BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148970.12 + phase: 0
(560 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77597 similar to GP|6041823|gb|AAF02138.1| lysyl-tRNA syntheta... 1124 0.0
TC77594 similar to PIR|T10672|T10672 aspartate--tRNA ligase homo... 99 4e-21
CB065281 similar to SP|Q51422|SYD_ Aspartyl-tRNA synthetase (EC ... 53 3e-07
TC79805 similar to SP|Q9SW96|SYN1_ARATH Asparaginyl-tRNA synthet... 47 3e-05
TC93515 similar to GP|14532626|gb|AAK64041.1 putative lysyl-tRNA... 40 0.002
AW696985 weakly similar to PIR|C96782|C967 unknown protein F22H5... 29 4.2
BI270106 similar to GP|3093802|gb|A sterol carrier protein X {Or... 29 4.2
TC81332 similar to GP|15810167|gb|AAL06985.1 At1g64110/F22C12_22... 28 7.1
AL388904 28 9.3
>TC77597 similar to GP|6041823|gb|AAF02138.1| lysyl-tRNA synthetase
{Arabidopsis thaliana}, partial (86%)
Length = 2157
Score = 1124 bits (2907), Expect = 0.0
Identities = 554/554 (100%), Positives = 554/554 (100%)
Frame = +1
Query: 1 MRSAQENRPAAADEEDMDPTQYLENRLKYLSTEKEEGRNPYPHKFSATMTIKQYIKEYEG 60
MRSAQENRPAAADEEDMDPTQYLENRLKYLSTEKEEGRNPYPHKFSATMTIKQYIKEYEG
Sbjct: 262 MRSAQENRPAAADEEDMDPTQYLENRLKYLSTEKEEGRNPYPHKFSATMTIKQYIKEYEG 441
Query: 61 LSDGQHLDDVSVSLTGKIMHKRSSGAKLVFYDLHDDGFKVQVMADASKSDLNEAGFDKVH 120
LSDGQHLDDVSVSLTGKIMHKRSSGAKLVFYDLHDDGFKVQVMADASKSDLNEAGFDKVH
Sbjct: 442 LSDGQHLDDVSVSLTGKIMHKRSSGAKLVFYDLHDDGFKVQVMADASKSDLNEAGFDKVH 621
Query: 121 SNVKRGDIVGITGFPGKSRKGELSIFPKTFVVLSHCLHMMPRQKSAAAAAADNAWLPGKT 180
SNVKRGDIVGITGFPGKSRKGELSIFPKTFVVLSHCLHMMPRQKSAAAAAADNAWLPGKT
Sbjct: 622 SNVKRGDIVGITGFPGKSRKGELSIFPKTFVVLSHCLHMMPRQKSAAAAAADNAWLPGKT 801
Query: 181 RNPEAYTLKDQETRYRLRHLDLMLNTEVREIFRTRSKIISYVRSFLDKLDFLEVETPMMN 240
RNPEAYTLKDQETRYRLRHLDLMLNTEVREIFRTRSKIISYVRSFLDKLDFLEVETPMMN
Sbjct: 802 RNPEAYTLKDQETRYRLRHLDLMLNTEVREIFRTRSKIISYVRSFLDKLDFLEVETPMMN 981
Query: 241 MIAGGAAARPFVTHHNDLNMRLFMRIAPELYLKKLVVGGLNRVYEIGKQFRNEGIDLTHN 300
MIAGGAAARPFVTHHNDLNMRLFMRIAPELYLKKLVVGGLNRVYEIGKQFRNEGIDLTHN
Sbjct: 982 MIAGGAAARPFVTHHNDLNMRLFMRIAPELYLKKLVVGGLNRVYEIGKQFRNEGIDLTHN 1161
Query: 301 PEFTTCEFYMAYKDYYDLMEITENMLSGMVYELTKGSYKIKYHADGLDKDPIEIDFTPPF 360
PEFTTCEFYMAYKDYYDLMEITENMLSGMVYELTKGSYKIKYHADGLDKDPIEIDFTPPF
Sbjct: 1162PEFTTCEFYMAYKDYYDLMEITENMLSGMVYELTKGSYKIKYHADGLDKDPIEIDFTPPF 1341
Query: 361 RRIDMIEGLEEMAGLSIPKDLTSAEANQYLRDACLKYEIKCPPPETTARLLDKLVGHFLE 420
RRIDMIEGLEEMAGLSIPKDLTSAEANQYLRDACLKYEIKCPPPETTARLLDKLVGHFLE
Sbjct: 1342RRIDMIEGLEEMAGLSIPKDLTSAEANQYLRDACLKYEIKCPPPETTARLLDKLVGHFLE 1521
Query: 421 ETCVNPTFIINHPEIMSPLAKWHRTKPGLTERFELFINKHELANAYTELNDPVVQRQRFA 480
ETCVNPTFIINHPEIMSPLAKWHRTKPGLTERFELFINKHELANAYTELNDPVVQRQRFA
Sbjct: 1522ETCVNPTFIINHPEIMSPLAKWHRTKPGLTERFELFINKHELANAYTELNDPVVQRQRFA 1701
Query: 481 DQLKDRQSGDDEAMALDETFCTALEYGLPPTAGWGLGIDRLTMLLTDSQNIKEVLLFPAM 540
DQLKDRQSGDDEAMALDETFCTALEYGLPPTAGWGLGIDRLTMLLTDSQNIKEVLLFPAM
Sbjct: 1702DQLKDRQSGDDEAMALDETFCTALEYGLPPTAGWGLGIDRLTMLLTDSQNIKEVLLFPAM 1881
Query: 541 KPQDVVSQLNHLQV 554
KPQDVVSQLNHLQV
Sbjct: 1882KPQDVVSQLNHLQV 1923
>TC77594 similar to PIR|T10672|T10672 aspartate--tRNA ligase homolog
F6E21.100 - Arabidopsis thaliana, partial (73%)
Length = 2043
Score = 99.0 bits (245), Expect = 4e-21
Identities = 116/490 (23%), Positives = 204/490 (40%), Gaps = 10/490 (2%)
Frame = +1
Query: 59 EGLSDGQHLDDVSVSLTGKIMHKRSSGAKLVFYDLHDDGFKVQVMADASKSDLNEAGFDK 118
E L+D L++ V + G+ R G K+ F + ++GF VQ + A + DL K
Sbjct: 334 EALNDS--LENKQVLIRGRAQAIRPVGKKMAFLVIRENGFTVQCLVQA-QPDLVSPQMVK 504
Query: 119 VHSNVKRGDIV---GITGFPGKSRKG---ELSIFPKTFVVLSHCLHMMPRQKSAAAAAAD 172
+ + R IV G+ P KG ++ I + +S + +P + AA
Sbjct: 505 FAAALSRESIVDVEGVVSIPAAPIKGATQQIEIQVRKLYCVSKAIPTLPI--NIEDAARS 678
Query: 173 NAWLPGKTRNPEAYTLKDQETRYRLRHLDLMLNTEVREIFRTRSKIISYVRSFLDKLDFL 232
+ + E +Q+TR R LDL + IFR +S++ + R FL F+
Sbjct: 679 EVEIEKAIQAGEQLVRVNQDTRLNFRVLDLRTPAN-QGIFRIQSQVGNVFRQFLLAESFV 855
Query: 233 EVETPMMNMIAGGAAARPFVTHHNDLNMRLFMRIAPELYLKKLVVGGLNRVYEIGKQFRN 292
E+ TP +IAG + V + + +P+L+ + + G RV+EIG FR
Sbjct: 856 EIHTP--KLIAGSSEGGAAVFRLDYKGQPACLAQSPQLHKQMSICGDFGRVFEIGPVFRA 1029
Query: 293 E-GIDLTHNPEFTTCEFYMAY-KDYYDLMEITENMLSGMVYEL-TKGSYKIKYHADGLDK 349
E H EFT + M K Y+++M++ + + M L TK ++ A+
Sbjct: 1030EDSFTHRHLCEFTGLDVEMEIKKHYFEVMDVVDKLFVAMFDTLNTKCKKDLEAVANQYPF 1209
Query: 350 DPIEIDFTPPFRRIDMIEGLEEMAGLSIPKDLTSAEANQYLRDACLKYEIKCPPPETTAR 409
+P++ + P R+ EG+ Q L++ ++ E R
Sbjct: 1210EPLK--YLPNTLRLTYEEGI------------------QMLKEVGVEIEPFGDLNTEAER 1329
Query: 410 LLDKLVGHFLEETCVNPTFIINHPEIMSPLAKWH-RTKPGLTERFELFINKHELANAYTE 468
L +LV LE+ + +P + P P + F++FI E+ +
Sbjct: 1330KLGQLV---LEKYGTEFYILHRYPMAVRPFYTMPCYDNPNYSNSFDVFIRGEEIISG--- 1491
Query: 469 LNDPVVQRQRFADQLKDRQSGDDEAMALDETFCTALEYGLPPTAGWGLGIDRLTMLLTDS 528
QR + L++R + + + YG+PP G+G+G++R+ ML
Sbjct: 1492-----AQRIHVPEFLEERAQACGIEVKTISAYIDSFRYGVPPHGGFGVGLERVVMLFCGL 1656
Query: 529 QNIKEVLLFP 538
NI++ L+P
Sbjct: 1657NNIRKTSLYP 1686
>CB065281 similar to SP|Q51422|SYD_ Aspartyl-tRNA synthetase (EC 6.1.1.12)
(Aspartate--tRNA ligase) (AspRS). {Pseudomonas
aeruginosa}, partial (32%)
Length = 599
Score = 53.1 bits (126), Expect = 3e-07
Identities = 51/177 (28%), Positives = 85/177 (47%), Gaps = 2/177 (1%)
Frame = -2
Query: 65 QHLDDVSVSLTGKIMHKRSSGAKLVFYDLHDDGFKVQVMADASKSDLNEAGFDKVHSNVK 124
+ LD V+L G + +R G ++F D+ D QV+ D +++ A D+V S
Sbjct: 538 ESLDGQEVTLCGWVHRRRDHGG-VIFLDIRDREGMAQVVFDPDRAETFAAA-DRVRSEY- 368
Query: 125 RGDIVGITGFPGKSRKGELS--IFPKTFVVLSHCLHMMPRQKSAAAAAADNAWLPGKTRN 182
+V ITG K G ++ + +L + L+++ + A P N
Sbjct: 367 ---VVQITGKVRKRPDGAVNANMASGAIEILGYQLNVL-----------NEAETPPFPLN 230
Query: 183 PEAYTLKDQETRYRLRHLDLMLNTEVREIFRTRSKIISYVRSFLDKLDFLEVETPMM 239
Y+ +ETR R R +DL E+ + R RS+I S +R +LD+ FL+VETP++
Sbjct: 229 E--YSDVGEETRLRYRFIDLR-RPEMADKLRLRSRITSSIRRYLDENGFLDVETPIL 68
>TC79805 similar to SP|Q9SW96|SYN1_ARATH Asparaginyl-tRNA synthetase
cytoplasmic 1 (EC 6.1.1.22) (Asparagine-- tRNA ligase 1)
(AsnRS 1)., partial (54%)
Length = 1432
Score = 46.6 bits (109), Expect = 3e-05
Identities = 68/291 (23%), Positives = 121/291 (41%), Gaps = 15/291 (5%)
Frame = +2
Query: 263 FMRIAPELYLKKLVVGGLNRVYEIGKQFRNEGIDLT-HNPEFTTCEFYMAYKDYYDLMEI 321
F+ ++ +L ++ L+RVY G FR E + H EF E +A+ D D M+
Sbjct: 149 FLTVSGQLQVESYACA-LSRVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMKC 325
Query: 322 TENMLSGMVYELTKGSYK-IKYHADGLDK---DPIEIDFTPPFRRI---DMIEGLEEMA- 373
E + + L + +++ AD DK D +++ + PF R+ + +E LEE
Sbjct: 326 AEAYVKFLCRWLLDNCLEDMEFMADKFDKGCIDRLKMVASTPFIRLTYTEAVEILEESVQ 505
Query: 374 -GLSIPKDLT-----SAEANQYLRDACLKYEIKCPPPETTARLLDKLVGHFLEETCVNPT 427
G K++ ++E +YL + +KY+ P
Sbjct: 506 NGKKFEKEVEWGIDLASEHERYLTE--VKYQ--------------------------KPV 601
Query: 428 FIINHPEIMSPLAKWHRTKPGLTERFELFINKHELANAYTELNDPVVQRQRFADQLKDRQ 487
+ N+P+ + A + R L + E L EL + +R+ D ++ R
Sbjct: 602 IVYNYPKDIK--AFYMR----LNDDLETVAAMDVLVPKVGELIGGSQREERY-DVIQQRL 760
Query: 488 SGDDEAMALDETFCTALEYGLPPTAGWGLGIDRLTMLLTDSQNIKEVLLFP 538
D + E + YG AG+GLG +R+ + T +NI++V+ FP
Sbjct: 761 EEMDLPVEPYEWYIDLRRYGTVKHAGFGLGFERMILFATGLENIRDVIPFP 913
>TC93515 similar to GP|14532626|gb|AAK64041.1 putative lysyl-tRNA synthetase
{Arabidopsis thaliana}, partial (27%)
Length = 414
Score = 40.0 bits (92), Expect = 0.002
Identities = 24/88 (27%), Positives = 46/88 (52%), Gaps = 2/88 (2%)
Frame = +1
Query: 5 QENRPAAADEEDMDPTQYLENRLKYLSTEKEEGRNPYPHKFSATMTIKQYIKEYEGLSDG 64
+ N P+++ D RLK + + +G NPY ++++ T + Q Y+ L +G
Sbjct: 154 RRNSPSSSTTSTSDRESIRAMRLKKVEELRSKGHNPYAYEWNKTHSANQLQDIYKDLGNG 333
Query: 65 QHLDDVS--VSLTGKIMHKRSSGAKLVF 90
+ + + VS+ G+I+ +R+ G KL F
Sbjct: 334 EETNSENDHVSIAGRIIARRAFG-KLAF 414
>AW696985 weakly similar to PIR|C96782|C967 unknown protein F22H5.13
[imported] - Arabidopsis thaliana, partial (14%)
Length = 736
Score = 29.3 bits (64), Expect = 4.2
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = -3
Query: 140 KGELSIFPKTFVVLSHCLHMMPRQKSAAAAAADNAWLP 177
+ LS P T +V S CL++ PR + +A AD +W P
Sbjct: 677 RSSLSHCPTTLLVCSCCLYL-PRVSARSARIADPSWRP 567
>BI270106 similar to GP|3093802|gb|A sterol carrier protein X {Oryctolagus
cuniculus}, partial (3%)
Length = 672
Score = 29.3 bits (64), Expect = 4.2
Identities = 28/97 (28%), Positives = 42/97 (42%)
Frame = +2
Query: 5 QENRPAAADEEDMDPTQYLENRLKYLSTEKEEGRNPYPHKFSATMTIKQYIKEYEGLSDG 64
+EN ++ DMD Y + K + TE+E + S IK +K G +G
Sbjct: 320 EENYVDVEEQNDMDNRSYDDLIKKIIGTEEELRVTNSKLRLSEEENIK--LKVQVGNGEG 493
Query: 65 QHLDDVSVSLTGKIMHKRSSGAKLVFYDLHDDGFKVQ 101
Q LD+VS L K R L ++L D+ + Q
Sbjct: 494 Q-LDNVSEELNLKKKELRKQKELLXEFNLKDEELQKQ 601
>TC81332 similar to GP|15810167|gb|AAL06985.1 At1g64110/F22C12_22
{Arabidopsis thaliana}, partial (39%)
Length = 1514
Score = 28.5 bits (62), Expect = 7.1
Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 5/49 (10%)
Frame = +1
Query: 316 YDLMEITENMLSGMVYELTKGSYKIKYHADGLDKD-----PIEIDFTPP 359
Y L + T N LSG V L Y +KY+ +++ P I+ TPP
Sbjct: 199 YRLFQKTLNKLSGSVLILGSRPYDLKYNCTKVNEKLTMLFPYNIEITPP 345
>AL388904
Length = 428
Score = 28.1 bits (61), Expect = 9.3
Identities = 14/27 (51%), Positives = 17/27 (62%)
Frame = -2
Query: 113 EAGFDKVHSNVKRGDIVGITGFPGKSR 139
E+ F++VHSNVKR I T P K R
Sbjct: 304 ESRFNQVHSNVKRKQIQTFTTQPQKKR 224
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.136 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,389,635
Number of Sequences: 36976
Number of extensions: 192020
Number of successful extensions: 867
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 863
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 867
length of query: 560
length of database: 9,014,727
effective HSP length: 101
effective length of query: 459
effective length of database: 5,280,151
effective search space: 2423589309
effective search space used: 2423589309
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC148970.12