BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148969.8 - phase: 0
(219 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86061 similar to GP|19911583|dbj|BAB86895. syringolide-induced... 31 0.42
BG644721 similar to PIR|E84589|E84 probable retroelement pol pol... 28 2.7
TC89142 similar to GP|9759320|dbj|BAB09687.1 gb|AAF00669.1~gene_... 28 3.6
TC92945 similar to GP|22137072|gb|AAM91381.1 At1g42440/F7F22_7 {... 28 3.6
TC91869 similar to PIR|T48903|T48903 wax synthase [imported] - S... 27 4.7
TC78189 homologue to GP|14485513|emb|CAC42087. putative NAC doma... 27 4.7
AJ498708 similar to GP|21592967|gb putative GDSL-motif lipase/ac... 27 4.7
BQ158057 27 4.7
TC83268 weakly similar to GP|21592974|gb|AAM64923.1 proline-rich... 27 6.1
TC89303 similar to PIR|T51951|T51951 gamma-adaptin 1 [imported] ... 27 8.0
TC79251 similar to GP|21555541|gb|AAM63881.1 unknown {Arabidopsi... 27 8.0
>TC86061 similar to GP|19911583|dbj|BAB86895. syringolide-induced protein
B15-3-5 {Glycine max}, partial (65%)
Length = 1442
Score = 30.8 bits (68), Expect = 0.42
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +2
Query: 100 ETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIE 140
ET S+ YH+ ++HI+ D+ C G FP F+E
Sbjct: 35 ETSSNAMLSYHEKGFAYIHIFYWDIFCIGRLRIPFP*NFLE 157
>BG644721 similar to PIR|E84589|E84 probable retroelement pol polyprotein
[imported] - Arabidopsis thaliana, partial (1%)
Length = 735
Score = 28.1 bits (61), Expect = 2.7
Identities = 23/64 (35%), Positives = 32/64 (49%), Gaps = 2/64 (3%)
Frame = +1
Query: 101 TFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVS--ANPWVSFTSFDL 158
T+ L + H D R F YSQD C L+ + N +FV+ +P VSFT DL
Sbjct: 415 TYQILSLDNHKDNRSFQPKYSQDYVCNNIFLS-----DVTNSYFVAPPKSPNVSFT--DL 573
Query: 159 NVAN 162
+ +N
Sbjct: 574 SFSN 585
>TC89142 similar to GP|9759320|dbj|BAB09687.1
gb|AAF00669.1~gene_id:MBL20.10~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (26%)
Length = 1525
Score = 27.7 bits (60), Expect = 3.6
Identities = 10/36 (27%), Positives = 20/36 (54%)
Frame = -3
Query: 74 RMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEY 109
R + G++ I+ S+ PC TVF+ + W+++
Sbjct: 1490 RYSQDKGKMAIFPSIGPCRTVFNWCSSEIPDTWNDF 1383
>TC92945 similar to GP|22137072|gb|AAM91381.1 At1g42440/F7F22_7 {Arabidopsis
thaliana}, partial (13%)
Length = 763
Score = 27.7 bits (60), Expect = 3.6
Identities = 14/36 (38%), Positives = 16/36 (43%)
Frame = -1
Query: 100 ETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFP 135
E F+ LW H R F YS VAC L +P
Sbjct: 283 EPFNILWIIEHVSHRSFTF*YSLGVACQNNTLKDYP 176
>TC91869 similar to PIR|T48903|T48903 wax synthase [imported] - Simmondsia
chinensis, partial (14%)
Length = 681
Score = 27.3 bits (59), Expect = 4.7
Identities = 14/50 (28%), Positives = 22/50 (44%)
Frame = +3
Query: 17 HRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARL 66
++K+H S + TY + I A L + K F+P F+ L L
Sbjct: 399 YKKDHESKSNSKSTLTYASATMIIILALLIPLYSKKENFHPKFVLFLYSL 548
>TC78189 homologue to GP|14485513|emb|CAC42087. putative NAC domain protein
{Solanum tuberosum}, partial (56%)
Length = 1556
Score = 27.3 bits (59), Expect = 4.7
Identities = 21/63 (33%), Positives = 31/63 (48%), Gaps = 2/63 (3%)
Frame = +2
Query: 109 YHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVAN--MDNF 166
+H D H+ S DV C + + PK N F + ++P VS F LN+ + D+
Sbjct: 722 FHTDSSGSDHVVSPDVTCDNKEVQSEPKW---NEFVLGSDP-VSAFDFQLNLMDDAEDDP 889
Query: 167 FAP 169
FAP
Sbjct: 890 FAP 898
>AJ498708 similar to GP|21592967|gb putative GDSL-motif lipase/acylhydrolase
{Arabidopsis thaliana}, partial (27%)
Length = 568
Score = 27.3 bits (59), Expect = 4.7
Identities = 10/27 (37%), Positives = 18/27 (66%)
Frame = +1
Query: 8 YTTVDISQWHRKEHFEAFQSVAQCTYN 34
Y++++ + W R EH+ +QS+ QC N
Sbjct: 439 YSSLNST*WMRDEHYLYYQSLLQCKSN 519
>BQ158057
Length = 449
Score = 27.3 bits (59), Expect = 4.7
Identities = 20/55 (36%), Positives = 27/55 (48%), Gaps = 3/55 (5%)
Frame = +1
Query: 82 LVIWDSVHPCYTVFHE---QTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAY 133
L IW+S HP Y ++HE Q T S + +R HIY DV Y + +Y
Sbjct: 280 LSIWNSHHPVY-IYHERR*QDYTISYI-------YRSLSHIY--DVRLYRRSTSY 414
>TC83268 weakly similar to GP|21592974|gb|AAM64923.1 proline-rich protein
putative {Arabidopsis thaliana}, partial (40%)
Length = 1156
Score = 26.9 bits (58), Expect = 6.1
Identities = 25/88 (28%), Positives = 36/88 (40%), Gaps = 8/88 (9%)
Frame = +1
Query: 11 VDISQWHR-----KEHFEAFQSVAQCTYNQTVQLDITAFLKTV---KKNKHKFYPAFIHI 62
+ WH K+ F+ + Q + L T LK + K+ K A +
Sbjct: 526 ITTKNWHDVYFNYKQFFQEAELFIQTYMTP*LALSNTLSLKNMVSRKQTKDVVELAHLK* 705
Query: 63 LARLMNAHPEFRMAMKDGELVIWDSVHP 90
L +M++HP A K V WDSVHP
Sbjct: 706 LHCVMSSHPVCDDASK---YVFWDSVHP 780
>TC89303 similar to PIR|T51951|T51951 gamma-adaptin 1 [imported] -
Arabidopsis thaliana, partial (21%)
Length = 1257
Score = 26.6 bits (57), Expect = 8.0
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = +2
Query: 28 VAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIH 61
V C Q +++DI FLKT +K + YP++++
Sbjct: 395 VCNCF*EQLLEVDIQ-FLKTTRKPTNNSYPSYLY 493
>TC79251 similar to GP|21555541|gb|AAM63881.1 unknown {Arabidopsis
thaliana}, partial (56%)
Length = 1253
Score = 26.6 bits (57), Expect = 8.0
Identities = 17/50 (34%), Positives = 24/50 (48%)
Frame = +2
Query: 134 FPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKV 183
FP GF+ N+F S P S +F N + +F F + YYT G +
Sbjct: 227 FPAGFLSNLFDASRLP--SNRNFSTNY--IISFRDHTFAVPNYYTVGTSI 364
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.137 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,184,633
Number of Sequences: 36976
Number of extensions: 121623
Number of successful extensions: 778
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 777
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 777
length of query: 219
length of database: 9,014,727
effective HSP length: 92
effective length of query: 127
effective length of database: 5,612,935
effective search space: 712842745
effective search space used: 712842745
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC148969.8