BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149134.10 + phase: 0
(478 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC91469 weakly similar to GP|5923668|gb|AAD56319.1| hypothetical... 380 e-119
TC85529 similar to GP|9758042|dbj|BAB08505.1 gb|AAD56319.1~gene_... 216 2e-56
AW587108 similar to PIR|H86313|H863 protein F2H15.10 [imported] ... 174 7e-44
BF649995 similar to PIR|H86313|H863 protein F2H15.10 [imported] ... 164 5e-41
TC85532 similar to GP|9758042|dbj|BAB08505.1 gb|AAD56319.1~gene_... 83 2e-19
BE940956 79 5e-15
TC82853 similar to GP|6503014|gb|AAF14550.1| FH protein interact... 54 1e-07
BF650380 similar to GP|18203774|gb Unknown (protein for MGC:1404... 35 0.083
BF643938 similar to GP|16226415|gb| AT4g30940/F6I18_150 {Arabido... 30 1.6
TC82204 similar to GP|21537142|gb|AAM61483.1 unknown {Arabidopsi... 29 3.5
TC85212 similar to PIR|S44294|S44294 legumin A precursor - sprin... 29 4.5
BG449042 homologue to GP|15004984|db glutamate dehydrogenase {Br... 29 4.5
AW692089 29 4.5
TC91854 similar to GP|9759561|dbj|BAB11163.1 MtN21 nodulin prote... 29 4.5
BE205076 similar to EGAD|59173|6188 85 kDa calcium-independent p... 28 7.7
>TC91469 weakly similar to GP|5923668|gb|AAD56319.1| hypothetical protein
{Arabidopsis thaliana}, partial (37%)
Length = 703
Score = 380 bits (977), Expect(2) = e-119
Identities = 191/197 (96%), Positives = 192/197 (96%)
Frame = +2
Query: 1 MEEESTSQTTTDSNVTDRIKLNVGGKLFETTLSTIRSGGPDSLLFALSNRFSNDPNPVFI 60
MEEESTSQTTTDSNVTDRIKLNVGGKLFETTLSTIRSGGPDSLLFALSNRFSNDPNPVFI
Sbjct: 47 MEEESTSQTTTDSNVTDRIKLNVGGKLFETTLSTIRSGGPDSLLFALSNRFSNDPNPVFI 226
Query: 61 DRDPEIFSVFLSLLRTNHLPSTARRFSKQELADEALFYGIDSHLRNATSPPPFSGIDASI 120
DRDPEIFSVFLSLLRTNHLPSTARRFSKQELADEALFYGIDSHLRNATSPPPFSGIDASI
Sbjct: 227 DRDPEIFSVFLSLLRTNHLPSTARRFSKQELADEALFYGIDSHLRNATSPPPFSGIDASI 406
Query: 121 VASVRPASEGLSSTFTAAENGSVWIAHGGQLSSYDWNLIHSATVRTHLDEINSICRVWPE 180
VASVRPASEGLSSTFTAAENGSVWIAHGGQLSSYDWNLIHSATVRTHLDEINSICRVWPE
Sbjct: 407 VASVRPASEGLSSTFTAAENGSVWIAHGGQLSSYDWNLIHSATVRTHLDEINSICRVWPE 586
Query: 181 IAAVGTESDAGLHFYNF 197
IAAVGTESDA + F
Sbjct: 587 IAAVGTESDAWITLLQF 637
Score = 66.6 bits (161), Expect(2) = e-119
Identities = 28/28 (100%), Positives = 28/28 (100%)
Frame = +3
Query: 191 GLHFYNFSGSRHVGSVHWSDPSDPRIFK 218
GLHFYNFSGSRHVGSVHWSDPSDPRIFK
Sbjct: 618 GLHFYNFSGSRHVGSVHWSDPSDPRIFK 701
>TC85529 similar to GP|9758042|dbj|BAB08505.1
gb|AAD56319.1~gene_id:MYC6.4~similar to unknown protein
{Arabidopsis thaliana}, partial (68%)
Length = 1818
Score = 216 bits (549), Expect = 2e-56
Identities = 139/461 (30%), Positives = 235/461 (50%), Gaps = 7/461 (1%)
Frame = +1
Query: 16 TDRIKLNVGGKLFETTLSTIRSGGPDSLLFALSNRFSNDPNPVFIDRDPEIFSVFLSLLR 75
T+ + ++VGG++F+TT T+ S GP + +S ++ P FIDRDP+IFS+ LSLLR
Sbjct: 184 TNIVTIDVGGQIFQTTKQTLTSAGPKTFFSTISQ--TSSPYTPFIDRDPDIFSLLLSLLR 357
Query: 76 TNHLPSTARRFSKQELADEALFYGIDSHLRNATS-PPPFSGIDASIVASVRPASEGLSST 134
T +LPS A+ F Q+L E+ FYGI++ L N+ S P F + + ST
Sbjct: 358 TGNLPSKAKSFDLQDLIIESKFYGIENLLINSLSNPSQFEPFNLQNSLQLPLNGRDSPST 537
Query: 135 FTAAENGSVWIAHGGQLSSYDWNLIHSATVRTHLDEINSICRVWPEIAAVGTESDAGLHF 194
+ GS+ +AHG +++++DW+L +T+ TH ++S+ + P +AA G +GL
Sbjct: 538 VSTTPYGSLHVAHGSKITTFDWSLRRKSTILTHFTAVDSLLALSPSLAAAGANDFSGLQI 717
Query: 195 YNFSGSRHVGSVHWSDPSDPRIFKARVNAITASDNSVFASFDCPHR-ENCILEVDKAKLQ 253
+ ++ W + + + V AI +S ++F SF+ R N IL D L+
Sbjct: 718 LDLENGYVKDTLIWENVTKS---GSTVQAIGSSPENMFVSFESSRRNSNSILVYDLHSLR 888
Query: 254 IVSQLGRQS--GNQAKNMVPG-KLTFVPTTGVLVGSAVTGGAFGYSGYIRVWDPRSGEVV 310
V+++ G + +P KL ++ + +L+ S G G SG +R+WD RSG VV
Sbjct: 889 PVTEIAHNEIYGADIDSAIPATKLQWIESHNLLMASGSHSGPSGVSGNVRLWDVRSGNVV 1068
Query: 311 WETNEPGAGRSSRFGDSFSAIDVDVEKLLLFKLCSKSGDLAMADMRRLGDD--PWVYLKE 368
WE +E D F+ + V +FK+ SG+ D++ L + WV L +
Sbjct: 1069WEISEK--------VDCFADVTVSDSLDAMFKVGVNSGEAFYMDLKNLSSEKNSWVCLGD 1224
Query: 369 KNPSLWTDGGDGSTSSVVNCYKGQVFVGRGGELEVWSRVKEVVECESERDRESDGEGVYR 428
K L +G + QVF +GG++E+WS + S +++
Sbjct: 1225KRKVL--NGKKEGFGCKIETQGNQVFCTKGGDVELWSDI----------IMGSSNNRIFK 1368
Query: 429 RNFVDKREDSEKGVIVKIEGGGDRLFISREDVEGIEVWESS 469
+N + + +D I + GG R+F++R+D++ +EVW+SS
Sbjct: 1369KNLMGRVQDMGGAKITNLAFGGSRMFLTRKDLQCVEVWQSS 1491
>AW587108 similar to PIR|H86313|H863 protein F2H15.10 [imported] -
Arabidopsis thaliana, partial (9%)
Length = 647
Score = 174 bits (441), Expect = 7e-44
Identities = 83/97 (85%), Positives = 88/97 (90%)
Frame = +2
Query: 92 ADEALFYGIDSHLRNATSPPPFSGIDASIVASVRPASEGLSSTFTAAENGSVWIAHGGQL 151
+D+ALFYGID+HLRNATSPPPFSGIDASIV SVRPASEGLSS FTA ENGS WIA GGQL
Sbjct: 11 SDKALFYGIDNHLRNATSPPPFSGIDASIVVSVRPASEGLSSAFTATENGSTWIAIGGQL 190
Query: 152 SSYDWNLIHSATVRTHLDEINSICRVWPEIAAVGTES 188
SYDWNLIHS +VR+HLDEINSICRVWPE AA GTES
Sbjct: 191 PSYDWNLIHSVSVRSHLDEINSICRVWPESAAAGTES 301
>BF649995 similar to PIR|H86313|H863 protein F2H15.10 [imported] -
Arabidopsis thaliana, partial (9%)
Length = 540
Score = 164 bits (416), Expect = 5e-41
Identities = 79/88 (89%), Positives = 81/88 (91%)
Frame = +2
Query: 101 DSHLRNATSPPPFSGIDASIVASVRPASEGLSSTFTAAENGSVWIAHGGQLSSYDWNLIH 160
D+HLRNATSPPPFSGIDASIV SVRPASEGLSSTFTA ENGS WIA GGQLSSYDWNLIH
Sbjct: 2 DNHLRNATSPPPFSGIDASIVVSVRPASEGLSSTFTATENGSTWIAIGGQLSSYDWNLIH 181
Query: 161 SATVRTHLDEINSICRVWPEIAAVGTES 188
S TVR+HLDEINSICRVWPE AA GTES
Sbjct: 182 SVTVRSHLDEINSICRVWPESAAAGTES 265
>TC85532 similar to GP|9758042|dbj|BAB08505.1
gb|AAD56319.1~gene_id:MYC6.4~similar to unknown protein
{Arabidopsis thaliana}, partial (43%)
Length = 742
Score = 82.8 bits (203), Expect(2) = 2e-19
Identities = 59/204 (28%), Positives = 100/204 (48%), Gaps = 10/204 (4%)
Frame = +2
Query: 77 NHLPSTARRFSKQELADEALFYGIDSHLRNATS-PPPFSGIDASIVASVRPASEGLSSTF 135
N LPS A+ F Q+L E+ FYGI++ L N+ S P F + + ST
Sbjct: 122 NLLPSKAKPFDLQDLIIESKFYGIENLLINSLSNPSQFEPFNLQNSLQLPLNGRDSPSTV 301
Query: 136 TAAENGSVWIAHGGQLSSYDWNLIHSATVRTHLDEINSICRVWPEIAAVGTESDAGLHFY 195
+ GS+ +AHG +++++DW+L +T+ TH ++S+ + P +AA G +GL
Sbjct: 302 STTPYGSLHVAHGSKITTFDWSLRRKSTILTHFTAVDSLLALSPSLAAAGANDFSGLQIL 481
Query: 196 NFSGSRHVGSVHWSDPSDPRIFKARVNAITASDNSVFASFDCPHR-ENCILEVDKAKLQI 254
+ ++ W + + + V AI +S ++F SF+ R N IL D L+
Sbjct: 482 DLENGYVKDTLIWENVTKS---GSTVQAIGSSPENMFVSFESSRRNSNSILVYDLHSLRP 652
Query: 255 VSQL------GRQS--GNQAKNMV 270
V+++ GR+S GN N +
Sbjct: 653 VTEIAHNEIYGRRSYIGNCYSNFI 724
Score = 31.2 bits (69), Expect(2) = 2e-19
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = +3
Query: 16 TDRIKLNVGGKLFETTLSTIRSGGPDSLLFALSN 49
T+ + ++VGG++F+TT T+ S GP + N
Sbjct: 45 TNIVTIDVGGQIFQTTKQTLTSAGPKTFFLQKQN 146
>BE940956
Length = 397
Score = 78.6 bits (192), Expect = 5e-15
Identities = 38/42 (90%), Positives = 39/42 (92%)
Frame = +1
Query: 437 DSEKGVIVKIEGGGDRLFISREDVEGIEVWESSHYAGAISVL 478
+ E GVIVKIEGGGDRLFI REDVEGIEVWESSHYAGAISVL
Sbjct: 1 ERENGVIVKIEGGGDRLFIIREDVEGIEVWESSHYAGAISVL 126
>TC82853 similar to GP|6503014|gb|AAF14550.1| FH protein interacting protein
FIP2 {Arabidopsis thaliana}, partial (87%)
Length = 878
Score = 54.3 bits (129), Expect = 1e-07
Identities = 35/91 (38%), Positives = 51/91 (55%), Gaps = 5/91 (5%)
Frame = +3
Query: 13 SNVTDRIKLNVGGKLFETTLSTIRSGGPDSLLFAL---SNRFSNDPNP--VFIDRDPEIF 67
S+++ ++LN+GGK F TT+ T+ PDS+L A+ + S DPN VF+DRD + F
Sbjct: 81 SDLSSALRLNIGGKKFWTTIDTVTQREPDSMLAAMFSGRHTLSQDPNNGYVFVDRDGKHF 260
Query: 68 SVFLSLLRTNHLPSTARRFSKQELADEALFY 98
L+ LR +P T EL EA +Y
Sbjct: 261 RHILNWLRDGVVP-TLEEAEYTELLREAEYY 350
>BF650380 similar to GP|18203774|gb Unknown (protein for MGC:14049) {Mus
musculus}, partial (1%)
Length = 664
Score = 34.7 bits (78), Expect = 0.083
Identities = 15/58 (25%), Positives = 27/58 (45%), Gaps = 1/58 (1%)
Frame = +1
Query: 148 GGQLSSYDWNLIHSATVRTHLDEINSICRVWPE-IAAVGTESDAGLHFYNFSGSRHVG 204
G +++ +D H A VR H D + + WP+ I + + + LH + G +G
Sbjct: 436 GQEVAGFDQACFHVAAVRGHTDVVRELLNKWPDLIQVIDEKGNTALHHAXYKGHFEIG 609
>BF643938 similar to GP|16226415|gb| AT4g30940/F6I18_150 {Arabidopsis
thaliana}, partial (5%)
Length = 356
Score = 30.4 bits (67), Expect = 1.6
Identities = 11/21 (52%), Positives = 17/21 (80%)
Frame = +1
Query: 15 VTDRIKLNVGGKLFETTLSTI 35
+ DR+K NV G++FETT +T+
Sbjct: 289 INDRVKFNVRGRVFETTATTL 351
>TC82204 similar to GP|21537142|gb|AAM61483.1 unknown {Arabidopsis
thaliana}, partial (15%)
Length = 522
Score = 29.3 bits (64), Expect = 3.5
Identities = 23/80 (28%), Positives = 32/80 (39%), Gaps = 1/80 (1%)
Frame = +1
Query: 152 SSYDWNLIHSATVRTHLDEINSICRVWPEIAAVGTESDAGLHFYNFSGSRHVGSVH-WSD 210
S D N H A R HLD + I WP + + +S Y + H+ V+ D
Sbjct: 286 SKSDMNAFHVAAKRGHLDIVREILSAWPAVCKL-CDSTNTSPLYAAAVQDHLDGVNAILD 462
Query: 211 PSDPRIFKARVNAITASDNS 230
+F R N TA N+
Sbjct: 463 VDVSSMFIVRKNGKTALHNA 522
>TC85212 similar to PIR|S44294|S44294 legumin A precursor - spring vetch,
partial (90%)
Length = 1769
Score = 28.9 bits (63), Expect = 4.5
Identities = 18/38 (47%), Positives = 22/38 (57%), Gaps = 4/38 (10%)
Frame = +3
Query: 416 ERDRESDGEGVYRRNFVDK----REDSEKGVIVKIEGG 449
+RD D V R+ V+K ED EKG IVK+EGG
Sbjct: 798 KRDFLEDALNV-NRHIVEKLQGRNEDQEKGAIVKVEGG 908
>BG449042 homologue to GP|15004984|db glutamate dehydrogenase {Brassica
napus}, partial (43%)
Length = 577
Score = 28.9 bits (63), Expect = 4.5
Identities = 19/62 (30%), Positives = 29/62 (46%)
Frame = -3
Query: 100 IDSHLRNATSPPPFSGIDASIVASVRPASEGLSSTFTAAENGSVWIAHGGQLSSYDWNLI 159
I SH+R P ++A+ V ++ E S TF + +W+A L S WN+
Sbjct: 482 ISSHIRGRNIP-----MNANEVMNL--LGENTSETF*LTDAQILWVAANSALCSSVWNVY 324
Query: 160 HS 161
HS
Sbjct: 323 HS 318
>AW692089
Length = 524
Score = 28.9 bits (63), Expect = 4.5
Identities = 13/32 (40%), Positives = 20/32 (61%), Gaps = 2/32 (6%)
Frame = -1
Query: 131 LSSTFTAAENGSVW--IAHGGQLSSYDWNLIH 160
L S+F+ EN + IAH ++ S+DW L+H
Sbjct: 296 LESSFSGDENSVMLSLIAHSKKILSFDWQLVH 201
>TC91854 similar to GP|9759561|dbj|BAB11163.1 MtN21 nodulin protein-like
{Arabidopsis thaliana}, partial (20%)
Length = 400
Score = 28.9 bits (63), Expect = 4.5
Identities = 11/27 (40%), Positives = 15/27 (54%)
Frame = -2
Query: 361 DPWVYLKEKNPSLWTDGGDGSTSSVVN 387
DPW YL KN S W++ + S+ N
Sbjct: 306 DPWPYLPFKNNSKWSNNSSSKSMSINN 226
>BE205076 similar to EGAD|59173|6188 85 kDa calcium-independent phospholipase
A2 {Mus musculus}, partial (2%)
Length = 549
Score = 28.1 bits (61), Expect = 7.7
Identities = 15/52 (28%), Positives = 25/52 (47%), Gaps = 1/52 (1%)
Frame = +1
Query: 151 LSSYDWNLIHSATVRTHLDEINSICRVWPEIAAVGTES-DAGLHFYNFSGSR 201
L+ +D H A R H D + + WP+++ V E+ ++ LH G R
Sbjct: 289 LAGFDQACFHIAASRGHTDIVRELLNRWPDLSQVIDENGNSALHHACNKGHR 444
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.134 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,699,769
Number of Sequences: 36976
Number of extensions: 210308
Number of successful extensions: 893
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 879
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 886
length of query: 478
length of database: 9,014,727
effective HSP length: 100
effective length of query: 378
effective length of database: 5,317,127
effective search space: 2009874006
effective search space used: 2009874006
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC149134.10