BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149130.3 - phase: 0 /pseudo
(171 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI272761 72 1e-13
BE998730 66 6e-12
BG646118 57 5e-09
BI308165 54 2e-08
CA921015 homologue to SP|Q8Y7G1|DPO3 DNA polymerase III polC-typ... 54 4e-08
TC89815 52 2e-07
AW256529 homologue to GP|496669|emb|C C-169 protein {Saccharomyc... 51 2e-07
TC84316 weakly similar to GP|11994612|dbj|BAB02749. gb|AAC24186.... 50 4e-07
TC81783 similar to GP|9294656|dbj|BAB03005.1 gb|AAF30317.1~gene_... 46 8e-06
TC89353 weakly similar to GP|9279704|dbj|BAB01261.1 gb|AAF25964.... 46 8e-06
BI265054 44 3e-05
TC84654 43 7e-05
TC83420 weakly similar to GP|5903055|gb|AAD55614.1| May be a pse... 42 1e-04
BG646382 42 1e-04
TC93564 40 4e-04
TC91177 similar to GP|9294656|dbj|BAB03005.1 gb|AAF30317.1~gene_... 40 6e-04
TC91633 40 6e-04
CA920710 similar to PIR|S64314|S643 probable membrane protein YG... 39 8e-04
TC90324 weakly similar to PIR|D85256|D85256 hypothetical protein... 39 0.001
CA922988 homologue to GP|21114266|gb| citrate-dependent iron tra... 38 0.002
>BI272761
Length = 600
Score = 71.6 bits (174), Expect = 1e-13
Identities = 38/85 (44%), Positives = 51/85 (59%)
Frame = +2
Query: 1 MGVFVENSIRCIMAKKLDGLHPSLIVAFNLTLEIFNEVPLPAELQGETSLLIDVAVLGGC 60
MGVFV ++ ++ + L+ LIVAF+L E+F EV LP + G+ + DVA+L G
Sbjct: 305 MGVFVSGALHWVVTRDLEPESRDLIVAFDLRFEVFREVALPGTVDGKFDM--DVALLXGM 478
Query: 61 LCITVNYETTKIDVWVMKEYGYRDS 85
LCI N + DVWVM EYG DS
Sbjct: 479 LCIIENRGSDGFDVWVMXEYGSHDS 553
>BE998730
Length = 820
Score = 66.2 bits (160), Expect = 6e-12
Identities = 32/42 (76%), Positives = 36/42 (85%)
Frame = +1
Query: 2 GVFVENSIRCIMAKKLDGLHPSLIVAFNLTLEIFNEVPLPAE 43
GVFV+NS+ +MAKKLDG +P LIVAFNLTLEIF EVPLP E
Sbjct: 655 GVFVQNSLHWVMAKKLDGSYPWLIVAFNLTLEIFYEVPLPVE 780
>BG646118
Length = 764
Score = 56.6 bits (135), Expect = 5e-09
Identities = 33/93 (35%), Positives = 50/93 (53%), Gaps = 1/93 (1%)
Frame = -1
Query: 1 MGVFVENSIRCIMAKKLDGLHPSLIVAFNLTLEIFNEVPLPAELQGET-SLLIDVAVLGG 59
+G V +I ++A++ D IV+ +L E EV LP E+ T L + VL
Sbjct: 716 LGKSVSGTINWLVARQYDNKFQDCIVSLDLGSESCKEVLLPKEVDTSTLRLRWYLGVLRD 537
Query: 60 CLCITVNYETTKIDVWVMKEYGYRDSWCKLFTL 92
CLC+ + DVW+MKE+G DSW KL+++
Sbjct: 536 CLCLVFGH-----DVWIMKEHGNNDSWAKLYSV 453
>BI308165
Length = 462
Score = 54.3 bits (129), Expect = 2e-08
Identities = 31/84 (36%), Positives = 45/84 (52%), Gaps = 5/84 (5%)
Frame = -3
Query: 35 FNEVPLPAELQGE---TSLLIDVAVLGGCLCITVNYETTKIDVWVMKEYGYRDSWCKLFT 91
F VP P+ E I V VL GCLC+ E +++ WVM +YG ++SW K F+
Sbjct: 442 FKSVPPPSHFDDEYVSRISTISVGVLKGCLCLCYVIEGARLETWVMSKYGEKESWSKAFS 263
Query: 92 L-VKS-CFTSHLRYSNPIGYSSDG 113
+ +KS C S PIG+++ G
Sbjct: 262 IEIKSYCGLSPQDKHRPIGFNTFG 191
>CA921015 homologue to SP|Q8Y7G1|DPO3 DNA polymerase III polC-type (EC
2.7.7.7) (PolIII). {Listeria monocytogenes}, partial
(0%)
Length = 824
Score = 53.5 bits (127), Expect = 4e-08
Identities = 44/145 (30%), Positives = 71/145 (48%)
Frame = -1
Query: 2 GVFVENSIRCIMAKKLDGLHPSLIVAFNLTLEIFNEVPLPAELQGETSLLIDVAVLGGCL 61
GVFV ++ + + S I++ ++ E + +V LP E L I + VL CL
Sbjct: 689 GVFVSGTVNWLASDD------SFILSLDVEKESYQQVLLP---DTENDLWI-LGVLRNCL 540
Query: 62 CITVNYETTKIDVWVMKEYGYRDSWCKLFTLVKSCFTSHLRYSNPIGYSSDGSKVLFEGI 121
CI +DVW+M EYG ++SW KL+++ H + + YSS+ ++L E
Sbjct: 539 CILAT-SNLFLDVWIMNEYGNQESWTKLYSVPN--MQDHGLEAYTVLYSSEDDQLLLEFN 369
Query: 122 EVLLDVHYKKLFWYDLKSERVSYVE 146
E+ D KL YD K+ ++ E
Sbjct: 368 EMRSD--KVKLVVYDSKTGTLNIPE 300
>TC89815
Length = 807
Score = 51.6 bits (122), Expect = 2e-07
Identities = 27/46 (58%), Positives = 32/46 (68%), Gaps = 7/46 (15%)
Frame = +1
Query: 1 MGVFVE-------NSIRCIMAKKLDGLHPSLIVAFNLTLEIFNEVP 39
MGVFVE NS+ ++ +KL+ P LIVAFNLTLEIFNEVP
Sbjct: 622 MGVFVEDSNNLNSNSLHWVVTRKLEPFQPDLIVAFNLTLEIFNEVP 759
>AW256529 homologue to GP|496669|emb|C C-169 protein {Saccharomyces
cerevisiae}, partial (7%)
Length = 624
Score = 51.2 bits (121), Expect = 2e-07
Identities = 28/92 (30%), Positives = 50/92 (53%), Gaps = 1/92 (1%)
Frame = +1
Query: 2 GVFVENSIRCIMAKKLDGLHP-SLIVAFNLTLEIFNEVPLPAELQGETSLLIDVAVLGGC 60
G+FV ++ + LD + IV+ +L EI+ E+ P G+ ++ + + + C
Sbjct: 46 GIFVSGTVNWLADYDLDDNNSLGTIVSLDLRKEIYQEISQPD--YGDVTVKLSLGAMREC 219
Query: 61 LCITVNYETTKIDVWVMKEYGYRDSWCKLFTL 92
LC+ ++ + DVW+MKEYG +SW KL +
Sbjct: 220 LCV-FSHSDSFDDVWLMKEYGNEESWIKLIRI 312
>TC84316 weakly similar to GP|11994612|dbj|BAB02749.
gb|AAC24186.1~gene_id:MGL6.4~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (10%)
Length = 892
Score = 50.1 bits (118), Expect = 4e-07
Identities = 27/92 (29%), Positives = 52/92 (56%)
Frame = +3
Query: 1 MGVFVENSIRCIMAKKLDGLHPSLIVAFNLTLEIFNEVPLPAELQGETSLLIDVAVLGGC 60
+G FV ++ I+ ++ DG + I++F++ E + +V LP G + VL C
Sbjct: 603 LGKFVSGTLNWIVDER-DGR--ATILSFDIEKETYRQVLLPQH--GYAVYSPGLYVLSNC 767
Query: 61 LCITVNYETTKIDVWVMKEYGYRDSWCKLFTL 92
+C+ ++ T+ +W+MK+YG +SW KL ++
Sbjct: 768 ICVCTSFLDTRWQLWMMKKYGVAESWTKLMSI 863
>TC81783 similar to GP|9294656|dbj|BAB03005.1
gb|AAF30317.1~gene_id:F14O13.6~similar to unknown
protein {Arabidopsis thaliana}, partial (6%)
Length = 1225
Score = 45.8 bits (107), Expect = 8e-06
Identities = 37/110 (33%), Positives = 59/110 (53%), Gaps = 3/110 (2%)
Frame = +2
Query: 52 IDVAVLGGCLCITVNYETTKI--DVWVMKEYGYRDSWCKLFTLVKSCFTSHLRY-SNPIG 108
+ V +L CLC+ YE + + +VW+MKEYG ++SW KL+++ K H R+ + I
Sbjct: 863 LSVLMLRDCLCV---YEASDLFFNVWIMKEYGNQESWTKLYSVPK---MQHRRFKAYTIS 1024
Query: 109 YSSDGSKVLFEGIEVLLDVHYKKLFWYDLKSERVSYVEGIPKLFFFKKPM 158
Y + ++L GI V + KL YD K+ ++ E F+KPM
Sbjct: 1025YIYEDDQLLL-GI-VDMQSSNTKLAVYDSKTGTLNIPE-------FEKPM 1147
>TC89353 weakly similar to GP|9279704|dbj|BAB01261.1
gb|AAF25964.1~gene_id:MYA6.2~similar to unknown protein
{Arabidopsis thaliana}, partial (10%)
Length = 1482
Score = 45.8 bits (107), Expect = 8e-06
Identities = 37/141 (26%), Positives = 68/141 (47%), Gaps = 1/141 (0%)
Frame = +3
Query: 1 MGVFVENSIRCIMAKKLDGLHPSLIVAFNLTLEIFNEVPLPAELQGETSLLIDVAVLGGC 60
+G FV ++ I++KK+ IV F++ E + E+ LP + + ++L V
Sbjct: 702 VGKFVSGNLNWIVSKKV-------IVFFDIEKETYGEMSLPQDYGDKNTVLY---VSSNR 851
Query: 61 LCITVNYET-TKIDVWVMKEYGYRDSWCKLFTLVKSCFTSHLRYSNPIGYSSDGSKVLFE 119
+ ++ ++ T VW+MKEYG +SW KL + + TS P Y + + E
Sbjct: 852 IYVSFDHSNKTHWVVWMMKEYGVVESWTKLMIIPQDKLTS------PGPYCLSDALFISE 1013
Query: 120 GIEVLLDVHYKKLFWYDLKSE 140
+L+ + KL Y+L ++
Sbjct: 1014HGVLLMRPQHSKLSVYNLNND 1076
>BI265054
Length = 679
Score = 43.9 bits (102), Expect = 3e-05
Identities = 39/142 (27%), Positives = 64/142 (44%), Gaps = 12/142 (8%)
Frame = -1
Query: 25 IVAFNLTLEIFNEVPLPAE----LQGETSLLIDVAVLGGCLCITVNYETTKIDVWVMKEY 80
+V+F L E+F P+P++ + + S + V + G I + ETT D+ ++ E
Sbjct: 529 LVSFYLRNEVFCITPIPSDEDDCFKFKASSINLVLLNGSISLIAFHRETTTFDIAILGEL 350
Query: 81 GYRDSWCKLFTL-VKSCFTSHLRYSNP---IGYSSDGSKVLFE-GIEVLLDVHYKKLFWY 135
G ++SW KLFT+ SC +R D F+ +V+ ++ YK Y
Sbjct: 349 GIKESWTKLFTVGPLSCVERPIRVGTKGEIFFQRKDNELAWFDLSTQVIEELGYKARGPY 170
Query: 136 ---DLKSERVSYVEGIPKLFFF 154
L E + +EGI FF
Sbjct: 169 TRISLYKENILAIEGISN*LFF 104
>TC84654
Length = 605
Score = 42.7 bits (99), Expect = 7e-05
Identities = 25/71 (35%), Positives = 40/71 (56%), Gaps = 2/71 (2%)
Frame = +1
Query: 22 PSLIVAFNLTLEIFNEVPLP--AELQGETSLLIDVAVLGGCLCITVNYETTKIDVWVMKE 79
P++IV+ +L E + + P E+ E + + VL C+C+ + T DVWVMKE
Sbjct: 7 PAVIVSLDLENESYRALLFPDFGEMNVEA---LTLEVLMDCMCLLCHSGTFS-DVWVMKE 174
Query: 80 YGYRDSWCKLF 90
+G +SW +LF
Sbjct: 175FGNENSWARLF 207
>TC83420 weakly similar to GP|5903055|gb|AAD55614.1| May be a pseudogene of
F6D8.29. {Arabidopsis thaliana}, partial (10%)
Length = 1210
Score = 42.0 bits (97), Expect = 1e-04
Identities = 25/70 (35%), Positives = 38/70 (53%), Gaps = 2/70 (2%)
Frame = +1
Query: 25 IVAFNLTLEIFNEVPLPA-ELQGETSLLIDVAVLGGCLCITVNYETTK-IDVWVMKEYGY 82
+ +F+ T EIF E LP+ +L+ S D+AVL G + N T + +W++ E
Sbjct: 880 MASFDFTNEIFFETTLPSFDLKDGMSTKKDLAVLHGSVAFIHNVVNTGYLHIWILGELAV 1059
Query: 83 RDSWCKLFTL 92
+SW KLF L
Sbjct: 1060NESWTKLFVL 1089
>BG646382
Length = 603
Score = 42.0 bits (97), Expect = 1e-04
Identities = 18/53 (33%), Positives = 32/53 (59%)
Frame = +3
Query: 40 LPAELQGETSLLIDVAVLGGCLCITVNYETTKIDVWVMKEYGYRDSWCKLFTL 92
LPA + + + VL CLC + +++ T+ +W MKE+G ++SW +LF +
Sbjct: 195 LPAGFKEVPCVEPSLRVLMDCLCFSHDFKRTEFVIWQMKEFGSQESWTQLFRI 353
>TC93564
Length = 476
Score = 40.4 bits (93), Expect = 4e-04
Identities = 24/94 (25%), Positives = 41/94 (43%), Gaps = 1/94 (1%)
Frame = +1
Query: 54 VAVLGGCLCITVNYETTKI-DVWVMKEYGYRDSWCKLFTLVKSCFTSHLRYSNPIGYSSD 112
+AV+ G + N E D+W++ E G ++SW KLF + C P G
Sbjct: 88 LAVIDGSIGFISNIEVLSFYDIWILGEVGVKESWIKLFVVDAPC------KMRPFGIGIK 249
Query: 113 GSKVLFEGIEVLLDVHYKKLFWYDLKSERVSYVE 146
+G E ++ WYDL ++R+ ++
Sbjct: 250 SFIFFIKGDE--------EIAWYDLSTQRIEEIK 327
>TC91177 similar to GP|9294656|dbj|BAB03005.1
gb|AAF30317.1~gene_id:F14O13.6~similar to unknown
protein {Arabidopsis thaliana}, partial (9%)
Length = 1298
Score = 39.7 bits (91), Expect = 6e-04
Identities = 23/70 (32%), Positives = 35/70 (49%), Gaps = 1/70 (1%)
Frame = +2
Query: 24 LIVAFNLTLEIFNEVPLPAELQGETSLLIDVAVLGGCLCIT-VNYETTKIDVWVMKEYGY 82
+IVAF+L E+ LP E+ S D+ V GCL + + + +WVM++Y
Sbjct: 728 VIVAFHLMERKLLELRLPNEIINGPSRAYDLWVYRGCLALWHILPDRVTFQIWVMEKYNV 907
Query: 83 RDSWCKLFTL 92
+ SW K L
Sbjct: 908 QSSWTKTLVL 937
>TC91633
Length = 711
Score = 39.7 bits (91), Expect = 6e-04
Identities = 29/83 (34%), Positives = 41/83 (48%)
Frame = +2
Query: 25 IVAFNLTLEIFNEVPLPAELQGETSLLIDVAVLGGCLCITVNYETTKIDVWVMKEYGYRD 84
I+AF+L E+P P E + D+ + G L + T I++WVMKEY
Sbjct: 2 IIAFDLVERELLEMPFPDGFDHEP-MDCDLWIFGEFLSLWAMGGVT-IEIWVMKEYKVHS 175
Query: 85 SWCKLFTLVKSCFTSHLRYSNPI 107
SW K TLV S +++Y PI
Sbjct: 176 SWTK--TLVLSIDYIYIQYDPPI 238
>CA920710 similar to PIR|S64314|S643 probable membrane protein YGR023w -
yeast (Saccharomyces cerevisiae), partial (7%)
Length = 774
Score = 39.3 bits (90), Expect = 8e-04
Identities = 16/37 (43%), Positives = 23/37 (61%)
Frame = -2
Query: 56 VLGGCLCITVNYETTKIDVWVMKEYGYRDSWCKLFTL 92
VL CLC + T+ +W MKE+G R+SW +LF +
Sbjct: 749 VLLDCLCFLHDLGKTEFVIWQMKEFGVRESWTRLFKI 639
>TC90324 weakly similar to PIR|D85256|D85256 hypothetical protein AT4g22390
[imported] - Arabidopsis thaliana, partial (5%)
Length = 657
Score = 38.9 bits (89), Expect = 0.001
Identities = 28/99 (28%), Positives = 44/99 (44%)
Frame = +1
Query: 53 DVAVLGGCLCITVNYETTKIDVWVMKEYGYRDSWCKLFTLVKSCFTSHLRYSNPIGYSSD 112
++ VL CLCI DVWV+KE G ++SW K +++ Y+ + Y S+
Sbjct: 49 NLGVLKDCLCIYTR-NIMFFDVWVLKECGNKESWTKFYSVPYMEDQGSFSYTKAL-YISE 222
Query: 113 GSKVLFEGIEVLLDVHYKKLFWYDLKSERVSYVEGIPKL 151
++L + + DL S V V IPK+
Sbjct: 223 DDRILVD--------------FRDLGSNEVKLVVYIPKM 297
>CA922988 homologue to GP|21114266|gb| citrate-dependent iron transporter
{Xanthomonas campestris pv. campestris str. ATCC 33913},
partial (1%)
Length = 786
Score = 38.1 bits (87), Expect = 0.002
Identities = 33/108 (30%), Positives = 54/108 (49%), Gaps = 1/108 (0%)
Frame = -2
Query: 40 LPAELQGETSLLIDVAVLGGCLCITVNYETTKIDVWVMKEYGYRDSWCKLFTLVKSCFTS 99
LP +L+ + L + ++ LCI + E +DVW+M+EYG ++SW KL C
Sbjct: 770 LPPDLETD---LWTLGLVWDYLCIFASNELF-MDVWIMEEYGKKESWTKL------CKVP 621
Query: 100 HLRYSNPIGYSSDGSKVLFEGIEVLLDVHYK-KLFWYDLKSERVSYVE 146
+L +P + + L E +V+LD KL YD K++ + E
Sbjct: 620 YLEDQSPRLRAFYKAFYLSEDGQVVLDACVPLKLAVYDSKTDALKIRE 477
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.143 0.444
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,165,084
Number of Sequences: 36976
Number of extensions: 88938
Number of successful extensions: 658
Number of sequences better than 10.0: 72
Number of HSP's better than 10.0 without gapping: 654
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 655
length of query: 171
length of database: 9,014,727
effective HSP length: 89
effective length of query: 82
effective length of database: 5,723,863
effective search space: 469356766
effective search space used: 469356766
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC149130.3