BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149210.5 + phase: 0
(128 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC91324 47 3e-06
TC88305 weakly similar to GP|19920188|gb|AAM08620.1 Putative Glu... 29 0.57
TC82340 similar to GP|10178240|dbj|BAB11672. gene_id:MDJ22.8~unk... 29 0.57
TC81245 similar to GP|15809986|gb|AAL06920.1 AT3g19540/T31J18_4 ... 28 0.97
TC82370 similar to GP|15028377|gb|AAK76665.1 putative heat shock... 27 1.7
TC78943 weakly similar to GP|20259311|gb|AAM14391.1 unknown prot... 27 1.7
TC92689 similar to PIR|T48090|T48090 hypothetical protein T20O10... 27 1.7
BG588806 weakly similar to GP|19920188|g Putative Glucan 1 3-bet... 27 2.2
TC77066 weakly similar to GP|4115549|dbj|BAA36416.1 lectin-relat... 27 2.2
TC91204 similar to GP|15624006|dbj|BAB68060. putative PGPD14 pro... 27 2.8
CA921430 similar to GP|17978924|gb AT5g45290/K9E15_7 {Arabidopsi... 27 2.8
AW127462 weakly similar to GP|10177406|db cytochrome P-450-like ... 27 2.8
TC78558 similar to PIR|T09914|T09914 protein-arginine N-methyltr... 27 2.8
TC85805 weakly similar to SP|P35334|PGIP_PHAVU Polygalacturonase... 26 3.7
TC80435 similar to GP|12040650|gb|AAB41023.2 phototropin-like pr... 26 3.7
TC81273 similar to PIR|T14278|T14278 myosin-like protein my4 - c... 26 4.8
TC85394 homologue to GP|2642238|gb|AAB86942.1| endoplasmic retic... 26 4.8
TC89549 similar to PIR|F96595|F96595 unknown protein 25817-2483... 26 4.8
TC80987 similar to GP|15809830|gb|AAL06843.1 At2g36310/F2H17.8 {... 26 4.8
BG453800 similar to GP|22267587|gb putative methionine aminopept... 25 6.3
>TC91324
Length = 940
Score = 46.6 bits (109), Expect = 3e-06
Identities = 28/81 (34%), Positives = 45/81 (54%)
Frame = +1
Query: 29 VVQSPSKVEVPDGWLVEQRPRPSNPNHIDRYYIEPHTGQKFRSLVSVQRYLNGGETGDYL 88
+V S S +P GW V+ + R + H D++Y P + QKF S+V V RYL+ + L
Sbjct: 79 MVHSNSDACLPPGWTVKVKVRKNG--HKDKFYFPPSSDQKFNSMVGVLRYLDKAKNKASL 252
Query: 89 PTQRIISENNNNVSSNFTFTS 109
+ IS N+ +V+ TF++
Sbjct: 253 QS---ISNNSEDVTMKPTFSA 306
Score = 38.9 bits (89), Expect = 6e-04
Identities = 18/46 (39%), Positives = 27/46 (58%)
Frame = +1
Query: 38 VPDGWLVEQRPRPSNPNHIDRYYIEPHTGQKFRSLVSVQRYLNGGE 83
+P GW V+ R + H D+YY P + +KF S+V V RYL+ +
Sbjct: 553 LPTGWTVKVNVRKNG--HKDKYYFPPSSEKKFNSMVGVLRYLDNAK 684
>TC88305 weakly similar to GP|19920188|gb|AAM08620.1 Putative Glucan 1
3-beta-glucosidase precursor {Oryza sativa (japonica
cultivar-group)}, partial (15%)
Length = 1003
Score = 28.9 bits (63), Expect = 0.57
Identities = 17/53 (32%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = +3
Query: 2 NRSMDVEMEHLNLNRNVMNLREKELQIVVQSP-SKVEVPDGWLVEQRPRPSNP 53
NR+ V EH NL + E + + + Q+ V +P GW + Q P P P
Sbjct: 615 NRAPQVMREHWNLY-----ITEDDFRFMSQNGLDAVRIPVGWWIAQDPNPPKP 758
>TC82340 similar to GP|10178240|dbj|BAB11672. gene_id:MDJ22.8~unknown
protein {Arabidopsis thaliana}, partial (9%)
Length = 641
Score = 28.9 bits (63), Expect = 0.57
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +1
Query: 98 NNNVSSNFTFTSFCCIIRLYSC 119
+ N+S N F +FCC+ +LYSC
Sbjct: 418 DGNISGN-VFLNFCCVPQLYSC 480
>TC81245 similar to GP|15809986|gb|AAL06920.1 AT3g19540/T31J18_4
{Arabidopsis thaliana}, partial (20%)
Length = 753
Score = 28.1 bits (61), Expect = 0.97
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +2
Query: 71 SLVSVQRYLNGGETGDYLPTQRIISENNNNVSSNFTFT 108
SL+ Y NG ETG L TQR S S +F+F+
Sbjct: 254 SLIFFNYYYNGKETGFLLCTQRRSSSRPLTFSHSFSFS 367
>TC82370 similar to GP|15028377|gb|AAK76665.1 putative heat shock factor
protein hsf8 {Arabidopsis thaliana}, partial (7%)
Length = 645
Score = 27.3 bits (59), Expect = 1.7
Identities = 14/29 (48%), Positives = 17/29 (58%)
Frame = -1
Query: 98 NNNVSSNFTFTSFCCIIRLYSCFCFNLYD 126
NN +S NF+FT CI+R F NL D
Sbjct: 513 NNVMSLNFSFTCPLCILREKFGFILNLED 427
>TC78943 weakly similar to GP|20259311|gb|AAM14391.1 unknown protein
{Arabidopsis thaliana}, partial (41%)
Length = 1258
Score = 27.3 bits (59), Expect = 1.7
Identities = 18/60 (30%), Positives = 27/60 (45%)
Frame = -2
Query: 63 PHTGQKFRSLVSVQRYLNGGETGDYLPTQRIISENNNNVSSNFTFTSFCCIIRLYSCFCF 122
PH QK +S + RYL G G + Q+ I ++ NF +C + +C CF
Sbjct: 417 PHHDQKAKSYLEWGRYLAGRNGGSSI--QKYILDH-----WNFVS*MYCTWTTIQNCLCF 259
>TC92689 similar to PIR|T48090|T48090 hypothetical protein T20O10.110 -
Arabidopsis thaliana, partial (9%)
Length = 587
Score = 27.3 bits (59), Expect = 1.7
Identities = 10/20 (50%), Positives = 14/20 (70%), Gaps = 3/20 (15%)
Frame = +1
Query: 112 CIIRLYSC---FCFNLYDGN 128
C++ + SC FCFN+Y GN
Sbjct: 202 CVVIILSCSITFCFNIYGGN 261
>BG588806 weakly similar to GP|19920188|g Putative Glucan 1
3-beta-glucosidase precursor {Oryza sativa (japonica
cultivar-group)}, partial (9%)
Length = 791
Score = 26.9 bits (58), Expect = 2.2
Identities = 16/53 (30%), Positives = 24/53 (45%), Gaps = 1/53 (1%)
Frame = +1
Query: 2 NRSMDVEMEHLNLNRNVMNLREKELQIVVQSP-SKVEVPDGWLVEQRPRPSNP 53
NR+ V EH N + E + + + Q+ V +P GW + Q P P P
Sbjct: 544 NRAPQVMREHWN-----SYITEDDFRFMSQNGLDAVRIPVGWWIAQDPNPPKP 687
>TC77066 weakly similar to GP|4115549|dbj|BAA36416.1 lectin-related
polypeptide {Robinia pseudoacacia}, partial (45%)
Length = 1105
Score = 26.9 bits (58), Expect = 2.2
Identities = 21/70 (30%), Positives = 32/70 (45%), Gaps = 6/70 (8%)
Frame = +3
Query: 56 IDRYYIEPH---TGQKFRSLVSVQRYLNGGETGDYL-PTQRIISENNNNV--SSNFTFTS 109
+D Y+ PH G S V ++ +L PT++IIS+N NNV S+
Sbjct: 702 LDLSYLLPHEVVVGISATSGVRQSHFIYSWSFTSFLDPTKKIISDNINNVIASN*IRMHD 881
Query: 110 FCCIIRLYSC 119
F C++ C
Sbjct: 882 FICMVYCIVC 911
>TC91204 similar to GP|15624006|dbj|BAB68060. putative PGPD14 protein
(pollen germination related protein) {Oryza sativa
(japonica cultivar-group), partial (29%)
Length = 1238
Score = 26.6 bits (57), Expect = 2.8
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +1
Query: 107 FTSFCCIIRLYSCFCFNL 124
F+SF C+ RLY C C L
Sbjct: 52 FSSF*CLARLYFCSCIKL 105
>CA921430 similar to GP|17978924|gb AT5g45290/K9E15_7 {Arabidopsis thaliana},
partial (20%)
Length = 585
Score = 26.6 bits (57), Expect = 2.8
Identities = 10/24 (41%), Positives = 13/24 (53%)
Frame = -2
Query: 98 NNNVSSNFTFTSFCCIIRLYSCFC 121
N++ S F F FC R+ SC C
Sbjct: 410 NSSAISGFIFPPFCIFYRICSCTC 339
>AW127462 weakly similar to GP|10177406|db cytochrome P-450-like protein
{Arabidopsis thaliana}, partial (21%)
Length = 407
Score = 26.6 bits (57), Expect = 2.8
Identities = 12/23 (52%), Positives = 16/23 (69%), Gaps = 2/23 (8%)
Frame = -2
Query: 107 FTSFCCIIRLY--SCFCFNLYDG 127
FT+F CI L+ +CFC+NL G
Sbjct: 382 FTNFPCIFILHTATCFCYNLSCG 314
>TC78558 similar to PIR|T09914|T09914 protein-arginine N-methyltransferase
(EC 2.1.1.23) - Arabidopsis thaliana, partial (83%)
Length = 1348
Score = 26.6 bits (57), Expect = 2.8
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +1
Query: 106 TFTSFCCIIRLYSCFC 121
TF + CC R YSCFC
Sbjct: 832 TF*ARCCS*RFYSCFC 879
>TC85805 weakly similar to SP|P35334|PGIP_PHAVU Polygalacturonase inhibitor
precursor (Polygalacturonase-inhibiting protein).,
partial (26%)
Length = 922
Score = 26.2 bits (56), Expect = 3.7
Identities = 18/46 (39%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
Frame = +1
Query: 83 ETGDYLPTQRIISENNNNVSSNFTFTSFCCIIRLYS-CFCFNLYDG 127
E GD L + I NNN + TF CI+ L++ CF F L+ G
Sbjct: 649 EGGD*LSST*ISVFNNNLS*HSSTFFFLVCIVLLFNMCFYFILFMG 786
>TC80435 similar to GP|12040650|gb|AAB41023.2 phototropin-like protein PsPK4
{Pisum sativum}, partial (5%)
Length = 554
Score = 26.2 bits (56), Expect = 3.7
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = -2
Query: 111 CCIIRLYSCFCFNLYDG 127
CC+I CFC++ Y G
Sbjct: 217 CCMITTMYCFCWHFYKG 167
>TC81273 similar to PIR|T14278|T14278 myosin-like protein my4 - common
sunflower, partial (22%)
Length = 1078
Score = 25.8 bits (55), Expect = 4.8
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +2
Query: 112 CIIRLYSCFCFN 123
C IRL+ CFCFN
Sbjct: 107 CQIRLHYCFCFN 142
>TC85394 homologue to GP|2642238|gb|AAB86942.1| endoplasmic reticulum
HSC70-cognate binding protein precursor {Glycine max},
partial (38%)
Length = 962
Score = 25.8 bits (55), Expect = 4.8
Identities = 22/62 (35%), Positives = 31/62 (49%), Gaps = 2/62 (3%)
Frame = +3
Query: 2 NRSMDVEMEHLNLNR-NVMNLREKELQIVVQSPSKVEVP-DGWLVEQRPRPSNPNHIDRY 59
NRS HL L+R N+ + E+E + K+E DGWLVE R + +H +R
Sbjct: 81 NRSHSHHHSHLQLHRFNLRSCSEEESR-----ERKLERENDGWLVETRLSFCSCHHFNRM 245
Query: 60 YI 61
I
Sbjct: 246 SI 251
>TC89549 similar to PIR|F96595|F96595 unknown protein 25817-24837
[imported] - Arabidopsis thaliana, partial (6%)
Length = 857
Score = 25.8 bits (55), Expect = 4.8
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +1
Query: 93 IISENNNNVSSNFTFTSFCCIIRL 116
I++ N+V + F SFCCI+++
Sbjct: 151 IVNHQVNSVQKSAFFGSFCCILKI 222
>TC80987 similar to GP|15809830|gb|AAL06843.1 At2g36310/F2H17.8 {Arabidopsis
thaliana}, partial (76%)
Length = 1089
Score = 25.8 bits (55), Expect = 4.8
Identities = 10/28 (35%), Positives = 13/28 (45%)
Frame = -2
Query: 94 ISENNNNVSSNFTFTSFCCIIRLYSCFC 121
I NNNN+ C + +Y CFC
Sbjct: 896 IYSNNNNICPRGKHNICCFWVSIYICFC 813
>BG453800 similar to GP|22267587|gb putative methionine aminopeptidase {Oryza
sativa (japonica cultivar-group)}, partial (35%)
Length = 672
Score = 25.4 bits (54), Expect = 6.3
Identities = 9/24 (37%), Positives = 14/24 (57%)
Frame = -3
Query: 101 VSSNFTFTSFCCIIRLYSCFCFNL 124
+S NF + F + +SC CF+L
Sbjct: 121 LSLNFEYQGFMIFVVYHSCICFSL 50
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.137 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,671,385
Number of Sequences: 36976
Number of extensions: 65471
Number of successful extensions: 687
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 680
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 686
length of query: 128
length of database: 9,014,727
effective HSP length: 85
effective length of query: 43
effective length of database: 5,871,767
effective search space: 252485981
effective search space used: 252485981
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)
Medicago: description of AC149210.5