BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149305.6 - phase: 0 /pseudo/partial
(543 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF634103 weakly similar to GP|20260434|gb putative PHD-type zinc... 42 6e-04
AW560016 similar to GP|8778713|gb T25N20.3 {Arabidopsis thaliana... 41 0.001
TC93640 similar to GP|8978355|dbj|BAA98208.1 gene_id:K3D20.8~unk... 37 0.019
TC76832 weakly similar to GP|21592534|gb|AAM64483.1 unknown {Ara... 34 0.16
BF645370 weakly similar to GP|12321864|gb| RING zinc finger prot... 33 0.28
TC76831 similar to GP|21593924|gb|AAM65889.1 unknown {Arabidopsi... 32 0.48
TC84304 similar to GP|4105798|gb|AAD02556.1| PGPD14 {Petunia x h... 32 0.62
TC93550 weakly similar to GP|20260434|gb|AAM13115.1 putative PHD... 31 1.1
BQ139867 31 1.1
TC87819 similar to GP|16209696|gb|AAL14405.1 AT5g20880/F22D1_50 ... 31 1.1
BQ143956 similar to GP|21592534|gb| unknown {Arabidopsis thalian... 31 1.4
TC86960 similar to PIR|C85253|C85253 receptor like protein (part... 30 2.4
BG645953 similar to PIR|B96625|B96 hypothetical protein T2K10.12... 30 2.4
BE202843 weakly similar to GP|10177512|dbj RING zinc finger prot... 30 3.1
TC77980 similar to PIR|T12970|T12970 hypothetical protein T6H20.... 30 3.1
TC86319 similar to GP|15451110|gb|AAK96826.1 Unknown protein {Ar... 29 4.0
TC77981 similar to PIR|T12970|T12970 hypothetical protein T6H20.... 29 4.0
BF634100 similar to GP|18377636|gb| unknown protein {Arabidopsis... 29 4.0
TC86318 similar to GP|15451110|gb|AAK96826.1 Unknown protein {Ar... 29 4.0
TC81289 weakly similar to PIR|G96791|G96791 probable RING zinc f... 29 4.0
>BF634103 weakly similar to GP|20260434|gb putative PHD-type zinc finger
protein {Arabidopsis thaliana}, partial (3%)
Length = 369
Score = 42.0 bits (97), Expect = 6e-04
Identities = 20/44 (45%), Positives = 23/44 (51%)
Frame = +3
Query: 46 CCVCLGGRVENLFKCSGCDRLFHADCLDVKENEVPNRNWYCLMC 89
C VC G NLF C GC R FH +C V + P R YC +C
Sbjct: 72 CIVCWDGG--NLFLCDGCPRAFHKECASV--SSTPRRGRYCPIC 191
>AW560016 similar to GP|8778713|gb T25N20.3 {Arabidopsis thaliana}, partial
(13%)
Length = 657
Score = 41.2 bits (95), Expect = 0.001
Identities = 24/70 (34%), Positives = 33/70 (46%)
Frame = +1
Query: 44 DTCCVCLGGRVENLFKCSGCDRLFHADCLDVKENEVPNRNWYCLMCICSKQLLVLQSYCN 103
DTC +C G +L C GC FH CLD++ +P W C C C L +
Sbjct: 331 DTCGICGDGG--DLICCDGCPSTFHQSCLDIQ--MLPPGEWRCPNCTCKFCGL---ASAT 489
Query: 104 SQRKDDAKKN 113
+ ++DDA N
Sbjct: 490 TDKEDDATVN 519
>TC93640 similar to GP|8978355|dbj|BAA98208.1 gene_id:K3D20.8~unknown
protein {Arabidopsis thaliana}, partial (1%)
Length = 842
Score = 37.0 bits (84), Expect = 0.019
Identities = 17/46 (36%), Positives = 21/46 (44%), Gaps = 1/46 (2%)
Frame = +1
Query: 45 TCCVCLGGRVENLFKCSGCDRLFHADCLDVKENEVPN-RNWYCLMC 89
+CCVCL G C C L H+ CL V + +W CL C
Sbjct: 661 SCCVCLQGEPGPDLFCETCGTLMHSHCLPTWHEPVSDPEHWRCLTC 798
>TC76832 weakly similar to GP|21592534|gb|AAM64483.1 unknown {Arabidopsis
thaliana}, partial (71%)
Length = 601
Score = 33.9 bits (76), Expect = 0.16
Identities = 25/77 (32%), Positives = 32/77 (41%), Gaps = 3/77 (3%)
Frame = +1
Query: 372 STPQTQVFK---DGSTVPLEVAKKTEQIVEMLKRLPNNQLLVTVIKRCLTLDFLPQSAKA 428
+TP + FK S E A ++ +K+L N CL L AK
Sbjct: 151 TTPDNEAFKLIPCASAAKDENASVSQSCCAQVKKLGQNP-------SCLCAVMLSNVAKM 309
Query: 429 SGVNPVSLVTVRKRCEL 445
SG NP VT+ KRC L
Sbjct: 310 SGANPQIAVTIPKRCNL 360
>BF645370 weakly similar to GP|12321864|gb| RING zinc finger protein;
17145-17621 {Arabidopsis thaliana}, partial (48%)
Length = 654
Score = 33.1 bits (74), Expect = 0.28
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 2/30 (6%)
Frame = +1
Query: 46 CCVCLGG--RVENLFKCSGCDRLFHADCLD 73
CC+CLG E L GC+ FH DC+D
Sbjct: 409 CCICLGEFRDGEKLKVLPGCEHYFHCDCVD 498
>TC76831 similar to GP|21593924|gb|AAM65889.1 unknown {Arabidopsis
thaliana}, partial (75%)
Length = 692
Score = 32.3 bits (72), Expect = 0.48
Identities = 25/75 (33%), Positives = 31/75 (41%), Gaps = 3/75 (4%)
Frame = +1
Query: 372 STPQTQVFK---DGSTVPLEVAKKTEQIVEMLKRLPNNQLLVTVIKRCLTLDFLPQSAKA 428
+TP + FK S E A ++ K+L N CL L AK
Sbjct: 133 TTPDMEAFKLAPCASAAQDENASVSQTCCAQTKKLGQNP-------SCLCAVLLSNVAKM 291
Query: 429 SGVNPVSLVTVRKRC 443
SGVNP VT+ KRC
Sbjct: 292 SGVNPQIAVTIPKRC 336
>TC84304 similar to GP|4105798|gb|AAD02556.1| PGPD14 {Petunia x hybrida},
partial (75%)
Length = 676
Score = 32.0 bits (71), Expect = 0.62
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +2
Query: 20 CSQEKFWVLQDLLSEDAAPQHYPKDTCCVCLGGRVENLFKCSGCD 64
CS+ KF+ +D + Y D C +C G EN F C+ C+
Sbjct: 278 CSKCKFF------DDDVSKNQYHCDECGICRTGGEENFFHCNKCE 394
>TC93550 weakly similar to GP|20260434|gb|AAM13115.1 putative PHD-type zinc
finger protein {Arabidopsis thaliana}, partial (10%)
Length = 911
Score = 31.2 bits (69), Expect = 1.1
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +3
Query: 52 GRVENLFKCSGCDRLFH 68
G VENLF C GC R FH
Sbjct: 795 GMVENLFLCDGCPRAFH 845
>BQ139867
Length = 595
Score = 31.2 bits (69), Expect = 1.1
Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 5/64 (7%)
Frame = +3
Query: 63 CDRLF-HADCLDVKENEVPNRNWYCLMCIC----SKQLLVLQSYCNSQRKDDAKKNRKVS 117
C RL +A +D N WYCL CIC ++ L + +RK + +R
Sbjct: 9 CARLLRYAIYIDQASPGSDNXYWYCLACICN*LIARVLSLFVPAATRRRKQFDRSDRTSR 188
Query: 118 KDDS 121
+DDS
Sbjct: 189 RDDS 200
>TC87819 similar to GP|16209696|gb|AAL14405.1 AT5g20880/F22D1_50
{Arabidopsis thaliana}, partial (28%)
Length = 1121
Score = 31.2 bits (69), Expect = 1.1
Identities = 17/54 (31%), Positives = 27/54 (49%), Gaps = 7/54 (12%)
Frame = +1
Query: 43 KDTCCVCLGG--RVENLFKCSGCDRLFHADCLDV-----KENEVPNRNWYCLMC 89
++TC VCL + + + CD +FH +C+D +NE N N C +C
Sbjct: 526 EETCAVCLNKLKMEDEVRELMNCDHVFHKECIDKWLEHGHDNE--NHNQTCPLC 681
>BQ143956 similar to GP|21592534|gb| unknown {Arabidopsis thaliana}, partial
(55%)
Length = 763
Score = 30.8 bits (68), Expect = 1.4
Identities = 15/30 (50%), Positives = 16/30 (53%)
Frame = +1
Query: 416 CLTLDFLPQSAKASGVNPVSLVTVRKRCEL 445
CL L AK SG NP VT+ KRC L
Sbjct: 79 CLCAVMLSNVAKMSGANPQIAVTIPKRCNL 168
>TC86960 similar to PIR|C85253|C85253 receptor like protein (partial)
[imported] - Arabidopsis thaliana, partial (80%)
Length = 1023
Score = 30.0 bits (66), Expect = 2.4
Identities = 16/63 (25%), Positives = 29/63 (45%), Gaps = 4/63 (6%)
Frame = +3
Query: 57 LFKCSGCDRLFHADCLDVKENEVPN-RNWYCLMCICSKQLLVLQSYCNSQRKD---DAKK 112
+ +C GC FH C+D+ E +++C C Q + S+ ++ D D K+
Sbjct: 492 MVQCEGCSDWFHPACIDMTVEEAERLDHFFCESCSVEGQKQLQNSHSATRLADTKVDTKR 671
Query: 113 NRK 115
R+
Sbjct: 672 RRR 680
>BG645953 similar to PIR|B96625|B96 hypothetical protein T2K10.12 [imported]
- Arabidopsis thaliana, partial (29%)
Length = 757
Score = 30.0 bits (66), Expect = 2.4
Identities = 17/66 (25%), Positives = 35/66 (52%), Gaps = 1/66 (1%)
Frame = +1
Query: 252 GDKFVQSSVEGRFCDTAIS-VREAALELVGRHIASHPDVGFKYFEKITERIKDTGVSVRK 310
G + + ++ GRF + +R AL ++ R + + ++ I E +KD S+RK
Sbjct: 40 GLRVLAINILGRFLSNRDNNIRYVALNMLMRAVTADAQAVQRHRATILECVKDLDASIRK 219
Query: 311 RAIKII 316
RA++++
Sbjct: 220 RALELV 237
>BE202843 weakly similar to GP|10177512|dbj RING zinc finger protein-like
{Arabidopsis thaliana}, partial (10%)
Length = 260
Score = 29.6 bits (65), Expect = 3.1
Identities = 13/29 (44%), Positives = 16/29 (54%), Gaps = 2/29 (6%)
Frame = +3
Query: 46 CCVCLGGRV--ENLFKCSGCDRLFHADCL 72
CCVCLG E L + C +FH DC+
Sbjct: 147 CCVCLGEFEVKEELLQIPYCKHVFHIDCI 233
>TC77980 similar to PIR|T12970|T12970 hypothetical protein T6H20.190 -
Arabidopsis thaliana, partial (42%)
Length = 1061
Score = 29.6 bits (65), Expect = 3.1
Identities = 19/53 (35%), Positives = 28/53 (51%)
Frame = +2
Query: 396 IVEMLKRLPNNQLLVTVIKRCLTLDFLPQSAKASGVNPVSLVTVRKRCELMCK 448
I E L+++ + T+IK CLT DF P+S+ N V L + R E + K
Sbjct: 860 IQEFLQKVVETDVKYTLIKTCLTDDFAPESS----YNVVVLXEXKHRFERL*K 1006
>TC86319 similar to GP|15451110|gb|AAK96826.1 Unknown protein {Arabidopsis
thaliana}, partial (79%)
Length = 760
Score = 29.3 bits (64), Expect = 4.0
Identities = 14/37 (37%), Positives = 19/37 (50%)
Frame = +3
Query: 409 LVTVIKRCLTLDFLPQSAKASGVNPVSLVTVRKRCEL 445
L+ RCL L AK + +NP +TV KRC +
Sbjct: 249 LLRTSPRCLCSVLLSPLAKQAKINPAIAITVPKRCNI 359
>TC77981 similar to PIR|T12970|T12970 hypothetical protein T6H20.190 -
Arabidopsis thaliana, partial (39%)
Length = 1191
Score = 29.3 bits (64), Expect = 4.0
Identities = 13/31 (41%), Positives = 20/31 (63%)
Frame = +1
Query: 396 IVEMLKRLPNNQLLVTVIKRCLTLDFLPQSA 426
I E L+++ + T+IK CLT DF P+S+
Sbjct: 190 IQEFLQKVVETDVKYTLIKTCLTDDFAPESS 282
>BF634100 similar to GP|18377636|gb| unknown protein {Arabidopsis
thaliana}, partial (14%)
Length = 376
Score = 29.3 bits (64), Expect = 4.0
Identities = 21/68 (30%), Positives = 28/68 (40%), Gaps = 1/68 (1%)
Frame = +2
Query: 7 GTIAARLKRDAVICSQEKFWVLQDLLSEDAAPQHYPKDTCCVCLGGRVEN-LFKCSGCDR 65
GT+AA +++ VI EDA CC+CL N + C
Sbjct: 26 GTVAAGTEKERVISG------------EDAV--------CCICLAKYENNDELRELPCSH 145
Query: 66 LFHADCLD 73
LFH DC+D
Sbjct: 146LFHKDCVD 169
>TC86318 similar to GP|15451110|gb|AAK96826.1 Unknown protein {Arabidopsis
thaliana}, partial (79%)
Length = 618
Score = 29.3 bits (64), Expect = 4.0
Identities = 14/37 (37%), Positives = 19/37 (50%)
Frame = +2
Query: 409 LVTVIKRCLTLDFLPQSAKASGVNPVSLVTVRKRCEL 445
L+ RCL L AK + +NP +TV KRC +
Sbjct: 260 LLRTSPRCLCSVLLSPLAKQAKINPAIAITVPKRCNI 370
>TC81289 weakly similar to PIR|G96791|G96791 probable RING zinc finger
protein 5217-5774 [imported] - Arabidopsis thaliana,
partial (25%)
Length = 616
Score = 29.3 bits (64), Expect = 4.0
Identities = 17/43 (39%), Positives = 20/43 (45%), Gaps = 2/43 (4%)
Frame = +1
Query: 34 EDAAPQHYPKDTCCVCLGG--RVENLFKCSGCDRLFHADCLDV 74
+DA P DTC VCLG E L C FH C+D+
Sbjct: 259 DDAVPDG-EGDTCAVCLGDFEEGEELRTMPECLHSFHVSCIDM 384
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.327 0.139 0.433
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,035,002
Number of Sequences: 36976
Number of extensions: 324567
Number of successful extensions: 2560
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 2513
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2556
length of query: 543
length of database: 9,014,727
effective HSP length: 101
effective length of query: 442
effective length of database: 5,280,151
effective search space: 2333826742
effective search space used: 2333826742
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC149305.6