BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148996.16 - phase: 0 /pseudo
(332 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90372 similar to GP|20197166|gb|AAC27402.2 expressed protein {... 243 1e-88
BG585783 similar to GP|20197166|gb expressed protein {Arabidopsi... 249 2e-86
TC78188 similar to PIR|C84870|C84870 probable splicing factor [i... 43 2e-04
TC90247 weakly similar to GP|21592898|gb|AAM64848.1 unknown {Ara... 32 0.26
TC78665 homologue to PIR|G96563|G96563 probable coatomer complex... 32 0.26
TC79322 similar to GP|13605815|gb|AAK32893.1 At2g32700/F24L7.16 ... 32 0.44
TC87605 homologue to PIR|G96563|G96563 probable coatomer complex... 32 0.44
TC79260 weakly similar to GP|9759350|dbj|BAB10005.1 WD-repeat pr... 31 0.76
TC78591 similar to GP|9759350|dbj|BAB10005.1 WD-repeat protein-l... 31 0.76
TC82717 weakly similar to GP|21539583|gb|AAM53344.1 putative coa... 30 1.3
BG647724 similar to GP|11141605|gb LEUNIG {Arabidopsis thaliana}... 29 2.2
TC92332 similar to GP|18377648|gb|AAL66974.1 unknown protein {Ar... 28 3.8
TC88481 similar to PIR|T00806|T02445 probable U4/U6 small nuclea... 28 3.8
TC90611 similar to GP|10177223|dbj|BAB10298. contains similarity... 28 3.8
TC80769 similar to GP|11141605|gb|AAG32022.1 LEUNIG {Arabidopsis... 28 4.9
TC78145 similar to GP|15294240|gb|AAK95297.1 AT3g18860/MCB22_3 {... 28 6.4
CB893690 weakly similar to GP|9759025|dbj gene_id:K6A12.9~simila... 28 6.4
TC93456 similar to GP|12711287|emb|CAC28528. GATA-1 zinc finger ... 28 6.4
TC91549 similar to GP|17063189|gb|AAL32989.1 unknown protein {Ar... 27 8.4
>TC90372 similar to GP|20197166|gb|AAC27402.2 expressed protein {Arabidopsis
thaliana}, partial (52%)
Length = 706
Score = 243 bits (620), Expect(2) = 1e-88
Identities = 121/121 (100%), Positives = 121/121 (100%)
Frame = +1
Query: 1 MEINLGKLAFDIDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAA 60
MEINLGKLAFDIDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAA
Sbjct: 88 MEINLGKLAFDIDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAA 267
Query: 61 RFINGGRAVLTGSPDFSILATDVETGSTIARLDNAHEAAVNRLINLTESTVASGDDDGCI 120
RFINGGRAVLTGSPDFSILATDVETGSTIARLDNAHEAAVNRLINLTESTVASGDDDGCI
Sbjct: 268 RFINGGRAVLTGSPDFSILATDVETGSTIARLDNAHEAAVNRLINLTESTVASGDDDGCI 447
Query: 121 K 121
K
Sbjct: 448 K 450
Score = 101 bits (252), Expect(2) = 1e-88
Identities = 48/49 (97%), Positives = 49/49 (99%)
Frame = +2
Query: 121 KTLLLHLTQ*KYWPQVETGLFLFAIFDEIKSKPNLNFLKMSCCLWF**R 169
+TLLLHLTQ*KYWPQVETGLFLFAIFDEIKSKPNLNFLKMSCCLWF**R
Sbjct: 512 QTLLLHLTQ*KYWPQVETGLFLFAIFDEIKSKPNLNFLKMSCCLWF**R 658
>BG585783 similar to GP|20197166|gb expressed protein {Arabidopsis thaliana},
partial (32%)
Length = 743
Score = 249 bits (636), Expect(2) = 2e-86
Identities = 122/122 (100%), Positives = 122/122 (100%)
Frame = +3
Query: 211 RIITGSENGMINLVGILPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLD 270
RIITGSENGMINLVGILPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLD
Sbjct: 177 RIITGSENGMINLVGILPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLD 356
Query: 271 NILQGSRSTQRNETGVVANDGDSDDGDEMDVDNSASKFSKGNKRKNASNGHAVGDSNNFF 330
NILQGSRSTQRNETGVVANDGDSDDGDEMDVDNSASKFSKGNKRKNASNGHAVGDSNNFF
Sbjct: 357 NILQGSRSTQRNETGVVANDGDSDDGDEMDVDNSASKFSKGNKRKNASNGHAVGDSNNFF 536
Query: 331 AD 332
AD
Sbjct: 537 AD 542
Score = 87.8 bits (216), Expect(2) = 2e-86
Identities = 42/45 (93%), Positives = 44/45 (97%)
Frame = +1
Query: 169 RMAGKLFADHKLESCYCIHGDASRIAVIDSLILLQTLLIPC*RII 213
RMAGKLFADHKLESCYCIHGDASRIAVIDSLILLQTLLIPC* ++
Sbjct: 37 RMAGKLFADHKLESCYCIHGDASRIAVIDSLILLQTLLIPC*SLM 171
>TC78188 similar to PIR|C84870|C84870 probable splicing factor [imported] -
Arabidopsis thaliana, partial (95%)
Length = 1436
Score = 42.7 bits (99), Expect = 2e-04
Identities = 30/114 (26%), Positives = 53/114 (46%), Gaps = 2/114 (1%)
Frame = +1
Query: 10 FDIDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAARFINGGRAV 69
+ + F+P+ ++VASG D ++ L+ N + D + + H + + + G +
Sbjct: 325 YTMKFNPTGSVVASGSHDKEIFLW-----NVHGDCKNFMVLKGHKNAVLDLHWTSDGTQI 489
Query: 70 LTGSPDFSILATDVETGSTIARLDNAHEAAVNRLINLTES--TVASGDDDGCIK 121
++ SPD ++ D ETG I ++ H + VN V SG DDG K
Sbjct: 490 ISASPDKTLRLWDTETGKQIKKMVE-HLSYVNSCCPTRRGPPLVVSGSDDGTAK 648
>TC90247 weakly similar to GP|21592898|gb|AAM64848.1 unknown {Arabidopsis
thaliana}, partial (60%)
Length = 1041
Score = 32.3 bits (72), Expect = 0.26
Identities = 52/239 (21%), Positives = 94/239 (38%), Gaps = 36/239 (15%)
Frame = +1
Query: 66 GRAVLTGSPDFSILATDVETGSTIARLDNAHEAAVNRLI-NLTESTVASGDDDGCIKTLL 124
G ++ G+ I +VETG + + +AH AV L+ + +S + SG +DG ++
Sbjct: 352 GTYLVGGALSGDIYLWEVETGRLLKKW-HAHLRAVTCLVFSDDDSLLISGSEDGYVRV-- 522
Query: 125 LHLTQ*KYWPQVETGLFLFAIFDEIKSKPNLNFLKMSCCLWF**RMAGKLFADHKLESCY 184
W LF++FD+++S+ N + S F++H +
Sbjct: 523 --------WS-------LFSLFDDLRSREEKNLYEYS-------------FSEHTMRVTD 618
Query: 185 CIHGDASRIAVIDSL--------------ILLQTLLIPC-----------*RIITGSENG 219
+ G+ A+I S LL++++ P GS +G
Sbjct: 619 VVIGNGGCNAIIVSASDDRTCKVWSLSRGTLLRSIVFPSVINAIVLDPAEHVFYAGSSDG 798
Query: 220 MINLVGILPNR----------IIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWD 268
I + + R II + HS+ V LA+S R L S D ++++W+
Sbjct: 799 KIFIAALNTERITTIDNYGMHIIGSFSNHSK-AVTCLAYSKGRNLLISGSEDGIVRVWN 972
>TC78665 homologue to PIR|G96563|G96563 probable coatomer complex subunit
33791-27676 [imported] - Arabidopsis thaliana, partial
(21%)
Length = 715
Score = 32.3 bits (72), Expect = 0.26
Identities = 29/111 (26%), Positives = 51/111 (45%), Gaps = 1/111 (0%)
Frame = +2
Query: 12 IDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAARFINGGRAVLT 71
+D HP+E + + L G + ++ Y S T + V E+ R+A+FI + V+
Sbjct: 185 VDLHPTEPWILASLYSGTVCIWNYQS-QTMAKSFEVTEL-----PVRSAKFIARKQWVVA 346
Query: 72 GSPDFSILATDVETGSTIARLDNAHEAAVNRL-INLTESTVASGDDDGCIK 121
G+ D I + T + ++ AH + + ++ T V S DD IK
Sbjct: 347 GADDMFIRVYNYNTMDKV-KVFEAHSDYIRCVAVHPTLPYVLSSSDDMLIK 496
>TC79322 similar to GP|13605815|gb|AAK32893.1 At2g32700/F24L7.16
{Arabidopsis thaliana}, partial (59%)
Length = 1824
Score = 31.6 bits (70), Expect = 0.44
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +2
Query: 248 FSHDRKFLGSIGHDQMLKLWDLDNILQGSRSTQRNETGVVAN 289
FS D K L S GHD+ + LW+++ + ++ST T ++ +
Sbjct: 824 FSSDGKLLASAGHDKKVVLWNMETL--KTQSTPEEHTVIITD 943
>TC87605 homologue to PIR|G96563|G96563 probable coatomer complex subunit
33791-27676 [imported] - Arabidopsis thaliana, partial
(23%)
Length = 765
Score = 31.6 bits (70), Expect = 0.44
Identities = 28/111 (25%), Positives = 51/111 (45%), Gaps = 1/111 (0%)
Frame = +3
Query: 12 IDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAARFINGGRAVLT 71
+D HP+E + + L G + ++ Y S T + V E+ R+A+F+ + V+
Sbjct: 186 VDLHPTEPWILASLYSGTVCIWNYQS-QTMAKSFEVTEL-----PVRSAKFVARKQWVVA 347
Query: 72 GSPDFSILATDVETGSTIARLDNAHEAAVNRL-INLTESTVASGDDDGCIK 121
G+ D I + T + ++ AH + + ++ T V S DD IK
Sbjct: 348 GADDMFIRVYNYNTMDKV-KVFEAHTDYIRCVAVHPTLPYVLSSSDDMLIK 497
>TC79260 weakly similar to GP|9759350|dbj|BAB10005.1 WD-repeat protein-like
{Arabidopsis thaliana}, partial (50%)
Length = 1227
Score = 30.8 bits (68), Expect = 0.76
Identities = 13/44 (29%), Positives = 27/44 (60%)
Frame = +2
Query: 227 LPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLD 270
+P+R ++ + H + V + FSHD K+L + +D+ +W++D
Sbjct: 509 IPSRTLQILDAHDD-EVWFVQFSHDGKYLATASNDRTAIIWEVD 637
>TC78591 similar to GP|9759350|dbj|BAB10005.1 WD-repeat protein-like
{Arabidopsis thaliana}, partial (17%)
Length = 916
Score = 30.8 bits (68), Expect = 0.76
Identities = 13/44 (29%), Positives = 27/44 (60%)
Frame = +2
Query: 226 ILPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDL 269
++P+R ++ + H + V + FSH+ K+L S +DQ +W++
Sbjct: 425 LIPSRTVQILETHDD-EVWFVQFSHNGKYLASASNDQTAIIWEV 553
>TC82717 weakly similar to GP|21539583|gb|AAM53344.1 putative coatomer
complex subunit {Arabidopsis thaliana}, partial (29%)
Length = 1118
Score = 30.0 bits (66), Expect = 1.3
Identities = 24/92 (26%), Positives = 40/92 (43%)
Frame = +3
Query: 12 IDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAARFINGGRAVLT 71
+D HP+E V GL G + ++ Y + T ++V E RAA+FI ++
Sbjct: 150 VDLHPTEPWVLLGLYSGTISIWNYQT-KTEEKSLKVSE-----SPVRAAKFIVRENWIIA 311
Query: 72 GSPDFSILATDVETGSTIARLDNAHEAAVNRL 103
+ D I + E I + AH+ + L
Sbjct: 312 ATDDKYIRVYNYEKMEKIIEFE-AHKDYIRSL 404
>BG647724 similar to GP|11141605|gb LEUNIG {Arabidopsis thaliana}, partial
(25%)
Length = 757
Score = 29.3 bits (64), Expect = 2.2
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +2
Query: 248 FSHDRKFLGSIGHDQMLKLWDLDNILQ 274
FS D K L S GHD+ + LW D++ Q
Sbjct: 455 FSSDGKLLASGGHDKKVVLWYTDSLKQ 535
>TC92332 similar to GP|18377648|gb|AAL66974.1 unknown protein {Arabidopsis
thaliana}, partial (34%)
Length = 1164
Score = 28.5 bits (62), Expect = 3.8
Identities = 23/79 (29%), Positives = 41/79 (51%), Gaps = 4/79 (5%)
Frame = +2
Query: 1 MEINLGKLAFDIDFHPSEN-LVASGLIDGDLHLYRYS-SDNTNSDPVRVLEVHAHTESCR 58
M ++ G +DFHPS + L+ G +G++ L+ S + S P ++ +V A + +
Sbjct: 434 MTLHQGSSVTSLDFHPSHHTLLLVGSSNGEITLWELSLRERLVSKPFKIWDVSACSLPFQ 613
Query: 59 AARFINGGRAV--LTGSPD 75
AA + +V +T SPD
Sbjct: 614 AAAVKDAPISVSRVTWSPD 670
>TC88481 similar to PIR|T00806|T02445 probable U4/U6 small nuclear
ribonucleoprotein [imported] - Arabidopsis thaliana,
partial (60%)
Length = 1836
Score = 28.5 bits (62), Expect = 3.8
Identities = 10/26 (38%), Positives = 18/26 (68%)
Frame = +1
Query: 245 RLAFSHDRKFLGSIGHDQMLKLWDLD 270
R+AF K+LG+ +D+ +LWD++
Sbjct: 1216 RIAFHPSGKYLGTASYDKTWRLWDVE 1293
>TC90611 similar to GP|10177223|dbj|BAB10298. contains similarity to
GTP-binding regulatory protein and WD-repeat
protein~gene_id:MPF21.14, partial (40%)
Length = 855
Score = 28.5 bits (62), Expect = 3.8
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +1
Query: 238 HSEYPVERLAFSHDRKFLGSIGHDQMLKLW 267
H V LA S D KFL S+ D+ +K+W
Sbjct: 475 HHVDTVSSLALSKDGKFLYSVSWDRTIKIW 564
>TC80769 similar to GP|11141605|gb|AAG32022.1 LEUNIG {Arabidopsis thaliana},
partial (40%)
Length = 1362
Score = 28.1 bits (61), Expect = 4.9
Identities = 15/58 (25%), Positives = 28/58 (47%)
Frame = +3
Query: 233 EPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLDNILQGSRSTQRNETGVVAND 290
+PI E + + FS L + D+ +++WD+DN R+ + T V++ D
Sbjct: 471 KPILEEHSALITDVRFSASMPRLATSSFDKTVRVWDVDNPGYSLRTFTGHSTSVMSLD 644
>TC78145 similar to GP|15294240|gb|AAK95297.1 AT3g18860/MCB22_3 {Arabidopsis
thaliana}, partial (93%)
Length = 2633
Score = 27.7 bits (60), Expect = 6.4
Identities = 13/36 (36%), Positives = 22/36 (61%), Gaps = 2/36 (5%)
Frame = +3
Query: 259 GHDQMLKLWDLDN--ILQGSRSTQRNETGVVANDGD 292
G D ++ +WDL++ +Q + Q TG+V +DGD
Sbjct: 348 GMDTLVLVWDLNSGEKVQSLKGHQLQVTGIVVDDGD 455
>CB893690 weakly similar to GP|9759025|dbj gene_id:K6A12.9~similar to unknown
protein~sp|O15736 {Arabidopsis thaliana}, partial (59%)
Length = 916
Score = 27.7 bits (60), Expect = 6.4
Identities = 17/69 (24%), Positives = 30/69 (42%), Gaps = 1/69 (1%)
Frame = +2
Query: 50 VHAHTESCRAARFINGGRAVLTGSPDFSILATDVETGSTIARLDNAHEAAVNRLI-NLTE 108
++AH C + F ++TG D S+ D TGS + L + ++ I N
Sbjct: 263 LNAHEGGCASLLFEYNSSKLITGGQDHSVKVWDTNTGSLSSSLTGCLGSVLDLTITNDNR 442
Query: 109 STVASGDDD 117
S +A+ +
Sbjct: 443 SVIAASSSN 469
>TC93456 similar to GP|12711287|emb|CAC28528. GATA-1 zinc finger protein
{Nicotiana tabacum}, partial (6%)
Length = 663
Score = 27.7 bits (60), Expect = 6.4
Identities = 17/53 (32%), Positives = 27/53 (50%)
Frame = -3
Query: 108 ESTVASGDDDGCIKTLLLHLTQ*KYWPQVETGLFLFAIFDEIKSKPNLNFLKM 160
ES++ + DDDGC T ++ L+ Q E+ L +E +S LN K+
Sbjct: 265 ESSLLTADDDGCCSTFIVRLSS-----QNESSPILDTHSNERRSSNLLNLSKL 122
>TC91549 similar to GP|17063189|gb|AAL32989.1 unknown protein {Arabidopsis
thaliana}, partial (31%)
Length = 1499
Score = 27.3 bits (59), Expect = 8.4
Identities = 16/46 (34%), Positives = 29/46 (62%), Gaps = 2/46 (4%)
Frame = -2
Query: 268 DLDNILQGSRSTQRNETG-VVANDGDSDDG-DEMDVDNSASKFSKG 311
+++++L +S NE+G VV + S+ G DE+D+ ++ S SKG
Sbjct: 1219 EVNSLLL*VKSKTYNESGGVVCEEESSETGADELDMSSTVSSLSKG 1082
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.141 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,239,782
Number of Sequences: 36976
Number of extensions: 117167
Number of successful extensions: 752
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 738
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 751
length of query: 332
length of database: 9,014,727
effective HSP length: 97
effective length of query: 235
effective length of database: 5,428,055
effective search space: 1275592925
effective search space used: 1275592925
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148996.16