GGLG++ ++ NI++LGK W LL
Sbjct: 303 FNNLARHKSVGGLGLQN-QEFNISMLGKQRWKLL 401
>TC77342 similar to GP|13785211|emb|CAC37357. putative membrane protein
{Solanum tuberosum}, partial (47%)
Length = 1581
Score = 25.8 bits (55), Expect = 5.6
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = +2
Query: 101 GLGVRVVRKQNIALLGKLVWNLLMPTNHLWSQIL 134
G+G R R+ +L + W +LMPT + ++ L
Sbjct: 707 GIGSRQRRRNTHGVLNAISWGILMPTGAVIARYL 808
>TC88261 similar to PIR|S22697|S22697 extensin - Volvox carteri (fragment),
partial (7%)
Length = 1516
Score = 25.4 bits (54), Expect = 7.3
Identities = 9/30 (30%), Positives = 19/30 (63%)
Frame = +3
Query: 44 VLTSIPAYKMQIH*LAHYICVIDHLNKTVH 73
+L +P + + IH L ++C++ HL+ +H
Sbjct: 303 LLLRVPFHYLLIHLLQPHLCLLHHLHLLLH 392
>TC76818 homologue to GP|13265501|gb|AAG40043.2 AT3g54890 {Arabidopsis
thaliana}, partial (84%)
Length = 1010
Score = 25.4 bits (54), Expect = 7.3
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -3
Query: 7 NIIFKLISLVCGFLINKLASWKCRLLNKPS 36
N++ IS V ++ + +SW C LLN S
Sbjct: 801 NVVVPWISQVSSQILQRSSSWICCLLNNDS 712
>TC86285 homologue to SP|P46290|RL31_NICGU 60S ribosomal protein L31.
[Tobacco] {Nicotiana glutinosa}, partial (95%)
Length = 632
Score = 25.4 bits (54), Expect = 7.3
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +3
Query: 101 GLGVRVVRKQNIALLGKLVWNLLMPTNHLW 130
GLG +V+ ++ LL L+W L + + W
Sbjct: 387 GLGTKVIDDED*CLLALLIWFLYLILSETW 476
>TC76574 homologue to GP|11138320|dbj|BAB17821. vf14-3-3c protein {Vicia
faba}, partial (95%)
Length = 1051
Score = 25.4 bits (54), Expect = 7.3
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +2
Query: 96 AKKNGGLGVRVVRKQNIALLGKLVWNL 122
+ + GGL + VR+ IAL+ KL+ NL
Sbjct: 851 SSEGGGLRMSFVRQLRIALIYKLIGNL 931
>BQ141738 similar to GP|13430510|gb| putative translation elongation factor
eEF-1 alpha chain A4 {Arabidopsis thaliana}, partial
(18%)
Length = 785
Score = 25.0 bits (53), Expect = 9.5
Identities = 17/52 (32%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = +1
Query: 6 FNIIFKLIS-LVCGFLINKLASWKCRLLNKPSRVILVNSVLTSIPAYKMQIH 56
FNI S + C F NK+A+ C LN ++ V + ++SIP + ++H
Sbjct: 475 FNITLGTESKMKCRF--NKMAAINCHSLNA*IKLNPVTNPISSIPIERSRLH 624
>TC81160 similar to GP|14422289|emb|CAC41617. cyclin-dependent kinase
inhibitor 3 {Arabidopsis thaliana}, partial (38%)
Length = 805
Score = 25.0 bits (53), Expect = 9.5
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = +1
Query: 18 GFLINKLASWKCRLLNKPSRVILVNSVL 45
GF+ +L +W+ +L K R+++V S+L
Sbjct: 301 GFVNVRLRNWEISVLKKRMRIVMVISLL 384
>TC79333
Length = 872
Score = 25.0 bits (53), Expect = 9.5
Identities = 8/22 (36%), Positives = 15/22 (67%)
Frame = +1
Query: 6 FNIIFKLISLVCGFLINKLASW 27
+NI + +S++CGF +K+ W
Sbjct: 415 YNICRRSVSILCGFRSSKIVVW 480
>TC76948 similar to SP|Q08935|ROC1_NICSY 29 kDa ribonucleoprotein A
chloroplast precursor (CP29A). [Wood tobacco] {Nicotiana
sylvestris}, partial (61%)
Length = 1374
Score = 25.0 bits (53), Expect = 9.5
Identities = 9/19 (47%), Positives = 16/19 (83%)
Frame = -1
Query: 28 KCRLLNKPSRVILVNSVLT 46
KC+++N PS+V +N+V+T
Sbjct: 726 KCQVVNTPSKVTNMNTVVT 670
>BE249146 similar to GP|14422289|emb cyclin-dependent kinase inhibitor 3
{Arabidopsis thaliana}, partial (12%)
Length = 638
Score = 25.0 bits (53), Expect = 9.5
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = +2
Query: 18 GFLINKLASWKCRLLNKPSRVILVNSVL 45
GF+ +L +W+ +L K R+++V S+L
Sbjct: 308 GFVNVRLRNWEISVLKKRMRIVMVISLL 391
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.337 0.146 0.476
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,175,899
Number of Sequences: 36976
Number of extensions: 67415
Number of successful extensions: 632
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 626
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 631
length of query: 136
length of database: 9,014,727
effective HSP length: 86
effective length of query: 50
effective length of database: 5,834,791
effective search space: 291739550
effective search space used: 291739550
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC149305.9