BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149129.2 + phase: 0
(261 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC88735 similar to GP|21536589|gb|AAM60921.1 unknown {Arabidopsi... 267 4e-80
TC89694 similar to GP|20197031|gb|AAC27479.2 expressed protein {... 220 4e-58
BI272323 similar to GP|15810014|gb| At2g44770/F16B22.26 {Arabido... 207 4e-54
BI263795 similar to GP|21536589|gb| unknown {Arabidopsis thalian... 182 9e-47
TC90437 similar to GP|18377969|gb|AAL67127.1 unknown protein {Ar... 116 1e-32
AI974500 weakly similar to GP|9828622|gb|A F1N21.22 {Arabidopsis... 94 5e-20
BE319306 similar to GP|9828622|gb| F1N21.22 {Arabidopsis thalian... 39 0.002
TC79419 weakly similar to GP|10177901|dbj|BAB11233. 6-phosphoglu... 28 3.6
BI271514 similar to GP|9857294|dbj CpABA1 {Vigna unguiculata}, p... 27 6.1
BG453284 homologue to SP|Q9XHE4|R37A_G 60S ribosomal protein L37... 27 7.9
BQ752185 similar to SP|Q12731|TF2D_ Transcription initiation fac... 27 7.9
TC82422 similar to GP|15983392|gb|AAL11564.1 At1g59990/T2K10_4 {... 27 7.9
TC76857 homologue to SP|Q9XHE4|R37A_GOSHI 60S ribosomal protein ... 27 7.9
>TC88735 similar to GP|21536589|gb|AAM60921.1 unknown {Arabidopsis
thaliana}, partial (63%)
Length = 967
Score = 267 bits (683), Expect(2) = 4e-80
Identities = 129/145 (88%), Positives = 133/145 (90%)
Frame = +3
Query: 1 MDDRGGSFVAVRRISQGLDRGNAYHSSSAEFVTGSTAWIGRGLSCVCAQRRESDARLSFD 60
MDDRGGSFVAVRRISQGLDRGNAYHSSSAEFVTGSTAWIGRGLSCVCAQRRESDARLSFD
Sbjct: 453 MDDRGGSFVAVRRISQGLDRGNAYHSSSAEFVTGSTAWIGRGLSCVCAQRRESDARLSFD 632
Query: 61 LTPYQEECLQRLQSRIDVPYDSSIPEHQASLRALWNAAFPEEELNGLISEQWKDMGWQGK 120
LTPYQEECLQRLQSRIDVPYDSSIPEHQASLRALWNAAFPE++LNGLISEQWKDMGWQGK
Sbjct: 633 LTPYQEECLQRLQSRIDVPYDSSIPEHQASLRALWNAAFPEQKLNGLISEQWKDMGWQGK 812
Query: 121 DPSTDFRGGGYISLENLLFFARNFP 145
DPSTDFRG + F + FP
Sbjct: 813 DPSTDFRGWWLHIIGEFAVFCQEFP 887
Score = 48.1 bits (113), Expect(2) = 4e-80
Identities = 22/24 (91%), Positives = 23/24 (95%)
Frame = +2
Query: 144 FPIFCGSRKEIVQCGSTRLRLLVL 167
F IFCGSRKEIVQCGSTRLR+LVL
Sbjct: 896 FKIFCGSRKEIVQCGSTRLRVLVL 967
Score = 41.6 bits (96), Expect = 3e-04
Identities = 18/18 (100%), Positives = 18/18 (100%)
Frame = +1
Query: 128 GGGYISLENLLFFARNFP 145
GGGYISLENLLFFARNFP
Sbjct: 835 GGGYISLENLLFFARNFP 888
>TC89694 similar to GP|20197031|gb|AAC27479.2 expressed protein {Arabidopsis
thaliana}, partial (93%)
Length = 1134
Score = 220 bits (561), Expect = 4e-58
Identities = 109/145 (75%), Positives = 119/145 (81%)
Frame = +3
Query: 1 MDDRGGSFVAVRRISQGLDRGNAYHSSSAEFVTGSTAWIGRGLSCVCAQRRESDARLSFD 60
MDDRG SFVAVRR + D N S+SAE V GS AW+GRGLSCVCAQRR+SDA FD
Sbjct: 207 MDDRGASFVAVRRPNPHTDTCN---SNSAEAVAGSAAWLGRGLSCVCAQRRDSDAGSFFD 377
Query: 61 LTPYQEECLQRLQSRIDVPYDSSIPEHQASLRALWNAAFPEEELNGLISEQWKDMGWQGK 120
LT QEECL+RLQ RI+VPYD SI EHQ +L+ALW AFPEEEL GLISEQWK+MGWQGK
Sbjct: 378 LTLAQEECLERLQRRIEVPYDGSIVEHQEALKALWKVAFPEEELRGLISEQWKEMGWQGK 557
Query: 121 DPSTDFRGGGYISLENLLFFARNFP 145
DPSTDFRGGG+ISLEN LFFARNFP
Sbjct: 558 DPSTDFRGGGFISLENFLFFARNFP 632
Score = 139 bits (349), Expect = 1e-33
Identities = 79/127 (62%), Positives = 90/127 (70%), Gaps = 1/127 (0%)
Frame = +1
Query: 136 NLLFFARNFPIFC-GSRKEIVQCGSTRLRLLVLTLHSCLFKC*ILKQLSHGHWWEQLS*N 194
+LL +RN C GS KEIV CGSTRL LLVLT HSC +C ILKQ SH HWWEQL *+
Sbjct: 613 SLLGISRNPSRICYGSGKEIVPCGSTRLPLLVLTSHSCWCRCLILKQSSHAHWWEQLF*S 792
Query: 195 SLQKMSRPLIFSIA*HSS*WTINGFPCTRHTWILIQ**NLHAVSWRKNFSLKTLHN*KMY 254
L+KM++ LIFS A* SS*W NGF C HTWI IQ**NLH S +K+F K L K+Y
Sbjct: 793 FLKKMNQLLIFSTA*LSS*WITNGFLCVPHTWISIQ**NLHVKS*KKSFFKKFLVL-KIY 969
Query: 255 PHTNFSH 261
PHTN+ H
Sbjct: 970 PHTNYFH 990
>BI272323 similar to GP|15810014|gb| At2g44770/F16B22.26 {Arabidopsis
thaliana}, partial (37%)
Length = 687
Score = 207 bits (526), Expect = 4e-54
Identities = 100/100 (100%), Positives = 100/100 (100%)
Frame = +2
Query: 162 LRLLVLTLHSCLFKC*ILKQLSHGHWWEQLS*NSLQKMSRPLIFSIA*HSS*WTINGFPC 221
LRLLVLTLHSCLFKC*ILKQLSHGHWWEQLS*NSLQKMSRPLIFSIA*HSS*WTINGFPC
Sbjct: 11 LRLLVLTLHSCLFKC*ILKQLSHGHWWEQLS*NSLQKMSRPLIFSIA*HSS*WTINGFPC 190
Query: 222 TRHTWILIQ**NLHAVSWRKNFSLKTLHN*KMYPHTNFSH 261
TRHTWILIQ**NLHAVSWRKNFSLKTLHN*KMYPHTNFSH
Sbjct: 191 TRHTWILIQ**NLHAVSWRKNFSLKTLHN*KMYPHTNFSH 310
>BI263795 similar to GP|21536589|gb| unknown {Arabidopsis thaliana}, partial
(37%)
Length = 666
Score = 182 bits (463), Expect = 9e-47
Identities = 92/104 (88%), Positives = 92/104 (88%)
Frame = +1
Query: 1 MDDRGGSFVAVRRISQGLDRGNAYHSSSAEFVTGSTAWIGRGLSCVCAQRRESDARLSFD 60
MDDRGGSFVAVRRISQGLDRGNAYHSSS GSTAWIGRGLSCVCAQRRESDARLSFD
Sbjct: 370 MDDRGGSFVAVRRISQGLDRGNAYHSSS-----GSTAWIGRGLSCVCAQRRESDARLSFD 534
Query: 61 LTPYQEECLQRLQSRIDVPYDSSIPEHQASLRALWNAAFPEEEL 104
LTPYQEECL RL S IDVPYD SIPEH ASLRALWNAAFPE L
Sbjct: 535 LTPYQEECLXRLXSXIDVPYDXSIPEHXASLRALWNAAFPEXXL 666
>TC90437 similar to GP|18377969|gb|AAL67127.1 unknown protein {Arabidopsis
thaliana}, partial (81%)
Length = 1148
Score = 116 bits (291), Expect(2) = 1e-32
Identities = 58/119 (48%), Positives = 82/119 (68%), Gaps = 1/119 (0%)
Frame = +3
Query: 29 AEFVTGSTAWIGRGLSCVCAQ-RRESDARLSFDLTPYQEECLQRLQSRIDVPYDSSIPEH 87
A V GS + +GR LS + +++ + L+P QEE L+ L+ R++VP+D S +H
Sbjct: 219 ANIVLGSGSLLGRLLSHSSSGFYLQNNRMVPPTLSPVQEERLRNLKQRLEVPFDGSKTDH 398
Query: 88 QASLRALWNAAFPEEELNGLISEQWKDMGWQGKDPSTDFRGGGYISLENLLFFARNFPI 146
Q +L LW A+P+ EL L S+ WK+MGWQG DPSTDFRGGG+ISLENL+FFA+ +P+
Sbjct: 399 QDALLQLWKLAYPDRELPPLKSDCWKEMGWQGSDPSTDFRGGGFISLENLIFFAQKYPV 575
Score = 40.0 bits (92), Expect(2) = 1e-32
Identities = 33/105 (31%), Positives = 47/105 (44%)
Frame = +1
Query: 150 SRKEIVQCGSTRLRLLVLTLHSCLFKC*ILKQLSHGHWWEQLS*NSLQKMSRPLIFSIA* 209
++ E Q G+ L+ L H C +C I KQL H E + L++M LIF A
Sbjct: 598 NKMESEQSGNIHLQ*LESISHLCWCRCWIFKQLFHLLRQEYVFYGCLRRMKWHLIFFFAL 777
Query: 210 HSS*WTINGFPCTRHTWILIQ**NLHAVSWRKNFSLKTLHN*KMY 254
*W ++G H W L *+ + + LKT *K+Y
Sbjct: 778 PFK*WMLSG*QSVLHIWNLTMF*SQQEYN*SASLLLKTPPV*KIY 912
>AI974500 weakly similar to GP|9828622|gb|A F1N21.22 {Arabidopsis thaliana},
partial (24%)
Length = 284
Score = 94.0 bits (232), Expect = 5e-20
Identities = 44/86 (51%), Positives = 63/86 (73%)
Frame = +1
Query: 34 GSTAWIGRGLSCVCAQRRESDARLSFDLTPYQEECLQRLQSRIDVPYDSSIPEHQASLRA 93
GS +G+GL+CVC + + + + + TP QEE L+RL+ R+ V +D+S +HQ +LRA
Sbjct: 25 GSP*GMGKGLTCVCFKPKGNCQPICINSTPLQEERLRRLKRRMKVYFDASKLDHQDALRA 204
Query: 94 LWNAAFPEEELNGLISEQWKDMGWQG 119
LW+A+FP EL GLIS+QWK+MGWQG
Sbjct: 205 LWSASFPGHELKGLISDQWKEMGWQG 282
>BE319306 similar to GP|9828622|gb| F1N21.22 {Arabidopsis thaliana}, partial
(35%)
Length = 568
Score = 38.9 bits (89), Expect = 0.002
Identities = 16/22 (72%), Positives = 19/22 (85%)
Frame = +2
Query: 125 DFRGGGYISLENLLFFARNFPI 146
DFRG G+ISLENLLFFA+ F +
Sbjct: 2 DFRGAGFISLENLLFFAKTFSV 67
>TC79419 weakly similar to GP|10177901|dbj|BAB11233.
6-phosphogluconolactonase-like protein {Arabidopsis
thaliana}, partial (63%)
Length = 1448
Score = 28.1 bits (61), Expect = 3.6
Identities = 15/34 (44%), Positives = 22/34 (64%), Gaps = 8/34 (23%)
Frame = +3
Query: 162 LRLLVLTLH--------SCLFKC*ILKQLSHGHW 187
++L++ TLH S LFK *ILK++S+G W
Sbjct: 867 VKLMLFTLHLRKVLMIISFLFKR*ILKEISNGSW 968
>BI271514 similar to GP|9857294|dbj CpABA1 {Vigna unguiculata}, partial (30%)
Length = 653
Score = 27.3 bits (59), Expect = 6.1
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = -2
Query: 131 YISLENLLFFARNFPIFC 148
YIS +N LFF+ N+ I+C
Sbjct: 94 YISSQNCLFFSGNYQIYC 41
>BG453284 homologue to SP|Q9XHE4|R37A_G 60S ribosomal protein L37A. [Upland
cotton] {Gossypium hirsutum}, complete
Length = 663
Score = 26.9 bits (58), Expect = 7.9
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -2
Query: 147 FCGSRKEIVQCGSTRLRLLVLTLHSCL 173
+C + + +QC RLRLL+L + C+
Sbjct: 287 WCSEQSQHLQCSKCRLRLLLLYRNPCI 207
>BQ752185 similar to SP|Q12731|TF2D_ Transcription initiation factor TFIID
(TATA-box factor) (TATA sequence-binding protein)
(TBP)., partial (31%)
Length = 659
Score = 26.9 bits (58), Expect = 7.9
Identities = 13/47 (27%), Positives = 21/47 (44%)
Frame = -3
Query: 14 ISQGLDRGNAYHSSSAEFVTGSTAWIGRGLSCVCAQRRESDARLSFD 60
+++G D G + G T W+ G+SCV + R E + D
Sbjct: 648 LARGEDEGGRLGFADTHNDGGKTLWVVLGVSCVQSNRLEIQTAVEVD 508
>TC82422 similar to GP|15983392|gb|AAL11564.1 At1g59990/T2K10_4 {Arabidopsis
thaliana}, partial (43%)
Length = 1093
Score = 26.9 bits (58), Expect = 7.9
Identities = 12/39 (30%), Positives = 20/39 (50%)
Frame = +3
Query: 3 DRGGSFVAVRRISQGLDRGNAYHSSSAEFVTGSTAWIGR 41
D+GG V ++G+D N H A+F T + ++ R
Sbjct: 696 DKGGVLVCTDAAARGVDIPNVSHVIQADFATSAVDFLHR 812
>TC76857 homologue to SP|Q9XHE4|R37A_GOSHI 60S ribosomal protein L37A.
[Upland cotton] {Gossypium hirsutum}, complete
Length = 656
Score = 26.9 bits (58), Expect = 7.9
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -2
Query: 147 FCGSRKEIVQCGSTRLRLLVLTLHSCL 173
+C + + +QC RLRLL+L + C+
Sbjct: 301 WCSEQSQHLQCSKCRLRLLLLYRNPCI 221
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.337 0.145 0.501
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,090,928
Number of Sequences: 36976
Number of extensions: 163792
Number of successful extensions: 1040
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1031
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1037
length of query: 261
length of database: 9,014,727
effective HSP length: 94
effective length of query: 167
effective length of database: 5,538,983
effective search space: 925010161
effective search space used: 925010161
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC149129.2