BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148996.5 + phase: 0
(196 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80580 similar to GP|9294319|dbj|BAB02216.1 contains similarity... 354 1e-98
TC79939 similar to GP|9758423|dbj|BAB08965.1 gene_id:MHF15.5~sim... 33 0.071
TC92965 weakly similar to GP|10764862|gb|AAF24552.2 F1K23.23 {Ar... 33 0.093
TC81248 similar to SP|O81004|YB87_ARATH Hypothetical GTP-binding... 32 0.12
BF647147 weakly similar to GP|7303636|gb|A CG13214-PA {Drosophil... 32 0.21
TC77956 similar to GP|16323053|gb|AAL15261.1 AT3g01640/F4P13_18 ... 32 0.21
TC89029 weakly similar to GP|15450467|gb|AAK96527.1 At1g12810/F1... 32 0.21
AW697263 weakly similar to GP|9759418|dbj| dbj|BAA84609.1~gene_i... 30 0.46
BI272394 30 0.46
TC88766 similar to PIR|T04745|T04745 hypothetical protein F6G17.... 30 0.60
TC80081 weakly similar to PIR|D96525|D96525 protein T1N15.19 [im... 30 0.60
TC83133 similar to PIR|T06033|T06033 hypothetical protein T28I19... 30 0.60
TC93142 similar to GP|22535672|dbj|BAC10846. metal-transporting ... 29 1.0
TC93414 homologue to GP|17907114|emb|CAD13249. tyrosine kinase {... 29 1.3
TC89702 similar to PIR|T01157|T01157 hypothetical protein F7N22.... 28 1.7
TC81406 weakly similar to PIR|T08954|T08954 hypothetical protein... 28 1.7
AW685354 weakly similar to GP|6714472|gb| hypothetical protein {... 28 1.7
TC82728 similar to GP|7292941|gb|AAF48332.1| CG11071-PA {Drosoph... 28 2.3
TC90459 similar to GP|9758598|dbj|BAB09231.1 gene_id:MDF20.8~unk... 28 2.3
TC87418 similar to PIR|T51264|T51264 calcium-dependent protein k... 28 3.0
>TC80580 similar to GP|9294319|dbj|BAB02216.1 contains similarity to unknown
protein~emb|CAB43041.1~gene_id:K24M9.7 {Arabidopsis
thaliana}, partial (61%)
Length = 917
Score = 354 bits (908), Expect = 1e-98
Identities = 178/191 (93%), Positives = 180/191 (94%), Gaps = 1/191 (0%)
Frame = +1
Query: 1 MAKFSISRTSTLYRSLLSTPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTE 60
MAKFSISRTSTLYRSLLSTPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTE
Sbjct: 37 MAKFSISRTSTLYRSLLSTPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTE 216
Query: 61 RTFFDRPLENGLDPGVYRAILVGKAGQKPLQKKLKSGTVVTLLSIGTGGIRNNRRPLDHE 120
RTFFDRPLENGLDPGVYRAILVGKAGQKPLQKKLKSGTVVTLLSIGTGGIRNNRRPLDHE
Sbjct: 217 RTFFDRPLENGLDPGVYRAILVGKAGQKPLQKKLKSGTVVTLLSIGTGGIRNNRRPLDHE 396
Query: 121 NPREYANRCAVQWHRVTVYPERLGN-LLMKTVLPGSTLYVEGNLETKVFSDPVTGLVRRV 179
NPREYANRCAVQ HR +G LLMKTVLPGSTLYVEGNLETKVFSDPVTGLVRRV
Sbjct: 397 NPREYANRCAVQCHRGYCLSREIGXILLMKTVLPGSTLYVEGNLETKVFSDPVTGLVRRV 576
Query: 180 REVAVRRNGNL 190
REVAVRRNG +
Sbjct: 577 REVAVRRNGRV 609
>TC79939 similar to GP|9758423|dbj|BAB08965.1 gene_id:MHF15.5~similar to
unknown protein~sp|P73920 {Arabidopsis thaliana},
partial (57%)
Length = 981
Score = 33.1 bits (74), Expect = 0.071
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = -2
Query: 17 LSTPPPPLHHHRHIPLRFCTTTTSLSD 43
L+ PPPP HHR + L C+ + S+SD
Sbjct: 326 LAAPPPPAWHHRKLFLPSCSMSNSISD 246
>TC92965 weakly similar to GP|10764862|gb|AAF24552.2 F1K23.23 {Arabidopsis
thaliana}, partial (25%)
Length = 697
Score = 32.7 bits (73), Expect = 0.093
Identities = 16/27 (59%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = +3
Query: 15 SLLSTPPPPLHHHRHI-PLRFCTTTTS 40
SLL TPPPP HH H+ PL F T S
Sbjct: 108 SLLPTPPPPQHHPLHLHPLLFTTKDNS 188
>TC81248 similar to SP|O81004|YB87_ARATH Hypothetical GTP-binding protein
At2g22870. [Mouse-ear cress] {Arabidopsis thaliana},
partial (78%)
Length = 1177
Score = 32.3 bits (72), Expect = 0.12
Identities = 23/71 (32%), Positives = 36/71 (50%), Gaps = 1/71 (1%)
Frame = +2
Query: 5 SISRTSTLYRSLLSTPPPPLHHHRHIPLRFCTTTTS-LSDYDDADSAAPSPSPEQTERTF 63
++ R S L TPP PL H +P+ TTT S LS + + A+ + +P +E+ F
Sbjct: 53 ALRRASITLTFLFQTPPSPLTHTFSLPIPKRTTTPSFLSLHHSSIPASKTLTPPPSEKPF 232
Query: 64 FDRPLENGLDP 74
P G++P
Sbjct: 233 LFIP--PGVEP 259
>BF647147 weakly similar to GP|7303636|gb|A CG13214-PA {Drosophila
melanogaster}, partial (8%)
Length = 426
Score = 31.6 bits (70), Expect = 0.21
Identities = 16/39 (41%), Positives = 17/39 (43%)
Frame = +3
Query: 20 PPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQ 58
PPPP HHH H P S Y D P P+P Q
Sbjct: 147 PPPPHHHHHHGP-----PHDSYQGYFDNGYPPPPPAPPQ 248
>TC77956 similar to GP|16323053|gb|AAL15261.1 AT3g01640/F4P13_18
{Arabidopsis thaliana}, partial (90%)
Length = 1412
Score = 31.6 bits (70), Expect = 0.21
Identities = 15/25 (60%), Positives = 16/25 (64%)
Frame = -2
Query: 18 STPPPPLHHHRHIPLRFCTTTTSLS 42
S PPPPLH H H L F T+ SLS
Sbjct: 112 SPPPPPLHDHHHHLLPF*FTSFSLS 38
>TC89029 weakly similar to GP|15450467|gb|AAK96527.1 At1g12810/F13K23_4
{Arabidopsis thaliana}, partial (71%)
Length = 643
Score = 31.6 bits (70), Expect = 0.21
Identities = 16/39 (41%), Positives = 17/39 (43%)
Frame = +3
Query: 20 PPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQ 58
PPPP HHH H P S Y D P P+P Q
Sbjct: 174 PPPPHHHHHHGP-----PHDSYQGYFDNGYPPPPPAPPQ 275
>AW697263 weakly similar to GP|9759418|dbj|
dbj|BAA84609.1~gene_id:K7J8.2~similar to unknown protein
{Arabidopsis thaliana}, partial (7%)
Length = 829
Score = 30.4 bits (67), Expect = 0.46
Identities = 13/20 (65%), Positives = 13/20 (65%)
Frame = +2
Query: 10 STLYRSLLSTPPPPLHHHRH 29
S RSLLS P PP HHRH
Sbjct: 122 SLFLRSLLSPPAPPCLHHRH 181
>BI272394
Length = 199
Score = 30.4 bits (67), Expect = 0.46
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 5/38 (13%)
Frame = +2
Query: 11 TLYRSLLSTPPPP--LHHHR---HIPLRFCTTTTSLSD 43
+L+ S+PPPP LHHH+ H L +C +T L D
Sbjct: 17 SLFTLKFSSPPPPXFLHHHQAAPHRTLPYCDSTFQLLD 130
>TC88766 similar to PIR|T04745|T04745 hypothetical protein F6G17.160 -
Arabidopsis thaliana, partial (34%)
Length = 813
Score = 30.0 bits (66), Expect = 0.60
Identities = 14/67 (20%), Positives = 33/67 (48%), Gaps = 1/67 (1%)
Frame = +3
Query: 4 FSISRTSTLYRSL-LSTPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTERT 62
+++ + +++S+ L PP+ H+ H P +F T+ + + S +P P+ +
Sbjct: 18 YNVKTNTKIHKSIPLIFTNPPIFHYLHFPSQFNPLQTTTMELSSSFSLSPKPTNFSFSSS 197
Query: 63 FFDRPLE 69
F P++
Sbjct: 198FSPFPIQ 218
>TC80081 weakly similar to PIR|D96525|D96525 protein T1N15.19 [imported] -
Arabidopsis thaliana, partial (30%)
Length = 1098
Score = 30.0 bits (66), Expect = 0.60
Identities = 16/45 (35%), Positives = 25/45 (55%)
Frame = +1
Query: 22 PPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTERTFFDR 66
PPLHHHRH +T++ + +++ S+ P PE +F DR
Sbjct: 211 PPLHHHRHQTSFSAFSTSTAAAEEESLSSTVHPWPEWI--SFVDR 339
>TC83133 similar to PIR|T06033|T06033 hypothetical protein T28I19.140 -
Arabidopsis thaliana, partial (16%)
Length = 906
Score = 30.0 bits (66), Expect = 0.60
Identities = 20/59 (33%), Positives = 27/59 (44%), Gaps = 5/59 (8%)
Frame = +1
Query: 129 CAVQWHRVTVYPERLGNLLMKTVLPGSTLYVEGNLETKVFS-----DPVTGLVRRVREV 182
CAV W + + ER+ LL L GNL ++ + DP+T V RVR V
Sbjct: 706 CAVLWDKQLKFQERVNRLLAMVASSFVVLVRTGNLLLELVT*LLNHDPITS*VLRVRPV 882
>TC93142 similar to GP|22535672|dbj|BAC10846. metal-transporting P-type
ATPase-like protein {Oryza sativa (japonica
cultivar-group)}, partial (32%)
Length = 689
Score = 29.3 bits (64), Expect = 1.0
Identities = 15/28 (53%), Positives = 20/28 (70%), Gaps = 4/28 (14%)
Frame = +1
Query: 25 HHHRHIPLRFCT-TTTSLSDY---DDAD 48
HHHR +PLR C+ +TTS D+ +DAD
Sbjct: 202 HHHRVLPLRCCSASTTSSGDHLHNNDAD 285
>TC93414 homologue to GP|17907114|emb|CAD13249. tyrosine kinase {Schistosoma
mansoni}, partial (1%)
Length = 578
Score = 28.9 bits (63), Expect = 1.3
Identities = 13/28 (46%), Positives = 14/28 (49%)
Frame = +1
Query: 13 YRSLLSTPPPPLHHHRHIPLRFCTTTTS 40
Y PP HHH+H L TTTTS
Sbjct: 142 YHKTFMVEAPPQHHHQHHQLPPPTTTTS 225
>TC89702 similar to PIR|T01157|T01157 hypothetical protein F7N22.3 -
Arabidopsis thaliana, partial (67%)
Length = 988
Score = 28.5 bits (62), Expect = 1.7
Identities = 22/60 (36%), Positives = 30/60 (49%), Gaps = 9/60 (15%)
Frame = +3
Query: 15 SLLSTPPP--PLHHHRHIP------LRFCTTTTSLSDYDDADSAAPS-PSPEQTERTFFD 65
S+ PPP LHH P L +TTT+L+ + SA+P P+P T+R F D
Sbjct: 3 SITQQPPPLPSLHHPILKPTITRRSLLLTSTTTTLTFPSLSSSASPQLPNPTITDRIFMD 182
Score = 26.2 bits (56), Expect = 8.7
Identities = 13/38 (34%), Positives = 19/38 (49%)
Frame = +1
Query: 19 TPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSP 56
+PPPP H ++ P ++ TS S APS +P
Sbjct: 121 SPPPPHHSYQTPPSPTASSWTSASAPTITSQIAPSATP 234
>TC81406 weakly similar to PIR|T08954|T08954 hypothetical protein
F19B15.20 - Arabidopsis thaliana, partial (20%)
Length = 747
Score = 28.5 bits (62), Expect = 1.7
Identities = 20/56 (35%), Positives = 26/56 (45%), Gaps = 4/56 (7%)
Frame = +3
Query: 9 TSTLYRSLLSTPP---PPLHHHRHIP-LRFCTTTTSLSDYDDADSAAPSPSPEQTE 60
T+ L+ + ST P PP HHH H P L SL++ +AA S E E
Sbjct: 15 TNLLFPIMSSTSPSQSPPSHHHHHQPLLSSLVVRPSLTENSPPGAAAHSNDYEPGE 182
>AW685354 weakly similar to GP|6714472|gb| hypothetical protein {Arabidopsis
thaliana}, partial (19%)
Length = 618
Score = 28.5 bits (62), Expect = 1.7
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -1
Query: 6 ISRTSTLYRSLLSTPPPPLHHHRHIPLRFC 35
IS T+ RS PP P HHH H+ + +C
Sbjct: 585 ISCTTXYXRS----PPYPWHHHLHLHVNYC 508
>TC82728 similar to GP|7292941|gb|AAF48332.1| CG11071-PA {Drosophila
melanogaster}, partial (2%)
Length = 734
Score = 28.1 bits (61), Expect = 2.3
Identities = 13/30 (43%), Positives = 16/30 (53%), Gaps = 1/30 (3%)
Frame = -2
Query: 16 LLSTPPPPLHHHRHIPLRFCTT-TTSLSDY 44
L+S P P HHH H R T +LS+Y
Sbjct: 466 LISDPKSPFHHHHHYHRRHTNAKTKALSEY 377
>TC90459 similar to GP|9758598|dbj|BAB09231.1 gene_id:MDF20.8~unknown
protein {Arabidopsis thaliana}, partial (58%)
Length = 953
Score = 28.1 bits (61), Expect = 2.3
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +2
Query: 24 LHHHRHIPLRFCTTTTSLS 42
+HH RH RFCT T SLS
Sbjct: 695 MHHSRHGLQRFCTLTISLS 751
>TC87418 similar to PIR|T51264|T51264 calcium-dependent protein kinase-like
- Arabidopsis thaliana, partial (30%)
Length = 1206
Score = 27.7 bits (60), Expect = 3.0
Identities = 27/102 (26%), Positives = 39/102 (37%), Gaps = 11/102 (10%)
Frame = +1
Query: 8 RTSTLYRSLLSTPPPPL-HHHRHIPLRFC--------TTTTSLSDYDDADSAAPSPS-PE 57
R ++ +L PPPP HH H P + + S A+S S S P
Sbjct: 502 RENSTNEEILQLPPPPSSSHHHHAPTKSSSKFPFFSPSPLPSFFKNSPANSTVSSNSTPL 681
Query: 58 QTERTFFDRPLENGLDPGVYRAILVGKAGQ-KPLQKKLKSGT 98
+T F RP RA+L + G KP + + G+
Sbjct: 682 RTPLRIFKRPFPPPSPAKHIRALLARRHGSVKPNEATIPEGS 807
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.134 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,398,004
Number of Sequences: 36976
Number of extensions: 104399
Number of successful extensions: 1403
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 1277
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1361
length of query: 196
length of database: 9,014,727
effective HSP length: 91
effective length of query: 105
effective length of database: 5,649,911
effective search space: 593240655
effective search space used: 593240655
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC148996.5