BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149127.3 + phase: 0
(218 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC88221 homologue to GP|8809592|dbj|BAA97143.1 gene_id:MNB8.9~pi... 350 e-106
BF640699 similar to GP|23617260|dbj hypothetical protein~predict... 39 0.002
AL375098 36 0.013
TC90992 similar to PIR|F86242|F86242 unknown protein 98896-9585... 33 0.085
TC89597 homologue to GP|17473753|gb|AAL38317.1 unknown protein {... 32 0.25
TC84690 similar to GP|21540004|gb|AAM54368.1 elongation factor 1... 31 0.32
BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xy... 31 0.32
TC80027 similar to GP|15451020|gb|AAK96781.1 Unknown protein {Ar... 30 0.55
TC93461 29 1.2
TC92840 similar to PIR|D85354|D85354 hypothetical protein AT4g30... 29 1.2
BQ144332 similar to GP|398168|emb|C keratin 2 epidermis {Mus mus... 29 1.6
TC88229 similar to PIR|E84471|E84471 probable beta-1 3-glucanase... 29 1.6
BF519564 29 1.6
TC86006 weakly similar to GP|15146334|gb|AAK83650.1 At1g07080/F1... 28 2.1
TC87643 similar to PIR|C71405|C71405 probable casein kinase I - ... 28 2.1
CA920109 similar to GP|22535636|dbj hypothetical protein~predict... 28 2.7
BQ150627 28 2.7
TC84341 homologue to GP|48188|emb|CAA77863.1|| N-terminus of bet... 28 2.7
BQ158066 similar to GP|15293967|gb| polyprotein {Potato virus Y}... 28 2.7
TC81827 similar to GP|9758799|dbj|BAB09252.1 serine/threonine pr... 28 3.6
>TC88221 homologue to GP|8809592|dbj|BAA97143.1
gene_id:MNB8.9~pir||T09020~similar to unknown protein
{Arabidopsis thaliana}, partial (8%)
Length = 1080
Score = 350 bits (898), Expect(2) = e-106
Identities = 168/168 (100%), Positives = 168/168 (100%)
Frame = +3
Query: 1 MTSENNKKPDHDDELRSNYDTPKKLSLPTILTRPGFSSEAPTPPPRTTVISIPFKWEEAP 60
MTSENNKKPDHDDELRSNYDTPKKLSLPTILTRPGFSSEAPTPPPRTTVISIPFKWEEAP
Sbjct: 132 MTSENNKKPDHDDELRSNYDTPKKLSLPTILTRPGFSSEAPTPPPRTTVISIPFKWEEAP 311
Query: 61 GKPRSCHTRPELREREVNNVVRALELPPRLLSLERESTGNIYAPSPTTVLEGPYVGRAVS 120
GKPRSCHTRPELREREVNNVVRALELPPRLLSLERESTGNIYAPSPTTVLEGPYVGRAVS
Sbjct: 312 GKPRSCHTRPELREREVNNVVRALELPPRLLSLERESTGNIYAPSPTTVLEGPYVGRAVS 491
Query: 121 FTTSYRDNNNKDSVNFGSSRWGGLKKNNRIDREGSFDFSSWSVEGGDK 168
FTTSYRDNNNKDSVNFGSSRWGGLKKNNRIDREGSFDFSSWSVEGGDK
Sbjct: 492 FTTSYRDNNNKDSVNFGSSRWGGLKKNNRIDREGSFDFSSWSVEGGDK 635
Score = 50.8 bits (120), Expect(2) = e-106
Identities = 21/28 (75%), Positives = 24/28 (85%)
Frame = +1
Query: 168 KVKITRVKRRGSFSHGTSHFWVCLFSYF 195
+VKITRVKRRGSFSHGTSHFW ++ F
Sbjct: 634 RVKITRVKRRGSFSHGTSHFWASIYGSF 717
>BF640699 similar to GP|23617260|dbj hypothetical protein~predicted by
FGENESH etc. {Oryza sativa (japonica cultivar-group)},
partial (8%)
Length = 444
Score = 38.9 bits (89), Expect = 0.002
Identities = 39/138 (28%), Positives = 63/138 (45%), Gaps = 4/138 (2%)
Frame = +2
Query: 2 TSENNKKPDHDDELRSNYDTPKKLSLPTILTRPGFSSEAPTPPPRTTVISIPFKWEEAPG 61
+SENN K T K +S T L P + S + P ++PF WE +PG
Sbjct: 74 SSENNNKRK----------TEKPVSKRTRL--PAYKSXLKSGPVSNPG-TVPFVWENSPG 214
Query: 62 KPRSCHTRPELREREVNNVVRALELPP-RLLSLERESTGNIYAPSPTTVLE---GPYVGR 117
+P+ +L+ R++ + A +LPP R+L +++ I P T+V + G V
Sbjct: 215 RPKD---EGKLQTRDIEEPLVAPKLPPGRVLKVKQRDFDKI--PKGTSVSQSRTGSTVSN 379
Query: 118 AVSFTTSYRDNNNKDSVN 135
++S + N DS N
Sbjct: 380 SMSVASLESKEENHDSRN 433
>AL375098
Length = 306
Score = 35.8 bits (81), Expect = 0.013
Identities = 22/51 (43%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +3
Query: 146 KNNRIDREGSFDFSSWSVEGGDKVKITRVKRRGS-FSHGTSHFWVCLFSYF 195
K N+ EGSFDFS G +KVK +R + GS S G SH W ++ F
Sbjct: 3 KINKEGDEGSFDFS-----GQNKVKSSRSVKGGSPISKGKSHLWASIYDSF 140
>TC90992 similar to PIR|F86242|F86242 unknown protein 98896-95855
[imported] - Arabidopsis thaliana, partial (14%)
Length = 763
Score = 33.1 bits (74), Expect = 0.085
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +3
Query: 185 SHFWVCLFSYFLFIFILFYISYPFMFHSLFRIVL 218
SH + L S+F F F L Y +PF+F F + L
Sbjct: 87 SHHNISLPSFFFFFFFLLYFKFPFIFQGNFYLCL 188
>TC89597 homologue to GP|17473753|gb|AAL38317.1 unknown protein {Arabidopsis
thaliana}, partial (10%)
Length = 1061
Score = 31.6 bits (70), Expect = 0.25
Identities = 23/80 (28%), Positives = 37/80 (45%), Gaps = 3/80 (3%)
Frame = +1
Query: 17 SNYDTPKKLSLPTILTRPGFSSEAPTPPPRTTVISIPFKWEEAPGKPRSCHTRPELRERE 76
S PKKL P+ S+ P PPPRTT +P + P P + E++ ++
Sbjct: 298 STMPAPKKLVQPS-------SNGIPAPPPRTTPPPLPKRAMPPPLPPPKFYDSIEVKVQD 456
Query: 77 VNNVVRALE---LPPRLLSL 93
+N +R + +P L+ L
Sbjct: 457 KSNSLRKTKSDAIPDTLVKL 516
>TC84690 similar to GP|21540004|gb|AAM54368.1 elongation factor 1-alpha
{Trichophyton rubrum}, partial (24%)
Length = 546
Score = 31.2 bits (69), Expect = 0.32
Identities = 20/65 (30%), Positives = 28/65 (42%)
Frame = +1
Query: 42 TPPPRTTVISIPFKWEEAPGKPRSCHTRPELREREVNNVVRALELPPRLLSLERESTGNI 101
T P R+ ++++P + P P S P R R VR L PP SL +
Sbjct: 1 TTPVRSVLVTLPSLFATLPTLPASS---PRSRRRSTAEPVRLLRPPPSSSSLVTLPSSRW 171
Query: 102 YAPSP 106
+ PSP
Sbjct: 172 FLPSP 186
>BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xylostella
granulovirus}, partial (42%)
Length = 909
Score = 31.2 bits (69), Expect = 0.32
Identities = 21/54 (38%), Positives = 24/54 (43%)
Frame = +3
Query: 17 SNYDTPKKLSLPTILTRPGFSSEAPTPPPRTTVISIPFKWEEAPGKPRSCHTRP 70
+N PKK PT T P PTPPP TT + P EAP P + P
Sbjct: 189 TNTTNPKKQHTPTTPTHPPRPHTPPTPPP-TTQTTPP---REAPTDPGTTRGTP 338
>TC80027 similar to GP|15451020|gb|AAK96781.1 Unknown protein {Arabidopsis
thaliana}, partial (30%)
Length = 926
Score = 30.4 bits (67), Expect = 0.55
Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 4/50 (8%)
Frame = +3
Query: 161 WSVEGGDKVKI-TRVKRRGSFSHGTSHFWV---CLFSYFLFIFILFYISY 206
++ GGD V++ K +G + S+++ CLFS LF+F+ FY+ Y
Sbjct: 726 FAFRGGDHVRLGVYQKVKGLNNQLLSNYYAHASCLFSRRLFLFVFFYLPY 875
>TC93461
Length = 612
Score = 29.3 bits (64), Expect = 1.2
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +3
Query: 186 HFWVCLFSYFLFIFILFYISYPFM 209
H VC FS F+F F L + S PF+
Sbjct: 39 HQLVCPFSLFIFFFYLVFFSNPFL 110
>TC92840 similar to PIR|D85354|D85354 hypothetical protein AT4g30310
[imported] - Arabidopsis thaliana, partial (23%)
Length = 679
Score = 29.3 bits (64), Expect = 1.2
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = -2
Query: 191 LFSYFLFIFILFYISYPFMFHSLFRIVL 218
LFSYF++IF+L+ + F F + I+L
Sbjct: 150 LFSYFVYIFLLYISDFIFRFQWVASIIL 67
>BQ144332 similar to GP|398168|emb|C keratin 2 epidermis {Mus musculus},
partial (7%)
Length = 1163
Score = 28.9 bits (63), Expect = 1.6
Identities = 11/41 (26%), Positives = 22/41 (52%)
Frame = -1
Query: 162 SVEGGDKVKITRVKRRGSFSHGTSHFWVCLFSYFLFIFILF 202
+++G D K + ++ G + W+C + F F+F+LF
Sbjct: 503 NLDGRDSPKALKRRQAGKYGFILYPGWICFWV*FFFLFVLF 381
>TC88229 similar to PIR|E84471|E84471 probable beta-1 3-glucanase [imported]
- Arabidopsis thaliana, partial (69%)
Length = 1368
Score = 28.9 bits (63), Expect = 1.6
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = +2
Query: 4 ENNKKPDHDDELRSNYDTPKKLSLPTILTRPGFSSEAPTPPPRTT 48
EN+ P H + S++ TPK S P+ SS T PP T+
Sbjct: 296 ENSPTPSHGSKKTSSFTTPKPKSKPSPSATK--SSSTHTTPPNTS 424
>BF519564
Length = 473
Score = 28.9 bits (63), Expect = 1.6
Identities = 15/42 (35%), Positives = 20/42 (46%)
Frame = +3
Query: 45 PRTTVISIPFKWEEAPGKPRSCHTRPELREREVNNVVRALEL 86
P + SIPF WEE PG P P + N + +L+L
Sbjct: 3 PLKQIASIPFVWEEKPGIP-----LPNFSHVDENESITSLDL 113
>TC86006 weakly similar to GP|15146334|gb|AAK83650.1 At1g07080/F10K1_15
{Arabidopsis thaliana}, partial (63%)
Length = 1063
Score = 28.5 bits (62), Expect = 2.1
Identities = 19/46 (41%), Positives = 24/46 (51%), Gaps = 3/46 (6%)
Frame = +3
Query: 176 RRGSFSHGTSHFWVC---LFSYFLFIFILFYISYPFMFHSLFRIVL 218
R FSHG ++ L +FLF+F+ F IS S FRIVL
Sbjct: 18 RDSLFSHGFFQHFISFSNLIPFFLFLFLSFSISQ----QSFFRIVL 143
>TC87643 similar to PIR|C71405|C71405 probable casein kinase I - Arabidopsis
thaliana, partial (71%)
Length = 2349
Score = 28.5 bits (62), Expect = 2.1
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +3
Query: 194 YFLFIFILFYISYPFMFHSLFRIVL 218
+F F F +FY PF F LF ++L
Sbjct: 69 FFFFXFFIFYKKVPFFFFLLFFVLL 143
>CA920109 similar to GP|22535636|dbj hypothetical protein~predicted by
GENSCAN etc. {Oryza sativa (japonica cultivar-group)},
partial (12%)
Length = 625
Score = 28.1 bits (61), Expect = 2.7
Identities = 12/28 (42%), Positives = 16/28 (56%)
Frame = +1
Query: 183 GTSHFWVCLFSYFLFIFILFYISYPFMF 210
G HFW+ L S F F++S PF+F
Sbjct: 532 GRVHFWLILCSII*FSGGFFFLSLPFLF 615
>BQ150627
Length = 411
Score = 28.1 bits (61), Expect = 2.7
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +1
Query: 188 WVCLFSYFLFIFILFYISYPFMFHS 212
+V LFS+ F+ LF S+PF+F S
Sbjct: 325 YVLLFSHIPFLVFLFPFSFPFLFVS 399
>TC84341 homologue to GP|48188|emb|CAA77863.1|| N-terminus of beta subunit
of RNA polymerase {Thermotoga maritima}, partial (3%)
Length = 687
Score = 28.1 bits (61), Expect = 2.7
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -1
Query: 188 WVCLFSYFLFIFILFYISYPFMFHSLF 214
++C F+ F F F Y+ + F+F SLF
Sbjct: 453 FLCHFTNFPFFFYFIYLLFLFLFFSLF 373
>BQ158066 similar to GP|15293967|gb| polyprotein {Potato virus Y}, partial
(5%)
Length = 1076
Score = 28.1 bits (61), Expect = 2.7
Identities = 19/57 (33%), Positives = 24/57 (41%), Gaps = 5/57 (8%)
Frame = -2
Query: 2 TSENNKKP-DHDDELRSNYD----TPKKLSLPTILTRPGFSSEAPTPPPRTTVISIP 53
T NN P D ++ R+ Y TP RP + PTPPPR I+ P
Sbjct: 223 TDPNNGAPNDKQNK*RTGYAKDS*TPNIKKQRGKTDRPAWGHRTPTPPPRPRSIAFP 53
>TC81827 similar to GP|9758799|dbj|BAB09252.1 serine/threonine protein
kinase-like {Arabidopsis thaliana}, partial (14%)
Length = 824
Score = 27.7 bits (60), Expect = 3.6
Identities = 15/33 (45%), Positives = 21/33 (63%), Gaps = 3/33 (9%)
Frame = +2
Query: 189 VCLFSYFLF---IFILFYISYPFMFHSLFRIVL 218
+CLFSYF+F I LF + + M HS F I++
Sbjct: 98 LCLFSYFIFQSWIQFLFCLIFHLMDHS*FIILV 196
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.137 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,344,400
Number of Sequences: 36976
Number of extensions: 144524
Number of successful extensions: 1899
Number of sequences better than 10.0: 77
Number of HSP's better than 10.0 without gapping: 1839
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1891
length of query: 218
length of database: 9,014,727
effective HSP length: 92
effective length of query: 126
effective length of database: 5,612,935
effective search space: 707229810
effective search space used: 707229810
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC149127.3