BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149050.11 - phase: 0
(163 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC92103 101 4e-34
BE997653 similar to GP|22093732|dbj contains EST AU069790(E3307)... 123 3e-29
TC78208 similar to PIR|H96703|H96703 probable RING zinc finger p... 117 2e-27
TC79126 similar to PIR|H96703|H96703 probable RING zinc finger p... 115 6e-27
TC79172 similar to GP|9502381|gb|AAF88088.1| T12C24.29 {Arabidop... 101 1e-22
TC91231 similar to GP|9502381|gb|AAF88088.1| T12C24.29 {Arabidop... 85 1e-17
TC86681 similar to GP|21553392|gb|AAM62485.1 unknown {Arabidopsi... 69 1e-12
BF634100 similar to GP|18377636|gb| unknown protein {Arabidopsis... 66 7e-12
BF645370 weakly similar to GP|12321864|gb| RING zinc finger prot... 65 9e-12
TC80947 weakly similar to GP|9759560|dbj|BAB11162.1 C3HC4-type R... 62 1e-10
TC78411 similar to PIR|T47692|T47692 hypothetical protein T22E16... 60 4e-10
TC85903 similar to PIR|B84530|B84530 probable RING zinc finger p... 57 3e-09
TC77620 similar to GP|10129643|emb|CAC08239. Pspzf zinc finger p... 57 3e-09
TC78547 similar to GP|12003386|gb|AAG43550.1 Avr9/Cf-9 rapidly e... 57 3e-09
TC83859 57 3e-09
BG452562 similar to GP|9293902|dbj contains similarity to RING z... 57 4e-09
TC84560 similar to GP|17978924|gb|AAL47429.1 AT5g45290/K9E15_7 {... 57 4e-09
CA921430 similar to GP|17978924|gb AT5g45290/K9E15_7 {Arabidopsi... 56 6e-09
AJ501352 similar to PIR|B84584|B845 probable RING zinc finger pr... 55 1e-08
AW584677 similar to GP|18491265|gb| At2g46160/T3F17.19 {Arabidop... 54 2e-08
>TC92103
Length = 658
Score = 101 bits (251), Expect(2) = 4e-34
Identities = 50/53 (94%), Positives = 51/53 (95%)
Frame = +1
Query: 9 LTMVCLSLNEFFDAVFSFWIYLIVFGALFCIVQIIALAYALTQPLRIREGASE 61
LTMVCLSLNEFFDAVFSFWIYLIVFGALFCIVQIIALAYALTQPLRIR AS+
Sbjct: 409 LTMVCLSLNEFFDAVFSFWIYLIVFGALFCIVQIIALAYALTQPLRIRXXASK 567
Score = 59.7 bits (143), Expect(2) = 4e-34
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = +2
Query: 63 DIKSLPMYRFCQPNVMIMVDKNKTQLEAR 91
DIKSL MYRFCQPNVMIMVDKNKTQLEAR
Sbjct: 572 DIKSLXMYRFCQPNVMIMVDKNKTQLEAR 658
>BE997653 similar to GP|22093732|dbj contains EST AU069790(E3307)~similar to
Arabidopsis thaliana chromosome1 T12C24.29~unknown
protein, partial (12%)
Length = 454
Score = 123 bits (309), Expect = 3e-29
Identities = 52/53 (98%), Positives = 52/53 (98%)
Frame = +3
Query: 111 ECCICLCPYVDGTELYRLPCTHHFHCECIGRWLRTKATCPLCKFNIRIGDIMV 163
E CICLCPYVDGTELYRLPCTHHFHCECIGRWLRTKATCPLCKFNIRIGDIMV
Sbjct: 3 EGCICLCPYVDGTELYRLPCTHHFHCECIGRWLRTKATCPLCKFNIRIGDIMV 161
>TC78208 similar to PIR|H96703|H96703 probable RING zinc finger protein
T23K23.8 [imported] - Arabidopsis thaliana, partial
(72%)
Length = 1132
Score = 117 bits (293), Expect = 2e-27
Identities = 68/157 (43%), Positives = 90/157 (57%), Gaps = 5/157 (3%)
Frame = +3
Query: 9 LTMVCLSLNEFFDAVFSFWIYLIVFGALFCIVQ-IIALAYALTQPLRIREGASEDDIKSL 67
L +V L+ + FF AVF + ++ AL C + II + YA+ +EGASE D+ +L
Sbjct: 690 LAVVFLAFDVFF-AVFCVALACLIGIALCCCLPCIIGILYAVAG----QEGASESDLSTL 854
Query: 68 PMYRFCQPNVMIMVDKNKTQLEARTGS----HNRSHISELSLHPDDSECCICLCPYVDGT 123
P YRF P N+ + GS N S +E L P+D+ECCIC+ PY D
Sbjct: 855 PKYRFQVPG-------NEETPSPKGGSMVPIENSSGANERVLSPEDAECCICISPYEDEA 1013
Query: 124 ELYRLPCTHHFHCECIGRWLRTKATCPLCKFNIRIGD 160
EL+ LPC HHFH CI +WL+ ATCPLCKFNI G+
Sbjct: 1014ELHALPCNHHFHSTCIVKWLKMNATCPLCKFNIXKGN 1124
>TC79126 similar to PIR|H96703|H96703 probable RING zinc finger protein
T23K23.8 [imported] - Arabidopsis thaliana, partial
(81%)
Length = 1397
Score = 115 bits (289), Expect = 6e-27
Identities = 69/162 (42%), Positives = 92/162 (56%), Gaps = 7/162 (4%)
Frame = +3
Query: 9 LTMVCLSLNEFFDAVFSFWIYLIVFGALFCIVQ-IIALAYALTQPLRIREGASEDDIKSL 67
L +V L+ + FF A+F + ++ AL C + IIA+ YA+ +EGASE D+ L
Sbjct: 810 LAVVFLAFDVFF-AIFCVVLACLIGIALCCCLPCIIAILYAVAG----QEGASEADLSIL 974
Query: 68 PMYRFCQPNVMIMVDKNKTQLEARTGSH------NRSHISELSLHPDDSECCICLCPYVD 121
P YRF N N+ + GS + +E L P+D+ECCICLCPY D
Sbjct: 975 PKYRFRLSN-------NEEKDSGGAGSMVPIETTSAYLATERILLPEDAECCICLCPYED 1133
Query: 122 GTELYRLPCTHHFHCECIGRWLRTKATCPLCKFNIRIGDIMV 163
G EL+ LPC HHFH CI +WL+ ATCPLCK+NI G+ V
Sbjct: 1134GVELHTLPCNHHFHSSCIVKWLKMNATCPLCKYNILKGNEQV 1259
>TC79172 similar to GP|9502381|gb|AAF88088.1| T12C24.29 {Arabidopsis
thaliana}, partial (71%)
Length = 1589
Score = 101 bits (252), Expect = 1e-22
Identities = 59/155 (38%), Positives = 79/155 (50%), Gaps = 7/155 (4%)
Frame = +2
Query: 9 LTMVCLSLNEFFDAVFSFWIYLIVFGALFCIVQIIALAYALTQPLRIREGASEDDIKSLP 68
L +V L + FF +I C+ IIAL YA+ +EGAS++DI+ L
Sbjct: 887 LCIVFLGFDVFFVVFCVALACVIGIAVCCCLPCIIALLYAVAD----QEGASKEDIEQLS 1054
Query: 69 MYRFCQPNVMIMVDKNKTQLEARTG-------SHNRSHISELSLHPDDSECCICLCPYVD 121
++F + V+ N+ Q + G E L +D+ECCICL Y D
Sbjct: 1055 KFKFRK------VESNEKQTDNNQGPVGGIMTECRADSPIEHVLAEEDAECCICLSSYDD 1216
Query: 122 GTELYRLPCTHHFHCECIGRWLRTKATCPLCKFNI 156
G EL LPC HHFHC C+ +WL ATCPLCK+NI
Sbjct: 1217 GVELRELPCGHHFHCACVDKWLYINATCPLCKYNI 1321
>TC91231 similar to GP|9502381|gb|AAF88088.1| T12C24.29 {Arabidopsis
thaliana}, partial (17%)
Length = 728
Score = 84.7 bits (208), Expect = 1e-17
Identities = 32/52 (61%), Positives = 39/52 (74%)
Frame = +1
Query: 105 LHPDDSECCICLCPYVDGTELYRLPCTHHFHCECIGRWLRTKATCPLCKFNI 156
L +D+ECCICL Y DG EL +LPC HHFHC C+ +WL ATCPLCK+N+
Sbjct: 73 LADEDAECCICLSAYDDGAELRQLPCGHHFHCTCVDKWLHINATCPLCKYNM 228
>TC86681 similar to GP|21553392|gb|AAM62485.1 unknown {Arabidopsis
thaliana}, partial (14%)
Length = 607
Score = 68.6 bits (166), Expect = 1e-12
Identities = 30/58 (51%), Positives = 38/58 (64%)
Frame = +2
Query: 96 NRSHISELSLHPDDSECCICLCPYVDGTELYRLPCTHHFHCECIGRWLRTKATCPLCK 153
N S SE L D+ ECCICL Y D E+ +LPC+H FH +C+ +WLR + CPLCK
Sbjct: 74 NDSQCSE-RLXXDEPECCICLAKYKDKEEVRQLPCSHMFHLKCVDQWLRIISCCPLCK 244
>BF634100 similar to GP|18377636|gb| unknown protein {Arabidopsis thaliana},
partial (14%)
Length = 376
Score = 65.9 bits (159), Expect = 7e-12
Identities = 24/49 (48%), Positives = 33/49 (66%)
Frame = +2
Query: 108 DDSECCICLCPYVDGTELYRLPCTHHFHCECIGRWLRTKATCPLCKFNI 156
+D+ CCICL Y + EL LPC+H FH +C+ +WL+ A CPLCK +
Sbjct: 71 EDAVCCICLAKYENNDELRELPCSHLFHKDCVDKWLKINALCPLCKSEV 217
>BF645370 weakly similar to GP|12321864|gb| RING zinc finger protein;
17145-17621 {Arabidopsis thaliana}, partial (48%)
Length = 654
Score = 65.5 bits (158), Expect = 9e-12
Identities = 23/52 (44%), Positives = 40/52 (76%), Gaps = 1/52 (1%)
Frame = +1
Query: 108 DDSECCICLCPYVDGTELYRLP-CTHHFHCECIGRWLRTKATCPLCKFNIRI 158
+++ECCICL + DG +L LP C H+FHC+C+ +WL +++CPLC+ ++++
Sbjct: 397 EETECCICLGEFRDGEKLKVLPGCEHYFHCDCVDKWLTHQSSCPLCRGSLKV 552
>TC80947 weakly similar to GP|9759560|dbj|BAB11162.1 C3HC4-type RING zinc
finger protein-like {Arabidopsis thaliana}, partial (46%)
Length = 1445
Score = 62.0 bits (149), Expect = 1e-10
Identities = 25/48 (52%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
Frame = +2
Query: 107 PDDSECCICLCPYVDGTELYRLP-CTHHFHCECIGRWLRTKATCPLCK 153
P D+ C ICLC Y L +P C H+FH CI WL+T ATCPLC+
Sbjct: 1145 PSDNTCSICLCEYQPNEVLRTIPECNHYFHVNCIDGWLKTNATCPLCR 1288
>TC78411 similar to PIR|T47692|T47692 hypothetical protein T22E16.190 -
Arabidopsis thaliana, partial (81%)
Length = 1378
Score = 60.1 bits (144), Expect = 4e-10
Identities = 32/107 (29%), Positives = 50/107 (45%), Gaps = 7/107 (6%)
Frame = +2
Query: 60 SEDDIKSLPMYRFCQPNVMIMVDKNKTQLEARTGS-------HNRSHISELSLHPDDSEC 112
+E++I +LP++++ K+ + ++ T S N + + D+ C
Sbjct: 713 TEEEINALPVHKYKVSGPQ----KSSSSMQQATPSTSAEKTQDNLNAVGNSKASDDELTC 880
Query: 113 CICLCPYVDGTELYRLPCTHHFHCECIGRWLRTKATCPLCKFNIRIG 159
+CL G L LPC H FH CI WLR + TCP+CKF G
Sbjct: 881 SVCLEQVNVGDVLRSLPCLHQFHASCIDPWLRQQGTCPVCKFRAGSG 1021
>TC85903 similar to PIR|B84530|B84530 probable RING zinc finger protein
[imported] - Arabidopsis thaliana, partial (26%)
Length = 2572
Score = 57.4 bits (137), Expect = 3e-09
Identities = 27/58 (46%), Positives = 33/58 (56%), Gaps = 1/58 (1%)
Frame = +3
Query: 97 RSHISELSLHPDDSE-CCICLCPYVDGTELYRLPCTHHFHCECIGRWLRTKATCPLCK 153
R ++S ++ D E CCIC YVDG L L C H FH CI +WL K CP+CK
Sbjct: 2094 RFYMSLMTESSSDLEPCCICQEEYVDGQNLGLLDCGHEFHSNCITQWLMQKNLCPICK 2267
>TC77620 similar to GP|10129643|emb|CAC08239. Pspzf zinc finger protein-like
{Arabidopsis thaliana}, partial (26%)
Length = 1958
Score = 57.4 bits (137), Expect = 3e-09
Identities = 23/49 (46%), Positives = 31/49 (62%)
Frame = +3
Query: 108 DDSECCICLCPYVDGTELYRLPCTHHFHCECIGRWLRTKATCPLCKFNI 156
DD +CCIC YV+ E+ L C H +H CI +WLR K CP+CK ++
Sbjct: 1602 DDIKCCICQEEYVEEDEVGSLLCEHKYHVVCIQQWLRLKNWCPICKASV 1748
>TC78547 similar to GP|12003386|gb|AAG43550.1 Avr9/Cf-9 rapidly elicited
protein 132 {Nicotiana tabacum}, partial (41%)
Length = 1648
Score = 57.0 bits (136), Expect = 3e-09
Identities = 22/47 (46%), Positives = 30/47 (63%), Gaps = 1/47 (2%)
Frame = +2
Query: 108 DDSECCICLCPYVDGTELYRLP-CTHHFHCECIGRWLRTKATCPLCK 153
D EC +CLC V+G + LP C H FH +CI W ++ +TCPLC+
Sbjct: 614 DGLECAVCLCDVVEGEKTRLLPKCNHGFHLDCIDMWFQSHSTCPLCR 754
>TC83859
Length = 554
Score = 57.0 bits (136), Expect = 3e-09
Identities = 35/136 (25%), Positives = 60/136 (43%), Gaps = 1/136 (0%)
Frame = +3
Query: 23 VFSFWIYLIVFGALFCIVQIIALAYALTQPLRIREGASEDDIKSLPMYRFCQPNVMIMVD 82
+F +W++L + I+ + + Y + + D + P Y+ N I+ +
Sbjct: 21 IFGYWLFLYQNMTITMIMNYLTICYTHLKWVL-------DILTYYPFYKLHDSNFPIIGE 179
Query: 83 KNKTQLEARTGSHNRSHISELSLHPDDSECCICLCPYVDGTELYRLPCTHHFHCECIGRW 142
+N H +E D EC +CLC +G E+ L C H +H C+ +W
Sbjct: 180 MYDI--------YNYEHTNE------DVECVVCLCKIEEGDEISVLRCDHMYHKHCLDKW 317
Query: 143 LRTK-ATCPLCKFNIR 157
L K TCPLC+ ++R
Sbjct: 318 LSFKNHTCPLCRESLR 365
>BG452562 similar to GP|9293902|dbj contains similarity to RING zinc finger
protein~gene_id:MVE11.15 {Arabidopsis thaliana}, partial
(59%)
Length = 664
Score = 56.6 bits (135), Expect = 4e-09
Identities = 24/57 (42%), Positives = 36/57 (63%), Gaps = 1/57 (1%)
Frame = +2
Query: 98 SHISELSLHPDDSECCICLCPYVDGTELYRLP-CTHHFHCECIGRWLRTKATCPLCK 153
S+ +EL L D+EC ICL + G ++ LP C H FH CI +WL++ ++CP C+
Sbjct: 404 SYSTELKLPTLDTECVICLSEFTKGEKVRILPKCNHGFHVRCIDKWLKSHSSCPKCR 574
>TC84560 similar to GP|17978924|gb|AAL47429.1 AT5g45290/K9E15_7 {Arabidopsis
thaliana}, partial (12%)
Length = 1044
Score = 56.6 bits (135), Expect = 4e-09
Identities = 21/50 (42%), Positives = 30/50 (60%), Gaps = 1/50 (2%)
Frame = +1
Query: 108 DDSECCICLCPYVDGTELYRLPCTHHFHCECIGRWLR-TKATCPLCKFNI 156
D +C ICL Y DG ++ LPC H +H C+ +WL+ CPLC+ N+
Sbjct: 499 DAEQCYICLAEYEDGDQIRVLPCKHEYHMSCVDKWLKEIHGVCPLCRSNV 648
>CA921430 similar to GP|17978924|gb AT5g45290/K9E15_7 {Arabidopsis thaliana},
partial (20%)
Length = 585
Score = 56.2 bits (134), Expect = 6e-09
Identities = 26/62 (41%), Positives = 36/62 (57%), Gaps = 2/62 (3%)
Frame = -3
Query: 101 SELSLHPDDS-ECCICLCPYVDGTELYRLPCTHHFHCECIGRWLR-TKATCPLCKFNIRI 158
++L H ++ +C ICL Y DG + LPC H FH CI +WL+ CPLC+ +I I
Sbjct: 304 TKLQKHQEEPVQCYICLVEYEDGDSMRVLPCHHEFHTTCIDKWLKEVHRVCPLCRGDICI 125
Query: 159 GD 160
D
Sbjct: 124 SD 119
>AJ501352 similar to PIR|B84584|B845 probable RING zinc finger protein
[imported] - Arabidopsis thaliana, partial (17%)
Length = 607
Score = 55.1 bits (131), Expect = 1e-08
Identities = 23/52 (44%), Positives = 31/52 (59%), Gaps = 1/52 (1%)
Frame = +3
Query: 111 ECCICLCPYVDGTELYRLP-CTHHFHCECIGRWLRTKATCPLCKFNIRIGDI 161
EC +CL + D L LP C H FH CI +WL + +TCPLC++ + DI
Sbjct: 408 ECTVCLSKFEDEEILRLLPKCKHAFHMNCIDKWLESHSTCPLCRYMVEESDI 563
>AW584677 similar to GP|18491265|gb| At2g46160/T3F17.19 {Arabidopsis
thaliana}, partial (34%)
Length = 611
Score = 54.3 bits (129), Expect = 2e-08
Identities = 21/46 (45%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
Frame = +2
Query: 109 DSECCICLCPYVDGTELYRLP-CTHHFHCECIGRWLRTKATCPLCK 153
DS C ICLC Y D L +P C H+FH C+ WL+ +CP+C+
Sbjct: 173 DSTCSICLCEYKDSEMLRMMPECRHYFHLCCLDSWLKLNGSCPVCR 310
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.332 0.143 0.482
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,370,290
Number of Sequences: 36976
Number of extensions: 127567
Number of successful extensions: 1387
Number of sequences better than 10.0: 189
Number of HSP's better than 10.0 without gapping: 1346
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1355
length of query: 163
length of database: 9,014,727
effective HSP length: 89
effective length of query: 74
effective length of database: 5,723,863
effective search space: 423565862
effective search space used: 423565862
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 54 (25.4 bits)
Medicago: description of AC149050.11